"
Team:Beijing HDFLS High/Project
From 2014hs.igem.org
(→Step) |
(→Step) |
||
| Line 31: | Line 31: | ||
=RESULTS AND CONCLUSIONS= | =RESULTS AND CONCLUSIONS= | ||
=Step= | =Step= | ||
| - | ==Step1 Transform and Miniprep== | + | ===Step1 Transform and Miniprep=== |
We get Part A plasmid from registry, and construct Part B plasmid. | We get Part A plasmid from registry, and construct Part B plasmid. | ||
| - | ==Step2 Digestion== | + | ===Step2 Digestion=== |
| - | ==Step3 Ligation== | + | ===Step3 Ligation=== |
| - | ==Step4 Digestion== | + | ===Step4 Digestion=== |
| - | ==Step5 Sequencing== | + | ===Step5 Sequencing=== |
Revision as of 13:41, 14 June 2014
Contents |
TITLE
The UV-responsing tandem generators for a wintergreen odor and protective melittin
ABSTRACT
The protective melittin generator is composed of two transcriptional devices: RecA (SOS) promoter (BBa_J22106) and synthesized melittin gene with enterokinase digested sequence. The protective generator takes as input extensive UV radiation and produces as output fusion melittin. Melittin can improve immunity and scavenging free radicals to alleviate the harm from electromagnetic radiation, such as blue light and UV.
We further construct a biological system that produces a wintergreen odor and protective melittin under extensive UV radiation. The biological system includes two generators: the UV-responsing wintergreen odor generator (BBa_K994000) and the protective melittin generator. It not only can warn people of intense UV irradiation by a wintergreen odor, but also produce protective melittin.
BACKGROUND
The electromagnetic spectrum, in order of increasing frequency and decreasing wavelength, can be divided, for practical engineering purposes, into radio waves, microwaves, infrared radiation, visible light, ultraviolet radiation, X-rays and gamma rays. The eyes of various organisms sense a relatively small range of frequencies of EMR called the visible spectrum or light. Higher frequencies (shorter wavelengths) correspond to proportionately more energy carried by each photon. Blue light is the most energetic portion of the visible light spectrum, it's less energetic than UV radiation but it also has the ability to penetrate into tissue and cause cellular damage.
Blue light is the hardest for the eye to focus on. We actually see a halo around bright blue light. Blue light scatters more inside the eyeball than other colors, producing more glare, more eyestrain and more fatigue. Intense blue light can cause damage to the retina, because blue is the hardest color for the retina to handle. The blue light induce that retinal produce free radicals. These radicals can lead to retinal pigment epithelial cell death. Then some light-sensitive cells lacks of nutrient, which results in visual impairment.
The old paint and song implies that the early ancient civilization was conscious of the possible virtues from bee stings.
Melittin is the main component (50~55%) of bee venom, which has multiple functions.
PART AND METHOD
RESULTS AND CONCLUSIONS
Step
Step1 Transform and Miniprep
We get Part A plasmid from registry, and construct Part B plasmid.
