Team:CIDEB-UANL Mexico/project resistance

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Description Capture Module Aroma Module Resistance Module Union Module Parts

iGEM CIDEB 2014 - Project

Resistance Module

Because our project is a biofilter which allows to remove salt from water, the bacteria needs to survive to extreme environmental conditions which normally it can’t do. We need E. coli to survive a high salinity environment to allow it to capture sodium ions and to remove the salt concentration of the water.

In order to accomplish our project goal, we have to change E. coli metabolism and make it stronger, more resistant and more efficient than in normal E. coli bacteria.

Last discoveries show irrE as a protein capable of change the E. coli metabolism, and giving it the ability to survive to bigger temperatures, bigger UV rays radiation and bigger salt concentration (UCL, 2012).

What does irrE do?

The protein irrE originates from Deinococcus radiodurans, and initially, this gene provides resistance to radiation. But when transformed in E. coli, it protects it against salt, oxidative and thermal shock. (UCL, 2012) Also, different experiments from different iGEM teams, support the idea of the bigger salt resistance in E. coli with this biobrick.

Image 1. This graph done by UCL iGEM 2012, shows how irrE biobrick increased the salt concentration resistance in E. coli compared with the results from Tu Delf iGEM 2010.

How it works?

irrE has been demonstrated to up regulate transcription of recA and pprA - genes which encode Recombines A and Radiation Inducible Protein. With respect to salt tolerance, irrE up regulates the production of several stress responsive proteins, protein kinases, metabolic proteins, and detoxification proteins. It also down regulates glycerol degradation. With this global regulatory effect, E. coli becomes more salt tolerant (UCL, 2012).

Image 2. This diagram shows the effect of irrE protein on E. coli metabolism.

How it is composed?

Initially, IrrE and L2+AIDA, protein for binding silica (Union module), were joined together in only one circuit, but we needed to separate them becauseL2+AIDA has not been proved yet and it could affect the correct production of IrrE (see image 3).

Image 3. Circuit for our project and for testing resistance and union modules.

Other teams that used it:

UCL 2012: They propose to confer salt tolerance on E. coli by linking the salt tolerance gene encoding the protein irrE (BBa_K729001) to a constitutive promoter (BBa_J23119).

Image 3. Part designed by UCL 2012 for the irrE protein.

irrE's parts description :

IMAGE	CODE	DESCRIPTION
	BBa_J23119	In the specific case of our bacteria, it helps to continuously transcribing the irrE gene in order to make bacteria resist high concentration of salt. This promoter has a length of 35 pb(Anderson, 2006).
	BBa_B0034	This specific is RBS based on Elowitz repressilator. It is very common to see in many iGEM projects. It has a length of 12 pb (Mahajan, Marinescu, Chow, Wissner-Gross, & Carr, 2003).
	BBa_K729001	Gene that produces irrE, a substance that changes the bacteria’s metabolism and allows bacteria to survive to extreme conditions, some examples could be high UV rays exposition, or high salt concentration levels in an aquatic environment, oxidative or thermal shock. It has a length of 933pb (Sohrabi, 2012).
	BBa_B1002	Part made of 6pb responsible for transcription stop (Huang, 206).

Project Zoom In

Bibliography/References

● Antiquity 2013. (2013, January 31). Part:BBa_B0034. Retrieved from http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034.

● Huang, H. (2006, August 30). Part:BBa_B1002. Retrieved August 30, 2014, from http://parts.igem.org/wiki/index.php?title=Part:BBa_B1002.

● iGEM2006_Berkeley. (2006, August 24). Part:BBa_J23119. Retrieved April 30, 2014, from http://parts.igem.org/wiki/index.php?title=Part:BBa_J23119.

● Sohrabi, B. (2012, June 27). Part:BBa_K729001. Retrieved from http://parts.igem.org/wiki/index.php?title=Part:BBa_K729001.

● UCLiGEM Team. (2012). IRRE module. Retrieved March 31, 2014. from https://2012.igem.org/Team:University_College_London/Module_5.

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iGEM CIDEB UANL 2014. CENTRO DE INVESTIGACIÓN Y DESARROLLO DE EDUCACIÓN BILINGÜE. UANL

