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From 2014hs.igem.org

Contents 1 TITLE 1.1 Abstract 1.2 Background 1.3 Step 1.3.1 Step1 1.3.2 Step2 1.3.3 Step3 1.3.4 Step4 1.3.5 Step5 1.3.6 Results/Conclusions

TITLE

The UV-responsing generators for a wintergreen odor and protective melittin

Abstract

We design a bacteria that produces a wintergreen odor and protective melittin under extensive UV radiation. The biological system includes two generators: the UV-responsing wintergreen odor generator (BBa_K994000) and UV-responsing melittin generator. The melittin generator is composed of two transcriptional devices: RecA (SOS) promoter (BBa_J22106) and synthesized melittin gene with enterokinase digested sequence. The protective generator takes as input extensive UV radiation and produces as output fusion melittin. Melittin can improve immunity and scavenging free radicals to alleviate the harm from electromagnetic radiation, such as UV and blue light.

Background

As the students in an international school, we always face many different kinds of assessment events and each of them need to finished by computer. After using Internet whole day, our eyes would feel uncomfortable with innumerable distinct kind radiations. How can we deal with it? The eye is a complex organ, composed of many different structures with varying sensitivity to the effects of radiation. An easy way to look at the eye is to divide it into zones that can be examined with respect to their susceptibility to radiation effects. It is also noteworthy that the effects of radiation on the eye not only depend on the sensitivity of the tissue itself but also on the mode of radiation delivery.

Step

Step1

We have to got Part A and Part B's DNA before we digestion. Part A will be used after transformation and miniprep. We got Part B by artificial synthesis.

Step2

Digestion

Step3

Electrophoresis

Step4

Ligation

Step5

Sequencing

Results/Conclusions

What did you achieve over the course of your semester?