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Team:FHS Frederick MD/Sequencing
From 2014hs.igem.org
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{{:Team:FHS_Frederick_MD/Header}} | {{:Team:FHS_Frederick_MD/Header}} | ||
| + | |||
| + | =Sequencing= | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <pre> | ||
| + | CLUSTAL 2.1 multiple sequence alignment | ||
| + | |||
| + | |||
| + | LOV ------------GTTTCTTC------------------------------ 8 | ||
| + | 02-027-08-VF2_D11.ab1 NNNNNNNNNNNNNNNNNNNNANTANAAATAGNNNTATCACGAGGCAGAAT 50 | ||
| + | 02-027-06-VF2_C11.ab1 -NNNNNNNNNNNNNNNNNNNNNNAAAAATAGGCGTATCACGAGGCAGAAT 49 | ||
| + | |||
| + | |||
| + | LOV --------------------------------------------GAATTC 14 | ||
| + | 02-027-08-VF2_D11.ab1 TTCNGATAAAAAAAATCCTTAGCTTTCGCNANNGNNGATTTCTGGAATTC 100 | ||
| + | 02-027-06-VF2_C11.ab1 TTCAGATAAAAAAAATCCTTAGCTTTCGCNNAGGANGATTTCTGGAATTC 99 | ||
| + | ****** | ||
| + | |||
| + | LOV GCGGCCGCTTCTAGAGATGGCTTCTTTCCAATCTTTCGGTATCCCAGGTC 64 | ||
| + | 02-027-08-VF2_D11.ab1 GCGGCCGCTTCTAGAGATGGCTTCTTTCCAATCTTTCGGTATCCCAGGTC 150 | ||
| + | 02-027-06-VF2_C11.ab1 GCGGCCGCTTCTAGAGATGGCTTCTTTCCAATCTTTCGGTATCCCAGGTC 149 | ||
| + | ************************************************** | ||
| + | |||
| + | LOV AACTGGAAGTTATCAAAAAAGCTCTGGATCACGTTCGTGTTGGTGTTGTT 114 | ||
| + | 02-027-08-VF2_D11.ab1 AACTGGAAGTTATCAAAAAAGCTCTGGATCACGTTCGTGTTGGTGTTGTT 200 | ||
| + | 02-027-06-VF2_C11.ab1 AACTGGAAGTTATCAAAAAAGCTCTGGATCACGTTCGTGTTGGTGTTGTT 199 | ||
| + | ************************************************** | ||
| + | |||
| + | LOV ATCACTGATCCAGCTCTGGAAGATAACCCAATCGTTTACGTTAACCAAGG 164 | ||
| + | 02-027-08-VF2_D11.ab1 ATCACTGATCCAGCTCTGGAAGATAACCCAATCGTTTACGTTAACCAAGG 250 | ||
| + | 02-027-06-VF2_C11.ab1 ATCACTGATCCAGCTCTGGAAGATAACCCAATCGTTTACGTTAACCAAGG 249 | ||
| + | ************************************************** | ||
| + | |||
| + | LOV TTTCGTTCAAATGACTGGTTACGAAACTGAAGAAATCCTGGGTAAAAACG 214 | ||
| + | 02-027-08-VF2_D11.ab1 TTTCGTTCAAATGACTGGTTACGAAACTGAAGAAATCCTGGGTAAAAACG 300 | ||
| + | 02-027-06-VF2_C11.ab1 TTTCGTTCAAATGACTGGTTACGAAACTGAAGAAATCCTGGGTAAAAACG 299 | ||
| + | ************************************************** | ||
| + | |||
| + | LOV CTCGTTTCCTGCAAGGTAAACACACTGATCCAGCTGAAGTTGATAACATC 264 | ||
| + | 02-027-08-VF2_D11.ab1 CTCGTTTCCTGCAAGGTAAACACACTGATCCAGCTGAAGTTGATAACATC 350 | ||
| + | 02-027-06-VF2_C11.ab1 CTCGTTTCCTGCAAGGTAAACACACTGATCCAGCTGAAGTTGATAACATC 349 | ||
| + | ************************************************** | ||
| + | |||
| + | LOV CGTACTGCTCTGCAAAACAAAGAACCAGTTACTGTTCAAATCCAAAACTA 314 | ||
| + | 02-027-08-VF2_D11.ab1 CGTACTGCTCTGCAAAACAAAGAACCAGTTACTGTTCAAATCCAAAACTA 400 | ||
| + | 02-027-06-VF2_C11.ab1 CGTACTGCTCTGCAAAACAAAGAACCAGTTACTGTTCAAATCCAAAACTA 399 | ||
| + | ************************************************** | ||
| + | |||
| + | LOV CAAAAAAGATGGTACTATGTTCTGGAACGAACTGAACATCGATCCAATGG 364 | ||
| + | 02-027-08-VF2_D11.ab1 CAAAAAAGATGGTACTATGTTCTGGAACGAACTGAACATCGATCCAATGG 450 | ||
| + | 02-027-06-VF2_C11.ab1 CAAAAAAGATGGTACTATGTTCTGGAACGAACTGAACATCGATCCAATGG 449 | ||
| + | ************************************************** | ||
| + | |||
| + | LOV AAATCGAAGATAAAACTTACTTCGTTGGTATCCAAAACGATATCACTAAA 414 | ||
