Team:CIDEB-UANL Mexico/project resistance

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      <h2>Resistance Module</h2>
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<p>Because our project is a biofilter which allows to remove salt from water, the bacteria needs to survive to extreme environmental conditions which normally it can’t do. We need <i>E. Coli</i> to survive a high salinity environment to allow it to capture sodium ions and to remove the salt concentration of the water.</p><p>In order to accomplish our project goal, we have to change <i>E. Coli</i> metabolism and make it stronger, more resistant and more efficient than in normal <i>E.coli</i> bacteria.</p>
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<p>Because our project is a biofilter which allows to remove salt from water, the bacteria needs to survive to extreme environmental conditions which normally it can’t do. We need <i>E. coli</i> to survive a high salinity environment to allow it to capture sodium ions and to remove the salt concentration of the water.</p>
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<p>In order to accomplish our project goal, we have to change <i>E. coli</i> metabolism and make it stronger, more resistant and more efficient than in normal <i>E. coli</i> bacteria.</p>
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<td style="padding-left:12px;"><img width=131 height=127 src="https://static.igem.org/mediawiki/2014hs/8/8e/BeutifulResistance.jpg"/>
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<td style="padding-left:12px;"><img width=131 height=140 src="https://static.igem.org/mediawiki/2014hs/8/8e/BeutifulResistance.jpg"/>
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<p>Last discoveries show irrE as a protein capable of change the <i>E. Coli</i> metabolism, and giving it the ability to survive to bigger temperatures, bigger UV rays radiation and bigger salt concentration (UCL, 2012).</p>
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<p>Last discoveries show irrE as a protein capable of change the <i>E. coli</i> metabolism, and giving it the ability to survive to bigger temperatures, bigger UV rays radiation and bigger salt concentration (UCL, 2012).</p>
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<br>
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<p><b><h2>Description</h2></b></p>  
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<br><p><b>What does irrE do?</b></p>
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<p>The protein irrE originates from <i>Deinococcus radiodurans</i>, and initially, this gene provides resistance to radiation. But when transformed in <i>E. coli</i>, it protects it against salt, oxidative and thermal shock. (UCL, 2012) Also, different experiments from different iGEM teams, support the idea of the bigger salt resistance in <i>E. coli</i> with this biobrick. </p><center>
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<p>The protein irrE originates from <i>Deinococcusradiodurans</i>, and initially, this gene provides resistance to radiation. But when transformed in <i>E. Coli</i>, it protects it against salt, oxidative and thermal shock. (UCL, 2012) Also, different experiments from different iGEM teams, support the idea of the bigger salt resistance in <i>E. Coli</i> with this biobrick. </p>
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<p><img width=564 height=350 src="https://static.igem.org/mediawiki/2014hs/3/3e/Grafica_1_cideb2014.png"align=center hspace=12 alt="IMG_0317"></p>
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<center><p><img width=564 height=350 src="https://static.igem.org/mediawiki/2014hs/3/3e/Grafica_1_cideb2014.png"
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<p><b>Image 1.</b> This graph done by <a herf="https://2012.igem.org/Team:University_College_London">UCL iGEM 2012</a>, shows how irrE biobrick increased the salt concentration resistance in <i>E. coli</i> compared with the results from <a href="https://2010.igem.org/Team:TU_Delft">Tu Delf iGEM 2010</a>.</p></center>
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align=center hspace=12 alt="IMG_0317"></p>
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<br>
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<p><b>Image 1.</b> This graph done by <a herf="https://2012.igem.org/Team:University_College_London">UCL iGEM 2012</a>, shows how irrE biobrick increased the salt concentration resistance in <i>E. Coli</i> compared with the results from <a href="https://2010.igem.org/Team:TU_Delft">Tu Delf iGEM 2010</a>.</p></center>
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<p>irrE has been demonstrated to up regulate transcription of recA and pprA - genes which encode Recombines A and Radiation Inducible Protein. With respect to salt tolerance, irrE up regulates the production of several stress responsive proteins, protein kinases, metabolic proteins, and detoxification proteins. It also down regulates glycerol degradation. With this global regulatory effect, <i>E. coli</i> becomes more salt tolerant (UCL, 2012).</p><center>
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<br><p><b>How it works?</b></p>
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<p><img width=429 height=322 src="https://static.igem.org/mediawiki/2014hs/9/9b/Imagen1cideb2014.png"align=center hspace=12 alt="IMG_0317"></p>
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<p>irrE has been demonstrated to up regulate transcription of recA and pprA - genes which encode Recombines A and Radiation Inducible Protein. With respect to salt tolerance, irrE up regulates the production of several stress responsive proteins, protein kinases, metabolic proteins, and detoxification proteins. It also down regulates glycerol degradation. With this global regulatory effect, <i>E. Coli</i> becomes more salt tolerant (UCL, 2012).</p>
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<p><b>Image 2.</b> This diagram shows the effect of irrE protein on <i>E. coli</i> metabolism. </p></center>
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<p><b><h2>Device</h2></b></p>
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<center><p><img width=429 height=322 src="https://static.igem.org/mediawiki/2014hs/9/9b/Imagen1cideb2014.png"
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<p>Initially, IrrE and L2+AIDA, protein for binding silica (<a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_union">Union module</a>), were joined together in only one circuit, but we needed to separate them because L2+AIDA has not been proved yet and it could affect the correct production of IrrE (see image 3).</p><center>
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align=center hspace=12 alt="IMG_0317"></p>
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<p><b>Image 2.</b> This diagram shows the effect of irrE protein on <i>E. Coli</i> metabolism. </p></center>
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<p><img width=529 height=322 src="https://static.igem.org/mediawiki/2014hs/0/05/Circuit_l2_and_irre.png"align=center hspace=12 alt="IMG_0317"></p>
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<p><b>How it is composed?</b></p>
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<p><b>Image 3.</b> Circuit for our project and for testing resistance and union modules. </p></center>
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<br>
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<p>Initially, IrrE and L2+AIDA, protein for binding silica (<a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_union">Union module </a>), were joined together in only one circuit, but we needed to separate them becauseL2+AIDA has not been proved yet and it could affect the correct production of IrrE (see image 3).</p>
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<p><b><h2>Parts of the Module</h2></b></p>
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<br><center>
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<p><b>Image 3.</b> Circuit for our project and for testing resistance and union modules. </p></center>
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<br><p><b>Other teams that used it:</b></p>
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</span></b><b style='mso-bidi-font-weight:normal'>
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<p><b><a href="https://2012.igem.org/Team:University_College_London">UCL 2012</a>:</b> They propose to confer salt tolerance on <i>E. Coli</i> by linking the salt tolerance gene encoding the protein irrE (<a href="http://parts.igem.org/Part:BBa_K729001">BBa_K729001</a>) to a constitutive promoter (<a href="http://parts.igem.org/Part:BBa_J23119">BBa_J23119</a>). </p>
+
<span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:"Times New Roman";mso-fareast-language: ES-MX;mso-bidi-font-style:italic'><o:p></o:p>
 +
</span></b></p>  
 +
</td>  
 +
<td width=119 nowrap valign=top style='width:89.0pt;border:none;border-bottom: solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint: 153;mso-border-bottom-alt:solid #FABF8F .5pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor: background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'>  
-
<center><p><img width=661 height=139 src="https://static.igem.org/mediawiki/2014hs/3/33/Gen1cideb2014.png"
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:1'><b>
-
align=center hspace=12 alt="IMG_0317"></p>
+
-
<p><b>Image 3.</b> Part designed by <a href="https://2012.igem.org/Team:University_College_London">UCL 2012</a> for the irrE protein.</p></center>
+
<span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX'>CODE
 +
</span></b><b style='mso-bidi-font-weight:normal'>
-
<br><p><b>irrE's parts description :</b></p><br>
+
<span lang=ES-MX style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: "Times New Roman";mso-fareast-language:ES-MX'><o:p></o:p>
 +
</span></b></p>  
 +
</td>
 +
<td width=400 nowrap valign=top style='width:300.0pt;border:none;border-bottom: solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint: 153;mso-border-bottom-alt:solid #FABF8F .5pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor: background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'>  
-
<center><div>
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:1'><b>
-
<table class=MsoTable15Grid7ColorfulAccent6 border=1 cellspacing=0
+
 