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-	{{:Team:CIDEB-UANL_Mexico/style2.css}}{{:Team:CIDEB-UANL_Mexico/menu_project}}<html xmlns="http://www.w3.org/1999/xhtml"><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><link href='http://fonts.googleapis.com/css?family=Oxygen:400,700' rel='stylesheet' type='text/css'><title>iGEM CIDEB 2014 - Project</title><style>body{margin: 0px; width: 100%;padding: 0px;background: #2056ac;font-family: 'Oxygen', sans-serif;font-size: 12pt;background-image:url(https://static.igem.org/mediawiki/2014hs/3/32/Cideb_Fondo1.gif);}h1, h2, h3{margin: 0;padding-bottom: 5px;color: #404040;}p, ol, ul{margin-top: 0;}ol, ul{padding: 0;list-style: none;}p{line-height: 1.60em;padding- right: 3em;}strong{}a{color: #2056ac;}a:hover{text-decoration: none;}.container{margin: 0px auto;width: 1200px;}.container-text{margin: 0px auto;width: 75%;padding: 0px;font-family: 'Oxygen', sans-serif;font-size: 12pt; text-align: justify;}.wrapper{overflow: hidden;padding: 0em 0em 1em 0em;background: #FFF;}#wrapper1{background: #FFF;}#wrapper2{overflow: hidden;background: #F3F3F3;padding: 5em 0em;text-align: center;}#wrapper3{overflow: hidden;padding: 0em 0em 0em 0em;background: #FFF;}#wrapper4{}#banner{padding-top: 2em;}#welcome{overflow: hidden;width: 1000px;padding: 0em 100px 0em 100px;text-align: center;}#welcome .content{padding: 0em 8em;}#welcome .title h2{}#welcome a,#welcome strong{}.title{margin-bottom: 1em;}.title h2{font-size: 2em;}.title .byline{font-size: 1.1em;color: #6F6F6F#;}#three-column{overflow: hidden;margin-top: 5em;padding-top: 1em;border-top: 1px solid rgba(0,0,0,0.2);text-align: center;}#three-column h2{margin: 1em 0em;font-size: 1.5em;font-weight: 700;}#three-column .icon{position: relative;display: block;margin: 0px auto 0.80em auto;background: none;line-height: 150px;font-size: 4em;width: 150px;height: 100px;border-radius: 100px;border: 6px solid #67128F;text-align: center;color: #FFF;}#three-column #tbox1,#three-column #tbox2,#three-column #tbox3{float: left;width: 320px;padding: 30px 40px 50px 40px;}#three-column .title{text-align: center;}#three-column .title h2{font-size: 1.60em;}#three-column .title .byline{padding-top: 0.50em;font-size: 0.90em;color: #858585;}#three-column .arrow-down{border-top-color: #292929;}ul.tools{margin: 0;padding: 0em 0em 0em 0em;list-style: none;}ul.tools li{display: inline-block;padding: 0em .2em;font-size: 4em;}ul.tools li span{display: none;margin: 0;padding: 0;}ul.tools li a{color: #FFF;}ul.tools li a:before{display: inline-block;background: #1ABC9C;width: 120px;height: 120px;border-radius: 50%;line-height: 120px;text-align: center;color: #FFFFFF;}.button{display: inline-block;margin-top: 2em;padding: 0.8em 2em;background: #64ABD1;line-height: 1.8em;letter-spacing: 1px;text-decoration: none;font-size: 1em;color: #FFF;}.button:before{display: inline-block;background: #8DCB89;margin-right: 1em;width: 40px;height: 40px;line-height: 40px;border-radius: 20px;text-align: center;color: #272925;}.button-small{}#portfolio{overflow: hidden;padding-top: 5em;border-top: 1px solid rgba(0,0,0,0.2);}#portfolio .box{text-align: center;color: rgba(0,0,0,0.5);}#portfolio h3{display: block;padding-bottom: 1em;font-size: 1em;color: rgba(0,0,0,0.6);}#portfolio .title{text-align: center;}#portfolio .title h2{color: rgba(0,0,0,0.8);}.column1,.column2,.column3,.column4{width: 282px;}.column1,.column2,.column3{float: left;margin-right: 24px;}.column4{float: right;}</style><body><div class="wrapper"><div id="welcome" class="container"> <div class="title"> <h2>Resistance Module</h2> </div></div><div class="container-text"><table width=100%><tr><td><p>Because our project is a biofilter which allows to remove salt from water, the bacteria needs to survive to extreme environmental conditions which normally it can’t do. We need <i>E. coli</i> to survive a high salinity environment to allow it to capture sodium ions and to remove the salt concentration of the water.</p><p>In order to accomplish our project goal, we have to change <i>E. coli</i> metabolism and make it stronger, more resistant and more efficient than in normal <i>E. coli</i> bacteria.</p></td><td style="padding-left:12px;"><img width=131 height=140 src="https://static.igem.org/mediawiki/2014hs/8/8e/BeutifulResistance.jpg"/></td></tr></table><p>Last discoveries show irrE as a protein capable of change the <i>E. coli</i> metabolism, and giving it the ability to survive to bigger temperatures, bigger UV rays radiation and bigger salt concentration (UCL, 2012).</p><br><p><b>What does irrE do?</b></p><p>The protein irrE originates from <i>Deinococcusradiodurans</i>, and initially, this gene provides resistance to radiation. But when transformed in <i>E. coli</i>, it protects it against salt, oxidative and thermal shock. (UCL, 2012) Also, different experiments from different iGEM teams, support the idea of the bigger salt resistance in <i>E. coli</i> with this biobrick. </p><center><p><img width=564 height=350 src="https://static.igem.org/mediawiki/2014hs/3/3e/Grafica_1_cideb2014.png"align=center hspace=12 alt="IMG_0317"></p><p><b>Image 1.</b> This graph done by <a herf="https://2012.igem.org/Team:University_College_London">UCL iGEM 2012</a>, shows how irrE biobrick increased the salt concentration resistance in <i>E. coli</i> compared with the results from <a href="https://2010.igem.org/Team:TU_Delft">Tu Delf iGEM 2010</a>.</p></center><br><p><b>How it works?</b></p><p>irrE has been demonstrated to up regulate transcription of recA and pprA - genes which encode Recombines A and Radiation Inducible Protein. With respect to salt tolerance, irrE up regulates the production of several stress responsive proteins, protein kinases, metabolic proteins, and detoxification proteins. It also down regulates glycerol degradation. With this global regulatory effect, <i>E. coli</i> becomes more salt tolerant (UCL, 2012).</p><center><p><img width=429 height=322 src="https://static.igem.org/mediawiki/2014hs/9/9b/Imagen1cideb2014.png"align=center hspace=12 alt="IMG_0317"></p><p><b>Image 2.</b> This diagram shows the effect of irrE protein on <i>E. coli</i> metabolism. </p></center><p><b>How it is composed?</b></p><p>Initially, IrrE and L2+AIDA, protein for binding silica (<a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_union">Union module</a>), were joined together in only one circuit, but we needed to separate them becauseL2+AIDA has not been proved yet and it could affect the correct production of IrrE (see image 3).</p><center><p><img width=529 height=322 src="https://static.igem.org/mediawiki/2014hs/0/05/Circuit_l2_and_irre.png"align=center hspace=12 alt="IMG_0317"></p><p><b>Image 3.