Team:TP CC-SanDiego

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                    ><a href="/Talk:Team:KAIST-Korea">Discussion              </a></li>
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                    ><a href="/wiki/index.php?title=Team:KAIST-Korea&amp;action=edit">View source              </a></li>
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                    ><a href="/wiki/index.php?title=Team:KAIST-Korea&amp;action=history">History              </a></li>
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              <li style='color:#808080;cursor:default'>teams</li>
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top:108px; left:281px;
 +
width:71px; height:21px;
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}
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    <meta name="fragment" content="!"/>
+
#main_navigation
-
        <!-- DATA -->
+
{
-
<script type="text/javascript">
+
margin:0;
-
    var adData = {"topLabel":"<span class=\"smallMusa\">(Wix-Logo) </span>Create a <span class=\"smallLogo\">Wix</span> site!","topContent":"100s of templates<br />No coding needed<br /><span class=\"emphasis spacer\">Start now >></span>","footerLabel":"<div class=\"adFootBox\"><div class=\"siteBanner\" ><div class=\"siteBanner\"><div class=\"wrapper\"><div class=\"bigMusa\">(Wix Logo)</div><div class=\"txt shd\" style=\"color:#fff\">This site was created using </div> <div class=\"txt shd\"><a  href=\"http://www.wix.com?utm_campaign=vir_wixad_live\" style=\"color:#fff\"> WIX.com. </a></div> <div class=\"txt shd\" style=\"color:#fff\"> Create your own for FREE <span class=\"emphasis\"> >></span></div></div></div></div></div>","adUrl":"http://www.wix.com/lpviral/en900viral?utm_campaign=vir_wixad_live"};
+
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    var mobileAdData = {"footerLabel":"7c3dbd_67131d7bd570478689be752141d4e28a.jpg","adUrl":"http://www.wix.com/"};
+
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    var usersDomain = "https://users.wix.com//wix-users";
+
#main_navigation li
-
        </script>
+
{ float:left;
 +
list-style:none;
 +
font:12px Tahoma,Arial;
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}
 +
#main_navigation li a
 +
{ display:block;
 +
padding:5px 0px 0px 0px;
 +
text-decoration:none;
 +
border:none;
 +
color:#000000;
 +
width:auto;
 +
white-space:nowrap;}
 +
#main_navigation li a:hover
 +
{
 +
-moz-opacity:.70;filter:alpha(opacity=70);opacity:.70;
 +
}
 +
 +
#main_navigation li ul
 +
{ margin:0;
 +
padding:0;
 +
position:absolute;
 +
visibility:hidden;}
 +
 +
#main_navigation li ul li
 +
{ float:left;
 +
position:relative;
 +
left:5px;
 +
display:inline;
 +
}
 +
 +
#main_navigation li ul li a
 +
{ width:auto;
 +
padding:0px 0px 0px 0px;
 +
}
-
<script src="http://static.parastorage.com/services/wix-users/2.351.0/client/js/userApi_v2.js?cacheKiller=1"></script>
+
#main_navigation li ul li a:hover
-
<script src="http://static.parastorage.com/services/wix-users/2.351.0/user-api/user-api.min.js?cacheKiller=1"></script>
+
{ padding:5px 0px 0px 0px;
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}
 +
#subNav5 {
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position:relative;
 +
left:100px;
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display:inline;
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}
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#main_img1{
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border:0;
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width:70px; height:70px;
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background: transparent; -ms-filter: “progid:DXImageTransform.Microsoft.gradient(startColorstr=#00FFFFFF,endColorstr=#00FFFFFF)”; /* IE8 */
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filter: progid:DXImageTransform.Microsoft.gradient(startColorstr=#00FFFFFF,endColorstr=#00FFFFFF); /* IE6 &amp; 7 */ zoom: 1;
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border:0;
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width:70px; height:70px;
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background: transparent; -ms-filter: “progid:DXImageTransform.Microsoft.gradient(startColorstr=#00FFFFFF,endColorstr=#00FFFFFF)”; /* IE8 */
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filter: progid:DXImageTransform.Microsoft.gradient(startColorstr=#00FFFFFF,endColorstr=#00FFFFFF); /* IE6 &amp; 7 */ zoom: 1;
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 +
border:0;
 +
width:70px; height:70px;
 +
background: transparent; -ms-filter: “progid:DXImageTransform.Microsoft.gradient(startColorstr=#00FFFFFF,endColorstr=#00FFFFFF)”; /* IE8 */
 +
filter: progid:DXImageTransform.Microsoft.gradient(startColorstr=#00FFFFFF,endColorstr=#00FFFFFF); /* IE6 &amp; 7 */ zoom: 1;
 +
}#main_img4{
 +
border:0;
 +
width:70px; height:70px;
 +
background: transparent; -ms-filter: “progid:DXImageTransform.Microsoft.gradient(startColorstr=#00FFFFFF,endColorstr=#00FFFFFF)”; /* IE8 */
 +
filter: progid:DXImageTransform.Microsoft.gradient(startColorstr=#00FFFFFF,endColorstr=#00FFFFFF); /* IE6 &amp; 7 */ zoom: 1;
 +
}#main_img5{
 +
border:0;
 +
width:70px; height:70px;
 +
background: transparent; -ms-filter: “progid:DXImageTransform.Microsoft.gradient(startColorstr=#00FFFFFF,endColorstr=#00FFFFFF)”; /* IE8 */
 +
filter: progid:DXImageTransform.Microsoft.gradient(startColorstr=#00FFFFFF,endColorstr=#00FFFFFF); /* IE6 &amp; 7 */ zoom: 1;
 +
}#main_img6{
 +
border:0;
 +
width:70px; height:70px;
 +
background: transparent; -ms-filter: “progid:DXImageTransform.Microsoft.gradient(startColorstr=#00FFFFFF,endColorstr=#00FFFFFF)”; /* IE8 */
 +
filter: progid:DXImageTransform.Microsoft.gradient(startColorstr=#00FFFFFF,endColorstr=#00FFFFFF); /* IE6 &amp; 7 */ zoom: 1;
 +
}#main_img7{
 +
border:0;
 +
width:70px; height:70px;
 +
background: transparent; -ms-filter: “progid:DXImageTransform.Microsoft.gradient(startColorstr=#00FFFFFF,endColorstr=#00FFFFFF)”; /* IE8 */
 +
filter: progid:DXImageTransform.Microsoft.gradient(startColorstr=#00FFFFFF,endColorstr=#00FFFFFF); /* IE6 &amp; 7 */ zoom: 1;
 +
}
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<script type="text/javascript">
+
#menuall {
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    var userApi = UserApi.getInstance().init({
+
z-index:200;
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        "usersDomain":"http://users.wix.com//wix-users",
+
width:600px;
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        "corsEnabled":false,
+
position:relative;
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+
top:-25px;
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        "urlThatUserRedirectedFrom":"$"
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left:450px;
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+
}
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+
width:70px; height:70px;
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+
}
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</script>
+
 
