Team:AUC TURKEY/Notebook/Timeline

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Latest revision as of 17:34, 26 April 2015

September 16

It wasn’t easy for us to get all team members in one place who has adopted the idea of we will start to work for next year straight away right after the ‘Jamoreee. September sixteen the date schools get opened, was the meeting day for team members who were at their hometown for the summer holiday. We delayed having new members alongside the idea of working with the experience of old members still itching for iGEM.

Actually our aim was to get started quickly thanks to the tons of new ideas for our next project from everyone in the team. But that was not the case since it is against the laws of nature! The alternative ideas left from last year was always a possible b plan for us, but still we were aware of the fact that if we want to get some awards and get a high rate, these were not suitable at all. We started to think for new ideas...

September 23

We wanted make the best of the time we had ideas from previous year alongside of new and fresh ideas. We decided to work separatly since we thought it was too early to make our meetings weekly. Some members prefered scaning new datas seeking for inspiration, unlike others who chosed to improved their fantastic ideas. Our ideas were not much but modest. These are some highlights from these ideas:

-Paper cleaning bacteria: This idea was the dream of our team since the beginning. The bacteria we produced was supposed to clean the paper by simply destroying the ink on it. Incorrect outputs must hurt our team.

- Bran preventing bacteria: It is obvious that the bran produced by the outer layer of the headskin is one of the biggest issues makes mankind suffer. Variety of products in the market and dozens of clinic researches seeking for a solution shows the importance of this issue. We decided to prevent this brans made of deadskin with the bacterium we produced. We gave up this idea because brans which are the main food resource of the mites could go through some metabolic processes.

- Bacterium to support Plants defense: This project started with the idea of protecting plants from invasions of insects and then aimed to program our bacterium in a way that they will make sure plant starts to defend itself earlier and stronger.

- E.coli designed for X-treme conditions: E.coli the worker of the synthetic biology they ara likely to get effected by x-treme conditions and give unwanted bad results for the project. Increasing the durability of E.coli would encourage its usage in the world ofsynthetic biology. We aimed to create an alternative system which was going to increse its durability with the part that we produce alongside of the gens of the project. We are convienced on the fact that using the genom of a bacteria which was in the records book of guiness and resistant to x-treme conditions such as “deinococcus radiodurans”.

November 16

-We did brainstorming

January 22

-Everyone came with 5 project ideas at least

March 3

-We started to net our project

April 27

-We decide our Project ‘’DegradEcolor’’

1 Haziran

-We sterilized our lab and materials

4 Haziran

-We bought our airline ticket.

7 Haziran

-We went to sponsorship interview.We agred with BTC construction company

10 Haziran

We made liquid culture

11 Haziran

-We prepare competent cells

12 Haziran

We did transformation HRP and LIP parts

13 Haziran

-We made liquid culture from last day’s transformation’s colonies and learnt how can we do western blot protocol.

