Team:CIDEB-UANL Mexico/labwork futureplans

The next step to follow in order to accomplish the E.CARU project, must be to put the four pieces in which the team has been working (nhaS, irrE, BSTM1 opt. and L2+AIDA) into a single E.coli. As it was proposed at the beginning E. coli would contain two plasmids: one with the circuit composed by IrrE and L2+AIDA, and the other by NhaS and BSMT1 opt.; both with different antibiotics in order to know that both are inside the cell.

It is expected that someday in the future, the theoretical design that was exposed in the abstract of our project can be finished. It means that all the four modules that conform E.CARU can be put together, not only as four separated pieces (see figure 1), but all together: BSTM1 opt. in front of NhaS as a reporter, and irrE with L2+AIDA in another plasmid, in order to give to E. coli resistance and the ability for binding silica respectively. Then, it could be used as the bio-filter proposed, and in a future used in a water treatment plant for processing saline water into usable or even potable water.