Team:PEA Exeter NH/Notebook Raw

From 2014hs.igem.org

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Part number and plasmid: BBa_J04500, BBa_J04650, pSB1C3</br>
Part number and plasmid: BBa_J04500, BBa_J04650, pSB1C3</br>
Results: TBA</br>
Results: TBA</br>
-
 
</br>
</br>
</br>
</br>
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Mini prep: BBa_K518010_pSB1C3, BBa_J61051_pSB1C3</br>
Mini prep: BBa_K518010_pSB1C3, BBa_J61051_pSB1C3</br>
Species: E. Coli</br>
Species: E. Coli</br>
 +
Results: Worked Well </br>
</br>
</br>
</br>
</br>
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Results: TBA</br>
Results: TBA</br>
Tube 1- BBa_J61051_BBa_K118021_pSB1C3</br>
Tube 1- BBa_J61051_BBa_K118021_pSB1C3</br>
 +
</br>
 +
</br>
 +
MINIPREP:</br>
 +
Names: Christina S.</br>
 +
Date: May 22nd, 2014</br>
 +
Cultures for Miniprep: BBa_K518010_pSB1C3 (SOS), BBa_J61051_pSB1C3 (NAP), BBa_K118021 _pSB1C3 (CAT), BBa_K592009_pSB1C3 (BLUE) [samples prepped in duplicate]</br>
 +
Results: TBA</br>
 +
</br>
 +
</br>
 +
MEASURING DNA CONCENTRATION FROM MINIPREP</br>
 +
Names: Alex S, Christina S</br>
 +
Date: May 23rd, 2014</br>
 +
Samples from Miniprep</br>
 +
Results: DNA Concentrations</br>
 +
NAP1: 26.25 ng/ul</br>
 +
NAP2: 33.50 ng/ul</br>
 +
CAT1: 39.50 ng/ul</br>
 +
CAT2: 21.00 ng/ul</br>
 +
SOS1: 29.75 ng/ul</br>
 +
SOS2 (from mini prep on 5.21.14): 30.50 ng/ul</br>
 +
BLUE1: 70.25 ng/ul</br>
 +
BLUE2: 20.50 ng/ul</br>
 +
</br>
 +
</br>
 +
RESTRICITION DIGEST</br>
 +
Names: Christina S, Reginald L., Alex S.</br>
 +
Date: May 23rd, 2014</br>
 +
Samples from Miniprep</br>
 +
</br>
 +
</br>
 +
GEL ELECTROPHORESIS ON DIGEST PRODUCT</br>
 +
Names: Christina S</br>
 +
Date: May 25th, 2014</br>
 +
Samples from restriction digest</br>
 +
</br>
 +
</br>
 +
<img src=http://i.imgur.com/mR2AlIC.jpg?1></p>
 +
<p style="clear: both;">
 +
</br>
 +
</br>
 +
Loading Order of Gel:</br>
 +
100 base pair ladder</br>
 +
Ampicillin Backbone Digest 1</br>
 +
Ampicillin Backbone Digest 2</br>
 +
Kanamycin Backbone Digest</br>
 +
NAP1</br>
 +
NAP2</br>
 +
CAT1</br>
 +
CAT2</br>
 +
Blue</br>
 +
SOS</br>
 +
</br>
 +
</br>
 +
LIGATION</br>
 +
Names: Alex S, Cat O, Connie C, Reginald L, Janice C</br>
 +
Date: May 25th, 2014</br>
 +
Samples from restriction digest</br>
 +
</br>
 +
</br>
 +
MAKING AMPICILLIN/KANAMYCIN PLATES</br>
 +
Name: Christina S</br>
 +
Date: May 26th, 2014</br>
 +
</br>
 +
</br>
 +
TRANSFORMATION</br>
 +
Names: Cat O, Christina S</br>
 +
Date: May27th, 2014</br>
 +
Samples from Ligation</br>
 +
</br>
 +
</br>
 +
GROWING UP CELLS</br>
 +
Names: Christina S</br>
 +
Date: May 30th, 2014 </br>
 +
Species: E. Coli</br>
 +
Results: TBA</br>
 +
</br>
 +
</br>
 +
SETTING VARIABLE ENVIRONMENTS FOR CELLS</br>
 +
Names: Christina S, Cat O</br>
 +
Date: June 1st, 2014 </br>
 +
Species: E. Coli</br>
 +
Results: TBA</br>
 +
</br>
 +
</br>
 +
EXPERIMENTATION</br>
 +
Date: June 2nd to 5th, 2014 </br>
 +
Species: E. Coli</br>
 +
Results: TBA</br>
 +
</br>
 +
<p> Experiment Result to Be Shown At Jamboree!</p>
 +
</br>
 +
</br>
 +
<a href="http://i.imgur.com/CKv0b3k.png">See our poster! (click link)</a>
</div>
</div>
               </p>
               </p>

Latest revision as of 21:31, 6 July 2014

Lab Notebook

Starting in the 2013 Winter term, our team learned laboratory techniques and protocols. We began research on the effects of fracking and the chemical characteristics of toxic water contaminants: Catechol, naphthalene, and lead. Over the spring semester of 2014, six full time team members were working tirelessly on the project. Here, we present a summary of our daily progress and experiments.