14 Haziran

-We did isolation of BL21 strain

15 Haziran

CBB results

16 Haziran

-Western Blot

17 Haziran

-Wiki preperation

18 Haziran

-We did functional assay

19 Haziran

We did wiki and human practises

20 Haziran

WIKIFREEEEZE

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-<body>
-<h1>Notebook</h1>
+<body lang=TR>
-<div>Use arrow keys to turn notebook's pages.</div><br/><br/>
-<div id="mybook">
+<div class=WordSection1>
-	        <div>
-<center>
+<p style='margin:0cm;margin-bottom:.0001pt'><span style='font-family:"Calibri","sans-serif"'>September
-	            <h1>AUC TURKEY<br/>iGEM 2013 HS<br/>NOTEBOOK</h1>
+</span></p>
-<img src="https://static.igem.org/mediawiki/2013hs/7/7c/AUC_TURKEY_LOGO.png" style="width:200px;"/>
-</center>
+<p style='margin:0cm;margin-bottom:.0001pt'><span style='font-family:"Calibri","sans-serif"'>It
-	        </div>
+wasn’t easy for us to get all team members in one place who has adopted the
-	        <div>
+idea of we will start to work for next year straight away right after the
-	        </div>
+‘Jamoreee. September sixteen the date schools get opened, was the meeting day
-<div><h2>March</h2></div>
+for team members who were at their hometown for the summer holiday. We delayed
-<div>
+having new members alongside the idea of working with the experience of old
-</div>
+members still itching for iGEM.</span></p>
-<div>
-	            <h3>1 March</h3>
+<p style='margin:0cm;margin-bottom:.0001pt'><span style='font-family:"Calibri","sans-serif"'>Actually
-<ul><li>Today marks the official start of our 2013 iGEM adventure. We hope that today becomes a start for the many great things to come.</li><li>The reason why today was the "official" start is because our first trip to Turgut Ozal University for this year was today. We met with Mustafa Semih Elitok, our advisor, to discuss the current situation and our project ideas.</li><li>The 2 new members of the team got acquinted with our advisor.
+our aim was to get started quickly thanks to the tons of new ideas for our next
-</li><li>Out of today's discussions, we have decided to work on the following project ideas:
+project from everyone in the team. But that was not the case since it is
-<ul>
+against the laws of nature! The alternative ideas left from last year was
-<li><b>Cyanide Disposing Bacteria:</b> Cemal and Abdulkadir will work on this idea.</li>
+always a possible b plan for us, but still we were aware of the fact that if we
-<li><b>Miner Bacteria:</b> Alperen and Furkan Sacit will work on this idea.</li>
+want to get some awards and get a high rate, these were not suitable at all. We
-<li><b>Thermobacter:</b> Fatih will work on this idea.</li>
+started to think for new ideas...</span></p>
-<li><b>Musilage Bacter:</b> İbrahim and Lashynbek will work on this idea.</li>
-</ul>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-</li>
+normal'><span style='font-size:12.0pt'>September 23</span></p>
-</ul>
-</div>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<div><h3>8 March</h3>
+normal'><span style='font-size:12.0pt'>We wanted make the best of the time we
-<ul><li>We continued discussing our project ideas.</li>
+had ideas from previous year alongside of new and fresh ideas. We decided to
-<li>As a result of the improvising, we decided to conduct research on only these 3 ideas for now:
+work separatly since we thought it was too early to make our meetings weekly.
-<ul><li><b>Electrocortecoli</b><br/>
+Some members prefered scaning new datas seeking for inspiration, unlike others
-The E.coli that we plan to produce will be negatively charged. Combined with an adesion molecule, the E.coli will bind to a specific cell thus creating an overall negatively charged system. This system can then be easily destroyed in the presence of a charged unit. Through this, sepcific cells can be targeted with E.coli and then be easily destroyed.
+who chosed to improved their fantastic ideas. Our ideas were not much but
-The list of the research required for us to do is as follows:
+modest. These are some highlights from these ideas:</span></p>
-<ul><li>Finding the sequence of an adesion molecule which can be implantabled to E.coli</li>
