Team:CIDEB-UANL Mexico/project resistance

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<p>Because our project is a biofilter which allows to remove salt from water, the bacteria needs to survive to extreme environmental conditions which normally it can’t do. We need <i>E. coli</i>  to survive a high salinity environment to allow it to capture sodium ions and to remove the salt concentration of the water.</p>
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<p>In order to accomplish our project goal, we have to change <i>E. coli</i> metabolism and make it stronger, more resistant and more efficient than in normal <i>E. coli</i> bacteria.</p>
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<p>Last discoveries show irrE as a protein capable of change the <i>E. coli</i> metabolism, and giving it the ability to survive to bigger temperatures, bigger UV rays radiation and bigger salt concentration (UCL, 2012).</p>
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<p><b><h2>Description</h2></b></p>  
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<p>Because our project is a biofilter which allows to remove salt from water, the bacteria needs to survive to extreme environmental conditions which normally it can’t do. We need <i>E. Coli</i> to survive a high salinity environment to allow it to capture Na ions and remove the salt concentration of the water.</p>
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<p>The protein irrE originates from <i>Deinococcus radiodurans</i>, and initially, this gene provides resistance to radiation. But when transformed in <i>E. coli</i>, it protects it against salt, oxidative and thermal shock. (UCL, 2012) Also, different experiments from different iGEM teams, support the idea of the bigger salt resistance in <i>E. coli</i> with this biobrick. </p><center>
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<p>In order to accomplish our project goal, we have to change <i>E. Coli</i> metabolism and make it stronger, more resistant and more efficient than in normal E.coli bacteria.</p>
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<p><img width=564 height=350 src="https://static.igem.org/mediawiki/2014hs/3/3e/Grafica_1_cideb2014.png"align=center hspace=12 alt="IMG_0317"></p>
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<p>Last discoveries show IrrE as a protein capable of change the <i>E. Coli</i> metabolism, and giving it the ability to survive to bigger temperatures, bigger UV rays radiation and bigger salt concentration <b>(UCL, 2012)</b>.</p>
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<p><b>Image 1.</b> This graph done by <a herf="https://2012.igem.org/Team:University_College_London">UCL iGEM 2012</a>, shows how irrE biobrick increased the salt concentration resistance in <i>E. coli</i> compared with the results from <a href="https://2010.igem.org/Team:TU_Delft">Tu Delf iGEM 2010</a>.</p></center>
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<br><p><b>¿What does IrrE do?</b></p>
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<p>irrE has been demonstrated to up regulate transcription of recA and pprA - genes which encode Recombines A and Radiation Inducible Protein. With respect to salt tolerance, irrE up regulates the production of several stress responsive proteins, protein kinases, metabolic proteins, and detoxification proteins. It also down regulates glycerol degradation. With this global regulatory effect, <i>E. coli</i> becomes more salt tolerant (UCL, 2012).</p><center>
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<p>The protein IrrE originates from <i>Deinococcusradiodurans</i>, and initially, this gene provides resistance to radiation. But when transformed in <i>E. Coli</i>, it protects it against salt, oxidative and thermal shock. <b>(UCL, 2012)</b> Also, different experiments from different iGEM teams, support the idea of the bigger salt resistance in <i>E. Coli</i> with this biobrick. </p>
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<p><img width=429 height=322 src="https://static.igem.org/mediawiki/2014hs/9/9b/Imagen1cideb2014.png"align=center hspace=12 alt="IMG_0317"></p>
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<center><p><img width=564 height=350 src="https://static.igem.org/mediawiki/2014hs/3/3e/Grafica_1_cideb2014.png"
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<p><b>Image 2.</b> This diagram shows the effect of irrE protein on <i>E. coli</i> metabolism. </p></center>
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<p><b><h2>Device</h2></b></p>
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<p><b>Image 1.</b> This graph done by <b>UCL iGEM team 2012</b>, shows how IrrE biobrick increased the salt concentration resistance in <i>E. Coli</i> compared with the results from <b>Tu Delf iGEM team 2010</b>.</p></center>
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<p>Initially, IrrE and L2+AIDA, protein for binding silica (<a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_union">Union module</a>), were joined together in only one circuit, but we needed to separate them because L2+AIDA has not been proved yet and it could affect the correct production of IrrE (see image 3).</p><center>
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<br><p><b>How it works?</b></p>
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<p><img width=529 height=322 src="https://static.igem.org/mediawiki/2014hs/0/05/Circuit_l2_and_irre.png"align=center hspace=12 alt="IMG_0317"></p>
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<p>IrrE has been demonstrated to up regulate transcription of recA and pprA - genes which encode Recombines A and Radiation Inducible Protein. With respect to salt tolerance, IrrE up regulates the production of several stress responsive proteins, protein kinases, metabolic proteins, and detoxification proteins. It also down regulates glycerol degradation. With this global regulatory effect, <i>E. Coli</i> becomes more salt tolerant <b>(UCL, 2012)</b>.</p>
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<p><b>Image 3.</b> Circuit for our project and for testing resistance and union modules. </p></center>
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<p><b><h2>Parts of the Module</h2></b></p>
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<br><center>
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<p><b>Image 2.</b> This diagram shows the effect of IrrE protein on <i>E. Coli</i> metabolism. </p></center>
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<br><p><b>Other teams that use it:</b></p>
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<p><b>London 2012:</b> They propose to confer salt tolerance on <i>E. Coli</i> by linking the salt tolerance gene encoding the protein IrrE (BBa_K729001) to a constitutive promoter (BBa_J23119). </p>
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<td width=119 nowrap valign=top style='width:89.0pt;border:none;border-bottom: solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint: 153;mso-border-bottom-alt:solid #FABF8F .5pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor: background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'>  
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<p><b>Image 3.</b> Part designed by <b>UCL iGEM team 2012</b> for the irrE protein.</p></center>
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:1'><b>
-
<br><p><b>IrrE's parts description :</b></p><br>
+
<span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX'>CODE
 +
</span></b><b style='mso-bidi-font-weight:normal'>
-
<center><div>
+
<span lang=ES-MX style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: "Times New Roman";mso-fareast-language:ES-MX'><o:p></o:p>
-
<table class=MsoTable15Grid7ColorfulAccent6 border=1 cellspacing=0
+
</span></b></p>  
-
cellpadding=0 width=631 style='width:473.0pt;border-collapse:collapse;
+
</td>  
-
border:none;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6;
+
<td width=400 nowrap valign=top style='width:300.0pt;border:none;border-bottom: solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint: 153;mso-border-bottom-alt:solid #FABF8F .5pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor: background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'>  
-
mso-border-themetint:153;mso-yfti-tbllook:1184;mso-padding-alt:0cm 5.4pt 0cm 5.4pt'>
+
 
