Team:CIDEB-UANL Mexico/achievements

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       <h2>Abstract</h2>
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       <h2>Achievements</h2>
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<p>Since always, water has been known as a source for life. We cannot survive without water. Although useful water is a necessity which must be satisfied for everyone, nowadays in certain countries there are no enough water supplies for people.  The global lack of abundance of usable water is an issue that presents a dangerous problem to our future. Ironically, only a small portion of our planet's water is actually usable. Ninety-seven percent of the world's water is too salty for consumption or agricultural use. Furthermore, much of the rest is held in ice caps or other unattainable sources. This leaves approximately one percent of the global water as liquid and fresh; ninety-eight percent of which is groundwater (Bouwer, 2).</p>
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<p>During the project, iGEM CIDEB 2014 acquired the following achievements:</p>
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<p>In fact, for solving this problem have been developed different methods. One of them is desalination, converting sea water (rich in salts) into usable water; but this method is very expensive by the great use of electrical energy, and the extraction process produces wastes dangerous for the environment (Cotruvo, 2-3).</p>
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<p><h2><b>Dry Lab <img width=15 height=20 src="https://static.igem.org/mediawiki/2014hs/c/cf/Check1.png"/></h2></b></p>
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<p>For that reason our project is focused on developing a biological machine capable of performing desalination, reducing costs and avoiding dangerous wastes during the process.  For making this possible, E. coli must survive in saline environments, able to capture salts, and be removed from the water after the process. In order to achieve the objective we designed a biological circuit in which E. coli could be able to resist adverse conditions though a protein called IrrE, capture Na+ ions (this because sodium chloride is the main salt of sea water) by NhaS production releasing an aroma (WinterGreen) as reporter, and be able for binding silica (L2+AIDA) in order to remove it through a biofilter.  The whole circuit is shown below:</p>
 
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<center><p><img width=552 height=343 src="https://static.igem.org/mediawiki/2014hs/9/9b/Whole_circuit.png"
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<p><b><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> Cloned BioBricks in <i>E. coli</i> and <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/labwork_results#Characterization">characterized</a> them:</b></p>
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align=center hspace=12 alt="IMG_0317"></p>
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<p>-> <a href="http://parts.igem.org/Part:BBa_K1255003" target="_blank">BBa_K1255003</a> which gives our bacteria the ability to produce an odor.</p>
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<p>-> <a href="http://parts.igem.org/Part:BBa_K1255002" target="_blank">BBa_K1255002</a> which gives our bacteria the ability capture Na ions.</p>
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<p><b>Figure 1.</b> Diagram representing our proposed circuit</p></center>
 
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<p>But we realized E. coli could have a genetic overload because the circuit was too big (approximately 5000 bp). Also the time we had to finish it was not enough, as well as most of the proteins we wanted to produce were putative or untested. So for a better understanding and for determine if each piece works we divided the project into four modules: capture, binding, aroma and resistance, but the project is the result of their correlation.  In fact our bacteria was named E. CARU (each letter by each module). </p>
 
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<p><b>E</b>scherichia coli</p>
 
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<p><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_capture"><b>C</b>apture</a></p>
 
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<p><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_aroma"><b>A</b>roma</a></p>
 
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<p><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_resistance"><b>R</b>esistance </a></p>
 
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<p><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_union"><b>U</b>nion</a></p>
 
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<td style="padding-left:12px;"><img width=286 height=285 src="https://static.igem.org/mediawiki/2014hs/3/30/Image006cideb2014.png"/></td>
 
