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Notebook Table of Contents
| Leadership Policy | Budget | Letter to Admin | August-December |
| March | April | May | June |
AUGUST-DECEMBER
The founding of the St. Mark's School of Texas iGEM Team required overcoming many administrative and logistical hurdles from funding to lab space. Through garnering the support of the science department, we were fortunate enough to have a laboratory set aside for us
Fundamental Research: Go over http://parts.igem.org/Help:Learn, the folder "Learn" in Dropbox
Applied Research: Organize and store DNA Distribution kit materials, (time permitting) go over pipetting with Mr. Adame
Outreach: brainstorm ideas about getting more Marksmen interested in iGEM, plan to make a brochure, determine a date to display the posters Killian created around the campus Web Development: go over wiki design/layout, ask Mr. Rummel if you all are having trouble
October 2, 2013: Our first meeting will be on October 17th during the 10:30 period. We'll be introducing iGEM, an exciting synthetic biology and genetic engineering competition. October 8, 2013: Hey everyone, before our meeting the 17th, I encourage you all to check out this video that provides a great introduction to iGEM. It talks about an astrobiology project done by Stanford and Brown students for this competition. To give you guys some perspective on what we could do, in the high school division last year, a winning team modified E. Coli bacteria to mass produce the hormone, oxytocin! October 16, 2013: Hey guys, I'd just like to remind you all that our first meeting is tomorrow during the 10:30 period. Unless we have an assembly, we will be talking about iGEM and possible projects we'll be doing. October 18, 2013: Here are the assignments. You can find team wikis at https://igem.org/Team_Wikis?year=2013&division=high_school. Remember that for now, our meetings will be every Thursday during the 10:30 period in Mr. Adame's room. For our next meeting, please come with information about your team's project. Be able to explain what, why, and how. This will help immensely when we start brainstorming ideas for our own iGEM project. October 24, 2013: We had a very productive meeting today. Thanks for spending time thoroughly researching your assigned team. You all knew details of your projects very well! For our next meeting, continue looking into your assigned team and focus primarily on cost and parts. See what BioBricks or other resources they utilized to create their project. This next meeting will be the last one focused solely on what other teams have done in the past. We need to start generating ideas for our own project. You can see how one collegiate team that worked on converting CO into CO2 biologically came up with their idea here: https://2012.igem.org/Team:METU/Brainstorm November 2, 2013: Hey guys, At our next meeting, we'll start brainstorming iGEM projects to pursue. I've seen some great ideas from the leadership applications we've received. Please continue exploring topics we could look into. Science magazines, journals, and blogs are great sources for inspiration. Even the news is a viable source. The World iGEM Collegiate Championship Jamboree is also live right now!! You can check out the live videos or the archives of specific teams. Listening to the presentations should give us some valuable insight. November 6, 2013: Hey all, We have a meeting tomorrow at 10:30. Please bring some iGEM project ideas and if you'd like to apply for a leadership position, remember that the deadline is next Friday, November 15. If you have any questions, please let me know. November 7, 2013: I think Gopal mentioned producing bacteria to create O2 to promote healing in bandages. Well Thomas Jefferson High School studied a similar project last year, check it out here: https://2013hs.igem.org/Team:Jefferson_VA_SciCOS/Project. Notice how they took a simple idea and expanded upon it. Here are some of the ideas we generated today. Mr. Adame also sent us a link to a great article on treating contaminated water. Check it out below! Remember, we don't have to come up with a completely original idea for our project. We can take a project someone else has worked on and modify it. November 10, 2013: Hope you all had a great weekend! Go Lions! I'd just like to update you guys on the direction of our iGEM project. Aarohan, Jeffrey, Nikhil, and I thoroughly considered all of the great ideas you all proposed. This afternoon, we made a final decision; we are pursuing a project centered on probiotic production. After consulting with Dr. Sandeep Burma, we found that creating bacteria that would facilitate enzyme production and promote healing in certain populations would not only have useful applications, but also be practical for