</b> Circuit for our project and for testing resistance and union modules. </p></center><br><p><b>Other teams that used it:</b></p><p><b><a href="https://2012.igem.org/Team:University_College_London">UCL 2012</a>:</b> They propose to confer salt tolerance on <i>E. coli</i> by linking the salt tolerance gene encoding the protein irrE (<a href="http://parts.igem.org/Part:BBa_K729001">BBa_K729001</a>) to a constitutive promoter (<a href="http://parts.igem.org/Part:BBa_J23119">BBa_J23119</a>). </p><center><p><img width=661 height=139 src="https://static.igem.org/mediawiki/2014hs/3/33/Gen1cideb2014.png"align=center hspace=12 alt="IMG_0317"></p><p><b>Image 3.</b> Part designed by <a href="https://2012.igem.org/Team:University_College_London">UCL 2012</a> for the irrE protein.</p></center><br><p><b>irrE's parts description :</b></p><br><center><div><table class=MsoTable15Grid7ColorfulAccent6 border=1 cellspacing=0 cellpadding=0 width=631 style='width:473.0pt;border-collapse:collapse; border:none;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'> <tr style='mso-yfti-irow:-1;mso-yfti-firstrow:yes;mso-yfti-lastfirstrow:yes; height:15.75pt'> <td width=112 nowrap valign=top style='width:84.0pt;border:none;border-bottom: solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint: 153;mso-border-bottom-alt:solid #FABF8F .5pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor: background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:517'><b><span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX;mso-bidi-font-style: italic'>IMAGE</span></b><b style='mso-bidi-font-weight:normal'><span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:"Times New Roman";mso-fareast-language: ES-MX;mso-bidi-font-style:italic'><o:p></o:p></span></b></p> </td> <td width=119 nowrap valign=top style='width:89.0pt;border:none;border-bottom: solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint: 153;mso-border-bottom-alt:solid #FABF8F .5pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor: background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:1'><b><span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX'>CODE</span></b><b style='mso-bidi-font-weight:normal'><span lang=ES-MX style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: "Times New Roman";mso-fareast-language:ES-MX'><o:p></o:p></span></b></p> </td> <td width=400 nowrap valign=top style='width:300.0pt;border:none;border-bottom: solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint: 153;mso-border-bottom-alt:solid #FABF8F .5pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor: background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:1'><b><span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX'>DESCRIPTION</span></b><b style='mso-bidi-font-weight:normal'><span lang=ES-MX style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: "Times New Roman";mso-fareast-language:ES-MX'><o:p></o:p></span></b></p> </td> </tr> <tr style='mso-yfti-irow:0;height:60.75pt'> <td width=112 nowrap valign=top style='width:84.0pt;border:none;border-right: solid #FABF8F 1.0pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; height:60.75pt'> <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:68'><!--[if gte vml 1]><v:shapetype id="_x0000_t75" coordsize="21600,21600" o:spt="75" o:preferrelative="t" path="m@4@5l@4@11@9@11@9@5xe" filled="f" stroked="f"> <v:stroke joinstyle="miter"/> <v:formulas> <v:f eqn="if lineDrawn pixelLineWidth 0"/> <v:f eqn="sum @0 1 0"/> <v:f eqn="sum 0 0 @1"/> <v:f eqn="prod @2 1 2"/> <v:f eqn="prod @3 21600 pixelWidth"/> <v:f eqn="prod @3 21600 pixelHeight"/> <v:f eqn="sum @0 0 1"/> <v:f eqn="prod @6 1 2"/> <v:f eqn="prod @7 21600 pixelWidth"/> <v:f eqn="sum @8 21600 0"/> <v:f eqn="prod @7 21600 pixelHeight"/> <v:f eqn="sum @10 21600 0"/> </v:formulas> <v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/> <o:lock v:ext="edit" aspectratio="t"/> </v:shapetype><v:shape id="_x0031__x0020_Imagen" o:spid="_x0000_s1033" type="#_x0000_t75" style='position:absolute;left:0;text-align:left; margin-left:15.9pt;margin-top:9.9pt;width:61.45pt;height:48.6pt;z-index:251649536; visibility:visible;mso-wrap-style:square;mso-width-percent:0; 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border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; mso-border-right-themecolor:accent6;mso-border-right-themetint:153; mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; background:#FDE9D9;mso-background-themecolor:accent6;mso-background-themetint: 51;padding:0cm 5.4pt 0cm 5.4pt;height:60.75pt'> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:64'><span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";color:black;mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_J23119">BBa_J23119</a><o:p></o:p></span></p> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:64'> </p> </td> <td width=400 valign=top style='width:300.0pt;border-top:none;border-left: none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;background:#FDE9D9;mso-background-themecolor:accent6; mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:60.75pt'> <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:normal;mso-yfti-cnfc:64'><span style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Arial;color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>In the specific case of our bacteria, it helps to continuously transcribing the <span class=SpellE>irrE</span> gene in order to make bacteria resist high concentration of salt. </span><span class=SpellE><span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:Arial;color:#282828;mso-fareast-language:ES-MX'>This</span></span><span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Arial;color:#282828;mso-fareast-language: ES-MX'> <span class=SpellE>promoter</span> has a <span class=SpellE>length</span> of 35 <span class=SpellE>pb</span>(Anderson, 2006).<o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:1;height:75.0pt'> <td width=112 nowrap valign=top style='width:84.0pt;border-top:solid #FABF8F 1.0pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;border-left: none;border-bottom:none;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; height:75.0pt'> <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:4'><!