-
cellpadding=0 width=631 style='width:473.0pt;border-collapse:collapse;
+
<span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX'>DESCRIPTION
-
border:none;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6;
+
</span></b><b style='mso-bidi-font-weight:normal'>
-
mso-border-themetint:153;mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+
 
-
<tr style='mso-yfti-irow:-1;mso-yfti-firstrow:yes;mso-yfti-lastfirstrow:yes;
+
<span lang=ES-MX style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: "Times New Roman";mso-fareast-language:ES-MX'><o:p></o:p>
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  height:15.75pt'>
+
</span></b></p>  
-
  <td width=112 nowrap valign=top style='width:84.0pt;border:none;border-bottom:
+
</td>  
-
  solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint:
+
</tr>  
-
  153;mso-border-bottom-alt:solid #FABF8F .5pt;mso-border-bottom-themecolor:
+
<tr style='mso-yfti-irow:0;height:60.75pt'>  
-
  accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor:
+
<td width=112 nowrap valign=top style='width:84.0pt;border:none;border-right: solid #FABF8F 1.0pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; height:60.75pt'>  
-
  background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'>
+
 
-
  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+
<p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:68'>
-
  text-align:center;line-height:normal;mso-yfti-cnfc:517'><b><span lang=ES-MX
+
<!--[if gte vml 1]><v:shapetype id="_x0000_t75" coordsize="21600,21600" o:spt="75" o:preferrelative="t" path="m@4@5l@4@11@9@11@9@5xe" filled="f" stroked="f"> <v:stroke joinstyle="miter"/> <v:formulas> <v:f eqn="if lineDrawn pixelLineWidth 0"/> <v:f eqn="sum @0 1 0"/> <v:f eqn="sum 0 0 @1"/> <v:f eqn="prod @2 1 2"/> <v:f eqn="prod @3 21600 pixelWidth"/> <v:f eqn="prod @3 21600 pixelHeight"/> <v:f eqn="sum @0 0 1"/> <v:f eqn="prod @6 1 2"/> <v:f eqn="prod @7 21600 pixelWidth"/> <v:f eqn="sum @8 21600 0"/> <v:f eqn="prod @7 21600 pixelHeight"/> <v:f eqn="sum @10 21600 0"/> </v:formulas> <v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/> <o:lock v:ext="edit" aspectratio="t"/> </v:shapetype><v:shape id="_x0031__x0020_Imagen" o:spid="_x0000_s1033" type="#_x0000_t75" style='position:absolute;left:0;text-align:left; margin-left:15.9pt;margin-top:9.9pt;width:61.45pt;height:48.6pt;z-index:251649536; visibility:visible;mso-wrap-style:square;mso-width-percent:0; mso-height-percent:0;mso-wrap-distance-left:9pt;mso-wrap-distance-top:0; mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:0; mso-position-horizontal:absolute;mso-position-horizontal-relative:text; mso-position-vertical:absolute;mso-position-vertical-relative:text; mso-width-percent:0;mso-height-percent:0;mso-width-relative:page; mso-height-relative:page'> <v:imagedata src="TablasAromaYResistencia_archivos/image001.png" o:title=""/> <w:wrap type="square"/> </v:shape><![endif]--><![if !vml]><img width=82 height=65 src="https://static.igem.org/mediawiki/2014hs/6/62/Promotor.png" align=left hspace=12 v:shapes="_x0031__x0020_Imagen"><![endif]><i>
-
  style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman";
+
 
