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| - | <!doctype html>
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| - | <html>
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| - | <head>
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| - | <meta charset="utf-8">
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| - | <title>iGEM San Diego - Project</title>
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| - | </head>
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| - | <body>
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| - | <div id="navbar-wrapper" class="nav">
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| - | <div id="inside-wrapper">
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| - | <div id="logo-holder">
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| - | Logo Will Go Here
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| - | </div>
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| - | <nav id="navbar">
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| - | <a style="padding-left: 0;" href="index.html">Home</a>
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| - | <a href="project.html">Project</a>
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| - | <a href="themteam.html">The Team</a>
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| - | <a href="journal.html">Journal</a>
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| - | <a href="results.html">Results</a>
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| - | <a href="outreach.html" style="border-bottom: 1px solid #e8e8e8;">Outreach</a>
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| - | <a href="safety.html">Safety</a>
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| - | <a href="igem.org" style="padding-right: 0;">iGEM</a>
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| - | <div id="inside-wrapper-second">
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| - | <div id="secondnav">
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| - | <a href="#header-holder">Collaboration</a>
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| - | <a href="#linkheadertwo">Form an iGEM Team</a>
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| - | </div>
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| - | </div>
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| - | </div>
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| - |
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| - | <!--<div id="phase-wrapper">
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| - | <div id="first-phase">
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| - | <img src="images/front page diagram white 3.png" width="400px" height="400px"/><br />
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| - | <a href="#header-text">See Statistics</a>
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| - | </div>-->
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| - | <div id="header-holder">
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| - | <div id="header-text">
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| - | Collaboration<br />
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| - | <span style="font-family: 'HelveticaNeueLT Pro 25 UltLt'; font-size: 18px;">Collaboration within our Project</span>
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| - | </div>
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| - | <div id="second-phase-wrapper">
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| - | <div id="second-phase">
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| - | <div id="first-statistic">
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| - | <img src="images/600px-White_Globe_Icon.png" width="150px" height="150px" /><br />
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| - | AflatoxinB1-Detoxifizyme is produced by <a style="text-decoration:underline; color:black; font-size: 18px; background: none; padding:0px;" href="http://www.ncbi.nlm.nih.gov/pubmed/21239152">Armillariella Tabescens</a> intracellularly. Its gene is analyzed to have no introns and has been recombined into a plasmid <a style="text-decoration:underline; color:black; font-size: 18px; background: none; padding:0px;" href="https://www.legionpatent.com/patents/7695751/">before</a>. Extraction is rather difficult and commercialization has not been able to be done yet.
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| - | </div>
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| - | <div id="second-statistic">
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| - | <img src="images/600px-White_Globe_Icon.png" width="150px" height="150px" /><br />
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| - | Zearalenone Hydorlase is produced by Clonostachys Rosea. Its gene is also analyzed to have no introns and has been recombined into a plasmid <a style="text-decoration:underline; color:black; font-size: 18px; background: none; padding:0px;" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1222652/">before</a>. Similar to ADTZ, commercialization has not been done yet.
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| - | <div id="third-phase-button"><br /></div>
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| - | <div id="third-statistic">
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| - | <img src="images/600px-White_Globe_Icon.png" width="150px" height="150px" /><br />
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| - | Natural E. coli secretes various proteins by signal peptides that recognize translocons. We focus on the SEC pathway that lets the protein fold in the periplasm first, as it usually results in more correct folding, correct formation of disulfide bridges, minimizes degradation, and is less likely to result in accumulation.
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| - | <div id="header-holder">
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| - | <div id="linkheadertwo"></div>
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| - | <div id="header-text">
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| - | How to Form an iGEM team<br />
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| - | <span style="font-family: 'HelveticaNeueLT Pro 25 UltLt'; font-size: 18px;">How to form an iGEM team</span>
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| - | </div>
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| - | <div id="phase-wrapper" style="background:url(images/blue-blue.png);">
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| - | <div id="third-phase">
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| - | <img src="images/image.png" width="1070" height="292"/>
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| - | <div id="third-text"> Our idea is to attach signal peptides to the N-terminus of the detoxifying enzymes. We use alpha amylase <a href="http://www.signalpeptide.com/index.php?sess=&m=listspdb_bacteria&s=details&id=37&listname=">signal peptides</a> and
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| - | beta-lactamase <a href="http://www.signalpeptide.com/index.php?sess=&m=listspdb_bacteria&s=details&id=34&listname=">signal peptides</a>. The original proteins for these signal peptides are secreted out naturally by E. Coli, especially in strain K that we use for our project. They both follow sec-dependent pathways. In the case of beta-lactamase signal protein, cases have been shown of successful fusion proteins secreted by the beta-lactamase signal protein (link to: http://www.ncbi.nlm.nih.gov/pubmed/6607473). Successful continual excretion of the mycotoxin degradation enzymes can be purified in mass amounts periodically, or the chimeric E. Coli can be placed directly on crops to prevent </div>
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| - | SPONSORS
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