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| - | {{AUC_TURKEY}}
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| |
| - | Name="Table Elegant"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Professional"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Subtle 1"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Subtle 2"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Web 1"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Web 2"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Web 3"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Balloon Text"/>
| |
| - | <w:LsdException Locked="false" Priority="39" Name="Table Grid"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Theme"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" Name="Placeholder Text"/>
| |
| - | <w:LsdException Locked="false" Priority="1" QFormat="true" Name="No Spacing"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 1"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" Name="Revision"/>
| |
| - | <w:LsdException Locked="false" Priority="34" QFormat="true"
| |
| - | Name="List Paragraph"/>
| |
| - | <w:LsdException Locked="false" Priority="29" QFormat="true" Name="Quote"/>
| |
| - | <w:LsdException Locked="false" Priority="30" QFormat="true"
| |
| - | Name="Intense Quote"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="19" QFormat="true"
| |
| - | Name="Subtle Emphasis"/>
| |
| - | <w:LsdException Locked="false" Priority="21" QFormat="true"
| |
| - | Name="Intense Emphasis"/>
| |
| - | <w:LsdException Locked="false" Priority="31" QFormat="true"
| |
| - | Name="Subtle Reference"/>
| |
| - | <w:LsdException Locked="false" Priority="32" QFormat="true"
| |
| - | Name="Intense Reference"/>
| |
| - | <w:LsdException Locked="false" Priority="33" QFormat="true" Name="Book Title"/>
| |
| - | <w:LsdException Locked="false" Priority="37" SemiHidden="true"
| |
| - | UnhideWhenUsed="true" Name="Bibliography"/>
| |
| - | <w:LsdException Locked="false" Priority="39" SemiHidden="true"
| |
| - | UnhideWhenUsed="true" QFormat="true" Name="TOC Heading"/>
| |
| - | <w:LsdException Locked="false" Priority="41" Name="Plain Table 1"/>
| |
| - | <w:LsdException Locked="false" Priority="42" Name="Plain Table 2"/>
| |
| - | <w:LsdException Locked="false" Priority="43" Name="Plain Table 3"/>
| |
| - | <w:LsdException Locked="false" Priority="44" Name="Plain Table 4"/>
| |
| - | <w:LsdException Locked="false" Priority="45" Name="Plain Table 5"/>
| |
| - | <w:LsdException Locked="false" Priority="40" Name="Grid Table Light"/>
| |
| - | <w:LsdException Locked="false" Priority="46" Name="Grid Table 1 Light"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark"/>
| |
| - | <w:LsdException Locked="false" Priority="51" Name="Grid Table 6 Colorful"/>
| |
| - | <w:LsdException Locked="false" Priority="52" Name="Grid Table 7 Colorful"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="46" Name="List Table 1 Light"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark"/>
| |
| - | <w:LsdException Locked="false" Priority="51" Name="List Table 6 Colorful"/>
| |
| - | <w:LsdException Locked="false" Priority="52" Name="List Table 7 Colorful"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 6"/>
| |
| - | </w:LatentStyles>
| |
| - | </xml><![endif]-->
| |
| - | <style>
| |
| - | <!--
| |
| - | /* Font Definitions */
| |
| - | @font-face
| |
| - | {font-family:"Cambria Math";
| |
| - | panose-1:2 4 5 3 5 4 6 3 2 4;
| |
| - | mso-font-charset:1;
| |
| - | mso-generic-font-family:roman;
| |
| - | mso-font-format:other;
| |
| - | mso-font-pitch:variable;
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| |
| - |
| |
| - | <div class=WordSection1>
| |
| - |
| |
| - | <p class=MsoNormal><b><u><span lang=EN-US style='color:red;mso-ansi-language:
| |
| - | EN-US'>Spectrophotometric Absorption Measurements: </span></u></b><span
| |
| - | lang=EN-US style='mso-ansi-language:EN-US'> We used </span><b>Thermo Sc</b><b><span
| |
| - | lang=EN-US style='mso-ansi-language:EN-US'>ientific Varioskan Flash Multimode
| |
| - | Reader for our absorption measurements.</span></b></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>1)
| |
| - | Measurements of Dyes:</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='mso-no-proof:yes'><img width=604 height=440
| |
| - | id="_x0000_i1031" src="https://static.igem.org/mediawiki/2014hs/8/88/Fimage001.jpg"
| |
| - | alt="https://static.igem.org/mediawiki/2014hs/8/88/Fimage001.jpg"></span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>Graphic 1:
| |
| - | This graphic shows our absorption rate of our dye (Methylene Blue) in different
| |
| - | concentrations. ( 1X=8 uM, 10X=80uM, 100X=800uM, 1000X=uM) </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>In the
| |
| - | Graphic 1, we have got the correct absorption rates for every concentration of
| |
| - | Methylene Blue dyes that correlates with the same results in the literature.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>2)
| |
| - | Preparations of Lysates : <o:p></o:p></span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:11.5pt;line-height:115%;font-family:
| |
| - | "Arial","sans-serif";color:black'>The verified plasmids were then cultivated in
| |
| - | liquid culture for isolation. The 50 mL’s of bacteria containing the parts
| |
| - | BBa_K1291071 and BBa_K1291299 were centrifuged at 4100 rpm for 5 minutes, the
| |
| - | supernatant was discarded and then lysis solution was added. After 10 minutes
| |
| - | of incubation in ice, the lysates were sonicated in the minimum activity level.
