Team:Nanjing NFLS

From 2014hs.igem.org

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===Project===
===Project===
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CRISPRs (ClusteredRegularly Interspaced Short Palindromic Repeats) are DNA loci thatcontain multiple, short, direct repetitions of base sequences,which are often associated with cas genes which code for proteinsthat perform various functions related to CRISPRs. The CRISPR-Cassystem is a novel technique of gene editing, including silencing,enhancing or changing specific genes. By inserting a plasmidcontaining cas genes and specifically designed CRISPRs, theorganism's genome can be cut at any desired location.
 
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Based on the CRISPR-Cassystem, the team plans to design a set of biological machine repairkit including tools to delete and/or replace biobricks in buildingcomplex synthetic biology devices and systems. The proceduresinclude choosing the ideal promoter and terminator withuniversality for knock-in and designing the DNA sequences for adonor plasmid with homogenous arms and guide-RNA, which then aretransfected into the host cells with Cas9. The proof of principlekits will be tested on basic PRCT protein generators with EGFP andRFP as reporters and applied to replace causative genes withnon-causative alleles once with prove validity.
 
===Notebook===
===Notebook===

Revision as of 13:21, 4 March 2014


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have the following information on your wiki:
  • a team description
  • project description
  • safety information (did your team take a safety training course? were you supervised in the lab?)
  • team attribution (who did what part of your project?)
You may also wish to add other page such as:
  • lab notebook
  • sponsor information
  • other information
REMEMBER, keep all of your pages within your teams namespace.
Example: 2013hs.igem.org/Team:Nanjing_NFLS/Our_Pets



You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Nanjing NFLS logo.png

Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)

File:Nanjing NFLS team.png
Your team picture
Team Nanjing_NFLS


Official Team Profile

Contents

Team

Tell us about your team, your school!


Project

Notebook

Show us how you spent your days.


Results/Conclusions

What did you achieve over the course of your semester?


Safety

What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?


Attributions

Who worked on what?


Human Practices

What impact does/will your project have on the public?


Fun!

What was your favorite team snack?? Have a picture of your team mascot?


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