us accomplish with the resources we have available. November 12, 2013; Hey guys, We'll be going over gene expression/regulation during Thursday's meeting. To help lay the groundwork, I have sent you all two PDFs from chapters of the AP Biology textbook on the basics of DNA, transcription, and translation (check your e-mail). I hope you all will scan them over. The two chapters go in depth on what you learned in first year biology. If you're thinking about competing in the USA Biology Olympiad in the spring, Campbell's Biology (the text used in AP Biology) is the official textbook and most questions come from material covered in the book. November 17, 2013; Hey guys, Check out this TED talk if you have a chance. Here's a little intro from TED.com: "Once it's created, plastic (almost) never dies. While in 12th grade Miranda Wang and Jeanny Yao went in search of a new bacteria to biodegrade plastic -- specifically by breaking down phthalates, a harmful plasticizer. They found an answer surprisingly close to home." November 20, 2013: If you're interested in iGEM, find some time to go over Unit 1: Biotechnology Techniques on http://www.nature.com/scitable/ebooks/intro-to-biotechnology-techniques-and-applications-16570330/contents. You must know these basic techniques before you can do anything biotechnology related. The tutorial covers restriction enzymes, PCR, and the cloning of expressed genes. If you have any questions, feel free to message me this Thanksgiving break. December 4, 2013: Hey guys, Just a reminder that we are having a regular club meeting tomorrow. We have read through all your applications and have determined the remaining leadership positions for this year's Biology Club and iGEM team. We'll announce those and talk about the direction of our iGEM project. December 11, 2013: Hey guys, We have an important meeting tomorrow. Financial support for our iGEM project is contingent upon your attendance at the next two meetings. But sadly, seniors have a conflict with the meeting time again. Fortunately, Raymond Guo has agreed to lead this meeting. Please try to get to the next two meetings promptly at 10:30. December 18, 2013: Hey guys, We have a really important meeting tomorrow. Please be there promptly at 10:30. Be sure to give a warm welcome to our three incredible guests tomorrow. December 19, 2013: The help and support we have received for our biotechnology initiative is truly incredible. Now with the school's financial backing, we will be able to pursue new frontiers of research and discovery through the iGEM competition. To finalize the details of our project, I've created a new Dropbox folder specifically for the iGEM Team. We will start reviewing literature on probiotics over winter break. If you're on the iGEM team, please be on the lookout for documents placed in the iGEM Dropbox folder along with e-mails regarding our project and your individual roles. So far, I have contracts of commitment from the following guys. You will receive an invitation to join the folder from the e-mail address below. If you'd like me to send the invitation to an alternate e-mail, please let me know. December 21, 2013: I'm really excited to announce that we are now an official iGEM team. You can see our team page and our fellow competitors from around the world at https://igem.org/Team_List?year=2014&division=high_school. Our team name is SMTexas. DEVELOPMENTAL PHASE
WEEK OF MARCH 17
General Meeting: Captain introduces details of iGEM to new members, reveals the project direction to all members, and introduces the creation/makeup of the Committees (30 min), divide the team into groups, have first group meetings, discuss having an additional Tuesday or Wednesday after school meeting
Fundamental Research: Go over http://parts.igem.org/Help:Learn, the folder "Learn" in Dropbox
Applied Research: Organize and store DNA Distribution kit materials, (time permitting) go over pipetting with Mr. Adame
Outreach: brainstorm ideas about getting more Marksmen interested in iGEM, plan to make a brochure, determine a date to display the posters Killian created around the campus Web Development: go over wiki design/layout, ask Mr. Rummel if you all are having trouble
WEEK OF MARCH 24
General Meeting: Captain introduces Gene Designer (20 min)
Fundamental Research: Start designing in Gene Designer by looking at possible promoters & repressors, peruse: http://www.biotechniques.com/news/University-of-Cambridge-team-wins-iGEM-synthetic-biology-competition/biotechniques-180278.html & http://biospot10.blogspot.com/2011/07/igem-grand-prize-2009-biosensors-and.html
Applied Research: Work with the Fundamental Research group to develop a streamlined construct, go over best practices in the lab with Mr. Adame
