Team:CIDEB-UANL Mexico/math capture
From 2014hs.igem.org
Revision as of 21:15, 20 June 2014 by Oda.ibarra (Talk | contribs)
Capture Module
The capture module is based in the use of NhaS, but as NhaS was not the only gene expressed in the circuit. Also RFP was used as a reporter. It was needed to consider this as a factor. The circuit is shown below:
As it is shown in the circuit, the transcription rate is affected by Uv light. Uv promoters, according to Shuang Li’s team (2006), have less efficiency than constitutive promoters, which is approximately 60% of constitutive promoters.
\begin{equation} \large \frac{d\left [ mRNA \right ]}{dt}=\alpha_{1}\cdot f_{y}-d1\left [ mRNA \right ] \end{equation}
\begin{equation} \large f_{y}=0.6 \end{equation}
It was assumed that “fy”, is the regulatory function that can activate or inhibit the system of given gene; it was assumed 0.6 of the percentage given by Shuang Li’s team. Uv rays activate the promoter, but it is not as efficient as constitutive promoters in the transcription process.