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Team:PEA Exeter NH/Notebook Raw
From 2014hs.igem.org
Starting in the 2013 Winter term, our team learned laboratory techniques and protocols. We began research on the effects of fracking and the chemical characteristics of toxic water contaminants: Catechol, naphthalene, and lead. Over the spring semester of 2014, six full time team members were working tirelessly on the project. Here, we present a summary of our daily progress and experiments.
GROWING CELL CULTURES Names: Christina S, Janice C, Melody N Date: April 29th, 2014 Species: E. Coli Part number: BBa_K118021, BBa_J61051, BBa_K518010, BBa_K592009 Results: TBA PICKING CELL CULTURES Names: Alex S, Reginald L, Cat O, Melody N, Janice C, Connie C, Christina S Date: April 30th, 2014 Species: E. Coli Part number and plasmid: BBa_K118021, BBa_J61051, BBa_K518010, BBa_K592009, pSB1C3 Results: TBA RESTRICTION DIGEST Names: Alex S, Reginald L, Cat O, Melody N, Janice C, Connie C, Christina S Date: May 18th, 2014 Species: E. Coli Restricted: BBa_J61051, BBa_K118021 Results: TBA Incubate 20 hours at 37°C GROWING CELL CULTURE Names: Christina S Date: May 20th, 2014 Species: E. Coli PREPARATION OF ANTIBIOTIC SOLUTION Names: Christina S Date: May 20th, 2014 Antibiotic: Chloramphenicol MINIPREP Names: Alex S, Janice C, Melody N Date: May 21st, 2014 Mini prep: BBa_K518010_pSB1C3, BBa_J61051_pSB1C3 Species: E. Coli LIGATION Names: Charis E, Reginald L, Christina S, Cat O Date: May 21st, 2014 Species: E. Coli Results: TBA Tube 1- BBa_J61051_BBa_K118021_pSB1C3
