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Revision as of 14:22, 14 June 2014 (view source) Hongmei (Talk | contribs) (→Step2 Digestion) ← Older edit		Revision as of 14:24, 14 June 2014 (view source) Hongmei (Talk | contribs) (→Step1 Transformation and Miniprep) Newer edit →
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	===Step1 Transformation and Miniprep===		===Step1 Transformation and Miniprep===
	We get Part A plasmid from registry, and construct Part B plasmid.		We get Part A plasmid from registry, and construct Part B plasmid.
		+	[[File:step1.png|center|600px]]
	===Step2 Digestion===		===Step2 Digestion===

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Revision as of 14:24, 14 June 2014

Contents 1 TITLE 2 ABSTRACT 3 BACKGROUND 3.1 Blue light from computer induces eye retina producing free radical 3.2 Melittin has funcition of scavenging free radical 4 PART AND METHOD 5 RESULTS AND CONCLUSIONS 6 Step 6.1 Step1 Transformation and Miniprep 6.2 Step2 Digestion 6.3 Step3 Ligation 6.4 Step4 Digestion 6.5 Step5 Sequencing

TITLE

The UV-responsing tandem generators for a wintergreen odor and protective melittin

ABSTRACT

The protective melittin generator is composed of two transcriptional devices: RecA (SOS) promoter (BBa_J22106) and synthesized melittin gene with enterokinase digested sequence. The protective generator takes as input extensive UV radiation and produces as output fusion melittin. Melittin can improve immunity and scavenging free radicals to alleviate the harm from electromagnetic radiation, such as blue light and UV.

We further construct a biological system that produces a wintergreen odor and protective melittin under extensive UV radiation. The biological system includes two generators: the UV-responsing wintergreen odor generator (BBa_K994000) and the protective melittin generator. It not only can warn people of intense UV irradiation by a wintergreen odor, but also produce protective melittin.

BACKGROUND

Blue light from computer induces eye retina producing free radical

The electromagnetic spectrum, in order of increasing frequency and decreasing wavelength, can be divided, for practical engineering purposes, into radio waves, microwaves, infrared radiation, visible light, ultraviolet radiation, X-rays and gamma rays. The eyes of various organisms sense a relatively small range of frequencies of EMR called the visible spectrum or light. Higher frequencies (shorter wavelengths) correspond to proportionately more energy carried by each photon. Blue light is the most energetic portion of the visible light spectrum, it's less energetic than UV radiation but it also has the ability to penetrate into tissue and cause cellular damage.

Blue light is the hardest for the eye to focus on. We actually see a halo around bright blue light. Blue light scatters more inside the eyeball than other colors, producing more glare, more eyestrain and more fatigue. Intense blue light can cause damage to the retina, because blue is the hardest color for the retina to handle. The blue light induce that retinal produce free radicals. These radicals can lead to retinal pigment epithelial cell death. Then some light-sensitive cells lacks of nutrient, which results in visual impairment.

Melittin has funcition of scavenging free radical

The old paint and song implies that the early ancient civilization was conscious of the possible virtues from bee stings.

Melittin is the main component (50~55%) of bee venom, which has multiple functions.

PART AND METHOD

RESULTS AND CONCLUSIONS

Step

Step1 Transformation and Miniprep

We get Part A plasmid from registry, and construct Part B plasmid.

Step2 Digestion

Step3 Ligation

Step4 Digestion

Step5 Sequencing