Team:TP CC-SanDiego/Notebook/Journal/Test

From 2014hs.igem.org

(Difference between revisions)
Line 14: Line 14:
<!-- Place favicon.ico and apple-touch-icon.png in the root directory -->
<!-- Place favicon.ico and apple-touch-icon.png in the root directory -->
 +
<style>
 +
/*
 +
* HTML5 Boilerplate
 +
*
 +
* What follows is the result of much research on cross-browser styling.
 +
* Credit left inline and big thanks to Nicolas Gallagher, Jonathan Neal,
 +
* Kroc Camen, and the H5BP dev community and team.
 +
*/
-
+
/* ==========================================================================
-
+
  Base styles: opinionated defaults
-
+
  ========================================================================== */
-
+
 
-
+
 
-
<link rel="stylesheet" href="https://googledrive.com/host/0B48OkScOEMY3cXg1UUx5UVlxRHM/css/main.css">
+
/*
 +
* Remove text-shadow in selection highlight: h5bp.com/i
 +
* These selection declarations have to be separate.
 +
* Customize the background color to match your design.
 +
*/
 +
 
 +
::-moz-selection {
 +
text-shadow: none;
 +
}
 +
 
 +
::selection {
 +
text-shadow: none;
 +
}
 +
 
 +
/*
 +
* A better looking default horizontal rule
 +
*/
 +
 
 +
hr {
 +
display: block;
 +
height: 1px;
 +
border: 0;
 +
border-top: 1px solid #ccc;
 +
margin: 1em 0;
 +
padding: 0;
 +
}
 +
 
 +
/*
 +
* Remove the gap between images and the bottom of their containers: h5bp.com/i/440
 +
*/
 +
 
 +
img {
 +
vertical-align: middle;
 +
}
 +
 
 +
/*
 +
* Remove default fieldset styles.
 +
*/
 +
 
 +
fieldset {
 +
border: 0;
 +
margin: 0;
 +
padding: 0;
 +
}
 +
 
 +
/*
 +
* Allow only vertical resizing of textareas.
 +
*/
 +
 
 +
textarea {
 +
resize: vertical;
 +
}
 +
 
 +
/* ==========================================================================
 +
  Chrome Frame prompt
 +
  ========================================================================== */
 +
 
 +
.chromeframe {
 +
margin: 0.2em 0;
 +
background: #ccc;
 +
color: #000;
 +
padding: 0.2em 0;
 +
}
 +
 
 +
 
 +
/* ==========================================================================
 +
  Basic layout
 +
  ========================================================================== */
 +
 
 +
@media only screen and (-webkit-min-device-pixel-ratio: 2) {
 +
body {
 +
background-size: 50%;
 +
}
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}
 +
 
 +
.site-header {
 +
min-width: 280px;
 +
max-width: 826px;
 +
margin: 0 auto;
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}
 +
 
 +
.site-header {
 +
padding: 0 0 42px;
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}
 +
 
 +
.site-header .tagline {
 +
margin: 0;
 +
}
 +
 
 +
.sparator {
 +
width: 100%;
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}
 +
 
 +
.site-header, .about-me, .from-my-blog {
 +
width: 75%;
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}
 +
 
 +
@media all and (min-width:601px) and (max-width:1020px) {
 +
.site-header, .about-me, .from-my-blog {
 +
width: 82%;
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}
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}
 +
 
 +
.works, .works-hex, nav, .blog-excerpts-wrapper {
 +
background-color: rgba(0,0,0,0.4);
 +
}
 +
 
 +
 
 +
/* ==========================================================================
 +
  Navigation
 +
  ========================================================================== */
 +
 
 +
nav {
 +
height: 78px;
 +
padding-bottom: 5px;
 +
}
 +
 
 +
.top-ribbon {
 +
height: 5px;
 +
}
 +
 
 +
nav ul {
 +
width: 75%;
 +
min-width: 280px;
 +
max-width: 826px;
 +
margin: 0 auto;
 +
padding: 0;
 +
}
 +
 
 +
nav li {
 +
float: right;
 +
display: inline-block;
 +
padding: 15px 10px 2px;
 +
margin: 0 6px;
 +
-webkit-border-radius: 0 0 7px 7px;
 +
-moz-border-radius: 0 0 7px 7px;
 +
border-radius: 0 0 7px 7px;
 +
cursor: pointer;
 +
}
 +
 