-
  mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX;mso-bidi-font-style:
+
<span lang=ES-MX style='font-family:Oxygen;color:#E36C0A;mso-themecolor:accent6;mso-themeshade: 191;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p>
-
  italic'>IMAGE</span></b><b style='mso-bidi-font-weight:normal'><span
+
</span></i></p>  
-
  lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:
+
 
-
  "Times New Roman";mso-bidi-font-family:"Times New Roman";mso-fareast-language:
+
<p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:68'>&nbsp;</p>
-
  ES-MX;mso-bidi-font-style:italic'><o:p></o:p></span></b></p>
+
</td>  
-
  </td>
+
<td width=119 nowrap valign=top style='width:89.0pt;border-top:none; border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; mso-border-right-themecolor:accent6;mso-border-right-themetint:153; mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; background:#FDE9D9;mso-background-themecolor:accent6;mso-background-themetint: 51;padding:0cm 5.4pt 0cm 5.4pt;height:60.75pt'>  
-
  <td width=119 nowrap valign=top style='width:89.0pt;border:none;border-bottom:
+
 
-
  solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint:
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:64'>
-
  153;mso-border-bottom-alt:solid #FABF8F .5pt;mso-border-bottom-themecolor:
+
 
-
  accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor:
+
<span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";color:black;mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_J23119">BBa_J23119</a><o:p></o:p>
-
  background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'>
+
</span></p>  
-
  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+
 
-
  text-align:center;line-height:normal;mso-yfti-cnfc:1'><b><span lang=ES-MX
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:64'>&nbsp;</p>  
-
  style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman";
+
</td>  
-
  mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX'>CODE</span></b><b
+
<td width=400 valign=top style='width:300.0pt;border-top:none;border-left: none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;background:#FDE9D9;mso-background-themecolor:accent6; mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:60.75pt'>  
-
  style='mso-bidi-font-weight:normal'><span lang=ES-MX style='font-size:12.0pt;
+
 
-
  font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:
+
<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:normal;mso-yfti-cnfc:64'>
-
  "Times New Roman";mso-fareast-language:ES-MX'><o:p></o:p></span></b></p>
+
 
-
  </td>
+
<span style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Arial;color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>In the specific case of our bacteria, it helps to continuously transcribing the  
-
  <td width=400 nowrap valign=top style='width:300.0pt;border:none;border-bottom:
+
 
-
  solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint:
+
<span class=SpellE>irrE
-
  153;mso-border-bottom-alt:solid #FABF8F .5pt;mso-border-bottom-themecolor:
+
</span> gene in order to make bacteria resist high concentration of salt.  
-
  accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor:
+
</span>
-
  background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'>
+
 
-
  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+
<span class=SpellE>
-
  text-align:center;line-height:normal;mso-yfti-cnfc:1'><b><span lang=ES-MX
+
 
-
  style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman";
+
<span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:Arial;color:#282828;mso-fareast-language:ES-MX'>This
-
  mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX'>DESCRIPTION</span></b><b
+
</span>
-
  style='mso-bidi-font-weight:normal'><span lang=ES-MX style='font-size:12.0pt;
+
</span>
-
  font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:
+
 
-
  "Times New Roman";mso-fareast-language:ES-MX'><o:p></o:p></span></b></p>
+
<span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Arial;color:#282828;mso-fareast-language: ES-MX'>  
-
  </td>
+
 
-
</tr>
+
<span class=SpellE>promoter
-
<tr style='mso-yfti-irow:0;height:60.75pt'>
+
</span> has a  
-
  <td width=112 nowrap valign=top style='width:84.0pt;border:none;border-right:
+
 
-
  solid #FABF8F 1.0pt;mso-border-right-themecolor:accent6;mso-border-right-themetint:
+
<span class=SpellE>length
-
  153;mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6;
+
</span> of 35  
-
  mso-border-top-themetint:153;mso-border-top-alt:solid #FABF8F .5pt;
+
 
