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Team:Nanjing NFLS
From 2014hs.igem.org
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| - | + | CRISPRs (ClusteredRegularly Interspaced Short Palindromic Repeats) are DNA loci thatcontain multiple, short, direct repetitions of base sequences,which are often associated with cas genes which code for proteinsthat perform various functions related to CRISPRs. The CRISPR-Cassystem is a novel technique of gene editing, including silencing,enhancing or changing specific genes. By inserting a plasmidcontaining cas genes and specifically designed CRISPRs, theorganism's genome can be cut at any desired location. | |
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| + | Based on the CRISPR-Cassystem, the team plans to design a set of biological machine repairkit including tools to delete and/or replace biobricks in buildingcomplex synthetic biology devices and systems. The proceduresinclude choosing the ideal promoter and terminator withuniversality for knock-in and designing the DNA sequences for adonor plasmid with homogenous arms and guide-RNA, which then aretransfected into the host cells with Cas9. The proof of principlekits will be tested on basic PRCT protein generators with EGFP andRFP as reporters and applied to replace causative genes withnon-causative alleles once with prove validity. | ||
===Notebook=== | ===Notebook=== | ||
Revision as of 13:16, 4 March 2014
- a team description
- project description
- safety information (did your team take a safety training course? were you supervised in the lab?)
- team attribution (who did what part of your project?)
- lab notebook
- sponsor information
- other information
Example: 2013hs.igem.org/Team:Nanjing_NFLS/Our_Pets
| You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing. |  |
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Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs) | File:Nanjing NFLS team.png Your team picture |
| Team Nanjing_NFLS |
| Official Team Profile |
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Contents |
Team
Tell us about your team, your school!
Project
CRISPRs (ClusteredRegularly Interspaced Short Palindromic Repeats) are DNA loci thatcontain multiple, short, direct repetitions of base sequences,which are often associated with cas genes which code for proteinsthat perform various functions related to CRISPRs. The CRISPR-Cassystem is a novel technique of gene editing, including silencing,enhancing or changing specific genes. By inserting a plasmidcontaining cas genes and specifically designed CRISPRs, theorganism's genome can be cut at any desired location.
Based on the CRISPR-Cassystem, the team plans to design a set of biological machine repairkit including tools to delete and/or replace biobricks in buildingcomplex synthetic biology devices and systems. The proceduresinclude choosing the ideal promoter and terminator withuniversality for knock-in and designing the DNA sequences for adonor plasmid with homogenous arms and guide-RNA, which then aretransfected into the host cells with Cas9. The proof of principlekits will be tested on basic PRCT protein generators with EGFP andRFP as reporters and applied to replace causative genes withnon-causative alleles once with prove validity.
Notebook
Show us how you spent your days.
Results/Conclusions
What did you achieve over the course of your semester?
Safety
What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
Attributions
Who worked on what?
Human Practices
What impact does/will your project have on the public?
Fun!
What was your favorite team snack?? Have a picture of your team mascot?
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