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| - | {{AUC_TURKEY}}
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| - | {{:Vertical:AUC_TURKEY}}
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| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Elegant"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Professional"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Subtle 1"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Subtle 2"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Web 1"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Web 2"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Web 3"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Balloon Text"/>
| |
| - | <w:LsdException Locked="false" Priority="39" Name="Table Grid"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
| |
| - | Name="Table Theme"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" Name="Placeholder Text"/>
| |
| - | <w:LsdException Locked="false" Priority="1" QFormat="true" Name="No Spacing"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 1"/>
| |
| - | <w:LsdException Locked="false" SemiHidden="true" Name="Revision"/>
| |
| - | <w:LsdException Locked="false" Priority="34" QFormat="true"
| |
| - | Name="List Paragraph"/>
| |
| - | <w:LsdException Locked="false" Priority="29" QFormat="true" Name="Quote"/>
| |
| - | <w:LsdException Locked="false" Priority="30" QFormat="true"
| |
| - | Name="Intense Quote"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="61" Name="Light List Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="19" QFormat="true"
| |
| - | Name="Subtle Emphasis"/>
| |
| - | <w:LsdException Locked="false" Priority="21" QFormat="true"
| |
| - | Name="Intense Emphasis"/>
| |
| - | <w:LsdException Locked="false" Priority="31" QFormat="true"
| |
| - | Name="Subtle Reference"/>
| |
| - | <w:LsdException Locked="false" Priority="32" QFormat="true"
| |
| - | Name="Intense Reference"/>
| |
| - | <w:LsdException Locked="false" Priority="33" QFormat="true" Name="Book Title"/>
| |
| - | <w:LsdException Locked="false" Priority="37" SemiHidden="true"
| |
| - | UnhideWhenUsed="true" Name="Bibliography"/>
| |
| - | <w:LsdException Locked="false" Priority="39" SemiHidden="true"
| |
| - | UnhideWhenUsed="true" QFormat="true" Name="TOC Heading"/>
| |
| - | <w:LsdException Locked="false" Priority="41" Name="Plain Table 1"/>
| |
| - | <w:LsdException Locked="false" Priority="42" Name="Plain Table 2"/>
| |
| - | <w:LsdException Locked="false" Priority="43" Name="Plain Table 3"/>
| |
| - | <w:LsdException Locked="false" Priority="44" Name="Plain Table 4"/>
| |
| - | <w:LsdException Locked="false" Priority="45" Name="Plain Table 5"/>
| |
| - | <w:LsdException Locked="false" Priority="40" Name="Grid Table Light"/>
| |
| - | <w:LsdException Locked="false" Priority="46" Name="Grid Table 1 Light"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark"/>
| |
| - | <w:LsdException Locked="false" Priority="51" Name="Grid Table 6 Colorful"/>
| |
| - | <w:LsdException Locked="false" Priority="52" Name="Grid Table 7 Colorful"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="Grid Table 1 Light Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="Grid Table 6 Colorful Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="Grid Table 7 Colorful Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="46" Name="List Table 1 Light"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark"/>
| |
| - | <w:LsdException Locked="false" Priority="51" Name="List Table 6 Colorful"/>
| |
| - | <w:LsdException Locked="false" Priority="52" Name="List Table 7 Colorful"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 1"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 2"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 3"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 4"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 5"/>
| |
| - | <w:LsdException Locked="false" Priority="46"
| |
| - | Name="List Table 1 Light Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="51"
| |
| - | Name="List Table 6 Colorful Accent 6"/>
| |
| - | <w:LsdException Locked="false" Priority="52"
| |
| - | Name="List Table 7 Colorful Accent 6"/>
| |
| - | </w:LatentStyles>
| |
| - | </xml><![endif]-->
| |
| - | <style>
| |
| - | <!--
| |
| - | /* Font Definitions */
| |
| - | @font-face
| |
| - | {font-family:"Cambria Math";
| |
| - | panose-1:2 4 5 3 5 4 6 3 2 4;
| |
| - | mso-font-charset:1;
| |
| - | mso-generic-font-family:roman;
| |
| - | mso-font-format:other;
| |
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| - | <div class=WordSection1>
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| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Overview</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>The most
| |
| - | appropriate scale to deducing the qualitative chance in reporter systems relies
| |
| - | on visual <span class=GramE>data</span>.<a name="_ednref1"></a><a href="#_edn1"
| |
| - | title=""><span style='mso-bookmark:_ednref1'><span class=MsoEndnoteReference>[i]</span></span><span
| |
| - | style='mso-bookmark:_ednref1'></span></a><span style='mso-bookmark:_ednref1'></span>The
| |
| - | reporter system that we designed as an alternative to the current reporter systems
| |
| - | uses the change factor in the current reporter systems as the response. <span
| |
| - | class=GramE>Instead of observing change through the synthesis of color, the
| |
| - | system is built around the observation of change through breaking down dye; the
| |
| - | dye degradation plays a key role in the system, therefore for the system to
| |
| - | function there is the requirement of using specific dye in experiments
| |
| - | alongside the standard lab equipment. </span>The bacteria will degrade the
| |
| - | necessary dye to report the response.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Color-Based
| |
| - | Reporter System</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>The dye that
| |
| - | was designated and the enzyme that was to show activity in accordance to it was
| |
| - | one of our concerns. The enzyme-substrate action in this novel system carried