Outreach: start posting posters, print out 300-400 copies of the brochure depending on cost, plan to distribute the brochures after Friday's Upper School Assembly (April 11)
Web Development: follow iGEM Wiki guidelines and create relevant content, make sure to work on the team page
WEEK OF MARCH 31
General Meeting: presentation by the Fundamental Research Group on their findings & in-development construct
Fundamental Research: work with the Applied Research Group to start constructing the plasmid, continue research
Applied Research: find protocols for implementing the construct, identify problems and consult the Fundamental Research Group
Outreach: brainstorm ideas on promoting the biotechnology further, esp. ideas for getting additional funding, possibly think about hosting parents for a luncheon, e-mail Dr. Burma and any other professors at UTSW for lab tour/advice/guidance
Web Development: document progress and work with the Outreach Group
POSTPONED B/C of ISAS Mandatory Meeting: APRIL 2 Evaluation: Meeting with Mr. Adame, Mr. Rummel, and Committee Leaders
ISAS Commitments will be taken into consideration
CONSTRUCTION PHASE
WEEK OF APRIL 7
General Meeting: Captain speech on the leadership policy, Evaluation of Committees by Captain/Faculty Sponsors
Fundamental Research: Continue research
Applied Research: 3A Assembly
Outreach: Finalize Brochure
Web Development: Continue work on the wiki
Captain seeks new recruits for next year's iGEM Team; reveals ultimate vision of the Project to the Upper School; sends the Biotechnology Initiative brochure to underclassmen
WEEK OF APRIL 14
General Meeting: Committee Meetings
Fundamental Research: Finalize research on genes for the detection of VOC biomarkers
Applied Research: Work with the Fundamental Research Team on finding the DNA sequences, reinforce laboratory skills, calculate potential budget for future years
Outreach: Posters around campus, print out 200 copies of the brochure and distribute to interested science instructors, begin development of a more formal packet of info for the administration
Web Development: Assist Outreach, finalize at least half of the wiki
CANCELED UNTIL CONSTRUCT DEVELOPMENT IS COMPLETE: Plan for a UTSW visit April 18
WEEK OF APRIL 21
General Meeting: Brief presentation by Web Development on Thursday
Fundamental Research: Begin creating construct in Gene Designer if necessary, look for options for the purchase of these sequences from biotech companies, check iGEM Registry for existing BioBricks
Applied Research: Finalize budget, continue refining laboratory skills, assist the Fundamental Research Team
Outreach: Finalize packet for the admininstration, print 10 color copies
Web Development: Finalize wiki with detailed content & designs, brief presentation to the Team on how to add/modify content
WEEK OF APRIL 28
General Meeting: Presentation on established construct by the Fundamental Research Team
Fundamental Research: Order sequences, collaborate with the Applied Research Team to address possible issues during the actual creation of the construct
Applied Research: Collaborate with the Fundamental Research Team to identify room for error
Outreach: Design iGEM wearable gear (t-shirt, sweatshirt, and/or hat) and list cost in a document
Web Development: Address iGEM requirements, including Human Practices
WEEK OF MAY 5: AP EXAMINATIONS
General Meeting: Meeting Cancelled
Fundamental Research:
Applied Research:
Outreach:
Web Development:
WEEK OF MAY 12: AP EXAMINATIONS
General Meeting:
Fundamental Research:
Applied Research:
Outreach:
Web Development:
WEEK OF MAY 19
General Meeting:
Fundamental Research:
Applied Research:
Outreach:
Web Development:
WEEK OF MAY 26
General Meeting: Evaluate progress
Fundamental Research:
Applied Research: Prepare lab space and order necessary materials and do orientation for summer wet lab work and research.
Outreach:
Web Development:
MAY 30: REGULAR REGISTRATION FOR HIGH SCHOOL JAMBOREE CLOSES, JAMOREE ATTENDANCE FEES DUE
WEEK OF JUNE 2
General Meeting: Evaluate progress
Fundamental Research:
Applied Research: Do another 3A Assembly and analyze possibility of Gibson Assembly. Work with Fundamental Research to help identify genes.
Outreach:
Web Development:
JUNE 8: TRACK SELECTION DUE, PROJECT ABSTRACTS DUE, TEAM ROSTERS DUE
WEEK OF JUNE 9
General Meeting: Evaluate progress
Fundamental Research:
Applied Research: Finish and analyze 3A Assembly and finish work with Fundamental Research Committee. Also work with Outreach Committee on Poster.
Outreach:
Web Development:
WEEK OF JUNE 16
General Meeting: Evaluate progress
Fundamental Research:
Applied Research: Work with Outreach Team.
Outreach:
Web Development:
JUNE 20: WIKI FREEZE AT 11:59PM BOSTOM TIME (SEE: WIKI REQUIREMENTS AND SAFETY QUESTIONS)
WEEK OF JUNE 23
Practice presentation, polish poster, submit part to iGEM
JUNE 28: JAMBOREE!
