Template:Team:TPHS/Main

From 2014hs.igem.org

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<!--<html>
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{{Team:TPHS/ContentFix}}
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<head>
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<html>
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<!-- Reset for BodyContent -->
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<head>
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<style>
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<meta charset="utf-8">
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#content{
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<title>iGEM San Diego</title>
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width: 100%;
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<script src="http://code.jquery.com/jquery-1.11.1.min.js"></script>
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margin: 0;
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<script>
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padding: 0;
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$(function() {
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background-color:transparent;
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  $('a[href*=#]:not([href=#])').click(function() {
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border: none;
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     if (location.pathname.replace(/^\//,'') == this.pathname.replace(/^\//,'') && location.hostname == this.hostname) {
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}
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      var target = $(this.hash);
-
+
      target = target.length ? target : $('[name=' + this.hash.slice(1) +']');
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#bodyContent{
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      if (target.length) {
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width: 100%;
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        $('html,body').animate({
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margin: 0;
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          scrollTop: target.offset().top
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padding: 0;
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        }, 1000);
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background-color:transparent;
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        return false;
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border: none;
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      }
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}
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     }
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</style>
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  });
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+
});
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<!-- Fonts -->
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</script>
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<link href='http://fonts.googleapis.com/css?family=Raleway:400,500,600' rel='stylesheet' type='text/css'>
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-
<link href='http://fonts.googleapis.com/css?family=Antic+Slab' rel='stylesheet' type='text/css'>
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-
+
-
<!-- CSS for Browser Reset-->
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-
<style>
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-
#top-section{
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-
height: 25px;
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-
margin-bottom: 0;
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border: none;
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-
background: #737373;
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width: 100%;
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position: fixed;
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top: 0;
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z-index: 10;
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}
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-
 
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#header {
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width: 1050px;
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text-align: left;
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margin-left: auto;
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margin-right: auto;
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margin-bottom: 0;
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}
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+
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#p-logo {
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display: none;
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}
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#search-controls {
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overflow:hidden;
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display:none;
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background: none;
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position: absolute;
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top: 170px;
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right: 40px;
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}
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#searchform {
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     display: none;
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}
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.left-menu, .right-menu{
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position: absolute;
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background: none;
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-
color: black;
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margin-top: 4px;
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}
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-
 
+
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.left-menu a, .left-menu li {
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    color: #000000;
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}
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.left-menu ul:hover a {
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color: #000000;
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}
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.left-menu ul li, .right-menu ul li a {
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background: none;
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color: #000000;
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-
}
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-
 
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.left-menu li a:hover, .right-menu li a:hover, .left-menu li:hover, .right-menu li:hover {
+
-
     color: #000000;
+
-
}
+
-
 
+
-
#top-section div#menubar.left-menu ul li:nth-child(7) {
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display: none;
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}
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-
 
+
-
.left-menu ul:hover, .right-menu ul:hover {
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background: #737373;
+
-
color: #000000;
+
-
}
+
 +
<!--<script>
 +
$(document).ready(function() { 
 +
var stickyNavTop = $('.nav').offset().top; 
 +
 
 +
var stickyNav = function(){ 
 +
var scrollTop = $(window).scrollTop(); 
 +
     
 +
if (scrollTop > stickyNavTop) { 
 +
    $('.nav').addClass('sticky'); 
 +
} else { 
 +
    $('.nav').removeClass('sticky'); 
 +
} 
 +
}; 
 +
 
 +
stickyNav(); 
 +
 
 +
$(window).scroll(function() { 
 +
    stickyNav(); 
 +
}); 
 +
}); 
 +
</script>-->
 +
<style>
#content{
#content{
-
margin-top: -13px;
+
width: 100%;
-
padding: 0;
+
-
border: 0;
+
-
}
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-
 
+
-
.firstHeading {
+
-
visibility:hidden;
+
-
display:none;
+
-
}
+
-
 
+
-
#catlinks{
+
-
display:none;
+
-
}
+
-
 
+
-
#menubar ul:hover {
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-
color: transparent;
+
-
}
+
-
/*important for background colours*/
+
-
.mediawiki{
+
-
background: #ffffff;
+
-
}
+
-
 