-
  mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt:
+
<span class=SpellE>pb
-
  solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint:
+
</span>(Anderson, 2006).<o:p></o:p>
-
  153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt;
+
</span></p>  
-
  height:60.75pt'>
+
</td>  
-
  <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt;
+
</tr>  
-
  text-align:right;line-height:normal;mso-yfti-cnfc:68'><!--[if gte vml 1]><v:shapetype
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<tr style='mso-yfti-irow:1;height:75.0pt'>  
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  id="_x0000_t75" coordsize="21600,21600" o:spt="75" o:preferrelative="t"
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<td width=112 nowrap valign=top style='width:84.0pt;border-top:solid #FABF8F 1.0pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;border-left: none;border-bottom:none;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; height:75.0pt'>  
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  path="m@4@5l@4@11@9@11@9@5xe" filled="f" stroked="f">
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  <v:stroke joinstyle="miter"/>
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<p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:4'>
-
  <v:formulas>
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<!--[if gte vml 1]><v:shape id="_x0032__x0020_Imagen" o:spid="_x0000_s1032" type="#_x0000_t75" style='position:absolute; left:0;text-align:left;margin-left:23.1pt;margin-top:6.95pt;width:54.25pt; height:49.45pt;z-index:251651584;visibility:visible;mso-wrap-style:square; mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt; mso-wrap-distance-top:0;mso-wrap-distance-right:9pt; mso-wrap-distance-bottom:0;mso-position-horizontal:absolute; mso-position-horizontal-relative:text;mso-position-vertical:absolute; mso-position-vertical-relative:text;mso-width-percent:0; mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'> <v:imagedata src="TablasAromaYResistencia_archivos/image003.png" o:title=""/> <w:wrap type="square"/> </v:shape><![endif]--><![if !vml]><img width=72 height=66 src="https://static.igem.org/mediawiki/2014hs/5/5d/Cds.png" align=left hspace=12 v:shapes="_x0032__x0020_Imagen"><![endif]><i>
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    <v:f eqn="if lineDrawn pixelLineWidth 0"/>
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    <v:f eqn="sum @0 1 0"/>
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<span style='font-family:Oxygen; color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191;mso-ansi-language: EN-US;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p>
-
    <v:f eqn="sum 0 0 @1"/>
+
</span></i></p>  
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    <v:f eqn="prod @2 1 2"/>
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    <v:f eqn="prod @3 21600 pixelWidth"/>
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<p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:4'>&nbsp;</p>  
-
    <v:f eqn="prod @3 21600 pixelHeight"/>
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</td>  
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    <v:f eqn="sum @0 0 1"/>
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<td width=119 nowrap valign=top style='width:89.0pt;border-top:none; border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; mso-border-right-themecolor:accent6;mso-border-right-themetint:153; mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; padding:0cm 5.4pt 0cm 5.4pt;height:75.0pt'>  
-
    <v:f eqn="prod @6 1 2"/>
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-
    <v:f eqn="prod @7 21600 pixelWidth"/>
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<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal'>
-
    <v:f eqn="sum @8 21600 0"/>
+
 
-
    <v:f eqn="prod @7 21600 pixelHeight"/>
+
<span style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: "Times New Roman";color:black;mso-ansi-language:EN-US;mso-fareast-language: ES-MX'><a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a><o:p></o:p>
-
    <v:f eqn="sum @10 21600 0"/>
+
</span></p>  
-
  </v:formulas>
+
 
-
  <v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/>
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal'>&nbsp;</p>  
-
  <o:lock v:ext="edit" aspectratio="t"/>
+
</td>  
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  </v:shapetype><v:shape id="_x0031__x0020_Imagen" o:spid="_x0000_s1033"
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<td width=400 valign=top style='width:300.0pt;border-top:none;border-left: none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;padding:0cm 5.4pt 0cm 5.4pt;height:75.0pt'>  
-
  type="#_x0000_t75" style='position:absolute;left:0;text-align:left;
+
 
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  margin-left:15.9pt;margin-top:9.9pt;width:61.45pt;height:48.6pt;z-index:251649536;
+
<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:normal'>
-
  visibility:visible;mso-wrap-style:square;mso-width-percent:0;
+
 
-
  mso-height-percent:0;mso-wrap-distance-left:9pt;mso-wrap-distance-top:0;
+
<span style='font-size:12.0pt;font-family:Oxygen; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Arial; color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>This specific is  
-
  mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:0;
+
 
-
  mso-position-horizontal:absolute;mso-position-horizontal-relative:text;
+
<span class=SpellE>RBS
-
  mso-position-vertical:absolute;mso-position-vertical-relative:text;
+
</span> based on  
-
  mso-width-percent:0;mso-height-percent:0;mso-width-relative:page;
+
 
-
  mso-height-relative:page'>
+
<span class=SpellE>Elowitz
-
  <v:imagedata src="TablasAromaYResistencia_archivos/image001.png" o:title=""/>
+
</span>  
-
  <w:wrap type="square"/>
+
 
-
  </v:shape><![endif]--><![if !vml]><img width=82 height=65
+
<span class=SpellE>repressilator
-
  src="https://static.igem.org/mediawiki/2014hs/6/62/Promotor.png" align=left hspace=12
+
</span>. It is very common to see in many  
-
  v:shapes="_x0031__x0020_Imagen"><![endif]><i><span lang=ES-MX
+
 
-
  style='font-family:Oxygen;color:#E36C0A;mso-themecolor:accent6;mso-themeshade:
+
<span class=SpellE>iGEM
-
  191;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p></span></i></p>
+
</span> projects.
-
  <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt;
+
 
-
  text-align:right;line-height:normal;mso-yfti-cnfc:68'>&nbsp;</p></td>
+
<span style='mso-spacerun:yes'>  
-
  <td width=119 nowrap valign=top style='width:89.0pt;border-top:none;
+
</span>It has a length of 12  
-
  border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:
+
 
-
  accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;
+
<span class=SpellE>pb  
-
  mso-border-right-themecolor:accent6;mso-border-right-themetint:153;
+
</span>(Mahajan,  
-
  mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6;
+
 
-
  mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt;
+
<span class=SpellE>Marinescu
-
  mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt:
+
</span>, Chow,  
-
  solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153;
+
 