-<li>Finding a way to electrocharge our E.coli</li>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<li>Searching iGEM to see if something which could be of use was done before</li>
+normal'><span style='font-size:12.0pt'>&nbsp;</span></p>
-</ul>
-</ul>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-</ul></div>
+normal'><span style='font-size:12.0pt'>-Paper cleaning bacteria: This idea was
-<div>
+the dream of our team since the beginning. The bacteria we produced was
-<ul>
+supposed to clean the paper by simply destroying the ink on it. Incorrect
-<li><b>Thermobacter</b><br/>
+outputs must hurt our team.</span></p>
-The thermobacter will be able to sense temperature and react to it through producing urease which will cause the urea we add into the environment to be converted to uric acid. This reaction is endothermic and will result in the environment cooling down. Through this system, an automatic cooler through bacteria will be formed.
-The list of the research required for us to do is as follows:
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<ul><li>Searching to see if this project was don before or if there is anything in iGEM that could be of use</li>
+normal'><span style='font-size:12.0pt'>&nbsp;</span></p>
-<li>Finding the sequence for urease enzyme</li>
-<li>Designating several ententities of the RNA Thermometer which can be of use</li>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-</ul>
+normal'><span style='font-size:12.0pt'>- Bran preventing bacteria: It is
-<li><b>Mineral-Fuser Bacter</b></li></ul></div>
+obvious that the bran produced by the outer layer of the headskin is one of the
-<div><h3>14 March</h3>
+biggest issues makes mankind suffer. Variety of products in the market and
-Today we discussed:
+dozens of clinic researches seeking for a solution shows the importance of this
-<ul><li>Project Ideas</li>
+issue. We decided to prevent this brans made of deadskin with the bacterium we
-<li>Sponsorship</li>
+produced. We gave up this idea because brans which are the main food resource
-<li>Our Previous iGEM Experience</li></ul>
+of the mites could go through some metabolic processes.</span></p>
-<b>Project Ideas</b><br/>
-<ul><li><b>Electrocortecoli:</b> We were not able to discuss much about it as we hadn't been successful in finding information. We know the existence of an adhesion molecule for mammal cells in iGEM called cadherin but it is for mammal cells. Also, we still have no clue on how to electrocharge our E.coli.</li>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<li><b>Thermobacter:</b> This project looks like it is in the bag. The parts are already in iGEM (RNA Thermometer and urease enzyme). We won't have to worry much about the research for the RNA Thermometer.</li>
+normal'><span style='font-size:12.0pt'>&nbsp;</span></p>
-</ul></div>
-<div><ul><li><b>Mineral-Fuser Bacter:</b> The research we conducted over the last week showed that it will be extremely difficult to do this as a project as there is already a lack of information on this matter. The only thing that we could find was that a certain type of bacteria has this property but scientists haven't identified how they do it.</li>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<li><b>A Revival of our old Idea Stone Miner:</b> In a multiple choice question of an exam, Furkan Beştepe read about a type of coral called "Lithophaga". This coral was found in Naples and had been designated as the cause of the degrading of historical buildings. This coral can eat through stone!</li></ul></div>
+normal'><span style='font-size:12.0pt'>- Bacterium to support Plants defense:
-<div>
+This project started with the idea of protecting plants from invasions of
-<h3>15 March</h3>
+insects and then aimed to program our bacterium in a way that they will make
-<ul>
+sure plant starts to defend itself earlier and stronger.</span></p>
-<li>The discussions we had in school yesterday were pretty much carried out but this time with our advisor.</li>