-
<tr style='mso-yfti-irow:-1;mso-yfti-firstrow:yes;mso-yfti-lastfirstrow:yes;
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:1'><b>
-
  height:15.75pt'>
+
 
-
  <td width=112 nowrap valign=top style='width:84.0pt;border:none;border-bottom:
+
<span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX'>DESCRIPTION
-
  solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint:
+
</span></b><b style='mso-bidi-font-weight:normal'>
-
  153;mso-border-bottom-alt:solid #FABF8F .5pt;mso-border-bottom-themecolor:
+
 
-
  accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor:
+
<span lang=ES-MX style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: "Times New Roman";mso-fareast-language:ES-MX'><o:p></o:p>
-
  background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'>
+
</span></b></p>  
-
  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+
</td>  
-
  text-align:center;line-height:normal;mso-yfti-cnfc:517'><b><span lang=ES-MX
+
</tr>  
-
  style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman";
+
<tr style='mso-yfti-irow:0;height:60.75pt'>  
-
  mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX;mso-bidi-font-style:
+
<td width=112 nowrap valign=top style='width:84.0pt;border:none;border-right: solid #FABF8F 1.0pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; height:60.75pt'>  
-
  italic'>IMAGE</span></b><b style='mso-bidi-font-weight:normal'><span
+
 
-
  lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:
+
<p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:68'>
-
  "Times New Roman";mso-bidi-font-family:"Times New Roman";mso-fareast-language:
+
<!--[if gte vml 1]><v:shapetype id="_x0000_t75" coordsize="21600,21600" o:spt="75" o:preferrelative="t" path="m@4@5l@4@11@9@11@9@5xe" filled="f" stroked="f"> <v:stroke joinstyle="miter"/> <v:formulas> <v:f eqn="if lineDrawn pixelLineWidth 0"/> <v:f eqn="sum @0 1 0"/> <v:f eqn="sum 0 0 @1"/> <v:f eqn="prod @2 1 2"/> <v:f eqn="prod @3 21600 pixelWidth"/> <v:f eqn="prod @3 21600 pixelHeight"/> <v:f eqn="sum @0 0 1"/> <v:f eqn="prod @6 1 2"/> <v:f eqn="prod @7 21600 pixelWidth"/> <v:f eqn="sum @8 21600 0"/> <v:f eqn="prod @7 21600 pixelHeight"/> <v:f eqn="sum @10 21600 0"/> </v:formulas> <v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/> <o:lock v:ext="edit" aspectratio="t"/> </v:shapetype><v:shape id="_x0031__x0020_Imagen" o:spid="_x0000_s1033" type="#_x0000_t75" style='position:absolute;left:0;text-align:left; margin-left:15.9pt;margin-top:9.9pt;width:61.45pt;height:48.6pt;z-index:251649536; visibility:visible;mso-wrap-style:square;mso-width-percent:0; mso-height-percent:0;mso-wrap-distance-left:9pt;mso-wrap-distance-top:0; mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:0; mso-position-horizontal:absolute;mso-position-horizontal-relative:text; mso-position-vertical:absolute;mso-position-vertical-relative:text; mso-width-percent:0;mso-height-percent:0;mso-width-relative:page; mso-height-relative:page'> <v:imagedata src="TablasAromaYResistencia_archivos/image001.png" o:title=""/> <w:wrap type="square"/> </v:shape><![endif]--><![if !vml]><img width=82 height=65 src="https://static.igem.org/mediawiki/2014hs/6/62/Promotor.png" align=left hspace=12 v:shapes="_x0031__x0020_Imagen"><![endif]><i>
-
  ES-MX;mso-bidi-font-style:italic'><o:p></o:p></span></b></p>
+
 
-
  </td>
+
<span lang=ES-MX style='font-family:Oxygen;color:#E36C0A;mso-themecolor:accent6;mso-themeshade: 191;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p>
-
  <td width=119 nowrap valign=top style='width:89.0pt;border:none;border-bottom:
+
</span></i></p>  
-
  solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint:
+
 
-
  153;mso-border-bottom-alt:solid #FABF8F .5pt;mso-border-bottom-themecolor:
+
<p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:68'>&nbsp;</p>
-
  accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor:
+
</td>  
-
  background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'>
+
<td width=119 nowrap valign=top style='width:89.0pt;border-top:none; border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; mso-border-right-themecolor:accent6;mso-border-right-themetint:153; mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; background:#FDE9D9;mso-background-themecolor:accent6;mso-background-themetint: 51;padding:0cm 5.4pt 0cm 5.4pt;height:60.75pt'>  
-
  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+
 
