|
|
| Line 1,154: |
Line 1,154: |
| | <div id="myleftbox" class="smallbox"> | | <div id="myleftbox" class="smallbox"> |
| | <h1> Fun </h1> | | <h1> Fun </h1> |
| - | <center><img src="https://static.igem.org/mediawiki/2014hs/6/67/We_Like_Synthetic_Biology.png" width="600"></center><br>
| + | <br> |
| | <p>Who says that science can't be fun?</p> | | <p>Who says that science can't be fun?</p> |
| | <br> | | <br> |
| - | <img src="https://static.igem.org/mediawiki/2014hs/0/05/3185999.gif">
| + | <br> |
| - | <br><br>
| + | <b>Cyano Machine</b> |
| - | <b>Glycolysis: A Poem</b> | + | <br> To the tune of Yellow Submarine |
| - | <br> by Alec Adams <br><br> | + | <br> by Muriel Eaton (with help from my friends Lennon and McCartney) <br><br> |
| - | | + | |
| - | Glycolysis always just starts with glucose, <br>
| + | |
| - | In the cytosol, now glucose where do you go? <br>
| + | |
| - | It could fly away, to the extracellular space, <br>
| + | |
| - | But it gets phosphorylated by hexokinase, <br>
| + | |
| - | It costs ATP, to make G-6-P, <br>
| + | |
| - | But now it's trapped in the cell, and can proceed, <br>
| + | |
| - | If there's plenty of ATP, and insulin, <br>
| + | |
| - | Then we've got the conditions to make glycogen, <br>
| + | |
| - | Product inhibition by HK's alright, <br>
| + | |
| - | But we need ATP: we isomerize... <br>
| + | |
| - | To fructose-6-phosphate, are you listening yet? <br>
| + | |
| - | Cause PFK's the rate limiting step, <br>
| + | |
| - | Inhibited yes, by allosterically fitting, <br>
| + | |
| - | And changing the enzyme, it's finicky, this could be... <br>
| + | |
| - | From PCr, ATP and apparently, <br>
| + | |
| - | Citrate and H plus also works merrily, <br>
| + | |
| - | To enhance, the rate take a stance, <br>
| + | |
| - | With ADP, AMP, Pi and dance, <br>
| + | |
| - | One last time, ATP is consumed, <br>
| + | |
| - | To make fructose-1-6-diphosphate, it's all true, <br>
| + | |
| - | That 6 carbon has to go, all the way, <br>
| + | |
| - | To 3 carbon backbones, by aldolase, <br>
| + | |
| - | Making dihydroxyacetone, phosphate, <br>
| + | |
| - | And to the other 3-C backbone it can go, <br>
| + | |
| - | And isomerize, <br><br>
| + | |
| - | To 3-phosphoglyceraldehyde, <br>
| + | |
| - | With Pi, the enzyme, is a dehydrogenase , <br>
| + | |
| - | It always makes NADH, and that's all it takes, <br>
| + | |
| - | But wait, we added a phosphate to the substrate, <br>
| + | |
| - | 1-3-diphosphoglycerate, <br><br>
| + | |
| - | | + | |
| - | We find ways with a kinase for ATP making, <br>
| + | |
| - | The next enzyme catalyzes substrate phosphorylating, <br>
| + | |
| - | are we even with ATP? you can believe it, <br>
| + | |
| - | Make 3-phosphoglycerate and kinase does speed it, <br>
| + | |
| - | Then we do make 2-phosphoglycerate with a mutase, <br>
| + | |
| - | Which will enolize to phosphoenopyruvate, <br>
| + | |
| - | Now for the "pay-off" as it's often called, <br>
| + | |
| - | We get 2 ATP cause we double it alllllll.... <br>
| + | |
| - | Of it with pyruvate kinase we control, <br>
| + | |
| - | The reaction it happens cause PEP makes it go, <br>
| + | |
| - | But it goes slow with PCr and citrate, <br>
| + | |
| - | 2 net ATP in the end and that's great! <br><br>
| + | |
| | | | |
| - | We've gone a long way, and by coming this far, <br>
| + | Under Construction. |
| - | We make pyruvate and now here we are, <br>
| + | |
| - | Are aerobic or anaerobic? <br>
| + | |
| - | We can be both, whichever you pick, <br>
| + | |
| - | At steady state or low intensity, <br>
| + | |
| - | We predominantly do OP-ETC, <br>
| + | |
| - | If we need energy fast we can run, <br>
| + | |
| - | To LDH, and fermentation, <br>
| + | |
| - | H+ is bad, but lactate is fuel, <br>
| + | |
| - | It's used in the liver and muscle tissue, <br>
| + | |
| - | It's a reduction reaction because, <br>
| + | |
| - | We oxidize NADH and H+,<br>
| + | |
| - | To cycle it back, as a cofactor, <br>
| + | |
| - | For g-3-p-d, H know why it matters, <br>
| + | |
| - | Hope you could follow it, that's all there is, <br>
| + | |
| - | To explaining the basics of glycolysis! <br><br>
| + | |
| | | | |
| | </div> | | </div> |
"
