Team:PEA Exeter NH/Notebook Raw

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Lab Notebook

Starting in the 2013 Winter term, our team learned laboratory techniques and protocols. We began research on the effects of fracking and the chemical characteristics of toxic water contaminants: Catechol, naphthalene, and lead. Over the spring semester of 2014, six full time team members were working tirelessly on the project. Here, we present a summary of our daily progress and experiments.

GROWING CELL CULTURES
Names: Alex S, Reginald L, Cat O, Melody N, Janice C, Connie C, Christina S
Date: April 19th, 2014
Species: E.Coli
Part Number: BBa_J04500, BBa_J04650
Results: TBA

LIGATION
Names: Alex S, Reginald L, Cat O, Melody N, Janice C, Connie C, Christina S
Date: April 20th, 2014
Species: E.Coli
Part Number and plasmid: BBa_J04500, BBa_J04650, pSB1C3
Results: TBA

TRANSFORMATION
Names: Alex S, Reginald L, Cat O, Melody N, Janice C, Connie C, Christina S
Date: April 24th, 2014
Antibiotic: Amp and Kan
Part number and plasmid: BBa_J04500, BBa_J04650, pSB1C3
Results: TBA

GROWING CELL CULTURES
Names: Christina S, Janice C, Melody N
Date: April 29th, 2014
Species: E. Coli
Part number: BBa_K118021, BBa_J61051, BBa_K518010, BBa_K592009
Results: TBA

PICKING CELL CULTURES
Names: Alex S, Reginald L, Cat O, Melody N, Janice C, Connie C, Christina S
Date: April 30th, 2014
Species: E. Coli
Part number and plasmid: BBa_K118021, BBa_J61051, BBa_K518010, BBa_K592009, pSB1C3
Results: TBA

RESTRICTION DIGEST
Names: Alex S, Reginald L, Cat O, Melody N, Janice C, Connie C, Christina S
Date: May 18th, 2014
Species: E. Coli
Restricted: BBa_J61051, BBa_K118021
Results: TBA
Incubate 20 hours at 37°C

GROWING CELL CULTURE
Names: Christina S
Date: May 20th, 2014
Species: E. Coli

PREPARATION OF ANTIBIOTIC SOLUTION
Names: Christina S
Date: May 20th, 2014
Antibiotic: Chloramphenicol

MINIPREP
Names: Alex S, Janice C, Melody N
Date: May 21st, 2014
Mini prep: BBa_K518010_pSB1C3, BBa_J61051_pSB1C3
Species: E. Coli

LIGATION
Names: Charis E, Reginald L, Christina S, Cat O
Date: May 21st, 2014
Species: E. Coli
Results: TBA
Tube 1- BBa_J61051_BBa_K118021_pSB1C3