-
  text-align:center;line-height:normal;mso-yfti-cnfc:1'><b><span lang=ES-MX
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:64'>
-
  style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman";
+
 
-
  mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX'>CODE</span></b><b
+
<span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";color:black;mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_J23119">BBa_J23119</a><o:p></o:p>
-
  style='mso-bidi-font-weight:normal'><span lang=ES-MX style='font-size:12.0pt;
+
</span></p>  
-
  font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:
+
 
-
  "Times New Roman";mso-fareast-language:ES-MX'><o:p></o:p></span></b></p>
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:64'>&nbsp;</p>  
-
  </td>
+
</td>  
-
  <td width=400 nowrap valign=top style='width:300.0pt;border:none;border-bottom:
+
<td width=400 valign=top style='width:300.0pt;border-top:none;border-left: none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;background:#FDE9D9;mso-background-themecolor:accent6; mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:60.75pt'>  
-
  solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;mso-border-bottom-themetint:
+
 
-
  153;mso-border-bottom-alt:solid #FABF8F .5pt;mso-border-bottom-themecolor:
+
<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:normal;mso-yfti-cnfc:64'>
-
  accent6;mso-border-bottom-themetint:153;background:white;mso-background-themecolor:
+
 
-
  background1;padding:0cm 5.4pt 0cm 5.4pt;height:15.75pt'>
+
<span style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Arial;color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>In the specific case of our bacteria, it helps to continuously transcribing the  
-
  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+
 
-
  text-align:center;line-height:normal;mso-yfti-cnfc:1'><b><span lang=ES-MX
+
<span class=SpellE>irrE
-
  style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman";
+
</span> gene in order to make bacteria resist high concentration of salt.  
-
  mso-bidi-font-family:"Times New Roman";mso-fareast-language:ES-MX'>DESCRIPTION</span></b><b
+
</span>
-
  style='mso-bidi-font-weight:normal'><span lang=ES-MX style='font-size:12.0pt;
+
 
-
  font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:
+
<span class=SpellE>
-
  "Times New Roman";mso-fareast-language:ES-MX'><o:p></o:p></span></b></p>
+
 
-
  </td>
+
<span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:Arial;color:#282828;mso-fareast-language:ES-MX'>This
-
</tr>
+
</span>
-
<tr style='mso-yfti-irow:0;height:60.75pt'>
+
</span>
-
  <td width=112 nowrap valign=top style='width:84.0pt;border:none;border-right:
+
 
-
  solid #FABF8F 1.0pt;mso-border-right-themecolor:accent6;mso-border-right-themetint:
+
<span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Arial;color:#282828;mso-fareast-language: ES-MX'>  
-
  153;mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6;
+
 
-
  mso-border-top-themetint:153;mso-border-top-alt:solid #FABF8F .5pt;
+
<span class=SpellE>promoter
-
  mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt:
+
</span> has a  
-
  solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint:
+
 
-
  153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt;
+
<span class=SpellE>length
-
  height:60.75pt'>
+
</span> of 35  
-
  <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt;
+
 
-
  text-align:right;line-height:normal;mso-yfti-cnfc:68'><!--[if gte vml 1]><v:shapetype
+
<span class=SpellE>pb
-
  id="_x0000_t75" coordsize="21600,21600" o:spt="75" o:preferrelative="t"
+
</span>(Anderson, 2006).<o:p></o:p>
-
  path="m@4@5l@4@11@9@11@9@5xe" filled="f" stroked="f">
+
</span></p>  
-
  <v:stroke joinstyle="miter"/>
+
</td>  
-
  <v:formulas>
+
</tr>  
-
    <v:f eqn="if lineDrawn pixelLineWidth 0"/>
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<tr style='mso-yfti-irow:1;height:75.0pt'>  
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    <v:f eqn="sum @0 1 0"/>
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<td width=112 nowrap valign=top style='width:84.0pt;border-top:solid #FABF8F 1.0pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;border-left: none;border-bottom:none;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; height:75.0pt'>  
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    <v:f eqn="sum 0 0 @1"/>
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-
    <v:f eqn="prod @2 1 2"/>
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<p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:4'>
-
    <v:f eqn="prod @3 21600 pixelWidth"/>
+
<!--[if gte vml 1]><v:shape id="_x0032__x0020_Imagen" o:spid="_x0000_s1032" type="#_x0000_t75" style='position:absolute; left:0;text-align:left;margin-left:23.1pt;margin-top:6.95pt;width:54.25pt; height:49.45pt;z-index:251651584;visibility:visible;mso-wrap-style:square; mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt; mso-wrap-distance-top:0;mso-wrap-distance-right:9pt; mso-wrap-distance-bottom:0;mso-position-horizontal:absolute; mso-position-horizontal-relative:text;mso-position-vertical:absolute; mso-position-vertical-relative:text;mso-width-percent:0; mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'> <v:imagedata src="TablasAromaYResistencia_archivos/image003.png" o:title=""/> <w:wrap type="square"/> </v:shape><![endif]--><![if !vml]><img width=72 height=66 src="https://static.igem.org/mediawiki/2014hs/5/5d/Cds.png" align=left hspace=12 v:shapes="_x0032__x0020_Imagen"><![endif]><i>
-
    <v:f eqn="prod @3 21600 pixelHeight"/>
+
 
-
    <v:f eqn="sum @0 0 1"/>
+
<span style='font-family:Oxygen; color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191;mso-ansi-language: EN-US;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p>
-
    <v:f eqn="prod @6 1 2"/>
+
</span></i></p>  
-
    <v:f eqn="prod @7 21600 pixelWidth"/>
+
 