| |
| - | great importance and had to be designed with utmost care.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Peroxidases,
| |
| - | which actively take part in the breakdown of lignin-based compounds thus
| |
| - | contributing to the maintenance of the continuity of the existence of white rot
| |
| - | fungi that we based our bleaching enzymes <span class=GramE>on ,were</span>
| |
| - | enzymes that we took into our prospect. We elected lignin peroxidase, an enzyme
| |
| - | actively used by white rot fungi to continue their <span class=GramE>life , as</span>
| |
| - | the enzyme to be implemented into our system. To increase the variance and the
| |
| - | richness in future application, we chose a secondary enzyme. The secondary
| |
| - | enzyme was the horseradish peroxidase as it had a very strong structure and
| |
| - | high activity.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><b><span style='font-size:12.0pt;line-height:115%'>Lignin
| |
| - | peroxidase:</span></b><span style='font-size:12.0pt;line-height:115%'> Lignin
| |
| - | is highly resistant to biodegradation and only higher fungi are capable of
| |
| - | degrading the polymer via an oxidative <span class=GramE>process.Lignin</span>
| |
| - | is found to be degraded by an enzyme lignin peroxidases produced by some fungi
| |
| - | like Phanerochaete chrysosporium.The mechanism by which lignin peroxidase (Lip)
| |
| - | interacts with the lignin polymer which involves Veratryl alcohol (Valc), a
| |
| - | secondary metabolite of white rot fungi, acts as a cofactor for the enzyme.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>In
| |
| - | enzymology, a lignin peroxidase (EC 1.11.1.14) is an enzyme that catalyzes the
| |
| - | chemical reaction</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>1,2-bis(3,4-dimethoxyphenyl)propane-1,3-diol
| |
| - | + H2O2 3,4-dimethoxybenzaldehyde +
| |
| - | 1-(3,4-dimethoxyphenyl)ethane-1,2-diol + H2O </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Thus, the
| |
| - | two substrates of this enzyme are 1,2-bis(3,4-dimethoxyphenyl)propane-1,3-diol
| |
| - | and H2O2, whereas its 3 products are 3,4-dimethoxybenzaldehyde,
| |
| - | 1-(3,4-dimethoxyphenyl)ethane-1,2-diol, and H2O. </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>This enzyme
| |
| - | belongs to the family of oxidoreductases, specifically those acting on a
| |
| - | peroxide as acceptor (peroxidases) and can be included in the broad category of
| |
| - | ligninases. The systematic name of this enzyme class is
| |
| - | 1,2-bis(3,4-dimethoxyphenyl)propane-1,3-<span class=GramE>diol:hydrogen</span>-peroxide
| |
| - | oxidoreductase. Other names in common use include diarylpropane oxygenase,
| |
| - | ligninase I,diarylpropane peroxidase, LiP, <span class=GramE>diarylpropane:oxygen</span>,hydrogen-peroxide
| |
| - | oxidoreductase (C-C-bond-cleaving). It employs one cofactor, heme.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>LiP
| |
| - | catalyzes the H2O2-dependent oxidation of a variety of lignin model compounds
| |
| - | in the following multistep reaction sequence:</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>LiP(Fe+3)P +
| |
| - | H2O2 à LiP-I(Fe+4 – O)P’ + H2O</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>LiP-I(Fe+4 –
| |
| - | O)P’ + R à LiP-II(Fe+4 – O)P + R’</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>LiP-II(Fe+4
| |
| - | – O)P + R + 2(H+) à LiP(Fe+3)P + R’ + H2O </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Horseradish
| |
| - | peroxidase: The enzyme horseradish peroxidase (HRP), found in the roots of
| |
| - | plant horseradish, is used extensively in biochemistry applications primarily
| |
| - | for its ability to amplify a weak signal and increase detectability of a target
| |
| - | molecule. It is a metalloenzyme with many isoforms and the most studied type is
| |
| - | C.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>The
| |
| - | structure of the enzyme was first solved by X-ray crystallography in 1997 and
| |
| - | has since has been solved several times with various substrates. It is an all
| |
| - | alpha-helical protein which binds heme as a cofactor.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Alone, the
| |
| - | HRP enzyme, or conjugates thereof, is of little value; its presence must be
| |
| - | made visible using a substrate that, when oxidized by HRP using hydrogen
| |
| - | peroxide as the oxidizing agent, yields a characteristic change that is
| |
| - | detectable by spectrophotometric methods.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Numerous
| |
| - | substrates for the horseradish peroxidase enzyme have been described and
| |
| - | commercialized to exploit the desirable features of HRP. These substrates fall
| |
| - | into several distinct categories. HRP catalyzes the conversion of chromogenic
| |
| - | substrates (e.g<span class=GramE>.,</span> TMB, DAB, ABTS) into coloured
| |
| - | products, and produces light when acting on chemiluminescentsubstrates (e.g.
| |
| - | ECL).</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Horseradish
| |
| - | peroxidase is a 44,173.9-dalton glycoprotein with 6 lysine residues which can
| |
| - | be conjugated to a labeled molecule. It produces a coloured, fluorimetric, or
| |
| - | luminescent derivative of the labeled molecule when incubated with a proper
| |
| - | substrate, allowing it to be detected and quantified. HRP is often used in
| |
| - | conjugates (molecules that have been joined genetically or chemically) to
| |
| - | determine the presence of a molecular target. For example, an antibody
| |
| - | conjugated to HRP may be used to detect a small amount of a specific protein in
| |
| - | a western blot. Here, the antibody provides the specificity to locate the
| |
| - | protein of interest, and the HRP enzyme, in the presence of a substrate,
| |
| - | produces a detectable <span class=GramE>signal.Horseradish</span> peroxidase is
| |
| - | also commonly used in techniques such as ELISA and Immunohistochemistry due to
| |
| - | its monomeric nature and the ease with which it produces coloured products.