--[if gte vml 1]><v:shape id="_x0032__x0020_Imagen" o:spid="_x0000_s1032" type="#_x0000_t75" style='position:absolute; left:0;text-align:left;margin-left:23.1pt;margin-top:6.95pt;width:54.25pt; height:49.45pt;z-index:251651584;visibility:visible;mso-wrap-style:square; mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt; mso-wrap-distance-top:0;mso-wrap-distance-right:9pt; mso-wrap-distance-bottom:0;mso-position-horizontal:absolute; mso-position-horizontal-relative:text;mso-position-vertical:absolute; mso-position-vertical-relative:text;mso-width-percent:0; mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'> <v:imagedata src="TablasAromaYResistencia_archivos/image003.png" o:title=""/> <w:wrap type="square"/> </v:shape><![endif]--><![if !vml]><img width=72 height=66 src="https://static.igem.org/mediawiki/2014hs/5/5d/Cds.png" align=left hspace=12 v:shapes="_x0032__x0020_Imagen"><![endif]><i><span style='font-family:Oxygen; color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191;mso-ansi-language: EN-US;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p></span></i></p> <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:4'> </p> </td> <td width=119 nowrap valign=top style='width:89.0pt;border-top:none; border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; mso-border-right-themecolor:accent6;mso-border-right-themetint:153; mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; padding:0cm 5.4pt 0cm 5.4pt;height:75.0pt'> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal'><span style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: "Times New Roman";color:black;mso-ansi-language:EN-US;mso-fareast-language: ES-MX'><a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a><o:p></o:p></span></p> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal'> </p> </td> <td width=400 valign=top style='width:300.0pt;border-top:none;border-left: none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;padding:0cm 5.4pt 0cm 5.4pt;height:75.0pt'> <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:normal'><span style='font-size:12.0pt;font-family:Oxygen; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Arial; color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>This specific is <span class=SpellE>RBS</span> based on <span class=SpellE>Elowitz</span> <span class=SpellE>repressilator</span>. It is very common to see in many <span class=SpellE>iGEM</span> projects.<span style='mso-spacerun:yes'> </span>It has a length of 12 <span class=SpellE>pb </span>(Mahajan, <span class=SpellE>Marinescu</span>, Chow, <span class=SpellE>Wissner</span>-Gross, & Carr, 2003).<o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:2;height:90.35pt'> <td width=112 nowrap valign=top style='width:84.0pt;border-top:solid #FABF8F 1.0pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;border-left: none;border-bottom:none;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; height:90.35pt'> <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:68'><!--[if gte vml 1]><v:shape id="_x0033__x0020_Imagen" o:spid="_x0000_s1031" type="#_x0000_t75" style='position:absolute; left:0;text-align:left;margin-left:23.4pt;margin-top:10pt;width:57pt; height:48pt;z-index:251654656;visibility:visible;mso-wrap-style:square; mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt; mso-wrap-distance-top:0;mso-wrap-distance-right:9pt; mso-wrap-distance-bottom:0;mso-position-horizontal:absolute; mso-position-horizontal-relative:text;mso-position-vertical:absolute; mso-position-vertical-relative:text;mso-width-percent:0; mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'> <v:imagedata src="TablasAromaYResistencia_archivos/image005.png" o:title=""/> <w:wrap type="square"/> </v:shape><![endif]--><![if !vml]><img width=76 height=64 src="https://static.igem.org/mediawiki/2014hs/9/97/IrrEResistance.png" align=left hspace=12 v:shapes="_x0033__x0020_Imagen"><![endif]></p> </td> <td width=119 nowrap valign=top style='width:89.0pt;border-top:none; border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; mso-border-right-themecolor:accent6;mso-border-right-themetint:153; mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; background:#FDE9D9;mso-background-themecolor:accent6;mso-background-themetint: 51;padding:0cm 5.4pt 0cm 5.4pt;height:90.35pt'> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:64'><span style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";color:black;mso-ansi-language:EN-US; mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_K729001">BBa_K729001</a><o:p></o:p></span></p> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:64'><span style='font-size:3.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";color:black;mso-ansi-language:EN-US; mso-fareast-language:ES-MX'><o:p></o:p></span></p> </td> <td width=400 valign=top style='width:300.0pt;border-top:none;border-left: none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;background:#FDE9D9;mso-background-themecolor:accent6; mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:90.35pt'> <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:normal;mso-yfti-cnfc:64'><span style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Arial;color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>Gene that produces <span class=SpellE>irrE</span>, a substance that changes the bacteria’s metabolism and allows bacteria to survive to extreme conditions, some examples could be high UV rays exposition, or high salt concentration levels in an aquatic environment, oxidative or thermal shock.<span style='mso-spacerun:yes'> </span>It has a length of 933pb (<span class=SpellE>Sohrabi</span>, 2012).<o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:3;mso-yfti-lastrow:yes;height:62.25pt'> <td width=112 nowrap valign=top style='width:84.0pt;border-top:solid #FABF8F 1.0pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;border-left: none;border-bottom:none;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; height:62.25pt'> <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:4'><!--[if gte vml 1]><v:shape id="_x0034__x0020_Imagen" o:spid="_x0000_s1030" type="#_x0000_t75" style='position:absolute; left:0;text-align:left;margin-left:22.6pt;margin-top:5.5pt;width:54.75pt; height:52.5pt;z-index:251657728;visibility:visible;mso-wrap-style:square; mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt; mso-wrap-distance-top:0;mso-wrap-distance-right:9pt; mso-wrap-distance-bottom:0;mso-position-horizontal:absolute; mso-position-horizontal-relative:text;mso-position-vertical:absolute; mso-position-vertical-relative:text;mso-width-percent:0; mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'> <v:imagedata src="TablasAromaYResistencia_archivos/image006.png" o:title=""/> <w:wrap type="square"/> </v:shape><![endif]--><![if !vml]><img width=73 height=70 src="https://static.igem.org/mediawiki/2014hs/5/55/TerminatorResistance.png" align=left hspace=12 v:shapes="_x0034__x0020_Imagen"><![endif]><i><span style='font-family:Oxygen; color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191;mso-ansi-language: