"

From 2014hs.igem.org

	Your team picture
	Team Beijing_HDFLS_High

Contents 1 Project 2 Abstract 3 Background 4 Step 4.1 Step1 4.2 Step2 4.3 Step3 4.4 Step4 4.5 Step5 4.6 Notebook 4.7 Results/Conclusions 4.8 Safety 4.9 Attributions 4.10 Our Team 4.11 Our Leaders 4.12 Zhao ZiDing 4.13 Sukie SunYan 4.14 Ken HuQinKai 4.15 Photographer 4.16 Lisa Hou 4.17 Bookkeepers 4.18 Qian YaTing and Sukie SunYan 4.19 Human Practices 4.20 Fun

Project

Abstract

During consumption of some electronic facilities, such as TV, computer and mobiles, different wave length and frequency of electronic magnetic radiation (EMR) are emitted. EMR has strong penetration, gradually invades human’s body, and embarks different kind of diseases. Melittin is the principal active component of apitoxin (bee venom), consisting of 26 amino acids with the sequence GIGAVLKVLTTGLPALISWIKRKRQQ. Some studies showed that mellitin was resistive to EMR. We are going to construct the bacteria that can produce mellitin.

Background

As the students in an international school, we always face many different kinds of assessment events and each of them need to finished by computer. After using Internet whole day, our eyes would feel uncomfortable with innumerable distinct kind radiations. How can we deal with it? The eye is a complex organ, composed of many different structures with varying sensitivity to the effects of radiation. An easy way to look at the eye is to divide it into zones that can be examined with respect to their susceptibility to radiation effects. It is also noteworthy that the effects of radiation on the eye not only depend on the sensitivity of the tissue itself but also on the mode of radiation delivery.

Step

Step1

We have to got Part A and Part B's DNA before we digestion. Part A will be used after transformation and miniprep. We got Part B by artificial synthesis.

Step2

Digestion

Step3

Electrophoresis

Step4

Ligation

Step5

Sequencing

Notebook

Team Founded

October 14th 2013. We set up our iGem team. In our team we have sixteen people. Everyone voluntary to join our iGem team.

Elected leaders

We let person who wants to be leader go to stair and presented their campaigning, and chose by ourselves.

Brainstorm

To begin with, each of us came up with their own idea of product. Every person had different idea, after listened all person idea. In the end, we chose to use a girl’s idea. Her idea is aim for the Fusion Melittin.

Experiment Design

We found information by searching in Internet and finding in magazines. We made a

Learn process

At first, we studied the Melittin in our club. When we knew what it is and where it comes from, we when to lab to do experiments.

Perfect website

We divided our team to several groups, each group have mission to finish in our web site. Everyone had job to do, we made

Experiment

We went to China Agricultural University to do our experiment. In there, we had teacher to guide us. We learned many things in there.

Results/Conclusions

What did you achieve over the course of your semester?

Safety

Actually we had safety training before we began the experiment. There are something we have to pay attention for it may affect our product. Firstly we have to wash our hands carefully before we touch the chemical reagent. Secondly, we must clean our tools after we finish our experiments. Thirdly, we have to wear the Latex gloves, the white coat, and the masks. Finally, we should read the experiments carefully, and do the steps in order.

Attributions

Our Team

As a team, we believe that the most significant ability for us is the collaboration capabilities, so we did the experiment together. When we were doing experiment, we divided our team members into several groups, and all of us had the chance to experience the professional process with the help of the doctoral students, because of that, we could do the experiment in a proper way. On the other hand, each individual has its own advantages, so we also divide daily work into different parts.

Our Leaders

Zhao ZiDing

A year eleven student, who is the most experienced team member, is our main leader. He always gives us the big direction and share some experience with us.

Sukie SunYan

Sukie SunYan is a year ten student, one of the leaders in our team, she is major in daily transaction.

Ken HuQinKai

KenHuQinKai is a year ten student, he is major in dealing with daily things in our team.

Photographer

Lisa Hou

Lisa is a lovely and calm girl, who is major in taking photos and editing the pictures in our website.

Bookkeepers

Qian YaTing and Sukie SunYan

They are dealing with the money in our team and they control the income and outcome of our team.

Human Practices

Today is a big day for our iGEM team. We visited the Chinese Academy of Sciences Key Laboratory of Plant Molecular Physiology. At first, we met professor Qi Xiaoquan. He analyzed our project with us and give us some helpful suggestions. Then, a Postdoctoral called John Hugh Snyder who is working at here brought us to laboratorys and introduced experimental instruments to us. We have learned how to use experimental instruments.It have a lot of good effects on doing experiments later. It broaden our horizons and experience something I’d never experience before. We were very appreciate to them. File:IMG 0931.jpg File:车上讲解.jpg File:馆内照.jpg File:问问题.jpg File:赵子鼎交流.jpg

Today is May 18th we took on the laboratory curiosity, excitement and a little nervous for the first time to go out to do the experiment . Collection starting at 12:30 in the school Life Science Research Center of Agricultural University to Xiaojiahe .We were divided into four groups each of three individuals along with four graduate students began to do experiments. We do experiments are simple , the first is the plasmid , the rush started with a pipette plus get more drugs than that gets less, still trembling . Although the video saw many times but always imagined yourself different . After completion mention plasmid then do we measure the concentration of enzyme , ran glue. Our group of fairly successful experiment , you can clearly see the results , we have done after finish plastic recycling. Although we set the slowest but we do not have to jump very serious step , the next time I am sure many will be proficient . For the first time into the laboratory is still very excited , everything is still new , and sometimes not always concerned about the experiment , I felt really doing is doing such a thing . In fact, there is another novelty habit of the day, we will eventually have to make sense of everything , as IGEM leaving a full, no regrets footprint.

Fun

We are a very unit team, we like ice-cream and lollipop. Where the crazy is, where we are! Come and support us, it is our pleasure to answer you if anyone ask any question to us.

<forum_subtle />