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Welcome to homepage of Team:Shenzhen SZMS! Our project is called E. coli Plant-sitter.

Contents 1 Team 1.1 Attributions 2 Project 3 Lab 4 Modeling 5 Human Practice 6 Fun 7 More Info

Team

Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.

Attributions

Hey here! Check out our 17 members!Team:Shenzhen SZMS/Team Members.

Project

Our project is to create an E. coli Plant-sitter toolkit whose functions include temperature moderation, fungi inhibition, and hormone provision. We aim to achieve smart manipulation of plant growth introduced through synthetic biology. We hope that our toolkit will simplify the process of plant cultivation and make the technology of synthetic biology accessible to people’s daily life.

Read more:Team:Shenzhen SZMS/Project

Lab

Wanna find more about how we bulid our plasmid? Check here:Team:Shenzhen SZMS/Lab

Modeling

In order to assess the efficiency of our E.coli babysitter, we have made math models for the core system in it, EC 1.7.7.2, which functions as to heat up the environment to provide a more suitable temperature for the plant.

EC 1.7.7.2 is basically a ferredoxin-nitrate reductase; with the required substrates nitrate and reduced ferredoxin, EC 1.7.7.2 is able to catalyze the reactions below:

nitrite + H₂O + oxidized ferredoxin ⇄ nitrate + reduced ferredoxin + 2 H⁺

HNO₂ + NH₃ = N₂↑+ 2H

obviously as suggested above,the first reaction is a reversible one; the heat we need is actually from the second reaction since the first one is basically to convert nitrate provided by our special LB into nitrite.On the other hand, the warmth released by the first reaction is so little that it has nearly no effect on the result. Consequently,we make only the math models for the second reaction, whose substrate is nitrate. As calculated, the general warmth that would be released is 334.55 KJ/mol, which is a theoretical datum.

However,considering the fact that we do not have adequate data to carry out our math models, we finally decide to use proportionality instead of accurate data. Both of the following charts are established by Excel.

The first one is a model under the condition that purely only EC 1.7.7.2 is functioning, which means, the concentration of it remains unchanged:

The second one is a model under the condition that RNA Thermometer in the first part of our entire plasmid is functioning to start the heating-up system which is mostly EC 1.7.7.2 in certain extent of external temperature (<27℃). Since it is being manipulated by the other system, the concentration of EC 1.7.7.2 can no long remain unchanged, but changed over time:

General information about EC 1.7.7.2 can be found in the following link: http://www.genome.jp/dbget-bin/www_bget?ec:1.7.7.2.

Human Practice

Come and meet the friend of our E.coli babysitter, E.coli the Fluorescent!

Sincere greeting from the iGem lab in Shenzhen Middle School!

Since all of our E.coli the Fluorescent are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."

Our human practice plan includes:

1. Take part in[http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014.
   Details:Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day
2. Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis.
   Details:Team:Shenzhen SZMS/Human Practice/Laboratory Open Day
3. Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology.
   Details:Team:Shenzhen SZMS/Human Practice/Lectures

Fun

Theme song for our project called E.coli Plant-sitter heals the world! A song that always arises in our minds whenever we think of iGem :)

Find more photos here:Team:Shenzhen SZMS/Gallery!

More Info

• [http://www.shenzhong.net/ school offical website (in Chinese)]
• [http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]
• [http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]