 +
.win nav li {
 +
padding: 12px 10px 5px;
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}
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nav li:hover {
 +
background-color: rgb(192, 23, 104);
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text-shadow: 1px 1px 3px rgb(0, 0, 0);
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}
 +
 
 +
 
 +
/* ==========================================================================
 +
  Hexagon shape defaults
 +
  ========================================================================== */
 +
 
 +
.hexagon {
 +
display: inline-block;
 +
overflow: hidden;
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visibility: hidden;
 +
-webkit-transform: rotate(120deg);
 +
  -moz-transform: rotate(120deg);
 +
transform: rotate(120deg);
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}
 +
 
 +
.hexagon-in1, .hexagon-in2 {
 +
overflow: hidden;
 +
display: block;
 +
width: 100%;
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height: 100%;
 +
-webkit-transform: rotate(-60deg);
 +
  -moz-transform: rotate(-60deg);
 +
transform: rotate(-60deg);
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}
 +
 
 +
.hexagon-in2 {
 +
overflow: hidden;
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visibility: visible;
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background-color: rgba(46, 46, 46, 1);
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}
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/* ==========================================================================
 +
  Works & Gallery effects
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  ========================================================================== */
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 +
@media all and (min-width:1031px) {
 +
 
 +
.thumbnail {
 +
-webkit-transition: all 0.2s ease-out;
 +
  -moz-transition: all 0.2s ease-out;
 +
transition: all 0.2s ease-out;
 +
}
 +
 
 +
.thumbnail:hover {
 +
-webkit-transition: all 0.2s ease-out;
 +
  -moz-transition: all 0.2s ease-out;
 +
transition: all 0.2s ease-out;
 +
}
 +
 
 +
.works-hex .thumbnail:hover, .gallery-hex .thumbnail:hover {
 +
width: 110%;
 +
margin: 0 0 0 -5%;
 +
}
 +
 
 +
.works .thumbnail, .gallery .thumbnail {
 +
-webkit-filter: grayscale(1) sepia(0.03);
 +
}
 +
 
 +
.works .thumbnail:hover , .gallery .thumbnail:hover {
 +
-webkit-filter: grayscale(0) sepia(0);
 +
}
 +
}
 +
 
 +
 
 +
/* ==========================================================================
 +
  Works
 +
  ========================================================================== */
 +
 
 +
.thumbnail {
 +
width: 100%;
 +
}
 +
 
 +
.works, .works-hex {
 +
text-align: center;
 +
overflow: hidden;
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}
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 +
.category {
 +
border: none;
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display: inline-block;
 +
padding-bottom: 2%;
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}
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 +
.category:hover {
 +
border: none;
 +
}
 +
 
 +
.works .category {
 +
width: 23%;
 +
min-width: 90px;
 +
max-width: 256px;
 +
margin: 0 12px;
 +
}
 +
 
 +
.four.works .category {
 +
width: 17%;
 +
min-width: 72px;
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max-width: 186px;
 +
margin: 0 11px;
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}
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.works-hex .category {
 +
width: 25%;
 +
min-width: 100px;
 +
max-width: 270px;
 +
margin: 0 1px;
 +
}
 +
 
 +
@media all and (max-width:480px) {
 +
 
 +
.works-hex .category {
 +
margin: 0;
 +
}
 +
}
 +
 
 +
@media all and (min-width:481px) and (max-width:1020px){
 +
 
 +
.works .category {
 +
margin: 0 1.5% 0;
 +
width: 25%;
 +
}
 +
 
 +
.four.works .category {
 +
margin: 0 1.5% 0;
 +
width: 18%;
 +
}
 +
}
 +
 
 +
@media all and (max-width:480px) {
 +
 
 +
.works .category {
 +
margin: 3% 1.5% 0;
 +
width: 25%;
 +
}
 +
 
 +
.four.works .category {
 +
margin: 3% 0.5% 0;
 +
width: 21%;
 +
}
 +
}
 +
 
 +
.works .thumbnail, .gallery .thumbnail {
 +
-webkit-border-radius: 5px;
 +
-moz-border-radius: 5px;
 +
border-radius: 5px;
 +
}
 +
 
 +
.wrapper {
 +
display: inline-block;
 +
position: relative;
 +
overflow: hidden;
 +
width: 100%;
 +
}
 +
 
 +
.works .wrapper {
 +
margin: 10% 0 17% 0;
 +
}
 +
 
 +
.works-hex .wrapper {
 +
margin: 17% 0;
 +
}
 +
 
 +
.works-hex .wrapper:after {
 +
padding-top: 116%; /*ratio*/
 +
display: block;
 +
content: '';
 +
}
 +
 