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<p><b>Future results</b></p>
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<p><b><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> Characterized a part from another team:</b></p>
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<p>●From <a href="https://2006.igem.org/wiki/index.php/MIT_2006" target="_blank">MIT 2006</a>:</p>
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<p>-> <a href="http://parts.igem.org/Part:BBa_J45004" target="_blank">BSMT1 (BBa_J45004)</a> which gives our bacteria the ability to produce an odor. </p>
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<p>●From <a href="https://2008.igem.org/Team:TUDelft" target="_blank">Tu Delft 2008</a>:</p>
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<p>-> RNA Thermomether: <a href="http://parts.igem.org/Part:BBa_K115017" target="_blank">BBa_K115017</a> which gives our Aroma module the ability to be regulated by temperature. </p>
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<p>●From <a href="https://2007.igem.org/wiki/index.php/Colombia-Israel_(ORT_Ebin_High_School)" target="_blank">Colombia-Israel 2007</a>:</p>
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<p>-> UV Promoter: <a href="http://parts.igem.org/Part:BBa_I765001" target="_blank">BBa_I765001</a> which lets our Capture module the ability to be regulated by UV light. </p>
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<p><b><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> Created a completely new part in the registry.</b></p>
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<p>-> nhaS gene, which gives our bacteria the ability to bind to Sodium ions: <a href="http://parts.igem.org/Part:BBa_K1255000" target="_blank">BBa_K1255000</a></p>
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<p><b><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> Finished a construction of the project from another team.</b></p>
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<p>->From the <a href="https://2012.igem.org/Team:UANL_Mty-Mexico" target="_blank">UANL 2012</a> team:</p>
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<p>- AIDA-I Signal peptide: <a href="http://parts.igem.org/Part:BBa_K888005" target="_blank">BBa_K888005</a></p>
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<p>- L2 protein of adhesion to silica: <a href="http://parts.igem.org/Part:BBa_K888000" target="_blank">BBa_K888000</a></p>
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<p>- AIDA-I Translocator domain: <a href="http://parts.igem.org/Part:BBa_K888001" target="_blank">BBa_K888001</a></p>
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<p><b><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> Used an already existent part from the registry.</b></p>
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<p> -> Which gives our bacteria the resistance to a saline environment: <a href="http://parts.igem.org/Part:BBa_K729001" target="_blank">IrrE (BBa_K729001)</a> from <a href="https://2012.igem.org/Team:University_College_London" target="_blank">UCL 2012</a>.</p>
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<br><p><h2><b>Lab Work <img width=15 height=20 src="https://static.igem.org/mediawiki/2014hs/c/cf/Check1.png"/></b></h2></p>
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<p><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> Obtained the <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/labwork_results" target="_blank">experimental results</a> that were needed for the correct function of an important part of our project. </p>
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<p><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> We had a control over our experiments, being regulated by heat (<a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_aroma" target="_blank">Aroma module</a>) and UV light (<a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_capture" target="_blank">Capture module</a>).</p>
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<p><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> All the lab work was performed following the correct <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/labwork_methods" target="_blank">methods</a> and the <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/safety_riskanalysis" target="_blank">safety</a> protocols.</p>
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<br><p><h2><b>Math Model <img width=15 height=20 src="https://static.igem.org/mediawiki/2014hs/c/cf/Check1.png"/></b></h2></p>
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<p><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> Mathematically modeled all the four modules of our project:</p>
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<p> -> <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/math_capture" target="_blank">Capture</a></p>
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<p> -> <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/math_aroma" target="_blank">Aroma</a></p>
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<p> -> <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/math_resistance" target="_blank">Resistance</a></p>
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<p> -> <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/math_union" target="_blank">Union</a></p>
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<p><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> Graphs were done with the correct information from the model.</p>
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<br><p><h2><b>Human Practices <img width=15 height=20 src="https://static.igem.org/mediawiki/2014hs/c/cf/Check1.png"/></b></h2></p>
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<p><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> Achieved that all students from our high school knew what iGEM is and the explanation of this year’s project.  <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/hp_explosion" target="_blank">Explosion</a> & <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/hp_dnaweek" target="_blank">DNA Week</a></p>
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<p><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> Gave a presentation to 40 foreign teachers in which it was explained what iGEM is and our project. <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/hp_cinvestav" target="_blank">CINVESTAV</a></p>
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<p><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> Organized a 4k race for students and general public to promote: health, science and iGEM’s work. <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/hp_race" target="_blank">Race 4 Science</a></p>
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<p><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> Collaborated with the HS teams UCL Academy and GenetiX Tec CCM. <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/hp_collaborations" target="_blank"> Collaborations</a></p>
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<p><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> Collaborated with the College team Evry. <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/hp_collaborations" target="_blank">Collaborations</a></p>
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<p><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> Created a videogame to recreate the main function of our project. <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/hp_videogame" target="_blank">Videogame</a></p>
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<p><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> Participated in an event with all the iGEM teams from the state. <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/hp_enlacecientifico" target="_blank">Enlace Científico</a></p>
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<br><p><h2><b>Safety <img width=15 height=20 src="https://static.igem.org/mediawiki/2014hs/c/cf/Check1.png"/></b></h2></p>
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<p><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> Performed a complete Risk Analysis from our project. <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/safety_riskanalysis" target="_blank">Risk Analysis</a></p>
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<p><img width=18 height=21 src="https://static.igem.org/mediawiki/2014hs/1/13/Palomitacideb2014.gif"/> Designed a contention method for our bacteria. <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/safety_riskanalysis" target="_blank">Risk Analysis</a></p>
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<p>Once we have proved each piece works alone, and we obtained experimental data to support their effectiveness we planned to join every module into the whole circuit we propose at first. It would mean to place IrrE and L2+AIDA gene in E. coli. In the case of NhaS and Wintergreen we would replace the RFP gene from NhaS with the Wintergreen reporter.</p>
 