| |
| - | Peroxidase, a heme-containing oxidoreductase, is a commercially important
| |
| - | enzyme which catalyses the reductive cleavage of hydrogen peroxide by an
| |
| - | electron donor.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Horseradish
| |
| - | peroxidase is ideal in many respects for these applications because it is
| |
| - | smaller, more stable, and less expensive than other popular alternatives such
| |
| - | as alkaline phosphatase. It also has a high turnover rate that allows
| |
| - | generation of strong signals in a relatively short time span.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Moreover,
| |
| - | "In recent years the technique of marking neurons with the enzyme
| |
| - | horseradish peroxidase has become a major tool. In its brief history, this
| |
| - | method has probably been used by more neurobiologists than have used the Golgi
| |
| - | stain since its discovery in 1870."</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><b><span style='font-size:12.0pt;line-height:115%'>Design</span></b></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>In order to verify
| |
| - | that the horseradish peroxidase and lignin peroxidase enzymes, which were not
| |
| - | transformated and synthesized in bacteria in the past, worked; we decided to
| |
| - | use a constitutive promoter. We chose the constitutive promoter as J23100, one
| |
| - | of the strongest. We wanted to be certain that our protein is surely
| |
| - | produced due to the J23100 constitutive promoter that is 35 bp and coming after
| |
| - | the prefix of 22 base pairs. <span class=GramE>The exhibition of our results in
| |
| - | Western Blotting through the addition of a 6 histidin long his-tag at the end
| |
| - | of the horseradish peroxidase C and lignin peroxidase 1 enzyme sequences, which
| |
| - | contain BamH-I restriction cites that are followed by our ‘AAAGAGGAGAAA’
| |
| - | sequenced RBS, was added to our lab plans. </span>This would in turn give us
| |
| - | the safest experimental procedure to validate the presence of the proteins
| |
| - | syntesized.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>The accruity
| |
| - | of our design was to be tested through getting the dye to react with the
| |
| - | culture of bacteria containing enzymes that were to be rapidly synthesized
| |
| - | through the constitutive promoter. The changes ranging from the visually
| |
| - | observable changes in the color concentration to the shifts in the wavelenght
| |
| - | absorbance which can only be detected through Varioskan approve the validity of
| |
| - | our project. The degradation of the specified dyes by the lignin peroxidase and
| |
| - | horsereadish peroxidase, enzymes we selected to breakdown these dyes, is enough
| |
| - | to complete the rudimentary part of our project. The addition of the enzymes as
| |
| - | a composite at the end of another part would enable the production of the
| |
| - | protein in the sequence as well as the the enzymes enabling the response
| |
| - | through an accelerated breakdown process.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Aside of the
| |
| - | affirmation of the synthesis of the enzymes with the Western Blott results, to
| |
| - | confirm the functionality of the proteins carries great importance. We came to
| |
| - | the conclusion that working with several methods to verify the results would be
| |
| - | more professional and certain:</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>-The
| |
| - | confirmation of the enzyme activity can be <span class=GramE>done</span>
| |
| - | through collecting the data acquired through the observation of the interaction
| |
| - | between the interaction of the specified dyes and DegradEcolor bacteria. For
| |
| - | this feat to be accomplished, the selection of a dye that both lignin
| |
| - | peroxidase and horseradish peroxidase can degrade in equivalent levels would be
| |
| - | more reasonable. We chose Methylene blue as the dye to be used as it was
| |
| - | degraded by the two enzymes in similar amounts during trials. The trials were
| |
| - | consisting of the observation of the changes in the absorbance and color
| |
| - | through the reactions between methylene blue and the two enzymes. The
| |
| - | degradation of the dye plays a keyrole in the determination of the enzyme
| |
| - | activity<span class=GramE>..</span></span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>-HRP
| |
| - | activity was assayed using 2,4-dichlorophenol and 4-aminoantipyrine as the
| |
| - | substrates, substrates used to assay the activity of enzymes. LiP activity was
| |
| - | assayed using veratryl alcohol as the substrate. </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>-While
| |
| - | examining the general properties of the two designated enzymes, we found out
| |
| - | that the horseradish peroxidase enzyme had the ability to determine the
| |
| - | presence of a molecular target. If we are to recall the Western blott procedure
| |
| - | we see the following:</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>The proteins
| |
| - | in the lysate that were planted in the jel first run and transferred to the
| |
| - | membrane afterwards. After being left to interact with the primary antibodies
| |
| - | causing the immuglobins to bind with the specific binding site located on the <span
| |
| - | class=GramE>protein.In</span> the case of contamination or the absence of
| |
| - | protein synthesis, no binding will be observed. After the completion of the
| |
| - | binding of the primary antibodies, the secondary antibody binding process
| |
| - | begins. While the secondary antibodies bind to the primary antibodies, they
| |
| - | bring with them the horseradish peroxidase enzymes that are attached to them.
| |
| - | The enhanced chemiluminescence solution does emission under the Western
| |
| - | blotting device. The luminol in the enhanced chemiluminescence solution is
| |
| - | oxidised by hydrogen peroxide, however this reaction can only occur in the
| |
| - | presence of the horseradish peroxidase; as explained, if the proteins are
| |
| - | correctly synthesized the horseradish peroxidase will cause oxygenation to take
| |
| - | place causing emission. The same effect can be acquired through the horseradish
| |
| - | peroxidase that is synthesized by DegradEcolor. In thıs case, the functionality
| |
| - | of the enzyme could be proven after seeing emission from the interaction
| |
| - | between the enhanced chemiluminescence solution and DegradEcolor or lysate
| |
| - | prepared from DegradEcolor. As horseradish peroxidase was able to catalise the
| |
| - | reaction of hydrogen peroxide, lignin peroxidase, another peroxidase enzyme,
| |
| - | would most likely be able to have the same effect. We are expecting to see
| |
| - | emission from the bacteria or lysate prepared from the bacteria that produce
| |
| - | lignin peroxidase.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Dye
| |
| - | Degradation</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Methylene blue,
| |
| - | the dye which the horseradish peroxidase and lignin peroxidase exhibited
| |
| - | equivalent levels of degradation on, will show that DegradEcolor produced the
| |
| - | functional proteins. Addition of the horseradish peroxidase or lignin
| |
| - | peroxidase enzyme sequence to the composite part would make the part include a
| |
| - | reporter system. If the part is coded and the enzyme is translated, the enzyme
| |
| - | will be synthesized and change in methylene blue will be observed. Many
| |
| - | deductions can be obtained with the results of the methylene blue degradation
| |
| - | including the following:</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>-The
| |
| - | bleeching of the dye color, which can be understood through qualitative
| |
| - | observation, allowsus to reach certain conclusions. The bleeching of the color