 +
.works-hex .hexagon {
 +
position: absolute;
 +
top: 0;
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bottom: 0;
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right: 0;
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.category-name {
 +
display: inline-block;
 +
margin-top: -9%;
 +
}
 +
 
 +
.win .category-name {
 +
margin-top: -12%;
 +
}
 +
 
 +
 
 +
/* ==========================================================================
 +
  About me
 +
  ========================================================================== */
 +
 
 +
.about-me {
 +
min-width: 280px;
 +
max-width: 826px;
 +
margin: 37px auto 45px;
 +
}
 +
 
 +
.twitter-stream {
 +
display: inline-block;
 +
width: 31%;
 +
margin: 0 0 0 4%;
 +
vertical-align: top;
 +
}
 +
 
 +
.about-me .about {
 +
width: 64%;
 +
display: inline-block;
 +
}
 +
 
 +
.square .about-me .about {
 +
width: 65%;
 +
display: inline-block;
 +
}
 +
 
 +
.square .twitter-stream {
 +
display: inline-block;
 +
width: 31%;
 +
margin: 0 0 0 3%;
 +
}
 +
 
 +
.tweets ul {
 +
list-style: none;
 +
margin: 0;
 +
padding: 0;
 +
}
 +
 
 +
.tweets img, .tweets .interact {
 +
display: none !important;
 +
visibility: hidden;
 +
}
 +
 
 +
 
 +
@media all and (max-width:800px) {
 +
 
 +
.square .about-me .about, .about-me .about {
 +
width: 100%;
 +
margin: 0 0 27px 0;
 +
}
 +
.square .about-me .twitter-stream, .about-me .twitter-stream {
 +
width: 100%;
 +
margin: 0 0 35px;
 +
display: inline-block;
 +
}
 +
}
 +
 
 +
 
 +
/* ==========================================================================
 +
  Blog & Single post
 +
  ========================================================================== */
 +
 
 +
.from-my-blog {
 +
min-width: 280px;
 +
max-width: 826px;
 +
margin: 0 auto;
 +
padding: 1px 0 90px;
 +
}
 +
 
 +
.blog-categories {
 +
width: 75%;
 +
min-width: 280px;
 +
max-width: 600px;
 +
margin: 30px auto 90px;
 +
}
 +
 
 +
.blog-excerpts {
 +
width: 66%;
 +
display: inline-block;
 +
}
 +
 
 +
 
 +
@media all and (max-width:800px) {
 +
.blog-excerpts {
 +
width: 100%;
 +
margin-right: 0;
 +
}
 +
}
 +
 
 +
.sidebar {
 +
width: 29%;
 +
margin: 14px 4% 0 0;
 +
display: inline-block;
 +
vertical-align: top;
 +
max-width: 240px;
 +
}
 +
 
 +
.blog-description {
 +
margin-top: -7px;
 +
}
 +
 
 +
.sidebar h2 {
 +
margin-left: -2%;
 +
width: 500px;
 +
position: relative;
 +
right: 137px;
 +
}
 +
 
 +
/*
 +
* Windows and mac font baseline are different,
 +
* so in order to get consistency I had to use a platform prefix
 +
*/
 +
 
 +
.win .sidebar h2 {
 +
margin-top: -18px;
 +
margin-bottom: 34px;
 +
}
 +
 
 +
@media all and (max-width:800px) {
 +
.sidebar {
 +
width: 100%;
 +
max-width: 800px;
 +
margin: 0;
 +
}
 +
 
 +
.blog-description {
 +
margin: 0 0 19px 0;
 +
display: inline-block;
 +
width: 75%;
 +
}
 +
 
 +
.sidebar h2 {
 +
display: inline-block;
 +
vertical-align: top;
 +
letter-spacing: -2px;
 +
margin: 10px 4% 0 0;
 +
 
 +
}
 +
 
 +
.win .sidebar h2 {
 +
margin: 2px 4% 0 0;
 +
}
 +
}
 +
 
 +
.blog-excerpts .post-excerpt, .blog-categories .post-excerpt {
 +
margin-bottom: 40px;
 +
}
 +
 
 +
.blog-excerpts .post-excerpt:last-child, .blog-categories .post-excerpt:last-child {
 +
margin-bottom: 0;
 +
}
 +
 