EN-US;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p></span></i><i><span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:"Times New Roman";color:black; mso-fareast-language:ES-MX'> <o:p></o:p> </span></i></p> </td> <td width=119 nowrap valign=top style='width:89.0pt;border-top:none; border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; mso-border-right-themecolor:accent6;mso-border-right-themetint:153; mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; padding:0cm 5.4pt 0cm 5.4pt;height:62.25pt'> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal'><span lang=ES-MX style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: "Times New Roman";color:black;mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_B1002">BBa_B1002</a><o:p></o:p></span></p> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal'> </p></td> <td width=400 valign=top style='width:300.0pt;border-top:none;border-left: none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;padding:0cm 5.4pt 0cm 5.4pt;height:62.25pt'> <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:normal'><span style='font-size:12.0pt;font-family:Oxygen; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman"; color:black;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>Part made of 6pb responsible for transcription stop (Huang, 206).<o:p></o:p></span></p> </td> </tr></table></div></center><p><h2><b>Project Zoom In</b></h2></p></br><center><iframe width="600" height="500" src="//www.youtube.com/embed/qAYmPrq9NsY" frameborder="0" allowfullscreen></iframe></center><br><p><b><h2>Bibliography/References</h2></b></p><font size="2"><p>● Antiquity 2013. (2013, January 31). <i>Part:BBa_B0034</i>. Retrieved from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034">http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034</a>.</p><p>● Huang, H. (2006, August 30). <i>Part:BBa_B1002</i>. Retrieved August 30, 2014, from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B1002">http://parts.igem.org/wiki/index.php?title=Part:BBa_B1002</a>.</p><p>● iGEM2006_Berkeley. (2006, August 24). <i>Part:BBa_J23119</i>. Retrieved April 30, 2014, from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_J23119">http://parts.igem.org/wiki/index.php?title=Part:BBa_J23119</a>.</p><p>● Sohrabi, B. (2012, June 27). <i>Part:BBa_K729001</i>. Retrieved from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K729001">http://parts.igem.org/wiki/index.php?title=Part:BBa_K729001</a>.</p><p>● UCLiGEM Team. (2012). <i>IRRE module</i>. Retrieved March 31, 2014. from <a href="https://2012.igem.org/Team:University_College_London/Module_5">https://2012.igem.org/Team:University_College_London/Module_5</a>.</p></font><br><div style="text-align: right;"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_resistance#"><font color="blue">Return to the Top</font></a></p></div></div></div></body></html>{{:Team:CIDEB-UANL_Mexico/footer}}	+	{{:Team:CIDEB-UANL_Mexico/style2.css}}{{:Team:CIDEB-UANL_Mexico/menu_project}}<html xmlns="http://www.w3.org/1999/xhtml"><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><link href='http://fonts.googleapis.com/css?family=Oxygen:400,700' rel='stylesheet' type='text/css'><title>iGEM CIDEB 2014 - Project</title><style>body{margin: 0px; width: 100%;padding: 0px;background: #2056ac;font-family: 'Oxygen', sans-serif;font-size: 12pt;background-image:url(https://static.igem.org/mediawiki/2014hs/3/32/Cideb_Fondo1.gif);}h1, h2, h3{margin: 0;padding-bottom: 5px;color: #404040;}p, ol, ul{margin-top: 0;}ol, ul{padding: 0;list-style: none;}p{line-height: 1.60em;padding- right: 3em;}strong{}a{color: #2056ac;}a:hover{text-decoration: none;}.container{margin: 0px auto;width: 1200px;}.container-text{margin: 0px auto;width: 75%;padding: 0px;font-family: 'Oxygen', sans-serif;font-size: 12pt; text-align: justify;}.wrapper{overflow: hidden;padding: 0em 0em 1em 0em;background: #FFF;}#wrapper1{background: #FFF;}#wrapper2{overflow: hidden;background: #F3F3F3;padding: 5em 0em;text-align: center;}#wrapper3{overflow: hidden;padding: 0em 0em 0em 0em;background: #FFF;}#wrapper4{}#banner{padding-top: 2em;}#welcome{overflow: hidden;width: 1000px;padding: 0em 100px 0em 100px;text-align: center;}#welcome .content{padding: 0em 8em;}#welcome .title h2{}#welcome a,#welcome strong{}.title{margin-bottom: 1em;}.title h2{font-size: 2em;}.title .byline{font-size: 1.1em;color: #6F6F6F#;}#three-column{overflow: hidden;margin-top: 5em;padding-top: 1em;border-top: 1px solid rgba(0,0,0,0.2);text-align: center;}#three-column h2{margin: 1em 0em;font-size: 1.5em;font-weight: 700;}#three-column .icon{position: relative;display: block;margin: 0px auto 0.80em auto;background: none;line-height: 150px;font-size: 4em;width: 150px;height: 100px;border-radius: 100px;border: 6px solid #67128F;text-align: center;color: #FFF;}#three-column #tbox1,#three-column #tbox2,#three-column #tbox3{float: left;width: 320px;padding: 30px 40px 50px 40px;}#three-column .title{text-align: center;}#three-column .title h2{font-size: 1.60em;}#three-column .title .byline{padding-top: 0.50em;font-size: 0.90em;color: #858585;}#three-column .arrow-down{border-top-color: #292929;}ul.tools{margin: 0;padding: 0em 0em 0em 0em;list-style: none;}ul.tools li{display: inline-block;padding: 0em .2em;font-size: 4em;}ul.tools li span{display: none;margin: 0;padding: 0;}ul.tools li a{color: #FFF;}ul.tools li a:before{display: inline-block;background: #1ABC9C;width: 120px;height: 120px;border-radius: 50%;line-height: 120px;text-align: center;color: #FFFFFF;}.button{display: inline-block;margin-top: 2em;padding: 0.8em 2em;background: #64ABD1;line-height: 1.8em;letter-spacing: 1px;text-decoration: none;font-size: 1em;color: #FFF;}.button:before{display: inline-block;background: #8DCB89;margin-right: 1em;width: 40px;height: 40px;line-height: 40px;border-radius: 20px;text-align: center;color: #272925;}.button-small{}#portfolio{overflow: hidden;padding-top: 5em;border-top: 1px solid rgba(0,0,0,0.2);}#portfolio .box{text-align: center;color: rgba(0,0,0,0.5);}#portfolio h3{display: block;padding-bottom: 1em;font-size: 1em;color: rgba(0,0,0,0.6);}#portfolio .title{text-align: center;}#portfolio .title h2{color: rgba(0,0,0,0.8);}.column1,.column2,.column3,.column4{width: 282px;}.column1,.column2,.column3{float: left;margin-right: 24px;}.column4{float: right;}</style><body><div class="wrapper"><div id="welcome" class="container"> <div class="title"> <h2>Resistance Module</h2> </div></div><div class="container-text"><table width=100%><tr><td><p>Because our project is a biofilter which allows to remove salt from water, the bacteria needs to survive to extreme environmental conditions which normally it can’t do. We need <i>E. coli</i> to survive a high salinity environment to allow it to capture sodium ions and to remove the salt concentration of the water.