| |
| - | primarily indicates that the enzymes were synthesized. therefore no
| |
| - | contamination took place. Apart from the synthesis factor, the conclusion
| |
| - | that the conformational structure is functional can be reached. This will
| |
| - | in turn provide insight that the other desired proteins will also be
| |
| - | synthesized. The system will also enable the ability to evaluate the efficiency
| |
| - | of the system through the gradient of color concentration.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>-The changes
| |
| - | in absorbance will ease the acquirement of the certain results. The absorbance <span
| |
| - | class=GramE>data</span> can be used to prepare a professional and highly
| |
| - | efficient scale for examination and use. The prepared scales can be used for
| |
| - | comparison between the future experiments and the current experiments. It would
| |
| - | be hard and even erronous to make judgement on strains that do not have high
| |
| - | protein synthesis but the comparison between the acquired absorbance values and
| |
| - | the scales not only removes the risk factor but also gives the
| |
| - | opportunity of making precise judgement in the absence of visual <span
| |
| - | class=GramE>data</span>.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'> </span><!--[if gte vml 1]><v:shapetype
| |
| - | id="_x0000_t75" coordsize="21600,21600" o:spt="75" o:preferrelative="t"
| |
| - | path="m@4@5l@4@11@9@11@9@5xe" filled="f" stroked="f">
| |
| - | <v:stroke joinstyle="miter"/>
| |
| - | <v:formulas>
| |
| - | <v:f eqn="if lineDrawn pixelLineWidth 0"/>
| |
| - | <v:f eqn="sum @0 1 0"/>
| |
| - | <v:f eqn="sum 0 0 @1"/>
| |
| - | <v:f eqn="prod @2 1 2"/>
| |
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| |
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| |
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| |
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| |
| - | <v:f eqn="prod @7 21600 pixelHeight"/>
| |
| - | <v:f eqn="sum @10 21600 0"/>
| |
| - | </v:formulas>
| |
| - | <v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/>
| |
| - | <o:lock v:ext="edit" aspectratio="t"/>
| |
| - | </v:shapetype><v:shape id="Resim_x0020_2" o:spid="_x0000_s1026" type="#_x0000_t75"
| |
| - | alt="https://static.igem.org/mediawiki/2014hs/e/e3/Ekran_Al%C4%B1nt%C4%B1s%C4%B1.PNG"
| |
| - | style='position:absolute;margin-left:-31.5pt;margin-top:-1945.5pt;width:582pt;
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| - | height:121.5pt;z-index:251659264;visibility:visible;mso-wrap-style:square;
| |
| - | mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt;
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| - | mso-wrap-distance-top:0;mso-wrap-distance-right:9pt;
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| - | mso-wrap-distance-bottom:0;mso-position-horizontal:absolute;
| |
| - | mso-position-horizontal-relative:text;mso-position-vertical:absolute;
| |
| - | mso-position-vertical-relative:line;mso-width-percent:0;mso-height-percent:0;
| |
| - | mso-width-relative:page;mso-height-relative:page' o:allowoverlap="f">
| |
| - | <v:imagedata src="https://static.igem.org/mediawiki/2014hs/e/e3/Ekran_Al%C4%B1nt%C4%B1s%C4%B1.PNG"/>
| |
| - | <w:wrap type="square" anchory="line"/>
| |
| - | </v:shape><![endif]--><![if !vml]><img width=776 height=162
| |
| - | src="https://static.igem.org/mediawiki/2014hs/e/e3/Ekran_Al%C4%B1nt%C4%B1s%C4%B1.PNG"
| |
| - | align=left hspace=12
| |
| - | alt="https://static.igem.org/mediawiki/2014hs/e/e3/Ekran_Al%C4%B1nt%C4%B1s%C4%B1.PNG"
| |
| - | v:shapes="Resim_x0020_2"><![endif]></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'> </span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'><o:p> </o:p></span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'><o:p> </o:p></span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'><o:p> </o:p></span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'><o:p> </o:p></span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Advanced
| |
| - | System</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>The main
| |
| - | principle of our advanced system that has brought a new dimension to reporter
| |
| - | systems lies in the variance in the activity levels of the lignin peroxidase
| |
| - | and horseradish peroxidase enzymes. The enzymes to have effect on one specific
| |
| - | dye at the same time would not bring us any experimental gain. For this reason,
| |
| - | the advanced system contains two different dyes alongside the two enzymes. The
| |
| - | system is built around the inability of one enzyme in degrading the one dye
| |
| - | while the other can, while the opposite is true for the other dye. Experimental
| |
| - | systems containing a complex unison of enzymes and dyes can be constructed.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>To further
| |
| - | enhance our project, we formed such a complex system. The bacteria were given
| |
| - | two parts, one containing horseradish peroxidase while the other contains
| |
| - | lignin peroxidase. The dyes that were selected were Methyl green and Azure-b.
| |
| - | While methyl green is, as the name implies, green colored, azure-b has a color
| |
| - | in between pink and purple. Horseradish peroxidase can effectively degrade
| |
| - | methyl green but lacks the ability to effectively degrade azure-b meanwhile
| |
| - | lignin peroxidase is active in the degradation of azure-b and lackluster in
| |
| - | degrading methyl green.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>If parts
| |
| - | starting off with different inducible promoters were transformed to the same
| |
| - | bacteria, different <span class=GramE>data</span> from different conditions
| |
| - | could be acquired. Think of a system in which the bacteria have inducible
| |
| - | promoters that are oxygen sensitive and the other carbon dioxide sensitive. If
| |
| - | methyl green and azure-b was to be added to cause interaction with the bacteria,
| |
| - | the varying levels of oxygen and carbon dioxide would therefore result in
| |
| - | different levels of enzymatic activity. Let’s say that the oxygen inducible
| |
| - | bacteria contain genes that code for horseradish peroxidase and the carbon
| |
| - | dioxide inducible bacteria contain genes that code for lignin peroxidase. <span
| |
| - | class=GramE>The variation in the level of oxygen and carbon dioxide would
| |
| - | change the rate of specific enzyme activity hence leading to a difference in
| |
| - | the degradation levels of methyl green and azure-b. This would change the color
| |
| - | of the resultant substrate thus allowing the identification of the activity of
| |
| - | the inducible promoters. </span>In this way, scales could be prepared for many
| |
| - | different types of inducible promoters for countless possibilities.</span></p>
| |
| - |
| |
| - | <div>
| |
| - |
| |
| - | <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt'><span
| |
| - | style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif";
| |
| - | mso-fareast-font-family:"Times New Roman"'><br clear=all style='mso-special-character:
| |
| - | line-break'>
| |
| - | <o:p></o:p></span></p>
| |
| - |
| |
| - | <div class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt'><span
| |
| - | style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif";
| |
| - | mso-fareast-font-family:"Times New Roman"'>
| |
| - |
| |
| - | <hr size=1 width="33%" align=left>
| |
| - |
| |
| - | </span></div>
| |
| - |
| |
| - | <div id=edn1>
| |
| - |
| |
| - | <p class=MsoEndnoteText><a name="_edn1"></a><a href="#_ednref1" title=""><span
| |
| - | style='mso-bookmark:_edn1'><span class=MsoEndnoteReference>[i]</span></span><span
| |
| - | style='mso-bookmark:_edn1'></span></a><span style='mso-bookmark:_edn1'></span> <sup><span
| |
| - | style='color:#252525'>K.E.L. Eriksson, R.A. Blanchette and P. Ander (1990).