| |
| - | After a spin of 15000 rpm for 10 minutes, the supernatant was transferred to
| |
| - | eppendorphs and dye was added; the solution was boiled at 95</span></span><span
| |
| - | style='font-size:11.5pt;line-height:115%;font-family:"Arial","sans-serif";
| |
| - | color:#545454; '><span style='orphans: auto;text-align:start;
| |
| - | widows: auto;-webkit-text-stroke-width: 0px;white-space:pre-wrap;word-spacing:
| |
| - | 0px'> °C for 5 minutes. The lysate were stored at -20 °C.</span></span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>3)
| |
| - | Decolorization Assay:</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>In this
| |
| - | assay we mixed our liquid cell culture and also our lysates with dyes. All the
| |
| - | concentrations of the solutions are adjusted according to the literature. We
| |
| - | measured the first absorptions of the samples and compared it with the 2<sup>nd</sup>
| |
| - | measurement in the end of 1<sup>st</sup> hour.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='mso-no-proof:yes'><img width=604 height=300
| |
| - | id="_x0000_i1030" src="https://static.igem.org/mediawiki/2014hs/f/fb/Fimage002.jpg"
| |
| - | alt="https://static.igem.org/mediawiki/2014hs/f/fb/Fimage002.jpg"></span> </p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>Graphic 2:
| |
| - | This graphic shows our absorption rate of our mixed sample that includes
| |
| - | Lysates of our BL-21 Bacteria that have LiP gene and Methylene Blue
| |
| - | (80uM) dyes and for the activate the LiP we also added Hydrogen Peroxide
| |
| - | (800uM) measured in the 1 hour.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>The result
| |
| - | of Graphic-2 is that our Lysates of BL-21 Bacteria that have LiP gene degraded
| |
| - | the Methylene Blue (80uM) dye with approximately %83,3 efficiency.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='mso-no-proof:yes'><img width=605 height=516
| |
| - | id="_x0000_i1029" src="https://static.igem.org/mediawiki/2014hs/7/7d/Fimage003.jpg"
| |
| - | alt="https://static.igem.org/mediawiki/2014hs/7/7d/Fimage003.jpg"></span><span
| |
| - | lang=EN-US style='mso-ansi-language:EN-US'>Graphic 3: This graphic shows our
| |
| - | absorption rate of our mixed samples that includes Lysates of our BL-21
| |
| - | Bacteria that have HRP gene and other Bacteria that have LiP gene and Methyl
| |
| - | Green (150uM) dyes and for the activate the HRP and LiP we also added Hydrogen
| |
| - | Peroxide (8.8 uM-HRP), (0,176 uM-LiP) concentrations that measured in the
| |
| - | different time periods. </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><u><span lang=EN-US style='color:red;mso-ansi-language:EN-US'>The
| |
| - | result of Graphic-3 is that our Lysates of BL-21 Bacteria that have HRP gene
| |
| - | and other Bacteria that have LiP gene degraded the Methyl Green (8.8 uM) dye
| |
| - | with approximately %71,4 efficiency in the end of the 7 hours.</span></u></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='mso-no-proof:yes'><img width=475 height=304
| |
| - | id="_x0000_i1028" src="https://static.igem.org/mediawiki/2014hs/e/ed/Fimage004.png"
| |
| - | alt="https://static.igem.org/mediawiki/2014hs/e/ed/Fimage004.png"></span><span
| |
| - | lang=EN-US style='mso-ansi-language:EN-US'>.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>Graphic 4:
| |
| - | This graphic shows our absorption rate of our mixed samples that includes
| |
| - | Lysates of our BL-21 Bacteria that have HRP gene and other Bacteria that have
| |
| - | LiP gene and Methyl Green (150uM) dyes and for the activate the HRP and LiP we
| |
| - | also added Hydrogen Peroxide (8.8 uM-HRP), (0,176 uM-LiP) concentrations that
| |
| - | measured in the beginning of the experiment and in the 1<sup>st</sup> hour. </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>The result
| |
| - | of Graphic-4 is that our Lysates of BL-21 Bacteria that have HRP gene and other
| |
| - | Bacteria that have LiP gene degraded the Methyl Green (8.8 uM) dye with
| |
| - | approximately %71,4 efficiency. <u><span style='color:red'>Also in this graphic
| |
| - | the most important point that our control group BL-21 bacteria that don’t
| |
| - | include our genes of enzymes(HRP or LiP) couldn’t degrade the Methyl Green (8.8
| |
| - | uM) dye.</span></u></span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='mso-no-proof:yes'><img width=610 height=320
| |
| - | id="_x0000_i1027" src="https://static.igem.org/mediawiki/2014hs/a/a9/Fimage005.jpg"
| |
| - | alt="https://static.igem.org/mediawiki/2014hs/a/a9/Fimage005.jpg"></span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>Graphic 5:
