Team:FHS Frederick MD

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{{:Team:FHS_Frederick_MD/Header}}
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=Overview=
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We are interested in creating a [[Team:FHS_Frederick_MD/Microbial_Fuel_Cells|microbial fuel cell]] that utilizes the facultative anaerobic bacteria, ''Shewanella oneidensis'', to produce [[Team:FHS_Frederick_MD/Renewable_Energy|renewable energy]] and [[Team:FHS_Frederick_MD/Clean_Water|clean water]].  
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In order to optimize the growth conditions in the anaerobic chamber of the fuel cell, a fluorescent protein marker will be added so to visualize bacterial growth under different conditions.   
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We plan to accomplish this using the [[Team:FHS_Frederick_MD/NirB_Promoter|NirB oxygen-sensitive promoter]] to induce expression of the glowing gene only when oxygen is scarce.  Furthermore, we must engineer a fluorescent [[Team:FHS_Frederick_MD/LOV_Domain|LOV domain]], which is optimized for expression in ''S. oneidensis'' and capable of fluorescence under anaerobic conditions. 
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This genetic construct will help us ensure that the bacteria are actually growing under anaerobic conditions.  This would lead to the creation of a BioBrick that can be deposited back into the “toolbox” parts repository for future iGEM teams.
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{{:Team:FHS_Frederick_MD/Footer}}
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|align="center"|[[Team:FHS_Frederick_MD | Team FHS_Frederick_MD]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=FHS_Frederick_MD Official Team Profile]
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===Team===
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We are interested in creating a microbial fuel cell that utilizes anaerobic bacteria to produce electricity.  In order to optimize the growth conditions in the fuel cell, a fluorescent protein marker will be added so to visualize bacterial growth.  We plan to implement an oxygen-sensitive promoter to induce expression of the glowing gene.  This should ensure that bacteria only grow under anaerobic conditions.  This would lead to the creation of a genetic construct that can be deposited back into the “toolbox” parts repository for iGEM.
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===Goals===
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===Microbial Fuel Cells===
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This is about microbial fuel cells.
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(Alan's section)
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A microbial fuel cell is a device that converts the chemical reactions of bacteria into electricity. Within the fuel cell certain bacteria under anaerobic conditions will remove the electrons from organic matter and transfer them to an anode, which will then transfer the electrons through a circuit to a cathode. The current and voltage produced by this process is what creates the electricity required to power certain objects such as a light bulb.
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The bacteria that we are currently creating is meant to optimize the microbial fuel cell's potential, as bacteria that will glow under anaerobic conditions will reveal any weaknesses in the fuel cell, structural or otherwise, which can then be assessed and dealt with.
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===Gene Design===
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(Kyle and Jonathon, briefly summarize how NirB and LOV work together.)
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====NirB Promoter====
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(Kyle, can you add a paragraph here on the NirB promoter?)
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====LOV Domain====
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(Jonathon, this is your area to describe how we engineered the LOV gene.)
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===Methods===
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(This is Dillon's domain.)
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====3A Assembly====
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We used the 3A, or 3 antibody, assembly kit in order to transform E.coli with two genes, the LOV gene and the NirB gene.  These genes  will allow for further work with Schwenella bacteria in the anaerobic microbial fuel cell.  We then used the mini-prep components of the kit to purify our plasmid. We verified the plasmid's presence through electrophoresis and further  sequence analysis.
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===Notebook===
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Show us how you spent your days.
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===Results/Conclusions===
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What did you achieve over the course of your semester?
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===Safety===
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What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
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===Attributions===
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(ALL: Please write something about yourself and what you have been doing on the team.)
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|[[Image:KyleA.jpg|100px]]
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|style="vertical-align:top;"|'''Kyle Andrushko''' ...
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|[[Image:DillonK.jpg|100px]]
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|style="vertical-align:top;"|'''Dillon Kestner''' ...
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|[[Image:JonathonS.jpg|100px]]
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|style="vertical-align:top;"|'''Jonathon Soward''' ...
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|[[File:AlanN.jpg|100px]]
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|style="vertical-align:top;"|'''Alan Nguyen''' ...
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|[[Image:MarkT.jpg|100px]]
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|style="vertical-align:top;"|'''Mark Trice''' is a teacher at Frederick High School, where he teachers classes such as Chemistry, Physics, Biology, and Advanced Placement Biology.  He also serves as the vice president on the board for the nonprofit organization, Ars Biotechnica.  Mark has worked with his iGEM club for 2 years and is so excited to see this project come to fruition.
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|[[Image:DaveR.jpg|100px]]
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|style="vertical-align:top;"|'''David Rozak''' is a research scientist at the United States Army Medical Research Institute for Infectious Diseases and a founding director of the nonprofit organization, Ars Biotechnica, Inc.  Dave has been working with the Frederick High School Bioengineering Club for two years and served as an advisor to our iGEM team.
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|[[File:GaryL.jpg|100px]]
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|style="vertical-align:top;"|'''Gary Lopez''' ...
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<forum_subtle />
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===Human Practices===
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What impact does/will your project have on the public?
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====Renewable Energy====
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(Kyle: How do fuel cell help produce clean renewable energy?)
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====Clean Water====
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(Alan: How do fuel cell produce a clean source of water?)
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====Basic Research====
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(Dillon: How will the oxygen-sensitive fluorescent gene we're making help people study and improve upon microbial fuel cell performance?)
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====Public Awareness====
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(Jonathon: Discuss how our meeting with Cardin helped raise public awareness for STEM.)
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===Fun!===
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What was your favorite team snack?? Have a picture of your team mascot?
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[[File:Tshirts.jpg|500px]]
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[[File:cardin.jpg]]
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<forum_subtle />
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===Sponsors===
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We'd like to thank the following organizations for making this possible.
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Latest revision as of 01:43, 21 June 2014

FHS Logo.png


Overview

Tshirts.jpg

We are interested in creating a microbial fuel cell that utilizes the facultative anaerobic bacteria, Shewanella oneidensis, to produce renewable energy and clean water.

In order to optimize the growth conditions in the anaerobic chamber of the fuel cell, a fluorescent protein marker will be added so to visualize bacterial growth under different conditions.

We plan to accomplish this using the NirB oxygen-sensitive promoter to induce expression of the glowing gene only when oxygen is scarce. Furthermore, we must engineer a fluorescent LOV domain, which is optimized for expression in S. oneidensis and capable of fluorescence under anaerobic conditions.

This genetic construct will help us ensure that the bacteria are actually growing under anaerobic conditions. This would lead to the creation of a BioBrick that can be deposited back into the “toolbox” parts repository for future iGEM teams.

IGEM-logo.png


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