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Would any of your project ideas raise safety issues in terms of: researcher safety, public safety, or environmental safety?
Q2:Do any of the new BioBrick parts (or devices) that you made this year raise safety issues?
Q3:Is there a local biosafety group, committee, or review board at your institution?
Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?
Answer2: No, all new BioBrick parts, including CRISPR-Cas9 enzyme, guide-RNA and homologous arms, do not raised safety issues even under the case of accidental leakage. The enzyme will not pose danger by editing genes of any species unless are maliciously designed.
Answer3: Our high school, Nanjing Foreign Language School, does not have its biosafety committee or group. However, it has strict regulations regarding biosafety and laboratory safety, which are accordingly followed by all members participating this iGEM project. The China Pharmaceutical University, in which the laboratory experiments of this project is carried out, has its special office responsible for biosafety inspection. It is well aware of the ongoing of our project and has approved all usage of bio materials and equipment. We also follow the National Accreditation Criteria for Laboratory Bio-safety.
The gene editing tool kit that we designed can be applied into the deletion and knock-out of stably integrated gene system. From a biosafety perspective, this tool kit can be used to remove genetic engineering research and development projects to filter the use of antibiotic resistance genes, thereby preventing the gene spread in subsequent research and applications, improve the safety of resistance genes used.
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