Team:Shenzhen SZMS

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|You can write a background of your team here.  Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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|align=center width=12.5%|[[Team:Shenzhen SZMS|Home]]
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|[[Image:Shenzhen_SZMS_logo.png|200px|right|frame]]
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|align=center width=12.5%|[[Team:Shenzhen SZMS/Team Members|Team Members]]
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|align=center width=12.5%|[[Team:Shenzhen SZMS/Project|Project]]
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|align=center width=12.5%|[[Team:Shenzhen SZMS/Modelling|Modelling]]
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''Tell us more about your project. Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|align=center width=12.5%|[[Team:Shenzhen SZMS/Lab|Lab]]
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|[[Image:Shenzhen_SZMS_team.png|right|frame|Your team picture]]
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|align=center width=12.5%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]
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|align=center width=12.5%|[[Team:Shenzhen SZMS/Gallery|Gallery]]
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|align=center width=12.5%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]
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|align="center"|[[Team:Shenzhen_SZMS | Team Shenzhen_SZMS]]
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<!--- Team Information Link --->
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[https://2014hs.igem.org/Main_Page <<Back to wiki main page]
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!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=Shenzhen_SZMS Official Team Profile]
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[[File:Shenzhongigem2014.png|400px|right]]
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Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''.
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===Team===
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==Team==
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''For more information, check the following websites:
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Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.
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*[http://www.shenzhong.net/ school offical website (in Chinese)]
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*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]
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*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School Wikipedia]''
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===Project===
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===Attributions===
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Our project is to create an '''E. coli Plant-sitter''' toolkit whose functions include temperature moderation, fungi inhibition, and hormone provision. We aim to achieve smart manipulation of plant growth introduced through synthetic biology. We hope that our toolkit will simplify the process of plant cultivation and make the technology of synthetic biology accessible to people’s daily life.  
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Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].
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====Temperature Moderation====
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==Project==
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It is observed that a considerable number of domestic plant-deaths are caused by negligence of temperature control in overly hot or cold environments. Therefore, we came up with a system which reduces these death-rates and aids the healthy growth of plants by keeping the temperature within a suitable range.
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Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.
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Our mechanism of temperature moderation, including a warming system and a cooling system, aims to keep the temperature between 15℃ and 30℃ (the fittest temperature range for most plants) by using RNA thermometers and two controllable chemical reactions, one exothermic and the other endothermic. During the cooling process, the RNA thermometer is activated when the surrounding temperature reaches 30℃, which encodes phytoene dehydrogenase (PDS). The encoded enzyme then dehydrates phytoene to form phytofluene, initiating an endothermic reaction which absorbs energy and cools down the environment. When the temperature drops to around 20℃, a second RNA thermometer is then activated, followed by an inhibitor which ends the translation of PDS and thus the absorption of heat. During the warming process, the RNA thermometer is activated when the surrounding temperature reaches 15℃, which encodes elastase. The encoded enzyme then hydrolyzes protein, initiating an exothermic reaction which releases energy and warms up the environment. When the temperature rises up to around 20℃, a second RNA thermometer is activated, followed by an inhibitor which ends the translation of elastase and thus the release of heat.
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Through this system of temperature control, we are able to provide an agreeable temperature range which ensures the plants of their healthy development.
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====Fungi Inhibition====
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Read more:[[Team:Shenzhen SZMS/Project]]
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Since our plants and e.coli are exposed to non-sterile condition as in everyday life, they are subjects to contamination by fungi in the air. In order to guarantee the healthy growth of both plants and e.coli, we designed a system which autonomously defends them against fungi contamination by attacking fungi’s cell walls, which primarily consist of chitin. Our aim is to degrade the chitin in the fungi by synthesizing chitinase. With the chitinase coding sequence being inserted in the plasmid, the e.coli will regularly produce chitinase, which attacks the fungi’s cell walls, hinders the normal chitin synthesis, disturbs the life activities of fungi, eliminates fungi contamination, and thus ensures a safe environment for both our plants and our e.coli
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[[File:kanshu.png|500px]]
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====Hormone Provision====
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==Lab==
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In order to fulfill our plan of smart cultivation, certain vital plant-growth hormones should be provided to further ensure the optimum development of plants. Auxin, GA, CTK and ethyne are all important hormones in plant-growth, among which we chose auxin for its comprehensive promotion in shoots elongation, root initiation and cell division. Without external sources from which hormones can be derived, it is difficult for a single plant by itself to synthesize sufficient hormones to guarantee its optimal development. Therefore, we employ e.coli as the source of auxin by inserting auxin’s coding sequence into the plasmid. In this way, we are able to construct a convenient auxin supply which serves as a supportive aid to the plants’ development.
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Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br>
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[[File:Shenzhen_mengtu.png|600px]]
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===Notebook===
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==Modelling==
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Show us how you spent your days.
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We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]
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<br>
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[[File:jianmomengtu.png|500px]]
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==Safety==
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Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]
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<br>
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[[File:anquan.png|500px]]
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===Results/Conclusions===
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==Human Practice==
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What did you achieve over the course of your semester?
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{| align=right
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|[[File:helloworld1.jpeg|200px]]
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|[[File:helloworld2.jpeg|200px]]
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|[[File:helloworld3.jpeg|200px]]
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|[[File:helloworld4.jpeg|200px]]
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Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!
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===Safety===
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Sincere greeting from the iGem lab in Shenzhen Middle School!
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What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
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Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."
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===Attributions===
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Our human practice plan includes:
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Who worked on what?
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#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]
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#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]
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#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]
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==Fun==
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Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)
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===Human Practices===
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Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!
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What impact does/will your project have on the public? 
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==More Info==
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===Fun!===
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*[http://www.shenzhong.net/ school offical website (in Chinese)]
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What was your favorite team snack?? Have a picture of your team mascot?
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*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]
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*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]
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<forum_subtle />
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Latest revision as of 03:36, 21 June 2014