-
  background:#FDE9D9;mso-background-themecolor:accent6;mso-background-themetint:
+
<span class=SpellE>Wissner
-
  51;padding:0cm 5.4pt 0cm 5.4pt;height:60.75pt'>
+
</span>-Gross, &amp; Carr, 2003).<o:p></o:p>
-
  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+
</span></p>  
-
  text-align:center;line-height:normal;mso-yfti-cnfc:64'><span lang=ES-MX
+
</td>  
-
  style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman";
+
</tr>  
-
  mso-bidi-font-family:"Times New Roman";color:black;mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_J23119">BBa_J23119</a><o:p></o:p></span></p>
+
<tr style='mso-yfti-irow:2;height:90.35pt'>  
-
  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+
<td width=112 nowrap valign=top style='width:84.0pt;border-top:solid #FABF8F 1.0pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;border-left: none;border-bottom:none;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; height:90.35pt'>  
-
  text-align:center;line-height:normal;mso-yfti-cnfc:64'>&nbsp;</p>
+
 
-
  </td>
+
<p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:68'>
-
  <td width=400 valign=top style='width:300.0pt;border-top:none;border-left:
+
<!--[if gte vml 1]><v:shape id="_x0033__x0020_Imagen" o:spid="_x0000_s1031" type="#_x0000_t75" style='position:absolute; left:0;text-align:left;margin-left:23.4pt;margin-top:10pt;width:57pt; height:48pt;z-index:251654656;visibility:visible;mso-wrap-style:square; mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt; mso-wrap-distance-top:0;mso-wrap-distance-right:9pt; mso-wrap-distance-bottom:0;mso-position-horizontal:absolute; mso-position-horizontal-relative:text;mso-position-vertical:absolute; mso-position-vertical-relative:text;mso-width-percent:0; mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'> <v:imagedata src="TablasAromaYResistencia_archivos/image005.png" o:title=""/> <w:wrap type="square"/> </v:shape><![endif]--><![if !vml]><img width=76 height=64 src="https://static.igem.org/mediawiki/2014hs/9/97/IrrEResistance.png" align=left hspace=12 v:shapes="_x0033__x0020_Imagen"><![endif]></p>  
-
  none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;
+
</td>  
-
  mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor:
+
<td width=119 nowrap valign=top style='width:89.0pt;border-top:none; border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; mso-border-right-themecolor:accent6;mso-border-right-themetint:153; mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; background:#FDE9D9;mso-background-themecolor:accent6;mso-background-themetint: 51;padding:0cm 5.4pt 0cm 5.4pt;height:90.35pt'>  
-
  accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt;
+
 
-
  mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt:
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:64'>
-
  solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint:
+
 
-
  153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6;
+
<span style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";color:black;mso-ansi-language:EN-US; mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_K729001">BBa_K729001</a><o:p></o:p>
-
  mso-border-themetint:153;background:#FDE9D9;mso-background-themecolor:accent6;
+
</span></p>  
-
  mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:60.75pt'>
+
 
-
  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align:
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:64'>
-
  justify;line-height:normal;mso-yfti-cnfc:64'><span style='font-size:12.0pt;
+
 
-
  font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:
+
<span style='font-size:3.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";color:black;mso-ansi-language:EN-US; mso-fareast-language:ES-MX'><o:p></o:p>
-
  Arial;color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>In
+
</span></p>  
-
  the specific case of our bacteria, it helps to continuously transcribing the <span
+
</td>  
-
  class=SpellE>irrE</span> gene in order to make bacteria resist high
+
<td width=400 valign=top style='width:300.0pt;border-top:none;border-left: none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;background:#FDE9D9;mso-background-themecolor:accent6; mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:90.35pt'>  
-
  concentration of salt. </span><span class=SpellE><span lang=ES-MX
+
 
-
  style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman";
+
<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:normal;mso-yfti-cnfc:64'>
-
  mso-bidi-font-family:Arial;color:#282828;mso-fareast-language:ES-MX'>This</span></span><span
+
 
-
  lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:
+
<span style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Arial;color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>Gene that produces  
-
  "Times New Roman";mso-bidi-font-family:Arial;color:#282828;mso-fareast-language:
+
 
-
  ES-MX'> <span class=SpellE>promoter</span> has a <span class=SpellE>length</span>
+
<span class=SpellE>irrE
-
  of 35 <span class=SpellE>pb</span>(Anderson, 2006).<o:p></o:p></span></p>
+
</span>, a substance that changes the bacteria’s metabolism and allows bacteria to survive to extreme conditions, some examples could be high UV rays exposition, or high salt concentration levels in an aquatic environment, oxidative or thermal shock.
-
  </td>
+
 
-
</tr>
+
<span style='mso-spacerun:yes'>  
-
<tr style='mso-yfti-irow:1;height:75.0pt'>
+
</span>It has a length of 933pb (
-
  <td width=112 nowrap valign=top style='width:84.0pt;border-top:solid #FABF8F 1.0pt;
+
 
-
  mso-border-top-themecolor:accent6;mso-border-top-themetint:153;border-left:
+
<span class=SpellE>Sohrabi
-
  none;border-bottom:none;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor:
+
</span>, 2012).<o:p></o:p>
-
  accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt;
+
</span></p>  
-
  mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt:
+
</td>  
-
  solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint:
+
</tr>  
-
  153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt;
+
<tr style='mso-yfti-irow:3;mso-yfti-lastrow:yes;height:62.25pt'>  
-
  height:75.0pt'>
+
<td width=112 nowrap valign=top style='width:84.0pt;border-top:solid #FABF8F 1.0pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;border-left: none;border-bottom:none;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; height:62.25pt'>  
-
  <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt;
+
 
