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Team:Nanjing NFLS
From 2014hs.igem.org
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===Project=== | ===Project=== | ||
| - | + | '''Project Description''' | |
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| + | CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats) are often associated with cas genes which code for proteins that perform various functions related to CRISPRs. The CRISPR-Cas system is a novel technique of gene editing, including silencing, enhancing or changing specific genes. By inserting a plasmid containing cas genes and specifically designed CRISPRs, the organism's genome can be cut at any desired location. | ||
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| + | Based on the CRISPR-Cas system, the team plans to design a set of biological machine repair kit including tools to delete and/or replace biobricks in building complex synthetic biology devices and systems. With this repair kit, biological machines malfunction due to mutation and many other causes can be repaired by having one or a few certain biobricks replaced instead of re-building the entire machine. | ||
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| + | The procedures include choosing the ideal promoter and terminator with universality for knock-in and designing the DNA sequences for a donor plasmid with homogenous arms and guide-RNA, which then are transfected into the host cells with Cas9. The proof of principle kits will be tested on basic PRCT protein generators with EGFP and RFP as reporters and applied to replace causative genes with non-causative alleles once with proven validity. | ||
===Notebook=== | ===Notebook=== | ||
Revision as of 10:39, 6 March 2014
| You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing. |  |
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Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs) | File:Nanjing NFLS team.png Your team picture |
| Team Nanjing_NFLS |
| Official Team Profile |
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Contents |
Team
Tell us about your team, your school!
Project
Project Description
CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats) are often associated with cas genes which code for proteins that perform various functions related to CRISPRs. The CRISPR-Cas system is a novel technique of gene editing, including silencing, enhancing or changing specific genes. By inserting a plasmid containing cas genes and specifically designed CRISPRs, the organism's genome can be cut at any desired location.
Based on the CRISPR-Cas system, the team plans to design a set of biological machine repair kit including tools to delete and/or replace biobricks in building complex synthetic biology devices and systems. With this repair kit, biological machines malfunction due to mutation and many other causes can be repaired by having one or a few certain biobricks replaced instead of re-building the entire machine.
The procedures include choosing the ideal promoter and terminator with universality for knock-in and designing the DNA sequences for a donor plasmid with homogenous arms and guide-RNA, which then are transfected into the host cells with Cas9. The proof of principle kits will be tested on basic PRCT protein generators with EGFP and RFP as reporters and applied to replace causative genes with non-causative alleles once with proven validity.
Notebook
Show us how you spent your days.
Results/Conclusions
What did you achieve over the course of your semester?
Safety
What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
Attributions
Who worked on what?
Human Practices
What impact does/will your project have on the public?
Fun!
What was your favorite team snack?? Have a picture of your team mascot?
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