 +
.post {
 +
width: 85%;
 +
min-width: 280px;
 +
max-width: 600px;
 +
margin: 0 auto;
 +
padding: 45px 0 90px;
 +
}
 +
 
 +
@media all and (min-width:1021px) and (max-width:1400px) {
 +
 
 +
.post.two-column {
 +
max-width: 1060px;
 +
}
 +
}
 +
 
 +
@media all and (min-width:1401px) {
 +
 
 +
.post {
 +
max-width: 650px;
 +
}
 +
}
 +
 
 +
.categories-header {
 +
width: 75%;
 +
min-width: 280px;
 +
max-width: 800px;
 +
padding: 45px 0 0;
 +
margin: 0 auto;
 +
}
 +
 
 +
.video-embed {
 +
position: relative;
 +
padding: 30px 0 56.25%;
 +
margin: 0 0 1.5em;
 +
height: 0;
 +
overflow: hidden;
 +
}
 +
 
 +
.video-embed iframe,
 +
.video-embed object,
 +
.video-embed embed {
 +
position: absolute;
 +
top: 0;
 +
left: 0;
 +
width: 100%;
 +
height: 100%;
 +
}
 +
 
 +
.post figure {
 +
margin: 0 0 1.5em;
 +
}
 +
 
 +
.post img {
 +
width: 100%;
 +
}
 +
 
 +
 
 +
/* ==========================================================================
 +
  Gallery
 +
  ========================================================================== */
 +
 
 +
.gallery, .gallery-hex {
 +
width: 85%;
 +
min-width: 314px;
 +
}
 +
 
 +
.gallery-hex {
 +
max-width: 719px;
 +
margin: 70px auto 90px;
 +
}
 +
 
 +
.gallery {
 +
max-width: 800px;
 +
margin: 0 auto 90px;
 +
}
 +
 
 +
.gallery .wrapper, .gallery-hex .wrapper {
 +
display: inline-block;
 +
position: relative;
 +
overflow: hidden;
 +
 
 +
}
 +
 
 +
.gallery-hex .wrapper {
 +
width: 32%;
 +
margin: -9.3% 0.2% 0;
 +
}
 +
 
 +
.gallery .wrapper {
 +
width: 29%;
 +
min-width: 100px;
 +
margin: 2%  1.6%;
 +
}
 +
 
 +
.gallery .hexagon a {
 +
border: none;
 +
margin: 0;
 +
}
 +
 
 +
@media all and (max-width:800px) {
 +
 
 +
.gallery-hex {
 +
padding: 0 0 100px 1.9%;
 +
margin-top: 6%;
 +
}
 +
}
 +
 
 +
@media all and (max-width:320px) {
 +
 
 +
.gallery {
 +
margin-left: 5px;
 +
}
 +
 
 +
.gallery .wrapper {
 +
margin: 0 0 7px;
 +
}
 +
}
 +
 
 +
.gallery-hex .wrapper:after {
 +
padding-top: 116%;
 +
display: block;
 +
content: '';
 +
}
 +
 
 +
.gallery-hex .wrapper:hover {
 +
z-index: 100;
 +
}
 +
 
 +
.gallery-hex .hexagon {
 +
position: absolute;
 +
top: 0;
 +
bottom: 0;
 +
right: 0;
 +
left: 0;
 +
}
 +
 
 +
.gallery-hex .wrapper:nth-child(5n+4) {
 +
margin-left: 16.75%;
 +
}
 +
 
 +
.gallery-hex .hexagon a {
 +
border: none;
 +
margin: 0;
 +
}
 +
 
 +
 
 +
/* ==========================================================================
 +
  Single work
 +
  ========================================================================== */
 +
 
 +
.single-work {
 +
margin: 60px 0 90px;
 +
}
 +
 
 +
.description, .image {
 +
display: inline-block;
 +
vertical-align: top;
 +
}
 +
 
 +
.image {
 +
width: 67%;
 +
margin-left: 2em;
 +
}
 +
 
 +
.description {
 +
width: 29%;
 +
box-sizing: border-box;
 +
padding: 0 2em;
 +
margin-left: -4px;
 +
}
 +
 
 +
.image img {
 +
width: 100%;
 +
-webkit-border-radius: 3px;
 +
-moz-border-radius: 3px;
 +
border-radius: 3px;
 +
}
 +
 
