From 2014hs.igem.org
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LAB NOTEBOOK
4/27
This weekend we made stock solutions of antibiotic solutions, prepared sterile LB agar plates supplemented with ampicillin, chloramphenicol, and the combination. We also prepared SOB broth for growing up transformed bacteria.
Consequently, we are ready now to begin assembly of our device.
4/29
Today we transformed competent NEB-10 Beta Cells with pLG575 (secretion plasmid) to have a large supply of plasmid obtainable by mini-prep.
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