</p><p>In order to accomplish our project goal, we have to change <i>E. coli</i> metabolism and make it stronger, more resistant and more efficient than in normal <i>E. coli</i> bacteria.</p></td><td style="padding-left:12px;"><img width=131 height=140 src="https://static.igem.org/mediawiki/2014hs/8/8e/BeutifulResistance.jpg"/></td></tr></table><p>Last discoveries show irrE as a protein capable of change the <i>E. coli</i> metabolism, and giving it the ability to survive to bigger temperatures, bigger UV rays radiation and bigger salt concentration (UCL, 2012).</p><br><p><b>What does irrE do?</b></p><p>The protein irrE originates from <i>Deinococcus radiodurans</i>, and initially, this gene provides resistance to radiation. But when transformed in <i>E. coli</i>, it protects it against salt, oxidative and thermal shock. (UCL, 2012) Also, different experiments from different iGEM teams, support the idea of the bigger salt resistance in <i>E. coli</i> with this biobrick. </p><center><p><img width=564 height=350 src="https://static.igem.org/mediawiki/2014hs/3/3e/Grafica_1_cideb2014.png"align=center hspace=12 alt="IMG_0317"></p><p><b>Image 1.</b> This graph done by <a herf="https://2012.igem.org/Team:University_College_London">UCL iGEM 2012</a>, shows how irrE biobrick increased the salt concentration resistance in <i>E. coli</i> compared with the results from <a href="https://2010.igem.org/Team:TU_Delft">Tu Delf iGEM 2010</a>.</p></center><br><p><b>How it works?</b></p><p>irrE has been demonstrated to up regulate transcription of recA and pprA - genes which encode Recombines A and Radiation Inducible Protein. With respect to salt tolerance, irrE up regulates the production of several stress responsive proteins, protein kinases, metabolic proteins, and detoxification proteins. It also down regulates glycerol degradation. With this global regulatory effect, <i>E. coli</i> becomes more salt tolerant (UCL, 2012).</p><center><p><img width=429 height=322 src="https://static.igem.org/mediawiki/2014hs/9/9b/Imagen1cideb2014.png"align=center hspace=12 alt="IMG_0317"></p><p><b>Image 2.</b> This diagram shows the effect of irrE protein on <i>E. coli</i> metabolism. </p></center><p><b>How it is composed?</b></p><p>Initially, IrrE and L2+AIDA, protein for binding silica (<a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_union">Union module</a>), were joined together in only one circuit, but we needed to separate them becauseL2+AIDA has not been proved yet and it could affect the correct production of IrrE (see image 3).</p><center><p><img width=529 height=322 src="https://static.igem.org/mediawiki/2014hs/0/05/Circuit_l2_and_irre.png"align=center hspace=12 alt="IMG_0317"></p><p><b>Image 3.</b> Circuit for our project and for testing resistance and union modules. </p></center><br><p><b>Other teams that used it:</b></p><p><b><a href="https://2012.igem.org/Team:University_College_London">UCL 2012</a>:</b> They propose to confer salt tolerance on <i>E. coli</i> by linking the salt tolerance gene encoding the protein irrE (<a href="http://parts.igem.org/Part:BBa_K729001">BBa_K729001</a>) to a constitutive promoter (<a href="http://parts.igem.org/Part:BBa_J23119">BBa_J23119</a>). </p><center><p><img width=661 height=139 src="https://static.igem.org/mediawiki/2014hs/3/33/Gen1cideb2014.png"align=center hspace=12 alt="IMG_0317"></p><p><b>Image 3.</b> Part designed by <a href="https://2012.igem.org/Team:University_College_London">UCL 2012</a> for the irrE protein.</p></center><br><p><b>irrE's parts description :</b></p><br><center><div><table class=MsoTable15Grid7ColorfulAccent6 border=1 cellspacing=0 cellpadding=0 width=631 style='width:473.0pt;border-collapse:collapse; border:none;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'> <tr style='mso-yfti-irow:-1;mso-yfti-firstrow:yes;mso-yfti-lastfirstrow:yes; height:15.75pt'> <td width=112 nowrap valign=top style='width:84.0pt;border:none;border-bottom: solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint: 153;mso-border-bottom-alt:solid #FABF8F .5pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor: background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:517'><b><span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX;mso-bidi-font-style: italic'>IMAGE</span></b><b style='mso-bidi-font-weight:normal'><span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:"Times New Roman";mso-fareast-language: ES-MX;mso-bidi-font-style:italic'><o:p></o:p></span></b></p> </td> <td width=119 nowrap valign=top style='width:89.0pt;border:none;border-bottom: solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint: 153;mso-border-bottom-alt:solid #FABF8F .5pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor: background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:1'><b><span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX'>CODE</span></b><b style='mso-bidi-font-weight:normal'><span lang=ES-MX style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: "Times New Roman";mso-fareast-language:ES-MX'><o:p></o:p></span></b></p> </td> <td width=400 nowrap valign=top style='width:300.0pt;border:none;border-bottom: solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint: 153;mso-border-bottom-alt:solid #FABF8F .5pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor: background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:1'><b><span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX'>DESCRIPTION</span></b><b style='mso-bidi-font-weight:normal'><span lang=ES-MX style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: "Times New Roman";mso-fareast-language:ES-MX'><o:p></o:p></span></b></p> </td> </tr> <tr style='mso-yfti-irow:0;height:60.75pt'> <td width=112 nowrap valign=top style='width:84.0pt;border:none;border-right: solid #FABF8F 1.0pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; height:60.75pt'> <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:68'><!