+
-
#footer-box {
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-
display: none;
+
-
}
+
-
/*End minimal header*/
+
-
 
+
-
/* reset browser styles */
+
-
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, p, blockquote, pre, a, abbr, acronym,
+
-
address, big, cite, code, del, dfn, em, img, ins, kbd, q, s, samp,small, strike, strong, sub, sup,
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tt, var, b, u, i, center, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot,
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thead, tr, th, td, article, aside, canvas, details, embed, figure, figcaption, footer, header, hgroup, menu,
+
-
nav, output, ruby, section, summary, time, mark, audio, video {
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margin: 0;
margin: 0;
padding: 0;
padding: 0;
-
border: 0;
+
background-color:transparent;
-
font-size: 16px;
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border: none;
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vertical-align: baseline;
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}
-
}
+
-
 
+
body{
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article, aside, details, figcaption, figure, footer, header, hgroup, menu, nav, section {
+
-
display: block;
+
-
}
+
-
 
+
-
body {
+
-
line-height: 1.2;
+
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}
+
-
 
+
-
table {
+
-
border-collapse: collapse;
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border-spacing: 0;
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}
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ol {
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padding-left: 1.4em;
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list-style: decimal;
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}
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+
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ul {
+
-
padding-left: 1.4em;
+
-
list-style: square;
+
-
}
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+
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blockquote, q {
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quotes: none;
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}
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+
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blockquote:before, blockquote:after, q:before, q:after {
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content: '';
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content: none;
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}
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* {
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box-sizing: border-box;
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-moz-box-sizing: border-box;
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}
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/* end reset browser styles */
+
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+
-
</style>
+
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+
-
<!-- CSS for Navigation Bar-->
+
-
<style>
+
-
#TopBar {
+
-
background: #590E13;
+
width: 100%;
width: 100%;
-
min-width: 762px;
+
margin: 0;
-
height: 89px;
+
-
z-index: 10;
+
-
position: fixed;
+
-
text-align: center;
+
-
top: 25px;
+
-
}
+
-
 
+
-
#TopBar #TopLvlNavLink {
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-
display: none;
+
-
}
+
-
 
+
-
#TopBar ul {
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-
background: #590E13;
+
-
list-style: none;
+
-
position: relative;
+
-
display: inline-table;
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padding: 0;
padding: 0;
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margin: 0;
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background-color:none;
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}
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border: none;
-
 
+
}
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#TopBar ul:after {
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#globalwrapper{
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content: "";
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-
clear: both;
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-
display: block;
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-
}
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-
 
+
-
#TopBar li {
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float: left;
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width: 124px;
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-
text-align: center;
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}
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-
 
+
-
#TopBar li:hover > a {
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text-decoration: underline;
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-
}
+
-
 
+
-
#TopBar a {
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-
display: block;
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width: 100%;
width: 100%;
height: 100%;
height: 100%;
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padding: 15px 0;
+
background: none;
-
color: #FFFFFF;
+
-
text-decoration: none;
+
-
font: 24px Raleway, Arial, san-serif;
+
-
}
+
-
 
+
-
#TopBar > #TopLvlNav  > li > a {
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-
padding: 29px 0;
+
-
}
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-
 
+
-
#TopBar ul ul {
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-
display: none;
+
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background: #590E13;
+
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padding: 0 10px;
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position: absolute;
+
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top: 95%;
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z-index: 10;
+
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margin-left: -10px;
+
-
}
+
-
 
+
-
#TopBar ul li:hover > ul {
+
-
display: block;
+
-
}
+
-
 
+
-
#TopBar ul ul li {
+
-
float: none;
+
-
position: relative;
+
-
}
+
-
 
+
-
#TopBar ul ul a {
+
-
font-size: 18px;
+
-
}
+
-
 
+
-
#TopBar ul ul ul {
+
-
position: absolute;
+
-
left: 100%;
+
-
top: 0;
+
-
margin: 0;
+
-
}
+
-
 
+
-
@keyframes rotate {
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-
  from {
+
-
    transform: rotate(0deg);
+
-
    -ms-transform: rotate(0deg);
+
-
  }
+
-
  to {
+
-
    transform: rotate(59deg);
+
-
    -ms-transform: rotate(59deg);
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-
  }
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}
+
-
 
