LB-BROTH
Materials:
- LB-Broth(chemical storage)
- Sterile dH2O
- Flask
- Lab skale
- Liquid autoclave
- Alumininum folio
- Antibiotic
Experiment:
1. In a steril environment, the tare of the container should be measured and subtracted from the overall weight.
2. 4 grams of LB Broth is put in the container.
3. Add 200 ml distilled water to graduated cyclindar.
4. These two are mixed in a beaker.
5. When you are opening the beaker be sure that it doesn’t contact with air.
6. Autoclave tape is sticked on to the aliminium.
7. The beaker is placed in to the autoclave machine.
8. Autoclave machine has to have distilled water or demineralized water. The water level in the autoclave machine has to be a little higher than the liquid level of the beaker.
9. Then start the 90 min.
10. Take out the beaker and add antibiotics if required.
Attention!
v Don’t be far away fire.
v Don’ forget to write date
LB-Agar
Materials:
- LB-Agar(chemical storage)
- Sterile dH2O
- Flask
- Liquid autoclave
- Aluminium folio
- Antibiotic
- Empty plates
- Parafilm
Experiment:
1. In a steril environment, the tare of the container should be measured and subtracted from the overall weight.
2. 7 grams of LB Agar is put in the container.(If there is no LB agar, add the 4 gram LB broth and 2,7 gram agar.)
3. Add 200 ml distilled water to graduated cyclindar.
4. These two are mixed in a beaker.
5. When you are opening the beaker be sure that it doesn’t contact with air.
6. Autoclave tape is sticked on to the aliminium.
7. The beaker is placed in to the autoclave machine.
8. Autoclave machine has to have distilled water or demineralized water. The water level in the autoclave machine has to be a little higher than the liquid level of the beaker.
9. Then start the 90 min.
10. Take out the beaker and add antibiotics if required.
Attention!
v Don’t be far away fire.
v Don’ forget to write the date.
v Don't forget add the amtibiotics
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