Team:CIDEB-UANL Mexico/labwork results

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Revision as of 04:34, 15 June 2014

Methods Notebook Results Interpretations Conclusions Future Plans

iGEM CIDEB 2014 - Project

Results

Ligation of NhaS and pSB1C3 (Capture module)

In the first transformation of the ligation of NhaS and pSB1C3 grew red (expressing RFP) and white bacteria.

Image 1. NhaS first ligation with pSB1C3. Result of the transformation of the ligation between NhaS and pSB1C3.

Image 2.NhaS first ligation with pSB1C3. Result of the transformation of the ligation between NhaS and pSB1C3.

Result of ligation BSMT1 opt. and pSB1C3 (Aroma module)

Image 3.Colonies obtained from the transformation of the ligation BSMT1 opt and pSB1C3.

UV Experimentation

During 2 hours the four Petri dishes were exposed to UV irradiation. None of them showed any change.

Image 4.Petri Dish inoculated with NhaS in pSB1C3 exposed to UV irradiation. Before, no time being exposed (left) and after 2 hours of being exposed (right).

Repetition UV Experimentation

Two Petri dishes inoculated by streak: One with NhaS Red and the other with NhaS White. Both exposed during 30 minutes to UV irradiation and none of them showed any change.

With no time exposed to UV irradiation:

Image 5.Nhas in pSB1C3 before being exposed to UV irradiation.

After 30 minutes:

Image 5.Nhas in pSB1C3 before being exposed to UV irradiation.

Viability in salt (experiment 1)

Bacteria transformed with the capture plasmid were inoculated in Petri dishes with different concentrations of salt

Image 6. All the 18 Petri dishes inoculated with NhaS Red in pSB1C3 of all the 9 used concentrations.

Image 7. All the 18 Petri dishes inoculated with NhaS White in pSB1C3 of all the 9 used concentrations.

Image 8. All the 18 Petri dishes inoculated with the Control bacteria of all the 9 used concentrations.

All the bacteria containing the NhaS in pSB1C3 (Red and White) survived to a 10% concentration of salt.

None of the control group lived in any concentration of salt.

Image 10. Nine Petri dishes with the maximum concentration of salt (10%) used in this experiment. From up to bottom: NhaS Red in pSB1C3, NhaS White in pSB1C3 and the Control bacteria.

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iGEM CIDEB 2014 - Footer

iGEM CIDEB UANL 2014. CENTRO DE INVESTIGACIÓN Y DESARROLLO DE EDUCACIÓN BILINGÜE. UANL

@@ Line 362: / Line 362: @@
 <center><p><img width=500 src="https://static.igem.org/mediawiki/2014hs/1/18/Before_NhaS_Video.jpg"
+align=center hspace=12></p>
+<p><font size="2"><b>Image 5.</b>Nhas in pSB1C3 before being exposed to UV irradiation.    </font></p></center>
+<p>After 30 minutes:
+</p>
+<center><p><img width=500 src="https://static.igem.org/mediawiki/2014hs/a/a6/After_NhaS_Video_.jpg"
 align=center hspace=12></p>
 <p><font size="2"><b>Image 5.</b>Nhas in pSB1C3 before being exposed to UV irradiation.    </font></p></center>
@@ Line 372: / Line 379: @@
 <center><p><img width=380 height=260 src="https://static.igem.org/mediawiki/2014hs/e/e2/Rojas_NhaS_experiment_1.jpg"
 align=center hspace=12 alt="IMG_0317"></p>
-<p><font size="2"><b>Image 1. </b>All the 18 Petri dishes inoculated with NhaS Red in pSB1C3 of all the 9 used concentrations.</font></p></center>
+<p><font size="2"><b>Image 6. </b>All the 18 Petri dishes inoculated with NhaS Red in pSB1C3 of all the 9 used concentrations.</font></p></center>
 <br>
 <center><p><img width=380 height=260 src="https://static.igem.org/mediawiki/2014hs/2/28/Blancas_NhaS_experimen_1.jpg"
 align=center hspace=12 alt="IMG_0317"></p>
-<p><font size="2"><b>Image 2. </b>All the 18 Petri dishes inoculated with NhaS White in pSB1C3 of all the 9 used concentrations.</font></p></center>
+<p><font size="2"><b>Image 7. </b>All the 18 Petri dishes inoculated with NhaS White in pSB1C3 of all the 9 used concentrations.</font></p></center>
 <br>
 <center><p><img width=380 height=260 src="https://static.igem.org/mediawiki/2014hs/1/12/Control_Expriment_1_NhaS_.jpg"
 align=center hspace=12 alt="IMG_0317"></p>
-<p><font size="2"><b>Image 3. </b>All the 18 Petri dishes inoculated with the Control bacteria of all the 9 used concentrations.</font></p></center>
+<p><font size="2"><b>Image 8. </b>All the 18 Petri dishes inoculated with the Control bacteria of all the 9 used concentrations.</font></p></center>
 <p>All the bacteria containing the NhaS in pSB1C3 (Red and White) survived to a 10% concentration of salt.</p>
 <p>None of the control group lived in any concentration of salt.</p>
@@ Line 389: / Line 396: @@
 <center><p><img width=262 height=403 src="https://static.igem.org/mediawiki/2014hs/2/28/Experiment_1_Maximum_concentration_of_salt..jpg"
 align=center hspace=12 alt="IMG_0317"></p>
-<p><font size="2"><b>Image 4. </b>Nine Petri dishes with the maximum concentration of salt (10%) used in this experiment. From up to bottom: NhaS Red in pSB1C3, NhaS White in pSB1C3 and the Control bacteria.</font></p></center>
+<p><font size="2"><b>Image 10. </b>Nine Petri dishes with the maximum concentration of salt (10%) used in this experiment. From up to bottom: NhaS Red in pSB1C3, NhaS White in pSB1C3 and the Control bacteria.</font></p></center>
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