| |
| - | "Microbial and Enzymatic Degradation of Wood and Wood Components,". <i>Springer-Verlag</i></span></sup></p>
| |
| - |
| |
| - | <p class=MsoEndnoteText><sup>[i] </sup><sup><span style='font-size:9.0pt;
| |
| - | color:#333333'>Veitch, Nigel C<span class=GramE>.,</span> <i>Horseradish peroxidase:
| |
| - | a modern view of a classic enzyme</i>, Phytochemistry, 65, <b>2004</b>,
| |
| - | 249-259, 23-May-2010</span></sup></p>
| |
| - |
| |
| - | <p class=MsoNormal style='line-height:normal'><sup>[i]</sup><sup><span
| |
| - | style='font-size:10.0pt'> Ann B. Orth, Daniel J. Royse, Ming Tien
| |
| - | “Ubiquity of Lignin-Degrading Peroxidases among Various Wood-Degrading Fungi,”
| |
| - | Applied and Environmental Microbiology (1993):4017-4023</span></sup></p>
| |
| - |
| |
| - | <h1 style='margin-bottom:5.0pt;line-height:150%'><sup><span style='mso-fareast-font-family:
| |
| - | "Times New Roman"'>[i]</span></sup><span style='mso-fareast-font-family:"Times New Roman"'><a
| |
| - | href="http://www.sciencedirect.com/science/article/pii/0168644594900760"><span
| |
| - | style='font-size:8.0pt;line-height:150%;font-family:"Arial","sans-serif";
| |
| - | color:#316C9D;text-decoration:none;text-underline:none'>Annele Hatakka</span></a></span><span
| |
| - | style='font-size:8.0pt;line-height:150%;font-family:"Arial","sans-serif";
| |
| - | mso-fareast-font-family:"Times New Roman"'>, “</span><span style='font-size:
| |
| - | 8.0pt;line-height:150%;font-family:"Arial","sans-serif";mso-fareast-font-family:
| |
| - | "Times New Roman";color:#5C5C5C'>Lignin-modifying enzymes from selected
| |
| - | white-rot fungi: production and role from in lignin degradation,” </span><span
| |
| - | style='mso-fareast-font-family:"Times New Roman"'><a
| |
| - | href="http://www.sciencedirect.com/science/journal/01686445"><span
| |
| - | style='font-size:8.0pt;line-height:150%;font-family:"Arial","sans-serif";
| |
| - | color:#2E2E2E;background:#F9FBFC;text-decoration:none;text-underline:none'>FEMS
| |
| - | Microbiology Reviews</span></a></span><span style='font-size:8.0pt;line-height:
| |
| - | 150%;font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
| |
| - | color:#5C5C5C'> (1994):125-135</span><span style='mso-fareast-font-family:"Times New Roman"'><o:p></o:p></span></h1>
| |
| - |
| |
| - | <p class=MsoNormal style='line-height:normal'><sup>[i]</sup><span
| |
| - | style='font-size:8.0pt'> <span style='color:#252525'>Renganathan V, Miki K,
| |
| - | Gold MH (1985). "Multiple molecular forms of diarylpropane oxygenase, an
| |
| - | H2O2-requiring, lignin-degrading enzyme from Phanerochaete chrysosporium".
| |
| - | <i>Arch. Biochem. Biophys.</i> <b>241</b> (1): 304–14. </span></span><a
| |
| - | href="http://en.wikipedia.org/wiki/Digital_object_identifier"><span
| |
| - | style='font-size:8.0pt;color:#0B0080;text-decoration:none;text-underline:none'>doi</span></a><span
| |
| - | style='font-size:8.0pt;color:#252525'>:</span><a
| |
| - | href="http://dx.doi.org/10.1016%2F0003-9861%2885%2990387-X"><span
| |
| - | style='font-size:8.0pt;color:#663366;text-decoration:none;text-underline:none'>10.1016/0003-9861(85)90387-X</span></a><span
| |
| - | style='font-size:8.0pt;color:#252525'>. </span><a
| |
| - | href="http://en.wikipedia.org/wiki/PubMed_Identifier"><span style='font-size:
| |
| - | 8.0pt;color:#0B0080;text-decoration:none;text-underline:none'>PMID</span></a><span
| |
| - | style='font-size:8.0pt;color:#252525'> </span><a
| |
| - | href="http://www.ncbi.nlm.nih.gov/pubmed/4026322"><span style='font-size:8.0pt;
| |
| - | color:#663366;text-decoration:none;text-underline:none'>4026322</span></a></p>
| |
| - |
| |
| - | <p class=MsoEndnoteText><span class=GramE><sup>[i]</sup><a
| |
| - | href="http://www.jbc.org/search?author1=Susana+Camarero&sortspec=date&submit=Submit"><b><span
| |
| - | style='font-size:8.0pt;color:#333333;text-decoration:none;text-underline:none'>Susana
| |
| - | Camarero</span></b></a><b><span style='font-size:8.0pt;color:#403838'>, </span></b><a
| |
| - | href="http://www.jbc.org/search?author1=Sovan+Sarkar&sortspec=date&submit=Submit"><b><span
| |
| - | style='font-size:8.0pt;color:#333333;text-decoration:none;text-underline:none'>Sovan
| |
| - | Sarkar</span></b></a><b><span style='font-size:8.0pt;color:#403838'>, </span></b><a
| |
| - | href="http://www.jbc.org/search?author1=Francisco+Javier+Ruiz-Due%C3%B1as&sortspec=date&submit=Submit"><b><span
| |
| - | style='font-size:8.0pt;color:#333333;text-decoration:none;text-underline:none'>Francisco
| |
| - | Javier Ruiz-Dueñas</span></b></a><b><span style='font-size:8.0pt;color:#403838'>,</span></b><a
| |
| - | href="http://www.jbc.org/search?author1=Mar%C4%B1%CC%81a+Jes%C3%BAs+Mart%C4%B1%CC%81nez&sortspec=date&submit=Submit"><b><span
| |
| - | style='font-size:8.0pt;color:#333333;text-decoration:none;text-underline:none'>Marı́a
| |
| - | Jesús Martı́nez</span></b></a><b><span style='font-size:8.0pt;color:#403838'>
| |
| - | and </span></b><a
| |
| - | href="http://www.jbc.org/search?author1=%C3%81ngel+T.+Mart%C4%B1%CC%81nez&sortspec=date&submit=Submit"><b><span
| |
| - | style='font-size:8.0pt;color:#333333;text-decoration:none;text-underline:none'>Ángel
| |
| - | T. Martı́nez “</span></b></a><span style='font-size:8.0pt'>Description of
| |
| - | a Versatile Peroxidase Involved in the Natural Degradation of Lignin That Has
| |
| - | Both Manganese Peroxidase and Lignin Peroxidase Substrate Interaction Sites,” <span
| |
| - | style='color:#403838'>Centro de Investigaciones Biológicas, Consejo Superior de
| |
| - | Investigaciones Cientı́ficas, Velázquez 144, E-28006 Madrid, Spain, last
| |
| - | accessed 06.21.2014, </span></span><a
| |
| - | href="http://www.jbc.org/content/274/15/10324.short"><span style='font-size:
| |
| - | 8.0pt'>http://www.jbc.org/content/274/15/10324.short</span></a></span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='line-height:normal'><sup>[i]</sup><span
