Team:SMTexas
From 2014hs.igem.org
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===Notebook=== | ===Notebook=== | ||
- | + | On Tuesday, February 18th, Vikrant R, Rohin M, and Gopal R, inoculated bacterial strains A & B onto LB agar with ampicillin & kanamycin and incubated overnight at 37C. | |
+ | On Wednesday, February 19th, Vikrant R & Rohin M transferred one colony from each of A & B and inoculated in LB BROTH with ampicillin & kanamycin and placed in shaking water bath at 37C. | ||
+ | |||
+ | The next step is to perform a miniprep, which will allow us to extract plasmid from each A & B bacterial strains, which will then allow us do the restriction digest and ligation. | ||
+ | |||
+ | All of these steps can be seen in the 3A Assembly kit protocols | ||
===Results/Conclusions=== | ===Results/Conclusions=== |
Revision as of 21:58, 19 February 2014
- a team description
- project description
- safety information (did your team take a safety training course? were you supervised in the lab?)
- team attribution (who did what part of your project?)
- lab notebook
- sponsor information
- other information
Example: 2013hs.igem.org/Team:SMTexas/Our_Pets
You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing. | |
Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs) | |
Team SMTexas |
Official Team Profile |
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Contents |
Team
Tell us about your team, your school!
Project
What are you working on this semester?
Notebook
On Tuesday, February 18th, Vikrant R, Rohin M, and Gopal R, inoculated bacterial strains A & B onto LB agar with ampicillin & kanamycin and incubated overnight at 37C.
On Wednesday, February 19th, Vikrant R & Rohin M transferred one colony from each of A & B and inoculated in LB BROTH with ampicillin & kanamycin and placed in shaking water bath at 37C.
The next step is to perform a miniprep, which will allow us to extract plasmid from each A & B bacterial strains, which will then allow us do the restriction digest and ligation.
All of these steps can be seen in the 3A Assembly kit protocols
Results/Conclusions
What did you achieve over the course of your semester?
Safety
What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
Attributions
Who worked on what?
Human Practices
What impact does/will your project have on the public?
Fun!
What was your favorite team snack?? Have a picture of your team mascot?
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