-<li>Also, we did our new tradition once more. What is this you may ask. Well it is 2 minutes of random idea burst without reasoning. Here are the results: <i>Neurobacter, Painkiller, Writer, Clock-o-Bacter, Methanecoli, Rust Eater, Decayer, Tooth Paste, Tooth Filling, Balloon Inflator, CO Filter, Imnosia Bacter, SleepingBactuety, Photosynthetic, Fire Figther, Quartz Watch, Tire Repair, Anti-Bacterial-Bacter, UV to Visible Light Converter, Barocoli, Cooler, Optic Glass, Virus Detector, Infrared Bacter, Jammer, Psychic Bacter, Crystalecoli, Balance, Sewing Bacter, MRBacter, Pixellence, Glass Eater, Asphalt 4, Vacuum, Sun Lotion, Painter, Hemoglobin Detector, PCR, Anti-Radioctivity Bacter, Bactomembrane, Glue Bacter, Plastic Eater, Paper Consumer</i></li>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-</ul>
+normal'><span style='font-size:12.0pt'>&nbsp;</span></p>
-</div>
-<div><h3>22 March</h3>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<ul><li>As of today, considering the time and difficulty, we decided to focus only on Thermobacter. To improve thermobacter, we realized that we had add at least one part to iGEM. As a new part we could do:
+normal'><span style='font-size:12.0pt'>- E.coli designed for X-treme
-<ol><li>A New Type of RNA Thermometer</li>
+conditions: E.coli the worker of the synthetic biology they ara likely to get
-<li>Another addition to the project</li>
+effected by x-treme conditions and give unwanted bad results for the project.
-</ol></li>
+Increasing the durability of E.coli would encourage its usage in the world
-<li>With the remaining time, we came up with human practice ideas:
+ofsynthetic biology. We aimed to create an alternative system which was going
-<ul><li>Visiting schools</li>
+to increse its durability with the part that we produce alongside of the gens
-<li>Visiting businessmen</li>
+of the project. We are convienced on the fact that using the genom of a
-<li>Visiting corporations</li>
+bacteria which was in the records book of guiness and resistant to x-treme
-<li>Conducting surveys</li>
+conditions such as “<i>deinococcus radiodurans”. </i></span></p>
-<li>Preparing handouts</li>
-<li>Doing advertisements</li>
+<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'> </span><span
-<li>iGEMan</li>
+style='font-size:12.0pt;line-height:115%'>November 16</span></p>
-<li>Making a board game</li>
-<li>Making a computer game</li>
+<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>-We did
-<li>Preparing Canvas Times 2</li>
+brainstorming</span></p>
-<li>Doing Videos</li>
-</ul>
+<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>January 22</span></p>
-</div>
-<div><h3>25 March</h3>
+<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>-Everyone
-Today, we finally constructed a system. In this system, the RNA thermometer will be activated forcing the production of the urease enzyme serving the purpose of “bactocooling”.<br/>
+came with 5 project ideas at least</span></p>
-This is the clearified system.<br/>
-We also designed the parts that we will be adding to the registry.<br/>
+<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>March 3</span></p>
-<h3>29 March</h3>
-Something so unpleasant became apparent to us today. Mustafa realized that the Brown Stanford 2008 Team had not submitted the sequence of the urease BioBrick to the parts registry. So basically, we cannot use the part in comfort as we now are not sure if the urease part will work or not.
+<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>-We started
-So we had to prepare a new work plan to desigante what we should do from now on.
+to net our project</span></p>
-<ol><li>Today we submitted the <b>Project Description</b> which was due for Sunday.</li>
-<li>We need to clarify the part design for our project.</li>
+<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>April 27</span></p>
-</ol>
-</div>
+<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>-We decide
-<div>
+our Project ‘’DegradEcolor’’</span></p>
-<ol start="3">
-<li>We need to somehow obtain the base sequence for urease or we will be forced to thrust the iGEM Kit.</li>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<li>We must complete the human practices before the heavy workload begins.</li>
+normal'><span style='font-size:12.0pt;color:black'>1 Haziran</span></p>
-</ol>
-For the construction <br/>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-So now we have to decide what we will do with the urease part.<br/>
+normal'><span style='font-size:12.0pt;color:black'>-We sterilized our lab and
-There is also the project description.<br/>
