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From 2014hs.igem.org

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	Team Nanjing_NFLS

Official Team Profile

Contents 1 Team 2 Project 3 Notebook 4 Results/Conclusions 5 Safety 6 Attributions 7 Human Practices 8 Fun!

Team

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Project

Project Description

CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats) are often associated with cas genes which code for proteins that perform various functions related to CRISPRs. The CRISPR-Cas system is a novel technique of gene editing, including silencing, enhancing or changing specific genes. By inserting a plasmid containing cas genes and specifically designed CRISPRs, the organism's genome can be cut at any desired location.

Based on the CRISPR-Cas system, the team plans to design a set of biological machine repair kit including tools to delete and/or replace biobricks in building complex synthetic biology devices and systems. With this repair kit, biological machines malfunction due to mutation and many other causes can be repaired by having one or a few certain biobricks replaced instead of re-building the entire machine.

The procedures include choosing the ideal promoter and terminator with universality for knock-in and designing the DNA sequences for a donor plasmid with homogenous arms and guide-RNA, which then are transfected into the host cells with Cas9. The proof of principle kits will be tested on basic PRCT protein generators with EGFP and RFP as reporters and applied to replace causative genes with non-causative alleles once with proven validity.

Notebook

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Results/Conclusions

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Safety

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Attributions

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Human Practices

What impact does/will your project have on the public?

Fun!

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