+
-
@-webkit-keyframes rotate {
+
-
  from {
+
-
    -webkit-transform: rotate(0deg);
+
-
  }
+
-
  to {
+
-
    -webkit-transform: rotate(59deg);
+
-
  }
+
-
}
+
-
 
+
-
@-moz-keyframes rotate {
+
-
  from {
+
-
    transform: rotate(0deg);
+
-
  }
+
-
  to {
+
-
    transform: rotate(59deg);
+
-
  }
+
-
}
+
-
 
+
-
@-o-keyframes rotate {
+
-
  from {
+
-
    transform: rotate(0deg);
+
-
  }
+
-
  to {
+
-
    transform: rotate(59deg);
+
-
  }
+
-
}
+
-
 
+
-
#TopBar #LogoItem:hover > img {
+
-
    -webkit-animation-name:            rotate;
+
-
    -webkit-animation-duration:        .5s;
+
-
    -webkit-animation-iteration-count:  infinite;
+
-
    -webkit-animation-timing-function: linear;
+
-
}
+
-
 
+
-
#TopBar #LogoItem {
+
-
padding: 8px 0;
+
-
}
+
-
 
+
-
#TopBarCollapse {
+
-
position: fixed;
+
-
width: 120px;
+
-
height: 60px;
+
-
top: 25px;
+
-
background: orange;
+
-
z-index: 10;
+
-
}
+
-
 
+
-
#TopBarCollapse #TopLvlNavLink {
+
-
display: block;
+
-
width: 100%;
+
-
height: 100%;
+
-
}
+
-
 
+
-
#TopBarCollapse:after {
+
-
content: "";
+
-
clear: both;
+
-
display: block;
+
-
}
+
-
 
+
-
#TopBarCollapse ul {
+
-
display: none;
+
-
list-style: none;
+
-
padding: 0;
+
-
}
+
-
 
+
-
#TopBarCollapse ul:after {
+
-
content: "";
+
-
clear: both;
+
-
display: block;
+
-
}
+
-
 
+
-
#TopBarCollapse li {
+
-
float: left;
+
-
display: block;
+
-
clear: both;
+
-
width: 120px;
+
-
padding: 0;
+
-
background: green;
+
-
}
+
-
 
+
-
#TopBarCollapse li:hover {
+
-
background: pink;
+
-
}
+
-
 
+
-
#TopBarCollapse a {
+
-
color: #FFFFFF;
+
-
display: block;
+
-
text-decoration: none;
+
-
padding: 10px 15px;
+
-
}
+
-
 
+
-
#TopBarCollapse #LogoItem {
+
-
display: none !important;
+
-
}</style>
+
-
+
-
<!-- CSS for Content -->
+
-
 
+
-
<style>
+
-
 
+
-
 
+
-
html{
+
-
background-image:url('http://fc04.deviantart.net/fs71/f/2011/293/0/1/dark_textured_background_6_by_dianascreations-d4dgfhb.jpg');
+
}
}
 +
#contentSub, #search-controls, .firstHeading, #footer-box, #catlinks, #p-logo {
 +
    display:none;}
 +
#top-section {
 +
    border: none;
 +
    height: 0px;}
 +
#content {
 +
    border: none;}
 +
/* Removes "teams" from the menubar */
 +
#menubar > ul > li:last-child {
 +
    display: none;}
 +
/* Resizes the menubar to fik the links (default is 400px) */
 +
#menubar {
 +
    width: auto;}
body {
body {
-
min-width: 766px;
+
    margin: 10px 0 0 0;
-
}
+
    padding: 0;}
-
 
+
#top-section {
-
#globalWrapper {
+
     width: 965px;
-
padding-bottom: 0;
+
    height: 0;
-
}
+
    margin: 0 auto;
-
 
+
    padding: 0;
-
#bodyContent {
+
    border: none;}
-
background-image:url('http://fc04.deviantart.net/fs71/f/2011/293/0/1/dark_textured_background_6_by_dianascreations-d4dgfhb.jpg');
+
#menubar {
-
text-align: center;
+
    font-size: 65%;
-
}
+
    top: -14px;}
-
 
+
.left-menu:hover {
-
#contentSub{
+
    background-color: transparent;}
-
     display: none;
+
#menubar li a {
-
}
+
    background-color: transparent;}
-
 