| + | 02-027-08-VF2_D11.ab1 AAATCGAAGATAAAACTTACTTCGTTGGTATCCAAAACGATATCACTAAA 500 | ||
| + | 02-027-06-VF2_C11.ab1 AAATCGAAGATAAAACTTACTTCGTTGGTATCCAAAACGATATCACTAAA 499 | ||
| + | ************************************************** | ||
| + | |||
| + | LOV CAAAAAGAATACGAAAAACTGCTGGAATAATACTAGTAGCGGCCGCTGCA 464 | ||
| + | 02-027-08-VF2_D11.ab1 CAAAAAGAATACGAAAAACTGCTGGAATAATACTAGTAGCGGCCGCTGCA 550 | ||
| + | 02-027-06-VF2_C11.ab1 CAAAAAGAATACGAAAAACTGCTGGAATAATACTAGTAGCGGCCGCTGCA 549 | ||
| + | ************************************************** | ||
| + | |||
| + | LOV G---------GAAG---------------------------------AAA 472 | ||
| + | 02-027-08-VF2_D11.ab1 GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAA 600 | ||
| + | 02-027-06-VF2_C11.ab1 GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAA 599 | ||
| + | * *** *** | ||
| + | |||
| + | LOV C------------------------------------------------- 473 | ||
| + | 02-027-08-VF2_D11.ab1 CCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCT 650 | ||
| + | 02-027-06-VF2_C11.ab1 CCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCT 649 | ||
| + | * | ||
| + | |||
| + | LOV -------------------------------------------------- | ||
| + | 02-027-08-VF2_D11.ab1 GCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAA-GGCG 699 | ||
| + | 02-027-06-VF2_C11.ab1 GCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCNAAAGGCG 699 | ||
| + | |||
| + | |||
| + | LOV -------------------------------------------------- | ||
| + | 02-027-08-VF2_D11.ab1 GTAATACGGTTATCCACAGAATCAGGGGATAACGCANGAAAGAACATGTG 749 | ||
| + | 02-027-06-VF2_C11.ab1 GTAATACGGTTATCCACAGAATCAGGGGATAACGCANGAAAGAACATGTG 749 | ||
| + | |||
| + | |||
| + | LOV -------------------------------------------------- | ||
| + | 02-027-08-VF2_D11.ab1 AGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGNCGCGTTGCTGG 799 | ||
| + | 02-027-06-VF2_C11.ab1 AGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG 799 | ||
| + | |||
| + | |||
| + | LOV -------------------------------------------------- | ||
| + | 02-027-08-VF2_D11.ab1 CGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 849 | ||
| + | 02-027-06-VF2_C11.ab1 CGTTTTTCCACANGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 849 | ||
| + | |||
| + | |||
| + | LOV -------------------------------------------------- | ||
| + | 02-027-08-VF2_D11.ab1 TCAAGTCNNAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTT 899 | ||
| + | 02-027-06-VF2_C11.ab1 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCANGCGTT 899 | ||
| + | |||
| + | |||
| + | LOV -------------------------------------------------- | ||
| + | 02-027-08-VF2_D11.ab1 TCCCCCTGGAAAGCTCCCTCGTGCGCTCTCCTGTTCCNACCCTGCCGCTT 949 | ||
| + | 02-027-06-VF2_C11.ab1 TCCCCCTGGAAAGCTCCCTCGTGCGCTCTCCTGTTCCNACCCTGCCGCTT 949 | ||
| + | |||
| + | |||
| + | LOV -------------------------------------------------- | ||
| + | 02-027-08-VF2_D11.ab1 ACCGGATACCTGTCCGCCTTTCTCCCTTCGGGNAANCGTGGCGCTTTCTC 999 | ||
| + | 02-027-06-VF2_C11.ab1 ACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA-GCGTGGCNCTTTCTC 998 | ||
| + | |||
| + | |||
| + | LOV -------------------------------------------------- | ||
| + | 02-027-08-VF2_D11.ab1 ATAGCTCACGNNTGTAGGNATCTCAGTTCGGNGNAGGTCGTTCGCTNCNA 1049 | ||