| |
| - | style='font-size:10.0pt'> Jean Luc Wertz and Olivier Bedue, Lignocellulosic
| |
| - | Biorefineries (Lausanne: EPFL Press, 2013), 278-286</span></p>
| |
| - |
| |
| - | <h1 style='margin-bottom:5.0pt;line-height:150%'><sup><span style='mso-fareast-font-family:
| |
| - | "Times New Roman"'>[i]</span></sup><span style='mso-fareast-font-family:"Times New Roman"'><a
| |
| - | href="http://www.sciencedirect.com/science/article/pii/S0003986199913656"><span
| |
| - | style='font-size:10.0pt;line-height:150%;color:#316C9D;text-decoration:none;
| |
| - | text-underline:none'>Wolfgang Blodig</span></a><a
| |
| - | href="http://www.sciencedirect.com/science/article/pii/S0003986199913656#A1"><sup><span
| |
| - | style='font-size:12.0pt;line-height:150%;color:#316C9D;text-decoration:none;
| |
| - | text-underline:none'>a</span></sup></a></span><sup><span style='font-size:12.0pt;
| |
| - | line-height:150%;mso-fareast-font-family:"Times New Roman";color:#2E2E2E'>, </span></sup><span
| |
| - | style='mso-fareast-font-family:"Times New Roman"'><a
| |
| - | href="http://www.sciencedirect.com/science/article/pii/S0003986199913656#FN1"><sup><span
| |
| - | style='font-size:12.0pt;line-height:150%;color:#316C9D;text-decoration:none;
| |
| - | text-underline:none'>1</span></sup></a></span><span style='font-size:10.0pt;
| |
| - | line-height:150%;mso-fareast-font-family:"Times New Roman";color:#2E2E2E'>, </span><span
| |
| - | style='mso-fareast-font-family:"Times New Roman"'><a
| |
| - | href="http://www.sciencedirect.com/science/article/pii/S0003986199913656"><span
| |
| - | style='font-size:10.0pt;line-height:150%;color:#316C9D;text-decoration:none;
| |
| - | text-underline:none'>Andrew T. Smith</span></a><a
| |
| - | href="http://www.sciencedirect.com/science/article/pii/S0003986199913656#A2"><sup><span
| |
| - | style='font-size:12.0pt;line-height:150%;color:#316C9D;text-decoration:none;
| |
| - | text-underline:none'>b</span></sup></a></span><span style='font-size:10.0pt;
| |
| - | line-height:150%;mso-fareast-font-family:"Times New Roman";color:#2E2E2E'>, </span><span
| |
| - | style='mso-fareast-font-family:"Times New Roman"'><a
| |
| - | href="http://www.sciencedirect.com/science/article/pii/S0003986199913656"><span
| |
| - | style='font-size:10.0pt;line-height:150%;color:#316C9D;text-decoration:none;
| |
| - | text-underline:none'>Kaspar Winterhalter</span></a><a
| |
| - | href="http://www.sciencedirect.com/science/article/pii/S0003986199913656#A1"><sup><span
| |
| - | style='font-size:12.0pt;line-height:150%;color:#316C9D;text-decoration:none;
| |
| - | text-underline:none'>a</span></sup></a></span><span style='font-size:10.0pt;
| |
| - | line-height:150%;mso-fareast-font-family:"Times New Roman";color:#2E2E2E'>, </span><span
| |
| - | style='mso-fareast-font-family:"Times New Roman"'><a
| |
| - | href="http://www.sciencedirect.com/science/article/pii/S0003986199913656"><span
| |
| - | style='font-size:10.0pt;line-height:150%;color:#316C9D;text-decoration:none;
| |
| - | text-underline:none'>Klaus Piontek “</span></a><a
| |
| - | href="http://www.sciencedirect.com/science/article/pii/S0003986199913656"><b><span
| |
| - | style='font-size:8.0pt;line-height:150%;font-family:"Arial","sans-serif";
| |
| - | color:#5C5C5C;text-decoration:none;text-underline:none'>Evidence from
| |
| - | Spin-Trapping for a Transient Radical on Tryptophan Residue 171 of Lignin
| |
| - | Peroxidase,” </span></b></a><a
| |
| - | href="http://www.sciencedirect.com/science/journal/00039861"><b><span
| |
| - | style='font-size:8.0pt;line-height:150%;font-family:"Arial","sans-serif";
| |
| - | color:#2E2E2E;background:#F9FBFC'>Archives of Biochemistry and Biophysic</span></b></a></span><span
| |
| - | style='font-size:8.0pt;line-height:150%;mso-fareast-font-family:"Times New Roman"'>s</span><b><span
| |
| - | style='font-size:8.0pt;line-height:150%;font-family:"Arial","sans-serif";
| |
| - | mso-fareast-font-family:"Times New Roman"'> (1999):86-92</span></b><span
| |
| - | style='mso-fareast-font-family:"Times New Roman"'><o:p></o:p></span></h1>
| |
| - |
| |
| - | <h1 style='margin-top:5.0pt;margin-right:0cm;margin-bottom:11.0pt;margin-left:
| |
| - | 0cm;line-height:120%'><sup><span style='mso-fareast-font-family:"Times New Roman"'>[i]</span></sup><span
| |
| - | style='font-size:8.0pt;line-height:120%;mso-fareast-font-family:"Times New Roman"'>
| |
| - | </span><span style='mso-fareast-font-family:"Times New Roman"'><a
| |
| - | href="http://link.springer.com/search?facet-author=%22M.+Yadav%22"><span
| |
| - | style='font-size:8.0pt;line-height:120%;color:#333333;text-decoration:none;
| |
| - | text-underline:none'>M. Yadav</span></a></span><span style='font-size:8.0pt;
| |
| - | line-height:120%;mso-fareast-font-family:"Times New Roman";color:#333333'>, </span><span
| |
| - | style='mso-fareast-font-family:"Times New Roman"'><a
| |
| - | href="http://link.springer.com/search?facet-author=%22P.+Yadav%22"><span
| |
| - | style='font-size:8.0pt;line-height:120%;color:#333333;text-decoration:none;
| |
| - | text-underline:none'>P. Yadav</span></a></span><span style='font-size:8.0pt;
| |
| - | line-height:120%;mso-fareast-font-family:"Times New Roman";color:#333333'>, </span><span
| |
| - | style='mso-fareast-font-family:"Times New Roman"'><a
| |
| - | href="http://link.springer.com/search?facet-author=%22K.+D.+S.+Yadav%22"><span
| |
| - | style='font-size:8.0pt;line-height:120%;color:#333333;text-decoration:none;
| |
| - | text-underline:none'>K. D. S. Yadav</span></a></span><span style='font-size:
| |
| - | 8.0pt;line-height:120%;mso-fareast-font-family:"Times New Roman"'>. “</span><span
| |
| - | style='font-size:8.0pt;line-height:120%;font-family:"Georgia","serif";
| |
| - | mso-fareast-font-family:"Times New Roman";color:#333333'>Purification,
| |