--[if gte vml 1]><v:shapetype id="_x0000_t75" coordsize="21600,21600" o:spt="75" o:preferrelative="t" path="m@4@5l@4@11@9@11@9@5xe" filled="f" stroked="f"> <v:stroke joinstyle="miter"/> <v:formulas> <v:f eqn="if lineDrawn pixelLineWidth 0"/> <v:f eqn="sum @0 1 0"/> <v:f eqn="sum 0 0 @1"/> <v:f eqn="prod @2 1 2"/> <v:f eqn="prod @3 21600 pixelWidth"/> <v:f eqn="prod @3 21600 pixelHeight"/> <v:f eqn="sum @0 0 1"/> <v:f eqn="prod @6 1 2"/> <v:f eqn="prod @7 21600 pixelWidth"/> <v:f eqn="sum @8 21600 0"/> <v:f eqn="prod @7 21600 pixelHeight"/> <v:f eqn="sum @10 21600 0"/> </v:formulas> <v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/> <o:lock v:ext="edit" aspectratio="t"/> </v:shapetype><v:shape id="_x0031__x0020_Imagen" o:spid="_x0000_s1033" type="#_x0000_t75" style='position:absolute;left:0;text-align:left; margin-left:15.9pt;margin-top:9.9pt;width:61.45pt;height:48.6pt;z-index:251649536; visibility:visible;mso-wrap-style:square;mso-width-percent:0; mso-height-percent:0;mso-wrap-distance-left:9pt;mso-wrap-distance-top:0; mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:0; mso-position-horizontal:absolute;mso-position-horizontal-relative:text; mso-position-vertical:absolute;mso-position-vertical-relative:text; mso-width-percent:0;mso-height-percent:0;mso-width-relative:page; mso-height-relative:page'> <v:imagedata src="TablasAromaYResistencia_archivos/image001.png" o:title=""/> <w:wrap type="square"/> </v:shape><![endif]--><![if !vml]><img width=82 height=65 src="https://static.igem.org/mediawiki/2014hs/6/62/Promotor.png" align=left hspace=12 v:shapes="_x0031__x0020_Imagen"><![endif]><i><span lang=ES-MX style='font-family:Oxygen;color:#E36C0A;mso-themecolor:accent6;mso-themeshade: 191;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p></span></i></p> <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:68'> </p></td> <td width=119 nowrap valign=top style='width:89.0pt;border-top:none; border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; mso-border-right-themecolor:accent6;mso-border-right-themetint:153; mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; background:#FDE9D9;mso-background-themecolor:accent6;mso-background-themetint: 51;padding:0cm 5.4pt 0cm 5.4pt;height:60.75pt'> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:64'><span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";color:black;mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_J23119">BBa_J23119</a><o:p></o:p></span></p> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:64'> </p> </td> <td width=400 valign=top style='width:300.0pt;border-top:none;border-left: none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;background:#FDE9D9;mso-background-themecolor:accent6; mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:60.75pt'> <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:normal;mso-yfti-cnfc:64'><span style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Arial;color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>In the specific case of our bacteria, it helps to continuously transcribing the <span class=SpellE>irrE</span> gene in order to make bacteria resist high concentration of salt. </span><span class=SpellE><span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:Arial;color:#282828;mso-fareast-language:ES-MX'>This</span></span><span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Arial;color:#282828;mso-fareast-language: ES-MX'> <span class=SpellE>promoter</span> has a <span class=SpellE>length</span> of 35 <span class=SpellE>pb</span>(Anderson, 2006).<o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:1;height:75.0pt'> <td width=112 nowrap valign=top style='width:84.0pt;border-top:solid #FABF8F 1.0pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;border-left: none;border-bottom:none;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; height:75.0pt'> <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:4'><!--[if gte vml 1]><v:shape id="_x0032__x0020_Imagen" o:spid="_x0000_s1032" type="#_x0000_t75" style='position:absolute; left:0;text-align:left;margin-left:23.1pt;margin-top:6.95pt;width:54.25pt; height:49.45pt;z-index:251651584;visibility:visible;mso-wrap-style:square; mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt; mso-wrap-distance-top:0;mso-wrap-distance-right:9pt; mso-wrap-distance-bottom:0;mso-position-horizontal:absolute; mso-position-horizontal-relative:text;mso-position-vertical:absolute; mso-position-vertical-relative:text;mso-width-percent:0; mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'> <v:imagedata src="TablasAromaYResistencia_archivos/image003.png" o:title=""/> <w:wrap type="square"/> </v:shape><![endif]--><![if !vml]><img width=72 height=66 src="https://static.igem.org/mediawiki/2014hs/5/5d/Cds.png" align=left hspace=12 v:shapes="_x0032__x0020_Imagen"><![endif]><i><span style='font-family:Oxygen; color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191;mso-ansi-language: EN-US;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p></span></i></p> <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:4'> </p> </td> <td width=119 nowrap valign=top style='width:89.0pt;border-top:none; border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; mso-border-right-themecolor:accent6;mso-border-right-themetint:153; mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; padding:0cm 5.4pt 0cm 5.4pt;height:75.0pt'> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal'><span style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: "Times New Roman";color:black;mso-ansi-language:EN-US;mso-fareast-language: ES-MX'><a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a><o:p></o:p></span></p> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal'> </p> </td> <td width=400 valign=top style='width:300.0pt;border-top:none;border-left: none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;padding:0cm 5.4pt 0cm 5.4pt;height:75.0pt'> <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:normal'><span style='font-size:12.0pt;font-family:Oxygen; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Arial; color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>This specific is <span class=SpellE>RBS</span> based on <span class=SpellE>Elowitz</span> <span class=SpellE>repressilator</span>. It is very common to see in many <span class=SpellE>iGEM</span> projects.<span style='mso-spacerun:yes'> </span>It has a length of 12 <span class=SpellE>pb </span>(Mahajan, <span class=SpellE>Marinescu</span>, Chow, <span class=SpellE>Wissner</span>-Gross, & Carr, 2003).<o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:2;height:90.35pt'> <td width=112 nowrap valign=top style='width:84.0pt;border-top:solid #FABF8F 1.0pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;border-left: none;border-bottom:none;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; height:90.35pt'> <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:68'><!