 +
.image footer {
 +
margin-top: 20px;
 +
text-align: center;
 +
}
 +
 
 +
@media all and (max-width:1020px) {
 +
 
 +
.single-work {
 +
margin-top: 56px;
 +
}
 +
 
 +
.image {
 +
width: 100%;
 +
padding: 0;
 +
margin-left: 0;
 +
}
 +
 
 +
.description {
 +
width: 100%;
 +
padding: 0 2em;
 +
margin: 45px 0 0 0;
 +
}
 +
 
 +
.image img {
 +
-webkit-border-radius: 0px;
 +
-moz-border-radius: 0px;
 +
border-radius: 0px;
 +
}
 +
}
 +
 
 +
 
 +
@media all and (min-width:1601px) {
 +
 
 +
.image {
 +
width: 73%;
 +
}
 +
 
 +
.description {
 +
width: 20%;
 +
padding: 0 2%;
 +
}
 +
}
 +
 
 +
.gallery .wrapper a {
 +
border: none;
 +
margin: 0;
 +
padding: 0;
 +
}
 +
 
 +
/* ==========================================================================
 +
  Home button
 +
  ========================================================================== */
 +
 
 +
.home {
 +
position: absolute;
 +
top: 6px;
 +
left: 10px;
 +
font-size: 34px;
 +
}
 +
 
 +
.home a {
 +
margin: 0;
 +
border: none;
 +
 
 +
-webkit-transition: all 0.2s ease-out;
 +
  -moz-transition: all 0.2s ease-out;
 +
transition: all 0.2s ease-out;
 +
}
 +
 
 +
.home a:hover {
 +
-webkit-transition: all 0.2s ease-out;
 +
  -moz-transition: all 0.2s ease-out;
 +
transition: all 0.2s ease-out;
 +
}
 +
 
 +
 
 +
/* ==========================================================================
 +
  Footer
 +
  ========================================================================== */
 +
 
 +
.contact {
 +
margin: 45px 15px 15px;
 +
}
 +
 
 +
.copyright {
 +
margin: 0 15px 30px;
 +
}
 +
 
 +
 
 +
/* ==========================================================================
 +
  Helper classes
 +
  ========================================================================== */
 +
 
 +
/*
 +
* Image replacement
 +
*/
 +
 
 +
.ir {
 +
background-color: transparent;
 +
border: 0;
 +
overflow: hidden;
 +
/* IE 6/7 fallback */
 +
*text-indent: -9999px;
 +
}
 +
 
 +
.ir:before {
 +
content: "";
 +
display: block;
 +
width: 0;
 +
height: 100%;
 +
}
 +
 
 +
/*
 +
* Hide from both screenreaders and browsers: h5bp.com/u
 +
*/
 +
 
 +
.hidden {
 +
display: none !important;
 +
visibility: hidden;
 +
}
 +
 
 +
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Revision as of 01:21, 22 March 2014

iGEM San Diego

STATISTICS
Statistics that are quite relevant to the nature of this experiment

Toxicity of aflatoxin is 10 times that of hydrocyanic acid and 68 times of arsenic.

This disease is the third-leading cause of cancer death globally according to WHO (2008), with about 550,000–600,000 new cases each year.

This disease is the third-leading cause of cancer death globally according to WHO (2008), with about 550,000–600,000 new cases each year
ENGINEERING
E. Coli Capable of Extracelluar Secretion of Mycotoxin Detoxifying Enzymes
Microfungi that produce harmful mycotoxins flourish on improperly-stored nuts, grains, meat, and dairy. They especially thrive in developing countries, where the lack of advanced food storage and mycotoxin exposure causes 40% of the diseases. To lessen the problem, our team engineered E. coli strains using synthetic biology tools to produce chimeric mycotoxin-degrading fungal enzymes, Aflatoxin-Detoxifizyme (ADTZ) and Zearalenone Hydrolase (ZHD101), which are designed to be secreted to extra-cellular space by fusing with secretion signal peptides from alpha-amylase and beta-lactamase. In this study, we have successfully generated synthetic genetic materials to produce four chimeric mycotoxin-detoxifying enzymes. The levels of extracellular secretion is also characterized and analyzed. The project will allow a mass production of detoxification enzymes in cost effective way, preventing the squandering of harvested crops, and limiting mycotoxin-related diseases. Increased access to these proteins will have an immense commercial, industrial, agricultural, and health impact.

Journal

My Story | Mori

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Retrieved from "http://2014hs.igem.org/Team:TP_CC-SanDiego/Notebook/Journal/Test"