| - | characterization, and coal depolymerizing activity of lignin peroxidase from <i>Gloeophyllum
| |
| - | sepiarium</i>,” </span><span style='mso-fareast-font-family:"Times New Roman"'><a
| |
| - | href="http://link.springer.com/journal/10541"><span style='font-size:8.0pt;
| |
| - | line-height:120%;font-family:"Arial","sans-serif";color:#333333;text-decoration:
| |
| - | none;text-underline:none'>Biochemistry (Moscow)</span></a></span><span
| |
| - | style='font-size:8.0pt;line-height:120%;mso-fareast-font-family:"Times New Roman"'>
| |
| - | (2009):1125-1131</span><span style='mso-fareast-font-family:"Times New Roman"'><o:p></o:p></span></h1>
| |
| - |
| |
| - | <p class=MsoNormal style='line-height:normal'><sup>[i]</sup><sup><span
| |
| - | style='font-size:10.0pt'> <span style='color:#252525'> </span></span></sup><a
| |
| - | href="http://en.wikipedia.org/wiki/Protein_Data_Bank"><sup><span
| |
| - | style='font-size:10.0pt;color:#0B0080;text-decoration:none;text-underline:none'>PDB</span></sup></a><sup><span
| |
| - | style='font-size:10.0pt;color:#252525'> </span></sup><a
| |
| - | href="http://www.rcsb.org/pdb/explore/explore.do?structureId=1GWU"><sup><span
| |
| - | style='font-size:10.0pt;color:#663366;text-decoration:none;text-underline:none'>1GWU</span></sup></a><sup><span
| |
| - | style='font-size:10.0pt;color:#252525'>; Gajhede M, Schuller DJ, Henriksen A,
| |
| - | Smith AT, Poulos TL (December 1997). "Crystal structure of horseradish
| |
| - | peroxidase C at 2.15 A resolution". <i>Nat. Struct. Biol.</i> <b>4</b>(12):
| |
| - | 1032–8. </span></sup><a
| |
| - | href="http://en.wikipedia.org/wiki/Digital_object_identifier"><sup><span
| |
| - | style='font-size:10.0pt;color:#0B0080;text-decoration:none;text-underline:none'>doi</span></sup></a><sup><span
| |
| - | style='font-size:10.0pt;color:#252525'>:</span></sup><a
| |
| - | href="http://dx.doi.org/10.1038%2Fnsb1297-1032"><span class=GramE><sup><span
| |
| - | style='font-size:10.0pt;color:#663366;text-decoration:none;text-underline:none'>10.1038</span></sup></span><sup><span
| |
| - | style='font-size:10.0pt;color:#663366;text-decoration:none;text-underline:none'>/nsb1297-1032</span></sup></a><sup><span
| |
| - | style='font-size:10.0pt;color:#252525'>. </span></sup><a
| |
| - | href="http://en.wikipedia.org/wiki/PubMed_Identifier"><sup><span
| |
| - | style='font-size:10.0pt;color:#0B0080;text-decoration:none;text-underline:none'>PMID</span></sup></a><sup><span
| |
| - | style='font-size:10.0pt;color:#252525'> </span></sup><a
| |
| - | href="http://www.ncbi.nlm.nih.gov/pubmed/9406554"><sup><span style='font-size:
| |
| - | 10.0pt;color:#663366;text-decoration:none;text-underline:none'>9406554</span></sup></a></p>
| |
| - |
| |
| - | <p class=MsoEndnoteText><sup>[i] <span style='color:#252525'> </span></sup><a
| |
| - | href="http://www.ebi.ac.uk/pdbe-apps/widgets/unipdb?uniprot=P00433"><sup><span
| |
| - | style='color:#663366;text-decoration:none;text-underline:none'>"Peroxidase
| |
| - | C1A Related PDB sequences"</span></sup></a><sup><span style='color:#252525'>.
| |
| - | <i>UniPDB</i>. European Bioinformatics Institute</span></sup></p>
| |
| - |
| |
| - | <p class=MsoEndnoteText><sup>[i] <span style='color:#252525'>Veitch NC
| |
| - | (February 2004). "Horseradish peroxidase: a modern view of a classic
| |
| - | enzyme". </span></sup><a
| |
| - | href="http://en.wikipedia.org/wiki/Phytochemistry_(journal)"><i><sup><span
| |
| - | style='color:#0B0080;text-decoration:none;text-underline:none'>Phytochemistry</span></sup></i></a><sup><span
| |
| - | style='color:#252525'> <b>65</b> (3): 249–59. </span></sup><a
| |
| - | href="http://en.wikipedia.org/wiki/Digital_object_identifier"><sup><span
| |
| - | style='color:#0B0080;text-decoration:none;text-underline:none'>doi</span></sup></a><sup><span
| |
| - | style='color:#252525'>:</span></sup><a
| |
| - | href="http://dx.doi.org/10.1016%2Fj.phytochem.2003.10.022"><span class=GramE><sup><span
| |
| - | style='color:#663366;text-decoration:none;text-underline:none'>10.1016</span></sup></span><sup><span
| |
| - | style='color:#663366;text-decoration:none;text-underline:none'>/j.phytochem.2003.10.022</span></sup></a><sup><span
| |
| - | style='color:#252525'>. </span></sup><a
| |
| - | href="http://en.wikipedia.org/wiki/PubMed_Identifier"><sup><span
| |
| - | style='color:#0B0080;text-decoration:none;text-underline:none'>PMID</span></sup></a><sup><span
| |
| - | style='color:#252525'> </span></sup><a
| |
| - | href="http://www.ncbi.nlm.nih.gov/pubmed/14751298"><span class=GramE><sup><span
| |
| - | style='color:#663366;text-decoration:none;text-underline:none'>14751298</span></sup></span></a></p>
| |
| - |
| |
| - | <p class=MsoEndnoteText> </p>
| |
| - |
| |
| - | <p class=MsoNormal style='line-height:normal'><sup>[i]</sup><sup><span
| |
| - | style='font-size:10.0pt'> <span style='color:#252525'>Akkara JA, Senecal KJ,
| |
| - | Kaplan DL (October 1991). "Synthesis and characterization of polymers
| |
| - | produced by horseradish peroxidase in dioxane". <i>Journal of Polymer
| |
| - | Science</i> <b>29</b> (11): 1561–74. </span></span></sup><a
| |
| - | href="http://en.wikipedia.org/wiki/Digital_object_identifier"><sup><span
| |
| - | style='font-size:10.0pt;color:#0B0080;text-decoration:none;text-underline:none'>doi</span></sup></a><sup><span
| |
| - | style='font-size:10.0pt;color:#252525'>:</span></sup><a
| |
| - | href="http://dx.doi.org/10.1002%2Fpola.1991.080291105"><span class=GramE><sup><span
| |
| - | style='font-size:10.0pt;color:#663366;text-decoration:none;text-underline:none'>10.1002</span></sup></span><sup><span
| |
| - | style='font-size:10.0pt;color:#663366;text-decoration:none;text-underline:none'>/pola.1991.080291105</span></sup></a></p>
| |
| - |
| |
| - | <p class=MsoNormal style='line-height:normal'><sup>[i]</sup><sup><span
| |
| - | style='font-size:10.0pt'> <span style='color:#252525'>Chau YP, Lu KS (1995).
