"
Team:TP CC-SanDiego
From 2014hs.igem.org
Revision as of 17:27, 19 June 2014 by Blackbean1 (Talk | contribs)
<!doctype html> <head> <meta charset="utf-8"> <title>iGEM San Diego</title> <script src="http://code.jquery.com/jquery-1.11.1.min.js"></script> <script> $(function() {
$('a[href*=#]:not([href=#])').click(function() {
if (location.pathname.replace(/^\//,) == this.pathname.replace(/^\//,) && location.hostname == this.hostname) {
var target = $(this.hash);
target = target.length ? target : $('[name=' + this.hash.slice(1) +']');
if (target.length) {
$('html,body').animate({
scrollTop: target.offset().top
}, 1000);
return false;
}
}
});
}); </script> <link href="https://e298d40fc30af56b25c594b5705188b5a91b073c.googledrive.com/host/0B21Mp8HDTzNfU1d2WTR6NlhPeU0" rel="stylesheet" type="text/css"> </head>
<body>
<img src="images/front page diagram white 3.png" width="400px" height="400px"/>
<a href="#header-text">See Statistics</a>
STATISTICS
Statistics that are quite relevant to the nature of this experiment
<img src="images/600px-White_Globe_Icon.png" width="150px" height="150px" />
Toxicity of aflotoxin is 10 times that of hydrocyanic acid and 68 times of arsenic
<img src="images/600px-White_Globe_Icon.png" width="150px" height="150px" />
This disease is the third-leading cause of cancer death globally according to WHO (2008), with about 550,000–600,000 new cases each year.
<img src="images/600px-White_Globe_Icon.png" width="150px" height="150px" />
This disease is the third-leading cause of cancer death globally according to WHO (2008), with about 550,000–600,000 new cases each year
Engineering
E. Coli Capable of Extracelluar Secretion of Mycotoxin Detoxifying Enzymes
<img src="images/bannerphase4 transparent white text.png" width="1070" height="292"/>
Microfungi that produce harmful mycotoxins flourish on improperly-stored nuts, grains, meat, and dairy. They especially thrive in developing countries, where the lack of advanced food storage and mycotoxin exposure causes 40% of the diseases. To lessen the problem, our team engineered E. coli strains using synthetic biology tools to produce chimeric mycotoxin-degrading fungal enzymes, Aflatoxin-Detoxifizyme (ADTZ) and Zearalenone Hydrolase (ZHD101), which are designed to be secreted to extra-cellular space by fusing with secretion signal peptides from alpha-amylase and beta-lactamase. In this study, we have successfully generated synthetic genetic materials to produce four chimeric mycotoxin-detoxifying enzymes. The levels of extracellular secretion is also characterized and analyzed. The project will allow a mass production of detoxification enzymes in cost effective way, preventing the squandering of harvested crops, and limiting mycotoxin-related diseases. Increased access to th ese proteins will have an immense commercial, industrial, agricultural, and health impact.
</body> </html>