-
  text-align:right;line-height:normal;mso-yfti-cnfc:4'><!--[if gte vml 1]><v:shape
+
<p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:4'>
-
  id="_x0032__x0020_Imagen" o:spid="_x0000_s1032" type="#_x0000_t75" style='position:absolute;
+
<!--[if gte vml 1]><v:shape id="_x0034__x0020_Imagen" o:spid="_x0000_s1030" type="#_x0000_t75" style='position:absolute; left:0;text-align:left;margin-left:22.6pt;margin-top:5.5pt;width:54.75pt; height:52.5pt;z-index:251657728;visibility:visible;mso-wrap-style:square; mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt; mso-wrap-distance-top:0;mso-wrap-distance-right:9pt; mso-wrap-distance-bottom:0;mso-position-horizontal:absolute; mso-position-horizontal-relative:text;mso-position-vertical:absolute; mso-position-vertical-relative:text;mso-width-percent:0; mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'> <v:imagedata src="TablasAromaYResistencia_archivos/image006.png" o:title=""/> <w:wrap type="square"/> </v:shape><![endif]--><![if !vml]><img width=73 height=70 src="https://static.igem.org/mediawiki/2014hs/5/55/TerminatorResistance.png" align=left hspace=12 v:shapes="_x0034__x0020_Imagen"><![endif]><i>
-
  left:0;text-align:left;margin-left:23.1pt;margin-top:6.95pt;width:54.25pt;
+
 
-
  height:49.45pt;z-index:251651584;visibility:visible;mso-wrap-style:square;
+
<span style='font-family:Oxygen; color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191;mso-ansi-language: EN-US;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p>
-
  mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt;
+
</span></i><i>
-
  mso-wrap-distance-top:0;mso-wrap-distance-right:9pt;
+
 
-
  mso-wrap-distance-bottom:0;mso-position-horizontal:absolute;
+
<span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:"Times New Roman";color:black; mso-fareast-language:ES-MX'> <o:p></o:p>  
-
  mso-position-horizontal-relative:text;mso-position-vertical:absolute;
+
</span></i></p>  
-
  mso-position-vertical-relative:text;mso-width-percent:0;
+
</td>  
-
  mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'>
+
<td width=119 nowrap valign=top style='width:89.0pt;border-top:none; border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; mso-border-right-themecolor:accent6;mso-border-right-themetint:153; mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; padding:0cm 5.4pt 0cm 5.4pt;height:62.25pt'>  
-
  <v:imagedata src="TablasAromaYResistencia_archivos/image003.png" o:title=""/>
+
 
-
  <w:wrap type="square"/>
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal'>
-
  </v:shape><![endif]--><![if !vml]><img width=72 height=66
+
 
-
  src="https://static.igem.org/mediawiki/2014hs/5/5d/Cds.png" align=left hspace=12
+
<span lang=ES-MX style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: "Times New Roman";color:black;mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_B1002">BBa_B1002</a><o:p></o:p>
-
  v:shapes="_x0032__x0020_Imagen"><![endif]><i><span style='font-family:Oxygen;
+
</span></p>  
-
  color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191;mso-ansi-language:
+
 
-
  EN-US;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p></span></i></p>
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal'>&nbsp;</p>
-
  <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt;
+
</td>  
-
  text-align:right;line-height:normal;mso-yfti-cnfc:4'>&nbsp;</p>
+
<td width=400 valign=top style='width:300.0pt;border-top:none;border-left: none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;padding:0cm 5.4pt 0cm 5.4pt;height:62.25pt'>  
-
  </td>
+
 
-
  <td width=119 nowrap valign=top style='width:89.0pt;border-top:none;
+
<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:normal'>
-
  border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:
+
 
