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Team:CIDEB-UANL Mexico/labwork results
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Results
Ligation of NhaS and pSB1C3 (Capture module)
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In the first transformation of the ligation of NhaS and pSB1C3 grew red (expressing RFP) and white bacteria.
Image 1. NhaS first ligation with pSB1C3. Result of the transformation of the ligation between NhaS and pSB1C3.
Image 2.NhaS first ligation with pSB1C3. Result of the transformation of the ligation between NhaS and pSB1C3.
Result of ligation BSMT1 opt. and pSB1C3 (Aroma module)
Image 3.Colonies obtained from the transformation of the ligation BSMT1 opt and pSB1C3.
UV Experimentation
During 2 hours the four Petri dishes were exposed to UV irradiation. None of them showed any change.
Image 4.Petri Dish inoculated with NhaS in pSB1C3 exposed to UV irradiation. Before, no time being exposed (left) and after 2 hours of being exposed (right).
Repetition UV Experimentation
Two Petri dishes inoculated by streak: One with NhaS Red and the other with NhaS White. Both exposed during 30 minutes to UV irradiation and none of them showed any change.
With no time exposed to UV irradiation:
Image 5.Nhas in pSB1C3 before being exposed to UV irradiation.
After 30 minutes:
Image 6.Nhas in pSB1C3 before being exposed to UV irradiation.
Viability in salt (experiment 1)
Bacteria transformed with the capture plasmid were inoculated in Petri dishes with different concentrations of salt
Image 7. All the 18 Petri dishes inoculated with NhaS Red in pSB1C3 of all the 9 used concentrations.
Image 8. All the 18 Petri dishes inoculated with NhaS White in pSB1C3 of all the 9 used concentrations.
Image 9. All the 18 Petri dishes inoculated with the Control bacteria of all the 9 used concentrations.
All the bacteria containing the NhaS in pSB1C3 (Red and White) survived to a 10% concentration of salt.
None of the control group lived in any concentration of salt.
Image 10. Nine Petri dishes with the maximum concentration of salt (10%) used in this experiment. From up to bottom: NhaS Red in pSB1C3, NhaS White in pSB1C3 and the Control bacteria.
Viability in salt. Experiment 2. (Capture module)
As in the Experiment 1, bacteria transformed with the capture plasmid were inoculated in Petri dishes with different concentrations of salt, but this time the mayor concentration is higher (15%)
All the bacteria transformed with NhaS in pSB1C3 (Red and White) lived in the 15% saline medium.
All the control bacteria exposed to any concentration of salt died.
All the control bacteria inoculated only in LB medium (without salt) lived.
Image 11. In the upper left part NhaS Red in pSB1C3 at 15% of salt. In the upper right part NhaS Whit in pSB1C3 at 15% of salt. In the lower left part the Control bacteria at 15% of salt. In the lower right part the Control bacteria with only LB medium (without salt)
Aroma Qualitative Experiment (Aroma module)
Random people were chosen to smell our bacteria. The experiment was performed with three different concentrations of salicylic acid, which were of 10 mM, 20 mM, and 30 mM. All of the samples contained salicylic acid. There was a controlled group grown bellow and above the 32 ºC and a group with transformed bacteria with the Aroma module, also grown bellow and above the 32 ºC; per each concentration.
Those are the words that people used to repeat, or synonyms of what the said, because all the opinions were described in a different way.
Image 12. People smelling the different Petri dishes previously inoculated with the bacteria transformed with the aroma module in order to describe the odor they perceive.
Av. Lázaro Cárdenas to the
East n/a Col. Mederos, Monterrey, Nuevo León, México.
Pedro de Alba n/a Ciudad Universitaria, San Nicolás de Los Garza, Nuevo León, México.
igem.cidebuanl@gmail.com
iGEM CIDEB UANL 2014. CENTRO DE INVESTIGACIÓN Y DESARROLLO DE EDUCACIÓN BILINGÜE. UANL
