Team:AUC TURKEY

From 2014hs.igem.org

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===Project===
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===Project Description===
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What are you working on this semester?
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This year we are working on the project 'BacteCooler 2.0'.
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In this project our aim is improve our last year project BacteCooler. Last year, in the beginning of the BacteCooler project we decided to use our project in real life as a product. This year we analyzed our project in terms of adaption BacteCooler to reality and found three main problems. Our solutions to these problems are;
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1) For safety of our project we should develop Kill-switch mechanisms. This mechanism activate on case of our bacteria contact with air. So we working on the promoters that sensible to molecules in the air.
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2) In our BacteCooler project our enzyme Urease catalyze the urea in the environment. After this catalyzation reaction NH3 come out and accumulate in the environment. This accumulation of NH3 causes the increase of pH and alkalinization of environment. After this increase of pH our bacteria will be dead. For the solve this problem we designed a circuit that activated with pH sensible promoter and then produce an acidic buffer protein that buffering this pH increase.
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3) Final and the biggest problem is over-night incubation time problem. For the solving this problem we should develop a system that secrete our urease enzyme quickly after the increase of temperature. We examined some of the iGEM projects and find some solutions that suit our project.
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This year for get a more efficient and exact results in our project we started to learn protein expressions experiment like Western-Blot, SDS-Page and ELISA. Also we working on modelling for get more information about how our project will work on practice.
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Finally we can say that in this iGEM HS 2014 adventure, we will try to solve this three problem with successful and much fun.
===Notebook===
===Notebook===

Revision as of 01:21, 8 March 2014


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have the following information on your wiki:
  • a team description
  • project description
  • safety information (did your team take a safety training course? were you supervised in the lab?)
  • team attribution (who did what part of your project?)
You may also wish to add other page such as:
  • lab notebook
  • sponsor information
  • other information
REMEMBER, keep all of your pages within your teams namespace.
Example: 2013hs.igem.org/Team:AUC_TURKEY/Our_Pets



You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
File:AUC TURKEY logo.png
200px

Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)

File:AUC TURKEY team.png
Your team picture
Team AUC_TURKEY


Official Team Profile

Contents

Team

Tell us about your team, your school!


Project Description

This year we are working on the project 'BacteCooler 2.0'. In this project our aim is improve our last year project BacteCooler. Last year, in the beginning of the BacteCooler project we decided to use our project in real life as a product. This year we analyzed our project in terms of adaption BacteCooler to reality and found three main problems. Our solutions to these problems are; 1) For safety of our project we should develop Kill-switch mechanisms. This mechanism activate on case of our bacteria contact with air. So we working on the promoters that sensible to molecules in the air. 2) In our BacteCooler project our enzyme Urease catalyze the urea in the environment. After this catalyzation reaction NH3 come out and accumulate in the environment. This accumulation of NH3 causes the increase of pH and alkalinization of environment. After this increase of pH our bacteria will be dead. For the solve this problem we designed a circuit that activated with pH sensible promoter and then produce an acidic buffer protein that buffering this pH increase. 3) Final and the biggest problem is over-night incubation time problem. For the solving this problem we should develop a system that secrete our urease enzyme quickly after the increase of temperature. We examined some of the iGEM projects and find some solutions that suit our project. This year for get a more efficient and exact results in our project we started to learn protein expressions experiment like Western-Blot, SDS-Page and ELISA. Also we working on modelling for get more information about how our project will work on practice. Finally we can say that in this iGEM HS 2014 adventure, we will try to solve this three problem with successful and much fun.

Notebook

Show us how you spent your days.


Results/Conclusions

What did you achieve over the course of your semester?


Safety

What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?


Attributions

Who worked on what?


Human Practices

What impact does/will your project have on the public?


Fun!

What was your favorite team snack?? Have a picture of your team mascot?


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