-
    <v:f eqn="sum @8 21600 0"/>
+
<p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:4'>&nbsp;</p>  
-
    <v:f eqn="prod @7 21600 pixelHeight"/>
+
</td>  
-
    <v:f eqn="sum @10 21600 0"/>
+
<td width=119 nowrap valign=top style='width:89.0pt;border-top:none; border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; mso-border-right-themecolor:accent6;mso-border-right-themetint:153; mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; padding:0cm 5.4pt 0cm 5.4pt;height:75.0pt'>  
-
  </v:formulas>
+
 
-
  <v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/>
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal'>
-
  <o:lock v:ext="edit" aspectratio="t"/>
+
 
-
  </v:shapetype><v:shape id="_x0031__x0020_Imagen" o:spid="_x0000_s1033"
+
<span style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: "Times New Roman";color:black;mso-ansi-language:EN-US;mso-fareast-language: ES-MX'><a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a><o:p></o:p>
-
  type="#_x0000_t75" style='position:absolute;left:0;text-align:left;
+
</span></p>  
-
  margin-left:15.9pt;margin-top:9.9pt;width:61.45pt;height:48.6pt;z-index:251649536;
+
 
-
  visibility:visible;mso-wrap-style:square;mso-width-percent:0;
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal'>&nbsp;</p>  
-
  mso-height-percent:0;mso-wrap-distance-left:9pt;mso-wrap-distance-top:0;
+
</td>  
-
  mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:0;
+
<td width=400 valign=top style='width:300.0pt;border-top:none;border-left: none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;padding:0cm 5.4pt 0cm 5.4pt;height:75.0pt'>  
-
  mso-position-horizontal:absolute;mso-position-horizontal-relative:text;
+
 
-
  mso-position-vertical:absolute;mso-position-vertical-relative:text;
+
<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:normal'>
-
  mso-width-percent:0;mso-height-percent:0;mso-width-relative:page;
+
 
-
  mso-height-relative:page'>
+
<span style='font-size:12.0pt;font-family:Oxygen; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Arial; color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>This specific is  
-
  <v:imagedata src="TablasAromaYResistencia_archivos/image001.png" o:title=""/>
+
 
-
  <w:wrap type="square"/>
+
<span class=SpellE>RBS
-
  </v:shape><![endif]--><![if !vml]><img width=82 height=65
+
</span> based on  
-
  src="https://static.igem.org/mediawiki/2014hs/6/62/Promotor.png" align=left hspace=12
+
 
-
  v:shapes="_x0031__x0020_Imagen"><![endif]><i><span lang=ES-MX
+
<span class=SpellE>Elowitz
-
  style='font-family:Oxygen;color:#E36C0A;mso-themecolor:accent6;mso-themeshade:
+
</span>  
-
  191;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p></span></i></p>
+
 
-
  <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt;
+
<span class=SpellE>repressilator
-
  text-align:right;line-height:normal;mso-yfti-cnfc:68'>&nbsp;</p></td>
+
</span>. It is very common to see in many  
-
  <td width=119 nowrap valign=top style='width:89.0pt;border-top:none;
+
 
-
  border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:
+
<span class=SpellE>iGEM
-
  accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;
+
</span> projects.
-
  mso-border-right-themecolor:accent6;mso-border-right-themetint:153;
+
 
-
  mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6;
+
<span style='mso-spacerun:yes'>  
-
  mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt;
+
</span>It has a length of 12  
-
  mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt:
+
 
-
  solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153;
+
<span class=SpellE>pb  
-
  background:#FDE9D9;mso-background-themecolor:accent6;mso-background-themetint:
+
</span>(Mahajan,  
-
  51;padding:0cm 5.4pt 0cm 5.4pt;height:60.75pt'>
+
 
-
  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+
<span class=SpellE>Marinescu
-
  text-align:center;line-height:normal;mso-yfti-cnfc:64'><span lang=ES-MX
+
</span>, Chow,  
-
  style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman";
+
 
-
  mso-bidi-font-family:"Times New Roman";color:black;mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_J23119">BBa_J23119</a><o:p></o:p></span></p>
+
<span class=SpellE>Wissner
-
  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+
</span>-Gross, &amp; Carr, 2003).<o:p></o:p>
-
  text-align:center;line-height:normal;mso-yfti-cnfc:64'>&nbsp;</p>
+
</span></p>  
-
  </td>
+
</td>  
-
  <td width=400 valign=top style='width:300.0pt;border-top:none;border-left:
+
</tr>  
-
  none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;
+
<tr style='mso-yfti-irow:2;height:90.35pt'>  
-
  mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor:
+
<td width=112 nowrap valign=top style='width:84.0pt;border-top:solid #FABF8F 1.0pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;border-left: none;border-bottom:none;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; height:90.35pt'>  
-
  accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt;
+
 
-
  mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt:
+
<p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:68'>
-
  solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint:
+
<!--[if gte vml 1]><v:shape id="_x0033__x0020_Imagen" o:spid="_x0000_s1031" type="#_x0000_t75" style='position:absolute; left:0;text-align:left;margin-left:23.4pt;margin-top:10pt;width:57pt; height:48pt;z-index:251654656;visibility:visible;mso-wrap-style:square; mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt; mso-wrap-distance-top:0;mso-wrap-distance-right:9pt; mso-wrap-distance-bottom:0;mso-position-horizontal:absolute; mso-position-horizontal-relative:text;mso-position-vertical:absolute; mso-position-vertical-relative:text;mso-width-percent:0; mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'> <v:imagedata src="TablasAromaYResistencia_archivos/image005.png" o:title=""/> <w:wrap type="square"/> </v:shape><![endif]--><![if !vml]><img width=76 height=64 src="https://static.igem.org/mediawiki/2014hs/9/97/IrrEResistance.png" align=left hspace=12 v:shapes="_x0033__x0020_Imagen"><![endif]></p>  
-
  153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6;
+
</td>  
-
  mso-border-themetint:153;background:#FDE9D9;mso-background-themecolor:accent6;
+
<td width=119 nowrap valign=top style='width:89.0pt;border-top:none; border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; mso-border-right-themecolor:accent6;mso-border-right-themetint:153; mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; background:#FDE9D9;mso-background-themecolor:accent6;mso-background-themetint: 51;padding:0cm 5.4pt 0cm 5.4pt;height:90.35pt'>  
-
  mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:60.75pt'>
+
 
