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From 2014hs.igem.org

[http://aslanbnguyen.wix.com/igem The Main Site]

Located in the Bay Area, Summit Shasta is a revolutionary school that prefers to encourage students to grow from their mistakes, rather than have students memorize meaningless facts. They push their students to seek out challenges and overcome them, thus the iGem team was born, with help they hope to achieve success in bringing their ideas to life. The team is part of Summit Shasta's first year being around, comprised entirely of eager freshman. Perhaps one day they will revolutionize the world. [http://aslanbnguyen.wix.com/igem Click here for more!]

[http://aslanbnguyen.wix.com/igem Official Team Profile]

Contents 1 The Team 2 Goal 3 Our Project 4 Expanding Your Horizon 5 Poster Presentation at Skyline College 5.1 The Notebook 6 3A Assembly 6.1 Results/Conclusions 6.2 Safety 6.3 Human Practices 6.4 Fun! 7 Sponsors

The Team

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Professors:

Chris Vulpe of UC Berkeley

Nick Kapp of Skyline College

Students:

Albert Liu

Alex Liu

Aslan Nguyen

Brendan Thompson

Dexter Hamilton

Ethan Bull-Vulpe

Jen Co

Jimmy Lujan

Kyra Newcomb

Mitchell Wong

Osama Hanhan

Sydney Huddleston

William McEachen

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Goal

Our goal is to create a CO (Carbon Monoxide) sensor that will detect CO and create a noticeable color. This CO sensor would be more useful than it’s electronic cousin in many ways:

It would not require constant power, this means that you could use it in locations with easy access to power, such as third world countries, underground places/mines, portable/handheld, etc.

It would be small and compact, allowing for easy transportation, meaning it could be carried by people everywhere, providing constant protection. It would create a smell, this means that deaf and blind people would be able to be aware of it, as well as allowing people with headphones or ear protection to sniff out CO.

This means that it would have many uses beyond the ordinary electric, roof-bound, carbon monoxide sensor.

Our Project

We plan to ultimately create a bacteria that would be stimulated by the presence of CO to produce a red hue. The uses of this kind of organism/bacteria would include disaster relief in areas that do not have the access to the equipment needed to monitor the concentration of CO in the area. The goal would be to have the bacteria glow red in the presence of CO, and different colors based on the concentration. For example: It would glow a darker red and/or emit a smell if the level of CO in the air was at fatal levels. It is based upon the percentage of CO in the air. Carbon Monoxide is a fatal gas that is undetectable to humans. In iGEM we plan to use biobricks to make sensors. We plan to construct two sensors, one with the sensor and one with the reporter. The plasmid with the reporter with glow red if the levels of CO are at fatal levels. iGEM also requires outreach which the team has and is doing, the outreach includes teaching, and using a website to convey information.

[http://aslanbnguyen.wix.com/igem#!projects/cm8a Read up here!]

Expanding Your Horizon

On March 15th the team went to Skyline College to be part of the Expanding Your Horizons program. They introduced future high schoolers to the idea of synthetic biology. They hoped to inspire young women to be more interested in the field of science.

Poster Presentation at Skyline College

On April 24th, 2014, the team gave a presentation about their ideas and synthetic biology at Skyline College in the form of a poster presentation.

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The Notebook

Every Tuesday we meet to make progress on our iGEM project. On Thursdays we go to the lab at skyline collage and do laboratory work, at the end of our meetings we discuss the progress we have made, and research new information for our next meeting!

3A Assembly

The team had a blast using the 3A assembly kit. Although with some failures, the team moved on and created a successful transformation!

Results/Conclusions

Over the course of the year we have practiced with bacteria by making transformations and competent cells. Here is a diagram of the project we intend to complete:

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Safety

In the lab at Skyline College we are always supervised by Chris Vulpe (Professor at UC Berkeley), and Nicholas Kapp (Professor at Skyline College).

Human Practices

This CO sensor would be useful in many ways:

1. It would not require constant power, this means that you could use it in locations with easy access to power, such as third world countries, underground places/mines, portable/handheld, etc.

2.It would be small and compact, allowing for easy transportation, meaning it could be carried by people everywhere, providing constant protection.

3. It would create a smell, this means that deaf and blind people would be able to be aware of it, as well as allowing people with headphones or ear protection to sniff out CO. This Means that it would have many uses beyond the ordinary electric, roof-bound, carbon monoxide sensor.

Fun!

Kyra is awesome!

Team Anthem

Sponsors

[http://www.bio-rad.com/ Bio-Rad]

Integrated DNA Technologies

[http://www.skylinecollege.edu/ Skyline College] for Labspace

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