Team:CIDEB-UANL Mexico/labwork results

From 2014hs.igem.org

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<center><p><img width=380 height=260 src="https://static.igem.org/mediawiki/2014hs/4/4b/NhaS_%2B_pSB1C3_Ligation_.jpg"
<center><p><img width=380 height=260 src="https://static.igem.org/mediawiki/2014hs/4/4b/NhaS_%2B_pSB1C3_Ligation_.jpg"
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<p><font size="2"<b>Image 2. </b>NhaS first ligation with pSB1C3. Result of the transformation of the ligation between NhaS and pSB1C3. </p></font></center>
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<p><font size="2"><b>Image 2.</b>NhaS first ligation with pSB1C3. Result of the transformation of the ligation between NhaS and pSB1C3. </font></p></center>
<p><b><h2>Result of ligation BSMT1 opt. and pSB1C3 (Aroma module)</h2></b></p>
<p><b><h2>Result of ligation BSMT1 opt. and pSB1C3 (Aroma module)</h2></b></p>
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<center><p><img width=380 src="https://static.igem.org/mediawiki/2014hs/7/7c/Aroma_%2B_pSB1C3.jpg"
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<p><font size="2"><b>Image 3.</b>Colonies obtained from the transformation of the ligation BSMT1 opt and pSB1C3.  </font></p></center>
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<p><b><h2>UV Experimentation  </h2></b></p>
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<p>During 2 hours the four Petri dishes were exposed to UV irradiation. None of them showed any change. </p>
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<center><p><img width=380 src="https://static.igem.org/mediawiki/2014hs/7/75/First_UV_irradiation_Experiment.jpg"
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<p><font size="2"><b>Image 3.</b>Colonies obtained from the transformation of the ligation BSMT1 opt and pSB1C3.  </font></p></center>
<p><b><h2>Viability in salt (experiment 1)</h2></b></p>
<p><b><h2>Viability in salt (experiment 1)</h2></b></p>

Revision as of 04:27, 15 June 2014

Methods Notebook Results Interpretations Conclusions Future Plans
iGEM CIDEB 2014 - Project

Results

Ligation of NhaS and pSB1C3 (Capture module)

In the first transformation of the ligation of NhaS and pSB1C3 grew red (expressing RFP) and white bacteria.

Image 1. NhaS first ligation with pSB1C3. Result of the transformation of the ligation between NhaS and pSB1C3.

Image 2.NhaS first ligation with pSB1C3. Result of the transformation of the ligation between NhaS and pSB1C3.

Result of ligation BSMT1 opt. and pSB1C3 (Aroma module)

Image 3.Colonies obtained from the transformation of the ligation BSMT1 opt and pSB1C3.

UV Experimentation

During 2 hours the four Petri dishes were exposed to UV irradiation. None of them showed any change.

Image 3.Colonies obtained from the transformation of the ligation BSMT1 opt and pSB1C3.

Viability in salt (experiment 1)

Bacteria transformed with the capture plasmid were inoculated in Petri dishes with different concentrations of salt

IMG_0317

Image 1. All the 18 Petri dishes inoculated with NhaS Red in pSB1C3 of all the 9 used concentrations.


IMG_0317

Image 2. All the 18 Petri dishes inoculated with NhaS White in pSB1C3 of all the 9 used concentrations.


IMG_0317

Image 3. All the 18 Petri dishes inoculated with the Control bacteria of all the 9 used concentrations.

All the bacteria containing the NhaS in pSB1C3 (Red and White) survived to a 10% concentration of salt.

None of the control group lived in any concentration of salt.


IMG_0317

Image 4. Nine Petri dishes with the maximum concentration of salt (10%) used in this experiment. From up to bottom: NhaS Red in pSB1C3, NhaS White in pSB1C3 and the Control bacteria.


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