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<div style="text-align: right;"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_abstract#"><font color="blue">Return to the Top</font></a></p></div>
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<div style="text-align: right;"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/achievements#"><font color="blue">Return to the Top</font></a></p></div>
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Latest revision as of 03:16, 21 June 2014

iGEM CIDEB 2014 - Achievements

Achievements

During the project, iGEM CIDEB 2014 acquired the following achievements:

Dry Lab

Cloned BioBricks in E. coli and characterized them:

-> BBa_K1255003 which gives our bacteria the ability to produce an odor.

-> BBa_K1255002 which gives our bacteria the ability capture Na ions.

Characterized a part from another team:

●From MIT 2006:

-> BSMT1 (BBa_J45004) which gives our bacteria the ability to produce an odor.

●From Tu Delft 2008:

-> RNA Thermomether: BBa_K115017 which gives our Aroma module the ability to be regulated by temperature.

●From Colombia-Israel 2007:

-> UV Promoter: BBa_I765001 which lets our Capture module the ability to be regulated by UV light.

Created a completely new part in the registry.

-> nhaS gene, which gives our bacteria the ability to bind to Sodium ions: BBa_K1255000

Finished a construction of the project from another team.

->From the UANL 2012 team:

- AIDA-I Signal peptide: BBa_K888005

- L2 protein of adhesion to silica: BBa_K888000

- AIDA-I Translocator domain: BBa_K888001

Used an already existent part from the registry.

-> Which gives our bacteria the resistance to a saline environment: IrrE (BBa_K729001) from UCL 2012.


Lab Work

Obtained the experimental results that were needed for the correct function of an important part of our project.

We had a control over our experiments, being regulated by heat (Aroma module) and UV light (Capture module).

All the lab work was performed following the correct methods and the safety protocols.


Math Model

Mathematically modeled all the four modules of our project:

-> Capture

-> Aroma

-> Resistance

-> Union

Graphs were done with the correct information from the model.


Human Practices

Achieved that all students from our high school knew what iGEM is and the explanation of this year’s project. Explosion & DNA Week

Gave a presentation to 40 foreign teachers in which it was explained what iGEM is and our project. CINVESTAV

Organized a 4k race for students and general public to promote: health, science and iGEM’s work. Race 4 Science

Collaborated with the HS teams UCL Academy and GenetiX Tec CCM. Collaborations

Collaborated with the College team Evry. Collaborations

Created a videogame to recreate the main function of our project. Videogame

Participated in an event with all the iGEM teams from the state. Enlace Científico


Safety

Performed a complete Risk Analysis from our project. Risk Analysis

Designed a contention method for our bacteria. Risk Analysis

iGEM CIDEB 2014 - Footer

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