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From 2014hs.igem.org

Contents 1 TITLE 2 ABSTRACT 3 BACKGROUD 4 RESULTS AND CONCLUSIONS 4.1 Step 4.1.1 Step1 4.1.2 Step2 4.1.3 Step3 4.1.4 Step4 4.1.5 Step5

TITLE

The UV-responsing tandem generators for a wintergreen odor and protective melittin

ABSTRACT

We design a bacteria that produces a wintergreen odor and protective melittin under extensive UV radiation. The biological system includes two generators: the UV-responsing wintergreen odor generator (BBa_K994000) and UV-responsing melittin generator. The melittin generator is composed of two transcriptional devices: RecA (SOS) promoter (BBa_J22106) and synthesized melittin gene with enterokinase digested sequence. The protective generator takes as input extensive UV radiation and produces as output fusion melittin. Melittin can improve immunity and scavenging free radicals to alleviate the harm from electromagnetic radiation, such as UV and blue light.

BACKGROUD

The electromagnetic spectrum, in order of increasing frequency and decreasing wavelength, can be divided, for practical engineering purposes, into radio waves, microwaves, infrared radiation, visible light, ultraviolet radiation, X-rays and gamma rays. The eyes of various organisms sense a relatively small range of frequencies of EMR called the visible spectrum or light. Higher frequencies (shorter wavelengths) correspond to proportionately more energy carried by each photon. Blue light is the most energetic portion of the visible light spectrum, it's less energetic than UV radiation but it also has the ability to penetrate into tissue and cause cellular damage.

RESULTS AND CONCLUSIONS

Step

Step1

We have to got Part A and Part B's DNA before we digestion. Part A will be used after transformation and miniprep. We got Part B by artificial synthesis.

Step2

Digestion

Step3

Electrophoresis

Step4

Ligation

Step5

Sequencing