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Team:Shenzhen SZMS/Lab
From 2014hs.igem.org
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| Date | Event | Results | Problem summary |
|---|---|---|---|
| Apr.3 | B0015(chl) B0030(chl) Plasmid Transformation | Done (yet this B0030 is single error) | |
| Apr.4 | Single clone selected from the work on Apr.3 (In order to be pithy, this process is omitted from the table in all the following daily work) | Done | |
| Apr.5 | Plasmid preped and culture restored from the bacterial suspension on Apr.4 (omitted later for pithiness and convenience as well) | Done | |
| Apr.6 | K115016(CHL) K115017(CHL) C0051(CHL) transformation | Done | |
| Apr.2 | J23106(AMP) K082003(CHL，as GFP+LVA) transformation (help offered by Mentor Wang) | Done | |
| May.5 | Digestion: K115016(S/P) C0051(X/P) C0051(E/S) B0015(E/X) Electrophoresis Gel extraction 16sheshidu℃ overnight | Done | |
| May.6 | Product from 5.5（CHL）transformed | Failed | Might be the problem during the plasmid prep |
| May.7 | Plasmid mentioned above repreped | Done | |
| May.13 | Digestion: C0051(E/S) B0015(S/P) K082003(E/S) Electrophoresis Gel extraction 16℃ overnight | Done, C0051-B0015 K082003-B0015 gained | |
| May.14 | Products from 5.13（CHL）all transformed | Done, C0051-B0015 K082003-B0015 gained | |
| May.15 | Gel confirmation (products from 5.14), comparison with gel of B0015 | Done, product longer than the original plasmid | |
| May.27 | Digestion: C0051-B0015(X/P) K115016(S/P) K115017(S/P) Electrophoresis Gel extraction Combination Transformation（CHL） | Done, K115016-C0051-B0015 K115017-C0051-B0015 gained. | |
| May.28 | B0030(AMP) transformation | Done; informed that R0051 requires PCR in order to get the primer of EC 1.7.7.2 | |
| May.29 | Digestion: J23106(S/P) K115016-C0051-B0015(X/P) K115017-C0051-B0015(X/P) Electrophoresis Gel extraction Combination Transformation（AMP） | Done, J23106-K115016-C0051-B0015(27℃CI-protein) J23106-K115017-C0051-B0015（32℃CI-protein） gained. | |
| May.30 | The result of sequencing of C0051-B0015 K082003-B0015 received | Correctly combined | |
| Jun.2 | Digestion: B0030(S/P) K082003-B0015(X/P) Electrophoresis Gel extraction Combination Transformation（AMP） | Done | |
| Jun.8 | The results of sequencing of J23106-K115016-C0051-B0015(27℃CI) and J23106-K115017-C0051-B0015（32℃ CI）received. | Correctly combined | |
| Jun.9 | Digestion:B0030-K082003-B0015(E/S) J23106-K115017-C0051-B0015(E/X) Electrophoresis Gel extraction Combination Transformation（AMP） | Done with transformation; Yet failed in the electrophoretic analysis. | Disconnected |
| Jun.10 | ciF1 ciF2 ciF3: primers to synthesize EC 1.7.7.2 and PEP (additional PEP R0051-B0030-) gained. | ||
| Jun.11 | With the original blue 2X Master Mix PCR round 1,2,3 implemented | Failed; only primer founded in the electrophoretic analysis | |
| Jun.12 | Round 1 from 6.11 repeated in the morning; Round 3 PCR with 2X hi-fi Master Mix at noon | Failed in the morning but done in the afternoon; R0051-B0030-1.7.7.2 recycled product gained. | might be the problem of MIX |
| Jun.13 | Digestion: 27℃ CI（S/P）R0051-B0030-1.7.7.2（X/P） 32℃ CI（E/X） B0030-K082003-B0015(E/S) Electrophoresis Gel extraction Combination Transformation |