+
#menubar:hover {
-
 
+
    color: white;}
-
 
+
#menubar li a {
-
#Banner h1 {
+
    color: transparent;}
-
font: 72px/180px Raleway, Arial, sans-serif;
+
#menubar:hover li a {
-
font-weight: 900;
+
    color: white;}
-
color: #231F20;
+
-
}
+
-
 
+
-
#Banner i {
+
-
font-size: 72px;
+
-
}
+
-
 
+
-
#Content {
+
-
position: relative;
+
-
top: 108px;
+
-
height: 100%;
+
-
width: 100%;
+
-
max-width: 1200px;
+
-
background: #E7E7E7;
+
-
display: inline-block;
+
-
padding: 30px 50px;
+
-
box-shadow: 0px 8px 10px #888, -10px 8px 15px #888, 10px 8px 15px #888;
+
-
margin-bottom: 20px;
+
-
}
+
-
 
+
-
#Content img {
+
-
margin: 4px 20px 12px 20px;
+
-
}
+
-
 
+
-
#Content h1, #Content h2, #Content h3, #Content h4, #Content h5, #Content h6 {
+
-
font-family: 'Raleway', Helvetica, Arial, sans-serif;
+
-
line-height: 1.6;
+
-
text-align: left;
+
-
}
+
-
 
+
-
#Content h1 {
+
-
font-size: 35px;
+
-
font-weight: 700;
+
-
border-bottom: 3px solid #5A5A5A;
+
-
color: #111111;
+
-
margin: 0 -20px 30px -20px;
+
-
padding-left: 20px;
+
-
}
+
-
 
+
-
#Content h1 > i, #Content h1 > em {
+
-
font-size: 35px;
+
-
}
+
-
 
+
-
#Content h2 {
+
-
font-size: 28px;
+
-
font-weight: 600;
+
-
color: #333333;
+
-
margin-top: 30px;
+
-
}
+
-
 
+
-
#Content h2 > i, #Content h2 > em {
+
-
font-size: 28px;
+
-
}
+
-
 
+
-
#Content h3 {
+
-
font-size: 26px;
+
-
font-weight: 500;
+
-
color: #333333;
+
-
padding-left: 20px;
+
-
}
+
-
 
+
-
#Content h4 {
+
-
font-size: 20px;
+
-
font-weight: 500;
+
-
color: #333333;
+
-
padding-left: 20px;
+
-
}
+
-
 
+
-
#Content h3 > i, #Content h3 > em {
+
-
font-size: 26px;
+
-
}
+
-
 
+
-
#Content p {
+
-
font: 18px/1.6 'Segoe UI', Calibri, Helvetica, sans-serif;
+
-
margin: 4px 0 12px 0;
+
-
padding-left: 20px;
+
-
text-indent:55px;
+
-
color: #000000;
+
-
text-align:justify;
+
-
text-justify:inter-word;
+
-
}
+
-
 
+
-
#Content a {
+
-
font: 18px/1.6 'Segoe UI', Calibri, Helvetica, sans-serif;
+
-
}
+
-
 
+
-
#Content i, #Content em, #Content b, #Content strong {
+
-
font-size: 18px;
+
-
}
+
-
 
+
-
#Content li a {
+
-
line-height: 1;
+
-
}
+
-
 
+
-
#Content sub {
+
-
vertical-align: sub;
+
-
font-size: 69%;
+
-
}
+
-
 
+
-
#Content sup {
+
-
vertical-align: super;
+
-
font-size: 69%;
+
-
}
+
-
 
+
-
#Content span {
+
-
font-size: inherit;
+
-
}
+
-
 
+
-
#Content .noIndent {
+
-
text-indent: 0;
+
-
}
+
-
 
+
-
#Content .Blue, #Content .blue {
+
-
color: #299D7F;
+
-
}
+
-
 
+
-
#Content .Green, #Content .green {
+
-
color: #87CA7A;
+
-
}
+
-
 
+
-
#Content .Orange, #Content .orange {
+
-
color: #FF5900;
+
-
}
+
-
 
+
-
#Content .Yellow, #Content .yellow {
+
-
color: #EDB74B;
+
-
}
+
-
 
+
-
#Content ul, #Content ol {
+
-
padding-left: 40px;
+
-
}
+
-
 
+
-
#Content li {
+
-
font: 18px/1 'Segoe UI', Calibri, Helvetica, sans-serif;
+
-
margin: 4px 0 12px 0;
+
-
color: #000000;
+
-
text-align:justify;
+
-
text-justify:inter-word;
+
-
}
+
-
 
