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From 2014hs.igem.org

Our stain remover enzyme, subtilisin, is being synthesized with biobrickprefix and suffix restriction site sequences. The gene has been delivered in a plasmid with the terminator sequence also (both with the restrictions site prefix and suffix as found at http://parts.igem.org/Help:Prefix-Suffix) and is depicted below by everything within the parenthesis.

(Promoter + RBS) + (BB-prefix + OmpA + Pro-sequence +Subtilisin + Terminator + BB-Suffix)

•The promoter and RBS are together in biobrick part BBa_J04500, which is the same part used in the practice 3a assembly. •BB-prefix is the Biobrick sequence with the appropriate restriction enzyme sites to conform to the 3A assembly standard. •OmpA is a short signal peptide that coaxes e. coli to secrete the downstream protein out of the cell. •Pro-sequence is a protein that helps guide the folding of the subtilisinenzyme and is part of the natural subtilisin gene •Subtilisin is a serine protease enzyme that degrade other proteins •BB-suffix is the Biobrick sequence with the appropriate restriction enzyme sites to conform to the 3A assembly standard. •The terminator is BBa_B1006