+materials</span></p>
-<b>Project Description:</b> “In Jamboree 2013, we hope to present you the bactocooler that we have started to design. We shall add a part to our system which shall detect “thermal stimuli”. This may be a system that will be activated at a certain temperature.
-As a result of our thermal stimuli, we want our cooler system to be activated. To decrease the temperature, we are thinking of breaking down an organic compound with an endothermic reaction.
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-Overall, we can state that we intend to make a synthetic cooler which will be activated after a specific temperature”.
+normal'><span style='font-size:12.0pt;color:black'>4 Haziran</span></p>
-</div>
-<div>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-</div>
+normal'><span style='font-size:12.0pt;color:black'>-We bought our airline
-<div>
+ticket.</span></p>
-<h2>April</h2>
-</div>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<div></div>
+normal'><span style='font-size:12.0pt;color:black'>7 Haziran </span></p>
-<div>
-<h3>1 April</h3>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-Today, we focused on mainly human practices.
+normal'><span style='font-size:12.0pt;color:black'>-We went to sponsorship
-As a result of a meeting, we have specified the human practices we will be doing.
+interview.We agred with BTC construction company</span></p>
-<ul><li><b>Board Game/ PC Game:</b> Furkan Sacit, Fatih, Alperen, Alihan</li>
-<li><b>Advertisements:</b> Abdulkadir</li>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<li><b>iGEMan:</b> Fatih</li>
+normal'><span style='font-size:12.0pt;color:black'>10 Haziran </span></p>
-<li><b>iGEM cookies:</b> Cemal</li>
-<li><b>Spreading iGEM around:</b> İbrahim, Abdulkadir</li>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<li><b>TRT Documentary:</b> Fatih Sacit</li>
+normal'><span style='font-size:12.0pt;color:black'>We made liquid culture</span></p>
-<li><b>Day with the firefighters:</b> Alperen</li>
-<li><b>Parody Videos:</b> Fatih</li>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<li><b>iGEMism:</b> Alperen, Cemal
+normal'><span style='font-size:12.0pt;color:black'>11 Haziran</span></p>
-<ol type="a"><li>Socialogic articles</li>
-<li>Propaganda posters</li>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<li>Videos</li>
+normal'><span style='font-size:12.0pt;color:black'>-We prepare competent cells</span></p>
-</ol></li>
-</ul>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-</div>
+normal'><span style='font-size:12.0pt;color:black'>12 Haziran</span></p>
-<div>
-<h3>8 April</h3>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-In the university, we designated the deadlines for the human practices with our instructor.
+normal'><span style='font-size:12.0pt;color:black'>We did transformation HRP
-<ul>
+and LIP parts</span></p>
-<li><b>Documentary (Furkan Sacit)</b>; If he can get approved from TRT, if he cannot, we won’t.</li>
-<li><b>Introduction of iGEM (Abdulkadir, İbrahim)</b>; The deadline is 29 April 2013 <b>Schools:</b> TED, Bilkent, Arı, MEV</li>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<li><b>A day with the firefighters (Alperen, Fatih)</b>; The deadline is 29 April 2013</li>
+normal'><span style='font-size:12.0pt;color:black'>13 Haziran</span></p>
-<li><b>iGEMism</b>s
-<ul><li><b>iGEMan (General Mascot)</b></li>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<li><b>Cookies (Our Mothers)</b></li>
+normal'><span style='font-size:12.0pt;color:black'>-We made liquid culture from
-<li><b>Poster (Fatih)</b>; deadline is 29 April</li>
+last day’s transformation’s colonies and learnt how can we do western blot
-<li><b>Videos (Fatih)</b></li>
+protocol.</span></p>
-<li><b>PC Game (Alihan)</b>; deadline is 15 May</li>
-</ul>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<li><b>Collaboration</b></li>
+normal'><span style='font-size:12.0pt;color:black'>14 Haziran</span></p>
-</ul>
-</div>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<div>
+normal'><span style='font-size:12.0pt;color:black'>-We did isolation of BL21
-<h3>12 April</h3>
+strain</span></p>
-We finally got to put together the urease missing parts.<br/>
