Human practices travesuras2014.html
From 2014hs.igem.org
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Travesuras 2014
GenetiX, as a student group, participated in the event Travesuras 2014, which had place the 26 of April 2014 inside the installations of Tecnologico de Monterrey, Campus Ciudad de Mexico. The GenetiX station was located in the high-school chemistry laboratory where 4 groups attended, composed around of 25 children each, with ages that ranged between 7 and 16 years. Inside the station there were nine of our members present involving children into science in a fun and enriching way for them. These experiments included de polymerization of “Slime” with color and “Silly Putty”.
Before initiating the experiment, a quick explanation of the
process of polymerization was given. For the polymerization of the
Slime, the kids had the opportunity of adding in plastic cups 10mL
of polyvinyl alcohol and 3 mL of a borax solution in addition with
a drop of colorant. Later, the children agitated the mixture with
wooden sticks until it took the slimy consistence expected. This
procedure was made in groups of between 2 and 3 kids who shared
the slime.
A polymerization of the silly putty was made in the same way in
which the slime was made. In this case, 10 mL of white glue (which
contains the polyvinyl acetate) and 20 mL of saturated borax
solution. The children agitated the mixture until it obtained the
desired consistency. Later, they were able to play with it also in
groups of 2 or 3 children. All the experiments were supervised by
the members of GenetiX in order to prevent any accident and solve
further doubts from the children regarding the experiment.
Finally a DNA demonstration took place. For such, a banana was cut
into pieces and got liquefied in a blender with distilled water
and 2.5 gr of NaCl. It was liquefied until a dense liquid was
achieved and then poured into a coffee filter inside a glass
flask. We left the mixture in filtration for about 10 min until
the flask was half-full. Afterwards 5ml of liquid soap was added
to the pulp in order to break the cell walls and membranes and
free the DNA. Also 3 gr of meat tenderizer and another 3 grs of
sodium bicarbonate were added into the mixture. Once the
ingredients were added, the sufficient amount of cold ethanol was
added in order to form a thin layer above the banana mixture.
Since the DNA is insoluble in ethanol, a whitish layer appears
above the banana mixture. Once the process ended, the final
mixture was shown to the children and a brief explanation of what
DNA is was given.