| + | 02-027-06-VF2_C11.ab1 ATAGCTCACGCT-GTNNGTATCTCANTTCNGNGNNAGNCGTTCGCNNCNA 1047 | ||
| + | |||
| + | |||
| + | LOV -------------------------------------------------- | ||
| + | 02-027-08-VF2_D11.ab1 GCTGGG-CTNNNGNGCNCGAACCCCCGTTCANNCCGACGNCTGCNCCTTA 1098 | ||
| + | 02-027-06-VF2_C11.ab1 NCTNGGGCTNNNGNNCANNAANNNNC------------------------ 1073 | ||
| + | |||
| + | |||
| + | LOV -------------------------------------------------- | ||
| + | 02-027-08-VF2_D11.ab1 TCCNGNNANNTATNGTCTTGANTCCAACCCGGGTAANAANNNNAANTTNA 1148 | ||
| + | 02-027-06-VF2_C11.ab1 -------------------------------------------------- | ||
| + | |||
| + | |||
| + | LOV -------- | ||
| + | 02-027-08-VF2_D11.ab1 NNNNCNNN 1156 | ||
| + | 02-027-06-VF2_C11.ab1 -------- | ||
| + | </pre> | ||
Revision as of 22:04, 19 June 2014
Sequencing
CLUSTAL 2.1 multiple sequence alignment
LOV ------------GTTTCTTC------------------------------ 8
02-027-08-VF2_D11.ab1 NNNNNNNNNNNNNNNNNNNNANTANAAATAGNNNTATCACGAGGCAGAAT 50
02-027-06-VF2_C11.ab1 -NNNNNNNNNNNNNNNNNNNNNNAAAAATAGGCGTATCACGAGGCAGAAT 49
LOV --------------------------------------------GAATTC 14
02-027-08-VF2_D11.ab1 TTCNGATAAAAAAAATCCTTAGCTTTCGCNANNGNNGATTTCTGGAATTC 100
02-027-06-VF2_C11.ab1 TTCAGATAAAAAAAATCCTTAGCTTTCGCNNAGGANGATTTCTGGAATTC 99
******
LOV GCGGCCGCTTCTAGAGATGGCTTCTTTCCAATCTTTCGGTATCCCAGGTC 64
02-027-08-VF2_D11.ab1 GCGGCCGCTTCTAGAGATGGCTTCTTTCCAATCTTTCGGTATCCCAGGTC 150
02-027-06-VF2_C11.ab1 GCGGCCGCTTCTAGAGATGGCTTCTTTCCAATCTTTCGGTATCCCAGGTC 149
**************************************************
LOV AACTGGAAGTTATCAAAAAAGCTCTGGATCACGTTCGTGTTGGTGTTGTT 114
02-027-08-VF2_D11.ab1 AACTGGAAGTTATCAAAAAAGCTCTGGATCACGTTCGTGTTGGTGTTGTT 200
02-027-06-VF2_C11.ab1 AACTGGAAGTTATCAAAAAAGCTCTGGATCACGTTCGTGTTGGTGTTGTT 199
**************************************************
LOV ATCACTGATCCAGCTCTGGAAGATAACCCAATCGTTTACGTTAACCAAGG 164
02-027-08-VF2_D11.ab1 ATCACTGATCCAGCTCTGGAAGATAACCCAATCGTTTACGTTAACCAAGG 250
02-027-06-VF2_C11.ab1 ATCACTGATCCAGCTCTGGAAGATAACCCAATCGTTTACGTTAACCAAGG 249
**************************************************
LOV TTTCGTTCAAATGACTGGTTACGAAACTGAAGAAATCCTGGGTAAAAACG 214
02-027-08-VF2_D11.ab1 TTTCGTTCAAATGACTGGTTACGAAACTGAAGAAATCCTGGGTAAAAACG 300
02-027-06-VF2_C11.ab1 TTTCGTTCAAATGACTGGTTACGAAACTGAAGAAATCCTGGGTAAAAACG 299
**************************************************
LOV CTCGTTTCCTGCAAGGTAAACACACTGATCCAGCTGAAGTTGATAACATC 264
02-027-08-VF2_D11.ab1 CTCGTTTCCTGCAAGGTAAACACACTGATCCAGCTGAAGTTGATAACATC 350
02-027-06-VF2_C11.ab1 CTCGTTTCCTGCAAGGTAAACACACTGATCCAGCTGAAGTTGATAACATC 349
**************************************************
LOV CGTACTGCTCTGCAAAACAAAGAACCAGTTACTGTTCAAATCCAAAACTA 314
02-027-08-VF2_D11.ab1 CGTACTGCTCTGCAAAACAAAGAACCAGTTACTGTTCAAATCCAAAACTA 400
02-027-06-VF2_C11.ab1 CGTACTGCTCTGCAAAACAAAGAACCAGTTACTGTTCAAATCCAAAACTA 399
**************************************************
LOV CAAAAAAGATGGTACTATGTTCTGGAACGAACTGAACATCGATCCAATGG 364
02-027-08-VF2_D11.ab1 CAAAAAAGATGGTACTATGTTCTGGAACGAACTGAACATCGATCCAATGG 450
02-027-06-VF2_C11.ab1 CAAAAAAGATGGTACTATGTTCTGGAACGAACTGAACATCGATCCAATGG 449
**************************************************
LOV AAATCGAAGATAAAACTTACTTCGTTGGTATCCAAAACGATATCACTAAA 414