--[if gte vml 1]><v:shape id="_x0033__x0020_Imagen" o:spid="_x0000_s1031" type="#_x0000_t75" style='position:absolute; left:0;text-align:left;margin-left:23.4pt;margin-top:10pt;width:57pt; height:48pt;z-index:251654656;visibility:visible;mso-wrap-style:square; mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt; mso-wrap-distance-top:0;mso-wrap-distance-right:9pt; mso-wrap-distance-bottom:0;mso-position-horizontal:absolute; mso-position-horizontal-relative:text;mso-position-vertical:absolute; mso-position-vertical-relative:text;mso-width-percent:0; mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'> <v:imagedata src="TablasAromaYResistencia_archivos/image005.png" o:title=""/> <w:wrap type="square"/> </v:shape><![endif]--><![if !vml]><img width=76 height=64 src="https://static.igem.org/mediawiki/2014hs/9/97/IrrEResistance.png" align=left hspace=12 v:shapes="_x0033__x0020_Imagen"><![endif]></p> </td> <td width=119 nowrap valign=top style='width:89.0pt;border-top:none; border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; mso-border-right-themecolor:accent6;mso-border-right-themetint:153; mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; background:#FDE9D9;mso-background-themecolor:accent6;mso-background-themetint: 51;padding:0cm 5.4pt 0cm 5.4pt;height:90.35pt'> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:64'><span style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";color:black;mso-ansi-language:EN-US; mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_K729001">BBa_K729001</a><o:p></o:p></span></p> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:64'><span style='font-size:3.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";color:black;mso-ansi-language:EN-US; mso-fareast-language:ES-MX'><o:p></o:p></span></p> </td> <td width=400 valign=top style='width:300.0pt;border-top:none;border-left: none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;background:#FDE9D9;mso-background-themecolor:accent6; mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:90.35pt'> <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:normal;mso-yfti-cnfc:64'><span style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Arial;color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>Gene that produces <span class=SpellE>irrE</span>, a substance that changes the bacteria’s metabolism and allows bacteria to survive to extreme conditions, some examples could be high UV rays exposition, or high salt concentration levels in an aquatic environment, oxidative or thermal shock.<span style='mso-spacerun:yes'> </span>It has a length of 933pb (<span class=SpellE>Sohrabi</span>, 2012).<o:p></o:p></span></p> </td> </tr> <tr style='mso-yfti-irow:3;mso-yfti-lastrow:yes;height:62.25pt'> <td width=112 nowrap valign=top style='width:84.0pt;border-top:solid #FABF8F 1.0pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;border-left: none;border-bottom:none;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; height:62.25pt'> <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:4'><!--[if gte vml 1]><v:shape id="_x0034__x0020_Imagen" o:spid="_x0000_s1030" type="#_x0000_t75" style='position:absolute; left:0;text-align:left;margin-left:22.6pt;margin-top:5.5pt;width:54.75pt; height:52.5pt;z-index:251657728;visibility:visible;mso-wrap-style:square; mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt; mso-wrap-distance-top:0;mso-wrap-distance-right:9pt; mso-wrap-distance-bottom:0;mso-position-horizontal:absolute; mso-position-horizontal-relative:text;mso-position-vertical:absolute; mso-position-vertical-relative:text;mso-width-percent:0; mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'> <v:imagedata src="TablasAromaYResistencia_archivos/image006.png" o:title=""/> <w:wrap type="square"/> </v:shape><![endif]--><![if !vml]><img width=73 height=70 src="https://static.igem.org/mediawiki/2014hs/5/55/TerminatorResistance.png" align=left hspace=12 v:shapes="_x0034__x0020_Imagen"><![endif]><i><span style='font-family:Oxygen; color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191;mso-ansi-language: EN-US;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p></span></i><i><span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:"Times New Roman";color:black; mso-fareast-language:ES-MX'> <o:p></o:p> </span></i></p> </td> <td width=119 nowrap valign=top style='width:89.0pt;border-top:none; border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; mso-border-right-themecolor:accent6;mso-border-right-themetint:153; mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; padding:0cm 5.4pt 0cm 5.4pt;height:62.25pt'> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal'><span lang=ES-MX style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: "Times New Roman";color:black;mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_B1002">BBa_B1002</a><o:p></o:p></span></p> <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal'> </p></td> <td width=400 valign=top style='width:300.0pt;border-top:none;border-left: none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;padding:0cm 5.4pt 0cm 5.4pt;height:62.25pt'> <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:normal'><span style='font-size:12.0pt;font-family:Oxygen; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman"; color:black;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>Part made of 6pb responsible for transcription stop (Huang, 206).<o:p></o:p></span></p> </td> </tr></table></div></center><p><h2><b>Project Zoom In</b></h2></p></br><center><iframe width="600" height="500" src="//www.youtube.com/embed/qAYmPrq9NsY" frameborder="0" allowfullscreen></iframe></center><br><p><b><h2>Bibliography/References</h2></b></p><font size="2"><p>● Antiquity 2013. (2013, January 31). <i>Part:BBa_B0034</i>. Retrieved from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034">http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034</a>.</p><p>● Huang, H. (2006, August 30). <i>Part:BBa_B1002</i>. Retrieved August 30, 2014, from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B1002">http://parts.igem.org/wiki/index.php?title=Part:BBa_B1002</a>.</p><p>● iGEM2006_Berkeley. (2006, August 24). <i>Part:BBa_J23119</i>. Retrieved April 30, 2014, from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_J23119">http://parts.igem.org/wiki/index.php?title=Part:BBa_J23119</a>.</p><p>● Sohrabi, B. (2012, June 27). <i>Part:BBa_K729001</i>. Retrieved from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K729001">http://parts.igem.org/wiki/index.php?title=Part:BBa_K729001</a>.</p><p>● UCLiGEM Team. (2012). <i>IRRE module</i>. Retrieved March 31, 2014. from <a href="https://2012.igem.org/Team:University_College_London/Module_5">https://2012.igem.org/Team:University_College_London/Module_5</a>.</p></font><br><div style="text-align: right;"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_resistance#"><font color="blue">Return to the Top</font></a></p></div></div></div></body></html>{{:Team:CIDEB-UANL_Mexico/footer}}