Team:NGSS TR/catechol.html

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<blockquote>
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  <h1>PROTOCOLS</h1>
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  <blockquote>
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    <h2><span class="gfdg">2.CATECHOL PROTOCOL</span></h2>
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    <p><br />
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      Catechol assay is  performed in the plate reader on a 96 well plate<br />
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      Each well must be filled  with 100um of solution<br />
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      Usually use 90ul of cell  culture and 10um of catechol solution<br />
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      Catechol stock solution is  at 100mM concentration. And when added to the well we have a 10fold dilution.  For example if an aliquot concentration of 1mM catechol is made, which would be  used for assay, when the well is added catechol drops to a concentration of  0.1mM.<br />
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      Always dilute catechol  with H2O.<br />
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      Always have a blank of  90ul medium (the one which you grew the cells overnight) with 10ul catechol  solution<br />
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      Always have a negative of  90ul growing cells and 10ul of H2O.</p>
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    <p>Reference: https://2010.igem.org/Team:Imperial_College_London</p>
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Latest revision as of 11:20, 20 June 2014

PROTOCOLS

2.CATECHOL PROTOCOL


Catechol assay is performed in the plate reader on a 96 well plate
Each well must be filled with 100um of solution
Usually use 90ul of cell culture and 10um of catechol solution
Catechol stock solution is at 100mM concentration. And when added to the well we have a 10fold dilution. For example if an aliquot concentration of 1mM catechol is made, which would be used for assay, when the well is added catechol drops to a concentration of 0.1mM.
Always dilute catechol with H2O.
Always have a blank of 90ul medium (the one which you grew the cells overnight) with 10ul catechol solution
Always have a negative of 90ul growing cells and 10ul of H2O.

Reference: https://2010.igem.org/Team:Imperial_College_London

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