02-027-08-VF2_D11.ab1 AAATCGAAGATAAAACTTACTTCGTTGGTATCCAAAACGATATCACTAAA 500
02-027-06-VF2_C11.ab1 AAATCGAAGATAAAACTTACTTCGTTGGTATCCAAAACGATATCACTAAA 499
**************************************************
LOV CAAAAAGAATACGAAAAACTGCTGGAATAATACTAGTAGCGGCCGCTGCA 464
02-027-08-VF2_D11.ab1 CAAAAAGAATACGAAAAACTGCTGGAATAATACTAGTAGCGGCCGCTGCA 550
02-027-06-VF2_C11.ab1 CAAAAAGAATACGAAAAACTGCTGGAATAATACTAGTAGCGGCCGCTGCA 549
**************************************************
LOV G---------GAAG---------------------------------AAA 472
02-027-08-VF2_D11.ab1 GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAA 600
02-027-06-VF2_C11.ab1 GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAA 599
* *** ***
LOV C------------------------------------------------- 473
02-027-08-VF2_D11.ab1 CCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCT 650
02-027-06-VF2_C11.ab1 CCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCT 649
*
LOV --------------------------------------------------
02-027-08-VF2_D11.ab1 GCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAA-GGCG 699
02-027-06-VF2_C11.ab1 GCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCNAAAGGCG 699
LOV --------------------------------------------------
02-027-08-VF2_D11.ab1 GTAATACGGTTATCCACAGAATCAGGGGATAACGCANGAAAGAACATGTG 749
02-027-06-VF2_C11.ab1 GTAATACGGTTATCCACAGAATCAGGGGATAACGCANGAAAGAACATGTG 749
LOV --------------------------------------------------
02-027-08-VF2_D11.ab1 AGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGNCGCGTTGCTGG 799
02-027-06-VF2_C11.ab1 AGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG 799
LOV --------------------------------------------------
02-027-08-VF2_D11.ab1 CGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 849
02-027-06-VF2_C11.ab1 CGTTTTTCCACANGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 849
LOV --------------------------------------------------
02-027-08-VF2_D11.ab1 TCAAGTCNNAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTT 899
02-027-06-VF2_C11.ab1 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCANGCGTT 899
LOV --------------------------------------------------
02-027-08-VF2_D11.ab1 TCCCCCTGGAAAGCTCCCTCGTGCGCTCTCCTGTTCCNACCCTGCCGCTT 949
02-027-06-VF2_C11.ab1 TCCCCCTGGAAAGCTCCCTCGTGCGCTCTCCTGTTCCNACCCTGCCGCTT 949
LOV --------------------------------------------------
02-027-08-VF2_D11.ab1 ACCGGATACCTGTCCGCCTTTCTCCCTTCGGGNAANCGTGGCGCTTTCTC 999
02-027-06-VF2_C11.ab1 ACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA-GCGTGGCNCTTTCTC 998
LOV --------------------------------------------------
02-027-08-VF2_D11.ab1 ATAGCTCACGNNTGTAGGNATCTCAGTTCGGNGNAGGTCGTTCGCTNCNA 1049
02-027-06-VF2_C11.ab1 ATAGCTCACGCT-GTNNGTATCTCANTTCNGNGNNAGNCGTTCGCNNCNA 1047
LOV --------------------------------------------------
02-027-08-VF2_D11.ab1 GCTGGG-CTNNNGNGCNCGAACCCCCGTTCANNCCGACGNCTGCNCCTTA 1098
02-027-06-VF2_C11.ab1 NCTNGGGCTNNNGNNCANNAANNNNC------------------------ 1073
LOV --------------------------------------------------
02-027-08-VF2_D11.ab1 TCCNGNNANNTATNGTCTTGANTCCAACCCGGGTAANAANNNNAANTTNA 1148
02-027-06-VF2_C11.ab1 --------------------------------------------------
LOV --------
02-027-08-VF2_D11.ab1 NNNNCNNN 1156
02-027-06-VF2_C11.ab1 --------
