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From 2014hs.igem.org

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	Team FHS_Frederick_MD

Official Team Profile

Contents 1 Team 2 Goals 3 Microbial Fuel Cells 4 Gene Design 4.1 NirB Promoter 4.2 LOV Domain 5 Methods 5.1 3A Assembly 6 Notebook 7 Results/Conclusions 8 Safety 9 Attributions 10 Human Practices 10.1 Renewable Energy 10.2 Clean Water 10.3 Basic Research 10.4 Public Awareness 11 Fun! 12 Sponsors

Team

We are interested in creating a microbial fuel cell that utilizes anaerobic bacteria to produce electricity. In order to optimize the growth conditions in the fuel cell, a fluorescent protein marker will be added so to visualize bacterial growth. We plan to implement an oxygen-sensitive promoter to induce expression of the glowing gene. This should ensure that bacteria only grow under anaerobic conditions. This would lead to the creation of a genetic construct that can be deposited back into the “toolbox” parts repository for iGEM.

Goals

Microbial Fuel Cells

This is about microbial fuel cells. (Alan's section)

A microbial fuel cell is a device that converts the chemical reactions of bacteria into electricity. Within the fuel cell certain bacteria under anaerobic conditions will remove the electrons from organic matter and transfer them to an anode, which will then transfer the electrons through a circuit to a cathode. The current and voltage produced by this process is what creates the electricity required to power certain objects such as a light bulb.

The bacteria that we are currently creating is meant to optimize the microbial fuel cell's potential, as bacteria that will glow under anaerobic conditions will reveal any weaknesses in the fuel cell, structural or otherwise, which can then be assessed and dealt with.

Gene Design

(Kyle and Jonathon, briefly summarize how NirB and LOV work together.)

NirB Promoter

(Kyle, can you add a paragraph here on the NirB promoter?)

LOV Domain

(Jonathon, this is your area to describe how we engineered the LOV gene.)

Methods

(This is Dillon's domain.)

3A Assembly

Notebook

Show us how you spent your days.

Results/Conclusions

What did you achieve over the course of your semester?

Safety

What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?

Attributions

(ALL: Please write something about yourself and what you have been doing on the team.)

	Kyle Andrushko ...
	Dillon Kestner ...
	Jonathon Soward ...
	Alan Nguyen ...
	Mark Trice ...
	David Rozak is a research scientist at the United States Army Medical Research Institute for Infectious Diseases and a founding director of the nonprofit organization, Ars Biotechnica, Inc. Dave has been working with the Frederick High School Bioengineering Club for two years and served as an advisor to our iGEM team.
	Gary Lopez ...

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Human Practices

What impact does/will your project have on the public?

Renewable Energy

(Kyle: How do fuel cell help produce clean renewable energy?)

Clean Water

(Alan: How do fuel cell produce a clean source of water?)

Basic Research

(Dillon: How will the oxygen-sensitive fluorescent gene we're making help people study and improve upon microbial fuel cell performance?)

Public Awareness

(Jonathon: Discuss how our meeting with Cardin helped raise public awareness for STEM.)

Fun!

What was your favorite team snack?? Have a picture of your team mascot?

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Sponsors

We'd like to thank the following organizations for making this possible.