-
  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align:
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:64'>
-
  justify;line-height:normal;mso-yfti-cnfc:64'><span style='font-size:12.0pt;
+
 
-
  font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:
+
<span style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";color:black;mso-ansi-language:EN-US; mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_K729001">BBa_K729001</a><o:p></o:p>
-
  Arial;color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>In
+
</span></p>  
-
  the specific case of our bacteria, it helps to continuously transcribing the <span
+
 
-
  class=SpellE>IrrE</span> gene in order to make bacteria resist high
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal;mso-yfti-cnfc:64'>
-
  concentration of salt. </span><span class=SpellE><span lang=ES-MX
+
 
-
  style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman";
+
<span style='font-size:3.0pt;font-family:Oxygen;mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";color:black;mso-ansi-language:EN-US; mso-fareast-language:ES-MX'><o:p></o:p>
-
  mso-bidi-font-family:Arial;color:#282828;mso-fareast-language:ES-MX'>This</span></span><span
+
</span></p>  
-
  lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family:
+
</td>  
-
  "Times New Roman";mso-bidi-font-family:Arial;color:#282828;mso-fareast-language:
+
<td width=400 valign=top style='width:300.0pt;border-top:none;border-left: none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;background:#FDE9D9;mso-background-themecolor:accent6; mso-background-themetint:51;padding:0cm 5.4pt 0cm 5.4pt;height:90.35pt'>  
-
  ES-MX'> <span class=SpellE>promoter</span> has a <span class=SpellE>length</span>
+
 
-
  of 35 <span class=SpellE>pb</span> <b>(Anderson, 2006)</b>.<o:p></o:p></span></p>
+
<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:normal;mso-yfti-cnfc:64'>
-
  </td>
+
 
-
</tr>
+
<span style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Arial;color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>Gene that produces  
-
<tr style='mso-yfti-irow:1;height:75.0pt'>
+
 
-
  <td width=112 nowrap valign=top style='width:84.0pt;border-top:solid #FABF8F 1.0pt;
+
<span class=SpellE>irrE
-
  mso-border-top-themecolor:accent6;mso-border-top-themetint:153;border-left:
+
</span>, a substance that changes the bacteria’s metabolism and allows bacteria to survive to extreme conditions, some examples could be high UV rays exposition, or high salt concentration levels in an aquatic environment, oxidative or thermal shock.
-
  none;border-bottom:none;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor:
+
 
-
  accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt;
+
<span style='mso-spacerun:yes'>  
-
  mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt:
+
</span>It has a length of 933pb (
-
  solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint:
+
 
-
  153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt;
+
<span class=SpellE>Sohrabi
-
  height:75.0pt'>
+
</span>, 2012).<o:p></o:p>
-
  <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt;
+
</span></p>  
-
  text-align:right;line-height:normal;mso-yfti-cnfc:4'><!--[if gte vml 1]><v:shape
+
</td>  
-
  id="_x0032__x0020_Imagen" o:spid="_x0000_s1032" type="#_x0000_t75" style='position:absolute;
+
</tr>  
-
  left:0;text-align:left;margin-left:23.1pt;margin-top:6.95pt;width:54.25pt;
+
<tr style='mso-yfti-irow:3;mso-yfti-lastrow:yes;height:62.25pt'>  
-
  height:49.45pt;z-index:251651584;visibility:visible;mso-wrap-style:square;
+
<td width=112 nowrap valign=top style='width:84.0pt;border-top:solid #FABF8F 1.0pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;border-left: none;border-bottom:none;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-right-alt: solid #FABF8F .5pt;mso-border-right-themecolor:accent6;mso-border-right-themetint: 153;background:white;mso-background-themecolor:background1;padding:0cm 5.4pt 0cm 5.4pt; height:62.25pt'>  
-
  mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt;
+
 
-
  mso-wrap-distance-top:0;mso-wrap-distance-right:9pt;
+
<p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:right;line-height:normal;mso-yfti-cnfc:4'>
-
  mso-wrap-distance-bottom:0;mso-position-horizontal:absolute;
+
<!--[if gte vml 1]><v:shape id="_x0034__x0020_Imagen" o:spid="_x0000_s1030" type="#_x0000_t75" style='position:absolute; left:0;text-align:left;margin-left:22.6pt;margin-top:5.5pt;width:54.75pt; height:52.5pt;z-index:251657728;visibility:visible;mso-wrap-style:square; mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt; mso-wrap-distance-top:0;mso-wrap-distance-right:9pt; mso-wrap-distance-bottom:0;mso-position-horizontal:absolute; mso-position-horizontal-relative:text;mso-position-vertical:absolute; mso-position-vertical-relative:text;mso-width-percent:0; mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'> <v:imagedata src="TablasAromaYResistencia_archivos/image006.png" o:title=""/> <w:wrap type="square"/> </v:shape><![endif]--><![if !vml]><img width=73 height=70 src="https://static.igem.org/mediawiki/2014hs/5/55/TerminatorResistance.png" align=left hspace=12 v:shapes="_x0034__x0020_Imagen"><![endif]><i>
-
  mso-position-horizontal-relative:text;mso-position-vertical:absolute;
+
 