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#kaistlogo{
 +
z-index:200;
 +
width:280px; height:77px;
 +
position:absolute;
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top:22px; left:66px;
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}
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#navigationbar{
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z-index:200;
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width:291px; heigth:7px;
 +
position:absolute;
 +
top:100px; left:63px;
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}
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 +
#subNav0{
 +
width:381px;height:80px;
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background-image:url('https://static.igem.org/mediawiki/2011/b/b6/Kaist2011_menu_menubar5_1.png');
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background-repeat:no-repeat;
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background-position:5px 7px;
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}
 +
 
 +
#subNav1{
 +
width:262px;height:80px;
 +
background-image:url('https://static.igem.org/mediawiki/2011/f/f0/Kaist2011_menu_menubar3.png');
 +
background-repeat:no-repeat;
 +
background-position:-22px 7px;
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}
 +
 
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#subNav2{
 +
width:192px;height:80px;
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background-image:url('https://static.igem.org/mediawiki/2011/6/61/Kaist2011_menu_menubar2.png');
 +
background-repeat:no-repeat;
 +
background-position:-23px 7px;
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}
 +
 
 +
#subNav3{
 +
width:262px;height:80px;
 +
background-image:url('https://static.igem.org/mediawiki/2011/f/f0/Kaist2011_menu_menubar3.png');
 +
background-repeat:no-repeat;
 +
background-position:-23px 7px;
 +
}
 +
 
