Team:FHS Frederick MD/NirB Promoter

From 2014hs.igem.org

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=NirB Promoter=
=NirB Promoter=
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The NirB gene is reliant on a fumarate and nitrate reductase (FNR), which allows the promoter to activate when there is no oxygen present, as well as facilitates in the regulation of transcription that is responsible for the growth under anaerobic conditions. When oxygen is not present, the 4Fe-4S complex helps join the FNR components. As this happens, it becomes a protein that attaches to the NirB part of the DNA, which results in the production of the LOV gene.  
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The NirB gene is reliant on a fumarate and nitrate reductase (FNR), which allows the promoter to activate when there is no oxygen present, as well as facilitate regulation of transcription that is responsible for the growth under anaerobic conditions. When oxygen is not present, the 4Fe-4S complex helps join two FNR components. As this happens, it becomes a protein complex that attaches to the NirB promoter part of the DNA, which results in the production of the [[Team:FHS_Frederick_MD/LOV_Domain|LOV protein]].  
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In an experiment by Morales, sterile oil was glossed over the tube containing the bacteria. The bacteria were then able to use the oxygen until the levels were completely depleted, resulting in a fully anaerobic environment.  Once fully anaerobic, the bacteria continued to produce the LOV gene product.
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In an experiment by the 2012 Valencia Biocampus iGEM team (see [http://parts.igem.org/Part:BBa_K763002 BBa_K763002]), sterile oil was glossed over the tube containing the bacteria. The bacteria were then able to use the oxygen until the levels were completely depleted, resulting in a fully anaerobic environment.  Once fully anaerobic, the bacteria began to produce the team's fluorescent gene product, which was under the control of the NirB promoter.
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Morales, Pedro Luis Dorado. "pNirB + Gene encoding ZsGreen1." . http://parts.igem.org/Part:BBa_K763002 (accessed June 16, 2014).
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We will similarly use the oxygen-sensitive NirB promoter to regulate transcription of the fluorescent [[Team:FHS_Frederick_MD/LOV_Domain|LOV protein]] that we are designing.

Revision as of 13:28, 20 June 2014

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NirB Promoter

The NirB gene is reliant on a fumarate and nitrate reductase (FNR), which allows the promoter to activate when there is no oxygen present, as well as facilitate regulation of transcription that is responsible for the growth under anaerobic conditions. When oxygen is not present, the 4Fe-4S complex helps join two FNR components. As this happens, it becomes a protein complex that attaches to the NirB promoter part of the DNA, which results in the production of the LOV protein.

In an experiment by the 2012 Valencia Biocampus iGEM team (see [http://parts.igem.org/Part:BBa_K763002 BBa_K763002]), sterile oil was glossed over the tube containing the bacteria. The bacteria were then able to use the oxygen until the levels were completely depleted, resulting in a fully anaerobic environment. Once fully anaerobic, the bacteria began to produce the team's fluorescent gene product, which was under the control of the NirB promoter.

We will similarly use the oxygen-sensitive NirB promoter to regulate transcription of the fluorescent LOV protein that we are designing.
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