-
  mso-position-vertical-relative:text;mso-width-percent:0;
+
<span style='font-family:Oxygen; color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191;mso-ansi-language: EN-US;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p>
-
  mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'>
+
</span></i><i>
-
  <v:imagedata src="TablasAromaYResistencia_archivos/image003.png" o:title=""/>
+
 
-
  <w:wrap type="square"/>
+
<span lang=ES-MX style='font-size:12.0pt;font-family:Oxygen;mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:"Times New Roman";color:black; mso-fareast-language:ES-MX'> <o:p></o:p>  
-
  </v:shape><![endif]--><![if !vml]><img width=72 height=66
+
</span></i></p>  
-
  src="https://static.igem.org/mediawiki/2014hs/5/5d/Cds.png" align=left hspace=12
+
</td>  
-
  v:shapes="_x0032__x0020_Imagen"><![endif]><i><span style='font-family:Oxygen;
+
<td width=119 nowrap valign=top style='width:89.0pt;border-top:none; border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor: accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt; mso-border-right-themecolor:accent6;mso-border-right-themetint:153; mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6; mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt; mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt: solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153; padding:0cm 5.4pt 0cm 5.4pt;height:62.25pt'>  
-
  color:#E36C0A;mso-themecolor:accent6;mso-themeshade:191;mso-ansi-language:
+
 
-
  EN-US;mso-fareast-language:ES-MX;mso-no-proof:yes'><o:p></o:p></span></i></p>
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal'>
-
  <p class=MsoNormal align=right style='margin-bottom:0cm;margin-bottom:.0001pt;
+
 
-
  text-align:right;line-height:normal;mso-yfti-cnfc:4'>&nbsp;</p>
+
<span lang=ES-MX style='font-size:12.0pt; font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: "Times New Roman";color:black;mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_B1002">BBa_B1002</a><o:p></o:p>
-
  </td>
+
</span></p>  
-
  <td width=119 nowrap valign=top style='width:89.0pt;border-top:none;
+
 
-
  border-left:none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:
+
<p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt; text-align:center;line-height:normal'>&nbsp;</p>
-
  accent6;mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;
+
</td>  
-
  mso-border-right-themecolor:accent6;mso-border-right-themetint:153;
+
<td width=400 valign=top style='width:300.0pt;border-top:none;border-left: none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6; mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor: accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt; mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt: solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint: 153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6; mso-border-themetint:153;padding:0cm 5.4pt 0cm 5.4pt;height:62.25pt'>  
-
  mso-border-top-alt:solid #FABF8F .5pt;mso-border-top-themecolor:accent6;
+
 
-
  mso-border-top-themetint:153;mso-border-left-alt:solid #FABF8F .5pt;
+
<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align: justify;line-height:normal'>
-
  mso-border-left-themecolor:accent6;mso-border-left-themetint:153;mso-border-alt:
+
 