-We used computer program to configure the binding sites. There were no exceptions in the base sequence.<br/>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<b>Also, we discussed some of the poster ideas for iGEMism:</b>
+normal'><span style='font-size:12.0pt;color:black'>15 Haziran</span></p>
-<ul><li>A poster with two bacteria shaking hands.</li>
-<li>Uncle Sam like <b>iGEM Needs You</b> poster.</li>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<li><b>We must interact</b> poster.</li>
+normal'><span style='font-size:12.0pt;color:black'>CBB results</span></p>
-<li>A poster that a bacteria grabs a falling man.</li>
-</ul>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<b>Another idea was thought out by Fatih:</b><br/>
+normal'><span style='font-size:12.0pt;color:black'>16 Haziran</span></p>
-A page in the wiki in which you add properties to your bacteria and visually experience it.
-</div>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<div>
+normal'><span style='font-size:12.0pt;color:black'>-Western Blot </span></p>
-<h3>16 April</h3>
-We were planning to prepare on this school-free day but sadly, the required equipment were still not available.
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-So as a result, we prepared things for iGEMism and gave people their duties:
+normal'><span style='font-size:12.0pt;color:black'>17 Haziran</span></p>
-<ul><li><b>Abstract:</b> İbrahim</li>
-<li><b>Data Page:</b> Lashynbek</li>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<li><b>Human Practice:</b> Abdulkadir</li>
+normal'><span style='font-size:12.0pt;color:black'>-Wiki preperation</span></p>
-<li><b>RNA Thermometer:</b> Fatih</li>
-<li><b>Urease:</b> Alperen</li>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<li><b>Parts:</b> Furkan Sacit</li>
+normal'><span style='font-size:12.0pt;color:black'>18 Haziran</span></p>
-<li><b>Overview:</b> Cemal</li>
-<li><b>Notebook:</b> Fatih</li>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-</ul>
+normal'><span style='font-size:12.0pt;color:black'>-We did functional assay</span></p>
-</div>
-<div>
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<h3>19 April</h3>
+normal'><span style='font-size:12.0pt;color:black'>19 Haziran</span></p>
-We were planning to prepare competent cells today but we learned that we had no liquid nitrogen to prepare it.<br/>
-We hope to order it soon and finally start doing our lab experiments.
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<h3>24 April</h3>
+normal'><span style='font-size:12.0pt;color:black'>We did wiki and human
-We still did not have liquified Nitrogen, so we instead used other competent cells to do transformation.<br/>
+practises</span></p>
-We did our first experiment of the year.<br/>
-Also, we had a “kermes” in the morning to fundraise for our project.
+<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-<h3>25 April</h3>
+normal'><span style='font-size:12.0pt;color:black'>20 Haziran </span></p>
-Today was the second day of our “kermes”. Through the purehases and the big help from our teachers, we acquired <b>700 TL</b>. We want to thank everyone for their wonderful support.<br/>
-Sadly, yesterdays transformation was not successful.
+<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%;color:black'>WIKIFREEEEZE</span></p>
-</div>
-<div>
-<h3>30 April</h3>
-Today, we did the transformation of 2 RFP plates.
-Both of the plates had yield but 1 had only 3 colonies and the other one had only 1.
-</div>
-<div></div>
-<div></div>
-<div>
-<h2>May</h2>
-</div>
-<div></div>
-<div>
-<h3>4 May</h3>
-Today, we prepared liquid culture. Hope it is successful.
-<h3>5 May</h3>
-We did experiments from the morning the evening. Almost all of them were successful with the exception of ligestion. We realized that the plasmid were not cut during electrophoresis. At least the nanodrop results showed that  it was ok.
-<h3>11 May</h3>
-Today was a long day. From 1.p.m to 10.30 p.m, we prepared compatent cells. At least it was worth the effort, the compatent cells have great efficiency.
-<h3>12 May</h3>
-E-BIKO
-<h3>18 May</h3>
-Just as a safety measure, we wanted to learn if whether our compatents were as effcient as we thought isolation.
-</div>
-<div>
-<h3>21 May</h3>