+
-
#Content table {
+
-
background: none;
+
-
margin: 4px 20px 12px 20px;
+
-
}
+
-
 
+
-
#Content td {
+
-
border: 1px solid #000000;
+
-
padding: 5px;
+
-
}
+
-
 
+
-
#Content figure {
+
-
margin-bottom: 20px;
+
-
}
+
-
 
+
-
#Content figcaption {
+
-
padding: 0 50px;
+
-
}
+
-
 
+
-
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        STATISTICS<br />
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    <span style="font-family: Lato; font-size: 18px;">Statistics that are quite relevant to the nature of this experiment</span>
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            Toxicity of aflatoxin is 10 times that of hydrocyanic acid and 68 times of arsenic.
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      <img src="https://static.igem.org/mediawiki/2014/e/e4/Globe.png" width="150px" height="150px" /><br />
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            This disease is the third-leading cause of cancer death globally according to WHO (2008), with about 550,000–600,000 new cases each year.
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            This disease is the third-leading cause of cancer death globally according to WHO (2008), with about 550,000–600,000 new cases each year
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        ENGINEERING<br />
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    <span style="font-family: 'Lato'; font-size: 18px;">E. Coli Capable of Extracelluar Secretion of Mycotoxin Detoxifying Enzymes</span>
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    <div id="third-text">      Microfungi that produce harmful mycotoxins flourish on improperly-stored nuts, grains, meat, and dairy. They especially thrive in developing countries, where the lack of advanced food storage and mycotoxin exposure causes 40% of the diseases. To lessen the problem, our team engineered E. coli strains using synthetic biology tools to produce chimeric mycotoxin-degrading fungal enzymes, Aflatoxin-Detoxifizyme (ADTZ) and Zearalenone Hydrolase (ZHD101), which are designed to be secreted to extra-cellular space by fusing with secretion signal peptides from alpha-amylase and beta-lactamase. In this study, we have successfully generated synthetic genetic materials to produce four chimeric mycotoxin-detoxifying enzymes. The levels of extracellular secretion is also characterized and analyzed. The project will allow a mass production of detoxification enzymes in cost effective way, preventing the squandering of harvested crops, and limiting mycotoxin-related diseases. Increased access to these proteins will have an immense commercial, industrial, agricultural, and health impact.</div> 
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Latest revision as of 08:09, 20 June 2014

iGEM San Diego

STATISTICS
Statistics that are quite relevant to the nature of this experiment

Toxicity of aflatoxin is 10 times that of hydrocyanic acid and 68 times of arsenic.

This disease is the third-leading cause of cancer death globally according to WHO (2008), with about 550,000–600,000 new cases each year.

This disease is the third-leading cause of cancer death globally according to WHO (2008), with about 550,000–600,000 new cases each year
ENGINEERING
E. Coli Capable of Extracelluar Secretion of Mycotoxin Detoxifying Enzymes
Microfungi that produce harmful mycotoxins flourish on improperly-stored nuts, grains, meat, and dairy. They especially thrive in developing countries, where the lack of advanced food storage and mycotoxin exposure causes 40% of the diseases. To lessen the problem, our team engineered E. coli strains using synthetic biology tools to produce chimeric mycotoxin-degrading fungal enzymes, Aflatoxin-Detoxifizyme (ADTZ) and Zearalenone Hydrolase (ZHD101), which are designed to be secreted to extra-cellular space by fusing with secretion signal peptides from alpha-amylase and beta-lactamase. In this study, we have successfully generated synthetic genetic materials to produce four chimeric mycotoxin-detoxifying enzymes. The levels of extracellular secretion is also characterized and analyzed. The project will allow a mass production of detoxification enzymes in cost effective way, preventing the squandering of harvested crops, and limiting mycotoxin-related diseases. Increased access to these proteins will have an immense commercial, industrial, agricultural, and health impact.

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