-
  solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153;
+
<span style='font-size:12.0pt;font-family:Oxygen; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman"; color:black;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>Part made of 6pb responsible for transcription stop (Huang, 206).<o:p></o:p>
-
  padding:0cm 5.4pt 0cm 5.4pt;height:75.0pt'>
+
</span></p>  
-
  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+
</td>  
-
  text-align:center;line-height:normal'><span style='font-size:12.0pt;
+
</tr>
-
  font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:
+
-
  "Times New Roman";color:black;mso-ansi-language:EN-US;mso-fareast-language:
+
-
  ES-MX'><a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a><o:p></o:p></span></p>
+
-
  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+
-
  text-align:center;line-height:normal'>&nbsp;</p>
+
-
  </td>
+
-
  <td width=400 valign=top style='width:300.0pt;border-top:none;border-left:
+
-
  none;border-bottom:solid #FABF8F 1.0pt;mso-border-bottom-themecolor:accent6;
+
-
  mso-border-bottom-themetint:153;border-right:solid #FABF8F 1.0pt;mso-border-right-themecolor:
+
-
  accent6;mso-border-right-themetint:153;mso-border-top-alt:solid #FABF8F .5pt;
+
-
  mso-border-top-themecolor:accent6;mso-border-top-themetint:153;mso-border-left-alt:
+
-
  solid #FABF8F .5pt;mso-border-left-themecolor:accent6;mso-border-left-themetint:
+
-
  153;mso-border-alt:solid #FABF8F .5pt;mso-border-themecolor:accent6;
+
-
  mso-border-themetint:153;padding:0cm 5.4pt 0cm 5.4pt;height:75.0pt'>
+
-
  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align:
+
-
  justify;line-height:normal'><span style='font-size:12.0pt;font-family:Oxygen;
+
-
  mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Arial;
+
-
  color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>This
+
-
  specific is <span class=SpellE>RBS</span> based on <span class=SpellE>Elowitz</span>
+
-
  <span class=SpellE>repressilator</span>. It is very common to see in many <span
+
-
  class=SpellE>iGEM</span> projects.<span style='mso-spacerun:yes'>  </span>It
+
-
  has a length of 12 <span class=SpellE>pb</span>. <b>(Mahajan, <span
+
-
  class=SpellE>Marinescu</span>, Chow, <span class=SpellE>Wissner</span>-Gross,
+
-
  &amp; Carr, 2003)</b>.<o:p></o:p></span></p>
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  </v:shape><![endif]--><![if !vml]><img width=76 height=64
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  v:shapes="_x0033__x0020_Imagen"><![endif]></p>
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  mso-bidi-font-family:"Times New Roman";color:black;mso-ansi-language:EN-US;
+
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  mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_K729001">BBa_K729001</a><o:p></o:p></span></p>
+
-
  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
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  text-align:center;line-height:normal;mso-yfti-cnfc:64'><span
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+
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+
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  <p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;text-align:
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  justify;line-height:normal;mso-yfti-cnfc:64'><span style='font-size:12.0pt;
+
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  font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:
+
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  Arial;color:#282828;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>Gene
+
-
  that produces <span class=SpellE>IrrE</span>, a substance that changes the
+
-
  bacteria’s metabolism and allows bacteria to survive to extreme conditions,
+
-
  some examples could be high UV rays exposition, or high salt concentration
+
-
  levels in an aquatic environment, oxidative or thermal shock.<span
+
-
  style='mso-spacerun:yes'>  </span>It has a length of 933pb <b>(<span
+
-
  class=SpellE>Sohrabi</span>, 2012)</b>.<o:p></o:p></span></p>
+
-
  </td>
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+
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  solid #FABF8F .5pt;mso-border-themecolor:accent6;mso-border-themetint:153;
+
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+
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  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+
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  text-align:center;line-height:normal'><span lang=ES-MX style='font-size:12.0pt;
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  font-family:Oxygen;mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:
+
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  "Times New Roman";color:black;mso-fareast-language:ES-MX'><a href="http://parts.igem.org/Part:BBa_B1002">BBa_B1002</a><o:p></o:p></span></p>
+
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  <p class=MsoNormal align=center style='margin-bottom:0cm;margin-bottom:.0001pt;
+
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  text-align:center;line-height:normal'>&nbsp;</p></td>
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+
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+
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  color:black;mso-ansi-language:EN-US;mso-fareast-language:ES-MX'>Part made of
+
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  6pb responsible for transcription stop <b>(Huang, 206)</b>.<o:p></o:p></span></p>
+
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  </td>
+
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</tr>
+
</table>
</table>
</div></center>
</div></center>
 +
<p><b><h2>Justifications</h2></b></p>
 +
 +
<p>The resistance module is very important in our project because it allows the correct function of the bio-filter. Because the objective is to remove the sodium ions from the water, E. CARU, at the beggining of the process, will be surrounded by salt molecules which normally <i>E. coli</i>  could not support.</p>
 +
 +
<p>IrrE gives resistance to the high salt concentration of the water, allowing the Nhas gene to function and capture the sodium ions.</p>
 +
 +
<p>Another important aspect in the usage of the IrrE gene, is because the promoter in the <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_capture">Capture module</a> is activated with UV rays at 360 wv, intensity that can cause mutations to the bacterium. IrrE protects <i>E. coli </i>  from UV rays and allows the bacteria to their work. </p>
 +
<br>
 +
 +
<p><b><h2>Other teams that used IRRE</h2></b></p>
 +
 +
<p><b><a href="https://2012.igem.org/Team:University_College_London">UCL 2012</a>:</b> They propose to confer salt tolerance on <i>E. coli</i> by linking the salt tolerance gene encoding the protein irrE (<a href="http://parts.igem.org/Part:BBa_K729001">BBa_K729001</a>) to a constitutive promoter (<a href="http://parts.igem.org/Part:BBa_J23119">BBa_J23119</a>). </p><center>
 +
 +
<p><img width=661 height=139 src="https://static.igem.org/mediawiki/2014hs/3/33/Gen1cideb2014.png"align=center hspace=12 alt="IMG_0317"></p>
 +
 +
<p><b>Image 3.</b> Part designed by <a href="https://2012.igem.org/Team:University_College_London">UCL 2012</a> for the irrE protein.</p></center>
 +
<br>
 +
 +
 +
<p><h2><b>Project Zoom In</b></h2></p></br><center><iframe width="600" height="500" src="//www.youtube.com/embed/qAYmPrq9NsY" frameborder="0" allowfullscreen></iframe></center>
 +
<br>
 +
 +
<p><b><h2>Bibliography/References</h2></b></p><font size="2">
-
<br><p><b>Bibliography</b></p>
+
<p>● Antiquity 2013. (2013, January 31). <i>Part:BBa_B0034</i>. Retrieved from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034">http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034</a>.</p>
-
<font size="2">
 
-
<p>● Antiquity 2013. (2013, January 31). <i>Part:BBa_B0034</i> Retrieved from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034">http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034</a>.</p>
 
<p>● Huang, H. (2006, August 30). <i>Part:BBa_B1002</i>. Retrieved August 30, 2014, from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B1002">http://parts.igem.org/wiki/index.php?title=Part:BBa_B1002</a>.</p>
<p>● Huang, H. (2006, August 30). <i>Part:BBa_B1002</i>. Retrieved August 30, 2014, from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B1002">http://parts.igem.org/wiki/index.php?title=Part:BBa_B1002</a>.</p>
 +
<p>● iGEM2006_Berkeley. (2006, August 24). <i>Part:BBa_J23119</i>. Retrieved April 30, 2014, from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_J23119">http://parts.igem.org/wiki/index.php?title=Part:BBa_J23119</a>.</p>
<p>● iGEM2006_Berkeley. (2006, August 24). <i>Part:BBa_J23119</i>. Retrieved April 30, 2014, from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_J23119">http://parts.igem.org/wiki/index.php?title=Part:BBa_J23119</a>.</p>
 +
<p>● Sohrabi, B. (2012, June 27). <i>Part:BBa_K729001</i>. Retrieved from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K729001">http://parts.igem.org/wiki/index.php?title=Part:BBa_K729001</a>.</p>
<p>● Sohrabi, B. (2012, June 27). <i>Part:BBa_K729001</i>. Retrieved from <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K729001">http://parts.igem.org/wiki/index.php?title=Part:BBa_K729001</a>.</p>
-
<p>● UCLiGEM Team. (2012). <i>IRRE module</i>. Retrieved March 31, 2014 from <a href="https://2012.igem.org/Team:University_College_London/Module_5">https://2012.igem.org/Team:University_College_London/Module_5</a>.</p>
 