Home Team Members Project Modelling Lab Safety Gallery Human Practice

<<Back to wiki main page

IGEM basic Logo white stylized.png Shenzhen SZMS logo.png
Shenzhongigem2014.png

Welcome to homepage of Team:Shenzhen SZMS! Our project is called E. coli Plant-sitter.

Contents

Team

Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.

Attributions

Hey here! Check out our 17 members!Team:Shenzhen SZMS/Team Members.

Project

Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.

Read more:Team:Shenzhen SZMS/Project
Kanshu.png

Lab

Wanna find more about how we bulid our plasmid? Check here:Team:Shenzhen SZMS/Lab
Shenzhen mengtu.png

Modelling

We bulit a model to estimate how the enzyme works. Check here:Team:Shenzhen SZMS/Modelling
Jianmomengtu.png

Safety

Check our answers to the Safety Questions here:Team:Shenzhen SZMS/iGem Safety Q&A
Anquan.png

Human Practice

Helloworld1.jpeg
Helloworld2.jpeg
Helloworld3.jpeg
Helloworld4.jpeg

Come and meet the friend of our E.coli babysitter, E.coli the Fluorescent!

Sincere greeting from the iGem lab in Shenzhen Middle School!

Since all of our E.coli the Fluorescent are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."

Our human practice plan includes:

  1. Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014.
    Details:Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day
  2. Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis.
    Details:Team:Shenzhen SZMS/Human Practice/Laboratory Open Day
  3. Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology.
    Details:Team:Shenzhen SZMS/Human Practice/Lectures

Fun

Theme song for our project called E.coli Plant-sitter heals the world! A song that always arises in our minds whenever we think of iGem :)

Find more photos here:Team:Shenzhen SZMS/Gallery!

More Info

  • [http://www.shenzhong.net/ school offical website (in Chinese)]
  • [http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]
  • [http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]
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