-
  accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;
+
<span style='font-size:12.0pt;font-family:Oxygen; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman"; color:black;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>Part made of 6pb responsible for transcription stop (Huang, 206).<o:p></o:p>
-
  mso-border-right-themecolor:accent6;mso-border-right-themetint:153;
+
</span></p>  
-
  mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6;
+
</td>  
-
  mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt;
+
</tr>
-
  mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt:
+
-
  solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153;
+
-
  padding:0cm 5.4pt 0cm 5.4pt;height:75.0pt'>
+
-
  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+
-
  text-align:center;line-height:normal'><span style='font-size:12.0pt;
+
-
  font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:
+
-
  "Times New Roman";color:black;mso-ansi-language:EN-US;mso-fareast-language:
+
-
  ES-MX'><a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a><o:p></o:p></span></p>
+
-
  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+
-
  text-align:center;line-height:normal'>&nbsp;</p>
+
-
  </td>
+
-
  <td width=400 valign=top style='width:300.0pt;border-top:none;border-left:
+
-
  none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;
+
-
  mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor:
+
-
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  solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint:
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  153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6;
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  mso-border-themetint:153;padding:0cm 5.4pt 0cm 5.4pt;height:75.0pt'>
+
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  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align:
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  justify;line-height:normal'><span style='font-size:12.0pt;font-family:Oxygen;
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  color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>This
+
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  specific is <span class=SpellE>RBS</span> based on <span class=SpellE>Elowitz</span>
+
-
  <span class=SpellE>repressilator</span>. It is very common to see in many <span
+
-
  class=SpellE>iGEM</span> projects.<span style='mso-spacerun:yes'>  </span>It
+
-
  has a length of 12 <span class=SpellE>pb </span>(Mahajan, <span
+
-
  class=SpellE>Marinescu</span>, Chow, <span class=SpellE>Wissner</span>-Gross,
+
-
  &amp; Carr, 2003).<o:p></o:p></span></p>
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  <w:wrap type="square"/>
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  </v:shape><![endif]--><![if !vml]><img width=76 height=64
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  mso-bidi-font-family:"Times New Roman";color:black;mso-ansi-language:EN-US;
+
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  mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_K729001">BBa_K729001</a><o:p></o:p></span></p>
+
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  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
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  text-align:center;line-height:normal;mso-yfti-cnfc:64'><span
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  accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt;
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  mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt:
+
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  solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint:
+
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  153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6;
+
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  mso-border-themetint:153;background:#FDE9D9;mso-background-themecolor:accent6;
+
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  mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:90.35pt'>
+
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  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align:
+
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  justify;line-height:normal;mso-yfti-cnfc:64'><span style='font-size:12.0pt;
+
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  font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:
+
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  Arial;color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>Gene
+
-
  that produces <span class=SpellE>irrE</span>, a substance that changes the
+
-
  bacteria’s metabolism and allows bacteria to survive to extreme conditions,
+
-
  some examples could be high UV rays exposition, or high salt concentration
+
-
  levels in an aquatic environment, oxidative or thermal shock.<span
+
-
  style='mso-spacerun:yes'>  </span>It has a length of 933pb (<span
+
-
  class=SpellE>Sohrabi</span>, 2012).<o:p></o:p></span></p>
+
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  </td>
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  solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint:
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+
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+
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+
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  text-align:center;line-height:normal'><span lang=ES-MX style='font-size:12.0pt;
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  font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:
+
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  "Times New Roman";color:black;mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_B1002">BBa_B1002</a><o:p></o:p></span></p>
+
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  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+
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  text-align:center;line-height:normal'>&nbsp;</p></td>
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  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align:
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  color:black;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>Part made of
+
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  6pb responsible for transcription stop (Huang, 206).<o:p></o:p></span></p>
+
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  </td>
+
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</tr>
+
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</div></center>
</div></center>
 +
<p><b><h2>Justifications</h2></b></p>
-
<br><p><b>Bibliography</b></p>
+
<p>The resistance module is very important in our project because it allows the correct function of the bio-filter. Because the objective is to remove the sodium ions from the water, E. CARU, at the beggining of the process, will be surrounded by salt molecules which normally <i>E. coli</i>  could not support.</p>
 +
 
 +
<p>IrrE gives resistance to the high salt concentration of the water, allowing the Nhas gene to function and capture the sodium ions.</p>
 +
 
 +
<p>Another important aspect in the usage of the IrrE gene, is because the promoter in the <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_capture">Capture module</a> is activated with UV rays at 360 wv, intensity that can cause mutations to the bacterium. IrrE protects <i>E. coli </i>  from UV rays and allows the bacteria to their work. </p>
 +
<br>
 +
 
 +
<p><b><h2>Other teams that used IRRE</h2></b></p>
 +
 
 +
<p><b><a href="https://2012.igem.org/Team:University_College_London">UCL 2012</a>:</b> They propose to confer salt tolerance on <i>E. coli</i> by linking the salt tolerance gene encoding the protein irrE (<a href="http://parts.igem.org/Part:BBa_K729001">BBa_K729001</a>) to a constitutive promoter (<a href="http://parts.igem.org/Part:BBa_J23119">BBa_J23119</a>). </p><center>
 +
 
 +
<p><img width=661 height=139 src="https://static.igem.org/mediawiki/2014hs/3/33/Gen1cideb2014.png"align=center hspace=12 alt="IMG_0317"></p>
 +
 
 +
<p><b>Image 3.</b> Part designed by <a href="https://2012.igem.org/Team:University_College_London">UCL 2012</a> for the irrE protein.</p></center>
 +
<br>
 +
 
 +
 
 +
<p><h2><b>Project Zoom In</b></h2></p></br><center><iframe width="600" height="500" src="//www.youtube.com/embed/qAYmPrq9NsY" frameborder="0" allowfullscreen></iframe></center>
 +
<br>
 +
 
 +
<p><b><h2>Bibliography/References</h2></b></p><font size="2">
-
<font size="2">
 
<p>● Antiquity 2013. (2013, January 31). <i>Part:BBa_B0034</i>. Retrieved from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034">http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034</a>.</p>
<p>● Antiquity 2013. (2013, January 31). <i>Part:BBa_B0034</i>. Retrieved from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034">http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034</a>.</p>
 +
<p>● Huang, H. (2006, August 30). <i>Part:BBa_B1002</i>. Retrieved August 30, 2014, from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B1002">http://parts.igem.org/wiki/index.php?title=Part:BBa_B1002</a>.</p>
<p>● Huang, H. (2006, August 30). <i>Part:BBa_B1002</i>. Retrieved August 30, 2014, from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B1002">http://parts.igem.org/wiki/index.php?title=Part:BBa_B1002</a>.</p>
 +
<p>● iGEM2006_Berkeley. (2006, August 24). <i>Part:BBa_J23119</i>. Retrieved April 30, 2014, from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_J23119">http://parts.igem.org/wiki/index.php?title=Part:BBa_J23119</a>.</p>
<p>● iGEM2006_Berkeley. (2006, August 24). <i>Part:BBa_J23119</i>. Retrieved April 30, 2014, from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_J23119">http://parts.igem.org/wiki/index.php?title=Part:BBa_J23119</a>.</p>
 +
<p>● Sohrabi, B. (2012, June 27). <i>Part:BBa_K729001</i>. Retrieved from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K729001">http://parts.igem.org/wiki/index.php?title=Part:BBa_K729001</a>.</p>
<p>● Sohrabi, B. (2012, June 27). <i>Part:BBa_K729001</i>. Retrieved from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K729001">http://parts.igem.org/wiki/index.php?title=Part:BBa_K729001</a>.</p>
-
<p>● UCLiGEM Team. (2012). <i>IRRE module</i>. Retrieved March 31, 2014. from <a href="https://2012.igem.org/Team:University_College_London/Module_5">https://2012.igem.org/Team:University_College_London/Module_5</a>.</p>
 