 +
#subNav4{
 +
width:192px;height:80px;
 +
background-image:url('https://static.igem.org/mediawiki/2011/6/61/Kaist2011_menu_menubar2.png');
 +
background-repeat:no-repeat;
 +
background-position:-23px 7px;
 +
}
 +
 
 +
#subNav5{
 +
width:402px;height:80px;
 +
background-image:url('https://static.igem.org/mediawiki/2011/6/68/Kaist2011_menu_menubar5_2.png');
 +
background-repeat:no-repeat;
 +
background-position:-20px 7px;
 +
}
 +
 
 +
#menubody{
 +
padding-bottom:155px;
 +
}
 +
 
 +
</style>
 +
 
 +
<!--Start Glimmer Insertion-->
 +
<script type='text/javascript' src="/Team:KAIST-Korea/js/jquery-132min?action=raw"></script>
 +
<script type='text/javascript' src="/Team:KAIST-Korea/js/effects_core?action=raw"></script>
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<div id="menubody">
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+
<ul id="main_navigation">
 +
     <li id="trigger0"><a href=""><img id="main_img1" src="https://static.igem.org/mediawiki/2011/1/14/Kaist2011_menu_projects.png"></a>
 +
      <center><ul  class="subNavMenuItems" id="subNav0">
 +
          <li><a href="https://2011.igem.org/Team:KAIST-Korea/Projects/Overview"><img src="https://static.igem.org/mediawiki/2011/8/8c/Kaist2011_menu_overview.png"></a></li>
 +
  <li><a href="https://2011.igem.org/Team:KAIST-Korea/Projects/Modeling2"><img src="https://static.igem.org/mediawiki/2011/e/e1/Kaist2011_menu_modeling.png"></a></li>
 +
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 +
  <li><a href="https://2011.igem.org/Team:KAIST-Korea/Projects/Biobrick"><img src="https://static.igem.org/mediawiki/2011/9/99/Kaist2011_menu_biobrick.png"></a></li>
 +
  <li><a href="https://2011.igem.org/Team:KAIST-Korea/Projects/Future_Application"><img src="https://static.igem.org/mediawiki/2011/4/4e/Kaist2011_menu_future_application.png"></a></li>
 +
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 +
    </li>
 +
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<h2> <span class="mw-headline" id="Project_Description">Project Description</span></h2>
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<p><br />
 +
E. Casso is an E. coli system engineered into art. This system consists of two types of genetically modified E. coli: the random signal generating E. coli (the “Brush E. coli”) and the color generating E. coli (the “Paint E. coli”).
 +
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 +
The Brush E. coli possesses genes (lasI, luxI, rhlI, and cinI) that produce quorum in its chromosomal DNA. Each Brush E. coli will secrete quorum molecules randomly associated with one of four colors among red, cyan, yellow, and green by a modified Cre-loxP mechanism.
 +
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 +
The Paint E.coli possesses plasmid vectors that express one of four colors of fluorescent proteins in response to the type of quorum it receives from the Brush E.coli. The quorum sensing complex completed in the Brush will bind to the ribosome binding site (RBS) of the corresponding fluorescent gene.
 +
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 +
Using this system, we can create art on an LP plate with the cells' “random” contribution.
 +
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Revision as of 00:34, 13 June 2014

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Team:KAIST-Korea

From 2011.igem.org

Project Description

Kaist2011 home logo.png


E. Casso is an E. coli system engineered into art. This system consists of two types of genetically modified E. coli: the random signal generating E. coli (the “Brush E. coli”) and the color generating E. coli (the “Paint E. coli”).


The Brush E. coli possesses genes (lasI, luxI, rhlI, and cinI) that produce quorum in its chromosomal DNA. Each Brush E. coli will secrete quorum molecules randomly associated with one of four colors among red, cyan, yellow, and green by a modified Cre-loxP mechanism.


The Paint E.coli possesses plasmid vectors that express one of four colors of fluorescent proteins in response to the type of quorum it receives from the Brush E.coli. The quorum sensing complex completed in the Brush will bind to the ribosome binding site (RBS) of the corresponding fluorescent gene.


Using this system, we can create art on an LP plate with the cells' “random” contribution.

Retrieved from "https://2011.igem.org/Team:KAIST-Korea"

Retrieved from "http://2014hs.igem.org/Team:TP_CC-SanDiego"