GROWING CELL CULTURES
Names: Alex S, Reginald L, Cat O, Melody N, Janice C, Connie C, Christina S
Date: April 19th, 2014
Species: E.Coli
Part Number: BBa_J04500, BBa_J04650
Results: TBA


LIGATION
Names: Alex S, Reginald L, Cat O, Melody N, Janice C, Connie C, Christina S
Date: April 20th, 2014
Species: E.Coli
Part Number and plasmid: BBa_J04500, BBa_J04650, pSB1C3
Results: TBA


TRANSFORMATION
Names: Alex S, Reginald L, Cat O, Melody N, Janice C, Connie C, Christina S
Date: April 24th, 2014
Antibiotic: Amp and Kan
Part number and plasmid: BBa_J04500, BBa_J04650, pSB1C3
Results: TBA




GROWING CELL CULTURES
Names: Christina S, Janice C, Melody N
Date: April 29th, 2014
Species: E. Coli
Part number: BBa_K118021, BBa_J61051, BBa_K518010, BBa_K592009
Results: TBA


PICKING CELL CULTURES
Names: Alex S, Reginald L, Cat O, Melody N, Janice C, Connie C, Christina S
Date: April 30th, 2014
Species: E. Coli
Part number and plasmid: BBa_K118021, BBa_J61051, BBa_K518010, BBa_K592009, pSB1C3
Results: TBA


RESTRICTION DIGEST
Names: Alex S, Reginald L, Cat O, Melody N, Janice C, Connie C, Christina S
Date: May 18th, 2014
Species: E. Coli
Restricted: BBa_J61051, BBa_K118021
Results: TBA
Incubate 20 hours at 37°C


GROWING CELL CULTURE
Names: Christina S
Date: May 20th, 2014
Species: E. Coli


PREPARATION OF ANTIBIOTIC SOLUTION
Names: Christina S
Date: May 20th, 2014
Antibiotic: Chloramphenicol


MINIPREP
Names: Alex S, Janice C, Melody N
Date: May 21st, 2014
Mini prep: BBa_K518010_pSB1C3, BBa_J61051_pSB1C3
Species: E. Coli
Results: Worked Well


LIGATION
Names: Charis E, Reginald L, Christina S, Cat O
Date: May 21st, 2014
Species: E. Coli
Results: TBA
Tube 1- BBa_J61051_BBa_K118021_pSB1C3


MINIPREP:
Names: Christina S.
Date: May 22nd, 2014
Cultures for Miniprep: BBa_K518010_pSB1C3 (SOS), BBa_J61051_pSB1C3 (NAP), BBa_K118021 _pSB1C3 (CAT), BBa_K592009_pSB1C3 (BLUE) [samples prepped in duplicate]
Results: TBA


MEASURING DNA CONCENTRATION FROM MINIPREP
Names: Alex S, Christina S
Date: May 23rd, 2014
Samples from Miniprep
Results: DNA Concentrations
NAP1: 26.25 ng/ul
NAP2: 33.50 ng/ul
CAT1: 39.50 ng/ul
CAT2: 21.00 ng/ul
SOS1: 29.75 ng/ul
SOS2 (from mini prep on 5.21.14): 30.50 ng/ul
BLUE1: 70.25 ng/ul
BLUE2: 20.50 ng/ul


RESTRICITION DIGEST
Names: Christina S, Reginald L., Alex S.
Date: May 23rd, 2014
Samples from Miniprep


GEL ELECTROPHORESIS ON DIGEST PRODUCT
Names: Christina S
Date: May 25th, 2014
Samples from restriction digest




Loading Order of Gel:
100 base pair ladder
Ampicillin Backbone Digest 1
Ampicillin Backbone Digest 2
Kanamycin Backbone Digest
NAP1
NAP2
CAT1
CAT2
Blue
SOS


LIGATION
Names: Alex S, Cat O, Connie C, Reginald L, Janice C
Date: May 25th, 2014
Samples from restriction digest


MAKING AMPICILLIN/KANAMYCIN PLATES
Name: Christina S
Date: May 26th, 2014


TRANSFORMATION
Names: Cat O, Christina S
Date: May27th, 2014
Samples from Ligation


GROWING UP CELLS
Names: Christina S
Date: May 30th, 2014
Species: E. Coli
Results: TBA


SETTING VARIABLE ENVIRONMENTS FOR CELLS
Names: Christina S, Cat O
Date: June 1st, 2014
Species: E. Coli
Results: TBA


EXPERIMENTATION
Date: June 2nd to 5th, 2014
Species: E. Coli
Results: TBA

Experiment Result to Be Shown At Jamboree!



See our poster! (click link)

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