-
</font>
 
 +
<p>● UCLiGEM Team. (2012). <i>IRRE module</i>. Retrieved March 31, 2014. from <a href="https://2012.igem.org/Team:University_College_London/Module_5">https://2012.igem.org/Team:University_College_London/Module_5</a>.</p></font>
<br>
<br>
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<div style="text-align: right;"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_resistance#"><font color="blue">Return to the Top</font></a></p></div>
 
 +
<div style="text-align: right;"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_resistance#"><font color="blue">Return to the Top</font></a></p>
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Latest revision as of 15:26, 19 June 2014

Description Capture Module Aroma Module Resistance Module Union Module Parts
iGEM CIDEB 2014 - Project

Resistance Module

Because our project is a biofilter which allows to remove salt from water, the bacteria needs to survive to extreme environmental conditions which normally it can’t do. We need E. coli to survive a high salinity environment to allow it to capture sodium ions and to remove the salt concentration of the water.

In order to accomplish our project goal, we have to change E. coli metabolism and make it stronger, more resistant and more efficient than in normal E. coli bacteria.

Last discoveries show irrE as a protein capable of change the E. coli metabolism, and giving it the ability to survive to bigger temperatures, bigger UV rays radiation and bigger salt concentration (UCL, 2012).


Description

The protein irrE originates from Deinococcus radiodurans, and initially, this gene provides resistance to radiation. But when transformed in E. coli, it protects it against salt, oxidative and thermal shock. (UCL, 2012) Also, different experiments from different iGEM teams, support the idea of the bigger salt resistance in E. coli with this biobrick.

IMG_0317

Image 1. This graph done by UCL iGEM 2012, shows how irrE biobrick increased the salt concentration resistance in E. coli compared with the results from Tu Delf iGEM 2010.


irrE has been demonstrated to up regulate transcription of recA and pprA - genes which encode Recombines A and Radiation Inducible Protein. With respect to salt tolerance, irrE up regulates the production of several stress responsive proteins, protein kinases, metabolic proteins, and detoxification proteins. It also down regulates glycerol degradation. With this global regulatory effect, E. coli becomes more salt tolerant (UCL, 2012).

IMG_0317

Image 2. This diagram shows the effect of irrE protein on E. coli metabolism.

Device

Initially, IrrE and L2+AIDA, protein for binding silica (Union module), were joined together in only one circuit, but we needed to separate them because L2+AIDA has not been proved yet and it could affect the correct production of IrrE (see image 3).

IMG_0317

Image 3. Circuit for our project and for testing resistance and union modules.


Parts of the Module


IMAGE

CODE

DESCRIPTION

 

BBa_J23119

 

In the specific case of our bacteria, it helps to continuously transcribing the irrE gene in order to make bacteria resist high concentration of salt. This promoter has a length of 35 pb (Anderson, 2006).

 

BBa_B0034

 

This specific is RBS based on Elowitz repressilator . It is very common to see in many iGEM projects. It has a length of 12 pb (Mahajan, Marinescu , Chow, Wissner -Gross, & Carr, 2003).

BBa_K729001

Gene that produces irrE , a substance that changes the bacteria’s metabolism and allows bacteria to survive to extreme conditions, some examples could be high UV rays exposition, or high salt concentration levels in an aquatic environment, oxidative or thermal shock. It has a length of 933pb ( Sohrabi , 2012).

BBa_B1002

 

Part made of 6pb responsible for transcription stop (Huang, 206).

Justifications

The resistance module is very important in our project because it allows the correct function of the bio-filter. Because the objective is to remove the sodium ions from the water, E. CARU, at the beggining of the process, will be surrounded by salt molecules which normally E. coli could not support.

IrrE gives resistance to the high salt concentration of the water, allowing the Nhas gene to function and capture the sodium ions.

Another important aspect in the usage of the IrrE gene, is because the promoter in the Capture module is activated with UV rays at 360 wv, intensity that can cause mutations to the bacterium. IrrE protects E. coli from UV rays and allows the bacteria to their work.


Other teams that used IRRE

UCL 2012: They propose to confer salt tolerance on E. coli by linking the salt tolerance gene encoding the protein irrE (BBa_K729001) to a constitutive promoter (BBa_J23119).

IMG_0317

Image 3. Part designed by UCL 2012 for the irrE protein.


Project Zoom In



Bibliography/References

● Antiquity 2013. (2013, January 31). Part:BBa_B0034. Retrieved from http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034.

● Huang, H. (2006, August 30). Part:BBa_B1002. Retrieved August 30, 2014, from http://parts.igem.org/wiki/index.php?title=Part:BBa_B1002.

● iGEM2006_Berkeley. (2006, August 24). Part:BBa_J23119. Retrieved April 30, 2014, from http://parts.igem.org/wiki/index.php?title=Part:BBa_J23119.

● Sohrabi, B. (2012, June 27). Part:BBa_K729001. Retrieved from http://parts.igem.org/wiki/index.php?title=Part:BBa_K729001.

● UCLiGEM Team. (2012). IRRE module. Retrieved March 31, 2014. from https://2012.igem.org/Team:University_College_London/Module_5.
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