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Revision as of 03:09, 21 June 2014

• Home
• Our City
  • Outreach
• Project
  • Design
  • Procedure
  • Results of Gel 1
  • Results of Gel 2
  • Results of Initial Subtilisin Test
  • Results of Complete Subtilisin Test
  • Human Practices
  • Safety
• E-Notebook
• Team
• Our Poster
• Sponsors

Restriction Digest of DNA from Distribution Kit:

Lanes left to right:

1. Ladder 2. DNA cut with EcoR1 3. DNA cut with Spe1 4. DNA cut with Xba1 5. DNA cut with Pst1

This picture shows our gel of the restriction digest of DNA from the DNA distribution kit. If the restriction enzymes were working, EcoR1, Spe1, Xba1, and Pst1 should cut the DNA at their designated restriction sites and consequently DNA fragments should appear on the gel. The results of the gel show that the DNA was never digested, and therefore the restriction enzymes may not have been functional or we had an error in our procedure.