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Team:NGSS TR/catechol.html
From 2014hs.igem.org
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</section> | </section> | ||
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| + | <blockquote> | ||
| + | <h1>PROTOCOLS</h1> | ||
| + | <blockquote> | ||
| + | <h2><span class="gfdg">2.CATECHOL PROTOCOL</span></h2> | ||
| + | <p><br /> | ||
| + | Catechol assay is performed in the plate reader on a 96 well plate<br /> | ||
| + | Each well must be filled with 100um of solution<br /> | ||
| + | Usually use 90ul of cell culture and 10um of catechol solution<br /> | ||
| + | Catechol stock solution is at 100mM concentration. And when added to the well we have a 10fold dilution. For example if an aliquot concentration of 1mM catechol is made, which would be used for assay, when the well is added catechol drops to a concentration of 0.1mM.<br /> | ||
| + | Always dilute catechol with H2O.<br /> | ||
| + | Always have a blank of 90ul medium (the one which you grew the cells overnight) with 10ul catechol solution<br /> | ||
| + | Always have a negative of 90ul growing cells and 10ul of H2O.</p> | ||
| + | <p>Reference: https://2010.igem.org/Team:Imperial_College_London</p> | ||
| + | </blockquote> | ||
| + | </blockquote> | ||
| + | <blockquote> | ||
Latest revision as of 11:20, 20 June 2014
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