-We had a meeting in the school. Everybody was assigned their jobs. Also we prepared certain things for the wiki such as the graphics and writings.
-</div>
-<div></div>
-<div></div>
-<div>
-<h2>June</h2>
-</div><div></div>
-<div>
-<h3>1 June</h3>
-We searched for plane tickets and found several. 2 of the team members bought their tickets.
-Also, we prepared a draft for the RNA Thermometer text we will put into our wiki.
-<h3>3 June</h3>
-A big part of the rest of the group got their plane tickets today.
-Also RNA Thermometer was edited.
-<h3>4 June</h3>
-Alihan started to make the flash game. He writed some of the code.
-Also, we prepared the story of our comics today.
-<h3>5 June</h3>
-Photos for the comics were taken.
-Also, Alihan continued to do the flash game.
-Plus, 2 iGEMism posters were prepared today.
-Also on the side, Urease draft for the wiki was prepared.
-</div><div>
-<h3>6 June</h3>
-We continued to prepare for the comics and flash game.
-RNA Thermometer and Urease were edited.
-We also sketched out our experiment process for the next 2 weeks.
-<h3>7 June</h3>
-We continued to prepare the flash game and also discussed the experiments we should do.
-Our genes arrived from the company we ordered and we immidiately started transformating them and let them go for overnight incubation.
-<h3>8 June</h3>
-Liquid Culture for RFP was prepared.
-RNA Thermometer text was edited and the flash game was continued.
-We did Photoshop and also iGEMism posters today.
-<h3>9 June</h3>
-RFP-IbpB-ROSE Isolation
-RFP-IbpB-ROSE Digestion
-RFP-IbpB-ROSE Electrophoresis
-Also we continued to do the comics and did more Photoshopping.
-</div><div>
-<h3>10 June</h3>
-We did spectrophotometer calculations with 0,6 OD. There was RFP produced as a result and we dropped the OD to 0.3.
-<h3>11 June</h3>
-Spectrophotometer again with 0.3 OD
-<h3>12 June</h3>
-Spectrophotometer again with 0.3 OD
-<h3>13 June</h3>
-Spectrophotometer again with 0.3 OD
-IbpB Isolation, Digestion and Electrophoresis
-RFP-1C Liquid Culture Preparation
-<h3>14 June</h3>
-Spectrophotometer again with 0.3 OD
-RFP-1C Isolation, Digestion and Electrophoresis
-Liquid Culture of IbpB and ROSE
-</div><div>
-<h3>15 June</h3>
-IbpB – ROSE Isolation, Digestion and Electrophoresis
-<h3>17 June</h3>
-We have taken  IbpB  1, 2, 3 and RFP 1, RFP 2 and RFP 3  liquid cultures and we have done isolation, digestion and electrophoresis. According to the electrophosesis result our genes base length was right but we couldn’t understand why our IbpB liquid culture didn’t show red colour.
-<h3>18 June</h3>
-At the end of  36 hours  incubation,we transferred  our Ibpb and RFP liquid culture to 2 ml eppendorves and  we centrifuged them.And IbpBs and RFPs which in 42 centigrade incubating.IbpB and RFPs which have been incubating  in room temprature RFPs pellets were red.But IbpB’s pellets were white. Because of this we proved our experiment and IbpB thermometer activated in 42 centigrade but IbpB thermometer didn’t activate in room temperature.
-</div><div>
-The plasmid named as 500 has been digested and electrophorised. However, fortunately for us base length was correct.<br/><br/>
-We did ligestion of IbpB and 500 parts. We cultivated in plate which  antibiotic is chloramfenicol.<br/><br/>
-We took ROSE’s liquid culture and their pellets were white. But it theoretically should be red. We did isolation,digestion and electrophoresis.
-<h3>19 June</h3>
-The transformation which we had done in yesterday was failed.We repeated the transformation again. We calculated the OD of the RFP and IbpB’s at 27 C and 37 C. According to their OD values, we diluted them. We then transferred them to the 96 well plate.<br/><br/>
-We were going to measure them with the plate reader but it’s filter was broken. We couldn’t do it.<br/><br/>
-Also, we looked at the specimens we took from the IbpB and RFP under the fluorescent microscope.
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-<h3>20 June</h3>
-Our transformation didn’t work out again. We left them for over-night incubation once more.<br/><br/>
-We tried to fix our bacteria in the fluorescent microscope once more.
-<h3>21 June</h3>
-Microscope again<br/><br/>
-Ligation and Transformation Again
-<h3>21-22 June</h3>
-Wiki Freeze!
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