-
</font>
 
 +
<p>● UCLiGEM Team. (2012). <i>IRRE module</i>. Retrieved March 31, 2014. from <a href="https://2012.igem.org/Team:University_College_London/Module_5">https://2012.igem.org/Team:University_College_London/Module_5</a>.</p></font>
<br>
<br>
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<div style="text-align: right;"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_resistance#"><font color="blue">Return to the Top</font></a></p></div>
 
 +
<div style="text-align: right;"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_resistance#"><font color="blue">Return to the Top</font></a></p>
 +
</div>
</div>
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Latest revision as of 15:26, 19 June 2014

Description Capture Module Aroma Module Resistance Module Union Module Parts
iGEM CIDEB 2014 - Project

Resistance Module

Because our project is a biofilter which allows to remove salt from water, the bacteria needs to survive to extreme environmental conditions which normally it can’t do. We need E. coli to survive a high salinity environment to allow it to capture sodium ions and to remove the salt concentration of the water.

In order to accomplish our project goal, we have to change E. coli metabolism and make it stronger, more resistant and more efficient than in normal E. coli bacteria.

Last discoveries show irrE as a protein capable of change the E. coli metabolism, and giving it the ability to survive to bigger temperatures, bigger UV rays radiation and bigger salt concentration (UCL, 2012).


Description

The protein irrE originates from Deinococcus radiodurans, and initially, this gene provides resistance to radiation. But when transformed in E. coli, it protects it against salt, oxidative and thermal shock. (UCL, 2012) Also, different experiments from different iGEM teams, support the idea of the bigger salt resistance in E. coli with this biobrick.

IMG_0317

Image 1. This graph done by UCL iGEM 2012, shows how irrE biobrick increased the salt concentration resistance in E. coli compared with the results from Tu Delf iGEM 2010.


irrE has been demonstrated to up regulate transcription of recA and pprA - genes which encode Recombines A and Radiation Inducible Protein. With respect to salt tolerance, irrE up regulates the production of several stress responsive proteins, protein kinases, metabolic proteins, and detoxification proteins. It also down regulates glycerol degradation. With this global regulatory effect, E. coli becomes more salt tolerant (UCL, 2012).

IMG_0317

Image 2. This diagram shows the effect of irrE protein on E. coli metabolism.

Device

Initially, IrrE and L2+AIDA, protein for binding silica (Union module), were joined together in only one circuit, but we needed to separate them because L2+AIDA has not been proved yet and it could affect the correct production of IrrE (see image 3).

IMG_0317

Image 3. Circuit for our project and for testing resistance and union modules.


Parts of the Module


IMAGE

CODE

DESCRIPTION

 

BBa_J23119

 

In the specific case of our bacteria, it helps to continuously transcribing the irrE gene in order to make bacteria resist high concentration of salt. This promoter has a length of 35 pb (Anderson, 2006).

 

BBa_B0034

 

This specific is RBS based on Elowitz repressilator . It is very common to see in many iGEM projects. It has a length of 12 pb (Mahajan, Marinescu , Chow, Wissner -Gross, & Carr, 2003).

BBa_K729001

Gene that produces irrE , a substance that changes the bacteria’s metabolism and allows bacteria to survive to extreme conditions, some examples could be high UV rays exposition, or high salt concentration levels in an aquatic environment, oxidative or thermal shock. It has a length of 933pb ( Sohrabi , 2012).

BBa_B1002

 

Part made of 6pb responsible for transcription stop (Huang, 206).

Justifications

The resistance module is very important in our project because it allows the correct function of the bio-filter. Because the objective is to remove the sodium ions from the water, E. CARU, at the beggining of the process, will be surrounded by salt molecules which normally E. coli could not support.

IrrE gives resistance to the high salt concentration of the water, allowing the Nhas gene to function and capture the sodium ions.

Another important aspect in the usage of the IrrE gene, is because the promoter in the Capture module is activated with UV rays at 360 wv, intensity that can cause mutations to the bacterium. IrrE protects E. coli from UV rays and allows the bacteria to their work.


Other teams that used IRRE

UCL 2012: They propose to confer salt tolerance on E. coli by linking the salt tolerance gene encoding the protein irrE (BBa_K729001) to a constitutive promoter (BBa_J23119).

IMG_0317

Image 3. Part designed by UCL 2012 for the irrE protein.


Project Zoom In



Bibliography/References

● Antiquity 2013. (2013, January 31). Part:BBa_B0034. Retrieved from http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034.

● Huang, H. (2006, August 30). Part:BBa_B1002. Retrieved August 30, 2014, from http://parts.igem.org/wiki/index.php?title=Part:BBa_B1002.

● iGEM2006_Berkeley. (2006, August 24). Part:BBa_J23119. Retrieved April 30, 2014, from http://parts.igem.org/wiki/index.php?title=Part:BBa_J23119.

● Sohrabi, B. (2012, June 27). Part:BBa_K729001. Retrieved from http://parts.igem.org/wiki/index.php?title=Part:BBa_K729001.

● UCLiGEM Team. (2012). IRRE module. Retrieved March 31, 2014. from https://2012.igem.org/Team:University_College_London/Module_5.
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