| |
| - | This graphic shows our absorption rate of our mixed sample that includes liquid
| |
| - | culture of our BL-21 Bacteria that have LiP gene and Methylene Blue
| |
| - | (80uM) dyes and for the activate the LiP we also added Hydrogen Peroxide
| |
| - | (800uM) measured in the 1 hour.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>The result
| |
| - | of Graphic-5 is that our Liquid cultures of BL-21 Bacteria that have LiP gene
| |
| - | also degraded the Methylene Blue (80uM) dye with approximately %70 efficiency. </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='mso-no-proof:yes'><img width=603 height=359
| |
| - | id="_x0000_i1026" src="https://static.igem.org/mediawiki/2014hs/e/eb/Fimage006.jpg"
| |
| - | alt="https://static.igem.org/mediawiki/2014hs/e/eb/Fimage006.jpg"></span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>Graphic 6:
| |
| - | This graphic shows our absorption rate of our mixed sample that includes liquid
| |
| - | culture of our BL-21 Bacteria that have HRP gene and Methylene Blue
| |
| - | (80uM) dyes and for the activate the HRP we also added Hydrogen Peroxide
| |
| - | (800uM) measured in the 1 hour.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>The result
| |
| - | of Graphic-6 is that our Liquid cultures of BL-21 Bacteria that have LiP gene
| |
| - | also degraded the Methylene Blue (80uM) dye with approximately %65 efficiency.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>The Results
| |
| - | of Graphic 5 and 6 show that our liquid culture of our BL-21 Bacteria that have
| |
| - | HRP gene and other Bacteria that have LiP gene could degraded the Methylene
| |
| - | Blue (80uM) dye but our Lysates of our BL-21 Bacteria that have HRP gene and
| |
| - | other Bacteria that have LiP gene degraded the Methylene Blue (80uM) dye more
| |
| - | efficiently. This results gave us an opinion that when our enzymes have further
| |
| - | interaction with dyes they could degrade much more.</span></p>
| |
| - |
| |
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| - | <p class=MsoNormal><span style='mso-no-proof:yes'><img width=604 height=283
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| - | id="_x0000_i1025" src="https://static.igem.org/mediawiki/2014hs/6/60/Fimage007.jpg"
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| - | alt="https://static.igem.org/mediawiki/2014hs/6/60/Fimage007.jpg"></span></p>
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| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>Graphic 7:
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| - | This graphic shows our absorption rate of our mixed sample that includes liquid
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| - | culture of our Control BL-21 bacteria that don’t have any HRP or LiP gene
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| - | and Methylene Blue (80uM) dyes and for the activate the any peroxidases,
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| - | Control BL-21 bacteria have, we also added Hydrogen Peroxide (800uM) measured
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| - | in the 1 hour.</span></p>
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| - | <p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>The result
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| - | of Graphic-7 is that our Liquid culture of BL-21 Bacteria that don’t have any
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| - | HRP or LiP gene could degrade the Methylene Blue (80uM) dye with approximately
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| - | % 18 efficiency. This result show that Control bacteria can degrade the the
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| - | Methylene Blue (80uM) dye but at the very low percentage when compared with our
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| - | liquid culture BL-21 Bacteria that have HRP gene and other Bacteria that have
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| - | LiP gene so we thought that some peroxidases also can degrade the dyes but our
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| - | enzymes HRP and LiP have very high catalytic ability for the our
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| - | dyes(Methlylene Blue and Methyl Green). These results and graphics show that
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| - | our enzymes are functional and working properly.</span></p>
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