"
Team:FHS Frederick MD/Notebook
From 2014hs.igem.org
(Difference between revisions)
(Added footer) |
(→Lab Notebook: Notebook photo) |
||
| Line 2: | Line 2: | ||
=Lab Notebook= | =Lab Notebook= | ||
| - | + | [[File:FHS_Notes.jpg|right|300px]] | |
* [http://arsbiotechnica.org/w/Article:002-001 20 Feb 2014]: Received iGEM supplies | * [http://arsbiotechnica.org/w/Article:002-001 20 Feb 2014]: Received iGEM supplies | ||
* [http://arsbiotechnica.org/w/Record:002-010 03 Apr 2014]: Digested NirB and LOV inserts | * [http://arsbiotechnica.org/w/Record:002-010 03 Apr 2014]: Digested NirB and LOV inserts | ||
Latest revision as of 02:45, 21 June 2014
Lab Notebook
- [http://arsbiotechnica.org/w/Article:002-001 20 Feb 2014]: Received iGEM supplies
- [http://arsbiotechnica.org/w/Record:002-010 03 Apr 2014]: Digested NirB and LOV inserts
- [http://arsbiotechnica.org/w/Record:002-011 10 Apr 2014]: Ligated NirB and LOV genes into pSB1C3
- [http://arsbiotechnica.org/w/Record:002-012 14 Apr 2014]: Transformed E. coli with LOV and NirB constructs
- [http://arsbiotechnica.org/w/Record:002-013 21 Apr 2014]: Ligated NirB and LOV genes into pSB1C3
- [http://arsbiotechnica.org/w/Record:002-014 28 Apr 2014]: Transformed E. coli with LOV and NirB constructs
- [http://arsbiotechnica.org/w/Record:002-015 01 May 2014]: Prepared TSS buffer
- [http://arsbiotechnica.org/w/Record:002-016 05 May 2014]: Ligatied the RFP plasmid
- [http://arsbiotechnica.org/w/Record:002-017 08 May 2014]: Subcultured E. coli NEB10 Beta
- [http://arsbiotechnica.org/w/Record:002-018 08 May 2014]: Tested transformation efficiency of competent cells
- [http://arsbiotechnica.org/w/Record:002-020 12 May 2014]: Digested NirB, LOV, pSB1C3 DNA fragments with EcoRI and PstI
- [http://arsbiotechnica.org/w/Record:002-021 15 May 2014]: Ligated NirB and LOV into pSB1C3
- [http://arsbiotechnica.org/w/Record:002-022 20 May 2014]: Transformed E. coli with LOV and NirB constructs
- [http://arsbiotechnica.org/w/Record:002-023 29 May 2014]: Cultured individual NirB and LOV colonies
- [http://arsbiotechnica.org/w/Record:002-024 02 Jun 2014]: Purified NirB and LOV plasmids
- [http://arsbiotechnica.org/w/Record:002-025 03 Jun 2014]: Visualized NirB and LOV plasmids on a gel
- [http://arsbiotechnica.org/w/Record:002-026 04 Jun 2014]: Recultured NirB and LOV transformants
- [http://arsbiotechnica.org/w/Record:002-027 05 Jun 2014]: Extracted plasmid DNA from bacterial transformants
- [http://arsbiotechnica.org/w/Record:002-028 06 Jun 2014]: Observed plasmid DNA on a gel
- [http://arsbiotechnica.org/w/Record:002-029 09 Jun 2014]: Submitted samples for sequencing
- [http://arsbiotechnica.org/w/Record:002-030 11 Jun 2014]: Resubmitted DNA for sequencing