| |
| - | "Investigation of the blood-ganglion barrier properties in rat sympathetic
| |
| - | ganglia by using lanthanum ion and horseradish peroxidase as tracers". <i>Acta
| |
| - | Anat (Basel)</i> <b>153</b> (2): 135–44. </span></span></sup><a
| |
| - | href="http://en.wikipedia.org/wiki/Digital_object_identifier"><sup><span
| |
| - | style='font-size:10.0pt;color:#0B0080;text-decoration:none;text-underline:none'>doi</span></sup></a><sup><span
| |
| - | style='font-size:10.0pt;color:#252525'>:</span></sup><a
| |
| - | href="http://dx.doi.org/10.1159%2F000313647"><sup><span style='font-size:10.0pt;
| |
| - | color:#663366;text-decoration:none;text-underline:none'>10.1159/000313647</span></sup></a><sup><span
| |
| - | style='font-size:10.0pt;color:#252525'>. </span></sup><a
| |
| - | href="http://en.wikipedia.org/wiki/PubMed_Identifier"><sup><span
| |
| - | style='font-size:10.0pt;color:#0B0080;text-decoration:none;text-underline:none'>PMID</span></sup></a><sup><span
| |
| - | style='font-size:10.0pt;color:#252525'> </span></sup><a
| |
| - | href="http://www.ncbi.nlm.nih.gov/pubmed/8560966"><sup><span style='font-size:
| |
| - | 10.0pt;color:#663366;text-decoration:none;text-underline:none'>8560966</span></sup></a></p>
| |
| - |
| |
| - | <p class=MsoNormal style='line-height:normal'><sup>[i]</sup><sup><span
| |
| - | style='font-size:10.0pt'> <span style='color:#252525'>Lichtman JW, Purves D
| |
| - | (1985). </span></span></sup><a
| |
| - | href="http://books.google.com/books?id=t9JqAAAAMAAJ&q=In+recent+years+the+technique+of+marking+neurons+with+the+enzyme+horseradish+peroxidase+has+become+a+major+tool&dq=In+recent+years+the+technique+of+marking+neurons+with+the+enzyme+horseradish+peroxidase+has+become+a+major+tool&hl=en&sa=X&ei=-rL_T67zC6ni4QSKndWrCA&redir_esc=y"><sup><span
| |
| - | style='font-size:10.0pt;color:#663366;text-decoration:none;text-underline:none'>"Cell
| |
| - | marking with horseradish peroxidase"</span></sup></a><sup><span
| |
| - | style='font-size:10.0pt;color:#252525'>. <i>Principles of neural development</i>.
| |
| - | Sunderland, Mass: Sinauer Associates. <span class=GramE>p</span>. 114. </span></sup><a
| |
| - | href="http://en.wikipedia.org/wiki/International_Standard_Book_Number"><sup><span
| |
| - | style='font-size:10.0pt;color:#0B0080;text-decoration:none;text-underline:none'>ISBN</span></sup></a><sup><span
| |
| - | style='font-size:10.0pt;color:#252525'> </span></sup><a
| |
| - | href="http://en.wikipedia.org/wiki/Special:BookSources/0-87893-744-7"><sup><span
| |
| - | style='font-size:10.0pt;color:#0B0080;text-decoration:none;text-underline:none'>0-87893-744-7</span></sup></a></p>
| |
| - |
| |
| - | <h1 style='margin-bottom:2.0pt;line-height:80%'><sup><span style='mso-fareast-font-family:
| |
| - | "Times New Roman"'>[i]</span></sup><sup><span style='font-size:10.0pt;
| |
| - | line-height:80%;mso-fareast-font-family:"Times New Roman"'> “</span></sup><sup><span
| |
| - | style='font-size:10.0pt;line-height:80%;font-family:"Arial","sans-serif";
| |
| - | mso-fareast-font-family:"Times New Roman";color:#282828'>Part:BBa_J23100,” last
| |
| - | accessed June 21, 2014, http://parts.igem.org/Part:BBa_J23100</span></sup><span
| |
| - | style='mso-fareast-font-family:"Times New Roman"'><o:p></o:p></span></h1>
| |
| - |
| |
| - | <p class=MsoNormal style='line-height:normal'><sup>[i]</sup><sup><span
| |
| - | style='font-size:10.0pt'> J.K. Spiker, D.L. Crawford, E.C. Thiel, Appl.
| |
| - | Environ.</span></sup></p>
| |
| - |
| |
| - | <p class=MsoNormal style='line-height:normal'><sup>[i]</sup><sup><span
| |
| - | style='font-size:10.0pt'>Microbiol. 37 (1992) 518–523.</span></sup></p>
| |
| - |
| |
| - | <p class=MsoNormal style='line-height:normal'><sup>[i]</sup><sup><span
| |
| - | style='font-size:10.0pt'> M. Tien, T.K. Kirk, Proc. Natl. Acad. Sci. U.S.A. 8
| |
| - | (1984)</span></sup></p>
| |
| - |
| |
| - | <p class=MsoEndnoteText> </p>
| |
| - |
| |
| - | <p class=MsoEndnoteText> </p>
| |
| - |
| |
| - | </div>
| |
| - |
| |
| - | </div>
| |
| - |
| |
| - | </div>
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| - |
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| - | </body>
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| - |
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| - | </head>
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| - | </html>
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